FastQCFastQC Report
Thu 26 May 2016
SRR1512980_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512980_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2596954
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC38990.15013743023557596No Hit
GTATCAACGCAGAGTACTTTTTTTT37170.14312921984755989No Hit
CCCATGTACTCTGCGTTGATACCAC31000.11937061649917559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC1251.4242687E-912.15318310
TAGGACC6850.011.9235364
TGTAGGA16400.010.8870752
AGGACGT13850.010.7656125
GTAGGAC14850.010.7443473
GGACGTG13350.010.596446
AGGCGTG2200.010.3572287
TAGGACG14350.010.2583374
GTGCGCC1951.4551915E-1110.22503411
CTGTAGG16450.010.1967331
TTAGGAC10250.010.1921553
GTCCTAC13400.010.0994531
CCTACAC4150.010.0693583
TGGCGAG13100.010.01050818
CCTAGAC1901.0004442E-109.9970893
GGCGAGG6450.09.86249519
GCGTGCG1651.0322765E-89.78239259
GTCTTAA4200.09.7573861
GACGTGA7600.09.743977
GTCCTAG3550.09.6647021
Produced by FastQC (version 0.11.5)