##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512980_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2596954 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.958643472314105 31.0 30.0 33.0 23.0 34.0 2 29.074255839726078 31.0 30.0 34.0 23.0 34.0 3 29.226300504360108 31.0 30.0 34.0 23.0 34.0 4 32.743988919326256 35.0 33.0 37.0 26.0 37.0 5 32.83327043913754 35.0 33.0 37.0 26.0 37.0 6 32.962082886335295 36.0 35.0 37.0 26.0 37.0 7 32.87515027220351 37.0 35.0 37.0 26.0 37.0 8 32.88144572449108 37.0 35.0 37.0 26.0 37.0 9 34.22822622195079 39.0 35.0 39.0 25.0 39.0 10 34.08802620300552 38.0 34.0 39.0 25.0 39.0 11 34.171681131048146 39.0 35.0 39.0 25.0 39.0 12 34.0526077858907 39.0 35.0 39.0 25.0 39.0 13 34.057258619136114 39.0 35.0 39.0 25.0 39.0 14 35.06838010992879 39.0 35.0 41.0 25.0 41.0 15 34.99551936614973 39.0 35.0 41.0 24.0 41.0 16 34.87839907830482 39.0 34.0 41.0 23.0 41.0 17 34.830401693676514 39.0 34.0 41.0 22.0 41.0 18 34.511052563888306 39.0 34.0 41.0 18.0 41.0 19 34.756477396211096 39.0 34.0 41.0 20.0 41.0 20 34.83333320497783 39.0 34.0 41.0 22.0 41.0 21 34.77513502356992 39.0 34.0 41.0 22.0 41.0 22 34.692381151148616 39.0 34.0 41.0 19.0 41.0 23 34.41915105157812 39.0 34.0 41.0 18.0 41.0 24 34.41998472056109 39.0 34.0 41.0 18.0 41.0 25 34.34279736953369 39.0 34.0 41.0 17.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5518330135264691 1101 2 0.8564601168509327 1101 3 0.8978815543129279 1101 4 0.9031007366360839 1101 5 0.7773682526590093 1101 6 0.976590614929151 1101 7 1.015118068131521 1101 8 0.781775226829339 1101 9 0.7745468843584149 1101 10 0.868168461234113 1101 11 1.0079892515950704 1101 12 1.2823609914714424 1101 13 1.09565218684984 1101 14 1.1017872254618197 1101 15 0.7706665802143675 1101 16 1.247424852139602 1101 17 1.295888340008311 1101 18 1.506969493888576 1101 19 0.9314545347512251 1101 20 1.124058150613358 1101 21 1.1775223430100326 1101 22 1.1962709565839305 1101 23 1.4587942370030689 1101 24 1.1884422533195291 1101 25 1.3712445473726405 1102 1 0.4355523786479054 1102 2 0.5304228979947823 1102 3 0.5132235651601462 1102 4 0.6031529004603726 1102 5 0.4691847685183035 1102 6 0.5054907377707352 1102 7 0.8464881448051997 1102 8 0.7553423294504569 1102 9 0.6956065180683311 1102 10 0.8972309870155755 1102 11 0.8266850130732237 1102 12 0.8807027275328849 1102 13 0.7459786492395892 1102 14 0.8092836802056382 1102 15 1.061709278545706 1102 16 1.0252981011923055 1102 17 0.7902571155096325 1102 18 1.1281493057740022 1102 19 1.176402991306162 1102 20 1.0164617206197448 1102 21 0.729724884161314 1102 22 1.174921985796324 1102 23 1.3542442263169505 1102 24 1.2751291748808598 1102 25 1.0173749075628749 1103 1 0.5255999371114939 1103 2 0.7130970013511408 1103 3 0.6042082828385666 1103 4 0.8781002680842676 1103 5 0.4372903058986495 1103 6 0.8496333980154915 1103 7 0.6831145988219234 1103 8 0.6886290888106856 1103 9 1.012373148384576 1103 10 0.98719334918691 1103 11 1.0349380725667388 1103 12 1.1266174706565977 1103 13 1.0419289854809577 1103 14 1.2058398567475024 1103 15 1.2842454017736245 1103 16 1.4095473893299584 1103 17 1.2078802525507086 1103 18 1.4956221186905196 1103 19 1.5294867184356917 1103 20 1.4502713958893452 1103 21 1.3070808456298693 1103 22 1.4387605142102373 1103 23 1.7640754997932646 1103 24 1.6141358239781596 1103 25 1.5074360974332208 1104 1 0.589829600669578 1104 2 0.5309643743257482 1104 3 0.5695801948905022 1104 4 0.8264635352571119 1104 5 0.6764549781222513 1104 6 0.6714208832291035 1104 7 0.9359038102603989 1104 8 0.8997877398794358 1104 9 0.9755344094227851 1104 10 0.9743889387167712 1104 11 0.8753568368373266 1104 12 0.9737722084904945 1104 13 0.9481390106522056 1104 14 1.0807852674652239 1104 15 1.1963304372609542 1104 16 1.0836773135411164 1104 17 1.1203508517089205 1104 18 1.2521629463156216 1104 19 1.2776367005544813 1104 20 1.157821566448618 1104 21 1.3157902002555986 1104 22 1.1540103525915697 1104 23 1.447032983768949 1104 24 1.2582407832640072 1104 25 0.9508179851392029 1105 1 0.5430963296078843 1105 2 0.7341254143632483 1105 3 0.8079136203754231 1105 4 0.935801700106353 1105 5 0.6708369524887772 1105 6 0.5173068326726238 1105 7 0.7364478145192663 1105 8 0.8676210660363637 1105 9 0.7250472496456339 1105 10 0.9289821612202829 1105 11 0.9028877557338149 1105 12 0.8808004103014895 1105 13 0.9162202286580694 1105 14 0.9924530774639848 1105 15 1.2511985120691236 1105 16 1.1610286224687982 1105 17 1.0487364072655652 1105 18 0.9996344861539725 1105 19 1.0116805511730007 1105 20 1.1259038853805308 1105 21 1.115163614310461 1105 22 0.8751419920319279 1105 23 1.0680508119207488 1105 24 1.166988692727763 1105 25 1.1647663879156838 1106 1 0.6514727785131562 1106 2 0.7028629238005628 1106 3 0.668710462761176 1106 4 0.7121512360125593 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1.2918907770503694 1107 18 1.4690240338043878 1107 19 1.3823162560478366 1107 20 1.2406052468210262 1107 21 1.342661418438702 1107 22 1.2776343064807634 1107 23 1.3028004825399861 1107 24 1.2573488156965809 1107 25 1.36556239142908 1108 1 0.39428995187708793 1108 2 0.39908006705804056 1108 3 0.3994106176484564 1108 4 0.4770470657956025 1108 5 0.4788070094793113 1108 6 0.5939290329361242 1108 7 0.6972515035838285 1108 8 0.641477233882668 1108 9 0.7506068139438256 1108 10 0.7604895378173993 1108 11 0.6467989991079008 1108 12 0.7050077899626146 1108 13 0.7109662224687767 1108 14 0.8231513646840085 1108 15 0.8458629266905717 1108 16 0.8012567377980275 1108 17 0.8122199535473911 1108 18 0.9780475985723953 1108 19 0.9402240753939779 1108 20 0.8438668185545382 1108 21 0.7653449652311934 1108 22 0.63639455813788 1108 23 0.8159050494425841 1108 24 0.865029834683213 1108 25 0.9529879615897414 1109 1 -0.09126163052441072 1109 2 -0.061724339427058794 1109 3 0.08081797169936067 1109 4 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16 1.027468607929734 1110 17 0.65702350518157 1110 18 1.0315442983070326 1110 19 0.9936826359296376 1110 20 0.8981703897831608 1110 21 0.8758477541746998 1110 22 0.9788966948660942 1110 23 0.9162786736322133 1110 24 1.1006504235990988 1110 25 1.0879083521351376 1111 1 0.023132345504556895 1111 2 0.24062528477081457 1111 3 0.19760751225213724 1111 4 0.029841258196164233 1111 5 0.05694410383113535 1111 6 0.17078881932459922 1111 7 0.20954658555103833 1111 8 0.018370812251184532 1111 9 0.17668902376602347 1111 10 0.13513537187278502 1111 11 -0.0717863732970514 1111 12 0.1574546036965998 1111 13 0.17772155698050085 1111 14 0.21845687884198384 1111 15 0.2509096289399011 1111 16 0.10375416729697662 1111 17 0.13298093738499972 1111 18 0.46091874715312997 1111 19 0.07938854702604914 1111 20 0.07667226050742215 1111 21 0.19200517676321738 1111 22 0.0023342640032453232 1111 23 0.3684757671033694 1111 24 0.3852223478823049 1111 25 -0.18548550737362746 1112 1 -0.05578663736438827 1112 2 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9 0.8299429950824191 2208 10 0.8332239563084443 2208 11 0.7646116754389283 2208 12 0.7973828813233865 2208 13 0.6803650969797843 2208 14 0.7701876779880337 2208 15 0.9047880222170122 2208 16 1.0735946836670038 2208 17 0.8808882258914892 2208 18 1.1138592940768746 2208 19 1.0285872216886176 2208 20 0.8131124389178552 2208 21 0.9276747170328505 2208 22 0.8369541783800187 2208 23 0.8689623454287698 2208 24 0.8984360436182044 2208 25 0.9010017878357459 2209 1 0.4719922732496329 2209 2 0.5121848023978508 2209 3 0.41300616238854104 2209 4 0.5214786604909207 2209 5 0.362573137030445 2209 6 0.547665870711711 2209 7 0.39007809127714665 2209 8 0.4065953782385989 2209 9 0.4718699283403893 2209 10 0.5661546988633361 2209 11 0.2660771142314502 2209 12 0.40800090466527905 2209 13 0.19707797128455695 2209 14 0.47805429327894444 2209 15 0.555971151550338 2209 16 0.6908270141282102 2209 17 0.5006942068589879 2209 18 0.9719430908931557 2209 19 0.5859158382902834 2209 20 0.7189536261029161 2209 21 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2215 16 -2.8231504383372865 2215 17 -2.962617372471694 2215 18 -1.9206322254770924 2215 19 -2.5395419804549917 2215 20 -2.876753932226009 2215 21 -2.452887712056693 2215 22 -2.1372933509786662 2215 23 -2.7977050363442366 2215 24 -2.5793173660598825 2215 25 -2.5453874870402977 2216 1 -2.1592992477876933 2216 2 -2.143355433226297 2216 3 -2.6636019380966545 2216 4 -3.322423916814916 2216 5 -2.667251736143392 2216 6 -2.6775880198309245 2216 7 -2.5118057679734456 2216 8 -3.1489875456809457 2216 9 -3.4711371476257185 2216 10 -2.9364244650559073 2216 11 -3.5429523178236515 2216 12 -3.9461316177174943 2216 13 -4.650658691481716 2216 14 -3.760503718971073 2216 15 -3.7221292764760534 2216 16 -2.972216070558506 2216 17 -2.9633311374106768 2216 18 -3.0773280636346634 2216 19 -3.462201398252425 2216 20 -3.187102585234637 2216 21 -3.560961303662282 2216 22 -4.080229457904739 2216 23 -4.129157018439255 2216 24 -4.1060285368112055 2216 25 -4.158318943321817 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 116798.0 3 3.0 4 943.0 5 5526.0 6 6885.0 7 6220.0 8 5639.0 9 5424.0 10 5008.0 11 4908.0 12 4688.0 13 4709.0 14 4661.0 15 4731.0 16 4893.0 17 5261.0 18 5782.0 19 7071.0 20 9084.0 21 11510.0 22 14621.0 23 17518.0 24 20458.0 25 23715.0 26 26500.0 27 29812.0 28 33826.0 29 40473.0 30 49500.0 31 62237.0 32 78345.0 33 100565.0 34 130926.0 35 172809.0 36 237318.0 37 379883.0 38 958704.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.60161864596061 18.35139084998234 12.760400364473565 25.28659013958348 2 15.343666684515112 19.71261948025583 40.339824132459604 24.60388970276945 3 18.92639393290386 24.032602013223237 29.271974656745904 27.769029397127003 4 12.675633157629553 16.43744539752145 37.531105115645545 33.35581632920345 5 13.87226121737027 37.36493641721371 35.23385082747571 13.528951537940303 6 32.79198033983191 36.23509013413728 17.77275652517852 13.200173000852294 7 28.579373261858258 31.352427842926865 21.703755368236678 18.364443526978196 8 25.851274721524774 35.78494752868523 20.296174930316198 18.067602819473798 9 26.525992107661178 15.245439720972007 19.73123220160512 38.4973359697617 10 15.24649860483388 28.4538401249391 34.0780638290907 22.221597441136314 11 34.504554606754034 22.458185890809602 24.03581447583894 19.001445026597423 12 23.405496776966547 24.536880306970975 30.98999525123947 21.067627664823007 13 28.82356248839769 21.612761345613617 25.50994313039339 24.053733035595307 14 21.502650344764366 21.49506252441006 27.172177273052917 29.830109857772662 15 23.692656954925955 29.570390091205006 23.129419132830222 23.607533821038817 16 22.97809226038482 27.745361522765457 26.555999497772714 22.72054671907701 17 22.492695638643443 27.970793338761375 27.081675585446586 22.4548354371486 18 22.50147444867184 27.145775752739777 28.329574934758057 22.023174863830327 19 23.730118532926348 27.006672893416656 27.11178300097253 22.151425572684467 20 23.997593592979825 26.8266624351699 27.194286555009583 21.981457416840687 21 23.51026690248908 26.514075393328362 26.87759214637423 23.09806555780833 22 23.701398862377225 27.09474066105982 26.57214591982105 22.631714556741912 23 23.074200374210157 26.94078189741034 26.935274275709233 23.04974345267027 24 23.329125078375217 26.673609388696406 27.27619349859271 22.721072034335663 25 23.25337139626298 26.66524675300666 27.0234732526011 23.057908598129256 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 254.0 1 254.0 2 453.5 3 653.0 4 653.0 5 653.0 6 1278.5 7 1904.0 8 1904.0 9 1904.0 10 2863.5 11 3823.0 12 3823.0 13 3823.0 14 6885.0 15 9947.0 16 9947.0 17 9947.0 18 18138.5 19 26330.0 20 26330.0 21 26330.0 22 41376.5 23 56423.0 24 56423.0 25 56423.0 26 81113.5 27 105804.0 28 105804.0 29 105804.0 30 135674.0 31 165544.0 32 165544.0 33 165544.0 34 193766.5 35 221989.0 36 221989.0 37 221989.0 38 247460.0 39 272931.0 40 272931.0 41 272931.0 42 292118.0 43 311305.0 44 311305.0 45 311305.0 46 329174.5 47 347044.0 48 347044.0 49 347044.0 50 341031.5 51 335019.0 52 335019.0 53 335019.0 54 305084.5 55 275150.0 56 275150.0 57 275150.0 58 238965.5 59 202781.0 60 202781.0 61 202781.0 62 167103.0 63 131425.0 64 131425.0 65 131425.0 66 102352.0 67 73279.0 68 73279.0 69 73279.0 70 53657.5 71 34036.0 72 34036.0 73 34036.0 74 23930.0 75 13824.0 76 13824.0 77 13824.0 78 9470.0 79 5116.0 80 5116.0 81 5116.0 82 3413.5 83 1711.0 84 1711.0 85 1711.0 86 1095.0 87 479.0 88 479.0 89 479.0 90 307.5 91 136.0 92 136.0 93 136.0 94 86.5 95 37.0 96 37.0 97 37.0 98 23.5 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35087259920660896 2 0.03939230344472794 3 0.03534910514394941 4 0.03534910514394941 5 0.04031646305633446 6 0.016750392960368184 7 0.016596366358433767 8 0.02244937723194173 9 0.019715405047605772 10 0.018829752086482857 11 0.017790072523425522 12 0.018752738785515647 13 0.018791245435999252 14 0.026492575532720258 15 0.027532255095777593 16 0.02002345825147461 17 0.02156372427081881 18 0.10643238193668429 19 0.024220683154187562 20 0.021255671066949974 21 0.025029322814343266 22 0.018752738785515647 23 0.021255671066949974 24 0.01809812572729436 25 0.021255671066949974 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2596954.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.42650236592628 #Duplication Level Percentage of deduplicated Percentage of total 1 79.53680265768232 53.62888412576427 2 13.01180546548083 17.546810640064315 3 3.8550786655690636 7.798034122944732 4 1.4768160432109183 3.983061617263954 5 0.6925847246909798 2.3349282788990373 6 0.3955369177675455 1.6001802552998756 7 0.2369543013304596 1.118389983149225 8 0.15377995981560727 0.8295075859471273 9 0.1094899170922922 0.6644269938481661 >10 0.49166555550943686 5.605527789500835 >50 0.024145717704045753 1.1284485471441015 >100 0.012818726224653625 1.6900104878767968 >500 0.0016044941321634292 0.8054405069759537 >1k 9.168537897860711E-4 1.2663490653214546 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 3899 0.15013743023557596 No Hit GTATCAACGCAGAGTACTTTTTTTT 3717 0.14312921984755989 No Hit CCCATGTACTCTGCGTTGATACCAC 3100 0.11937061649917559 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 3.850665048360502E-5 0.0 11 0.0 0.0 0.0 3.850665048360502E-5 0.0 12 0.0 0.0 0.0 3.850665048360502E-5 0.0 13 0.0 0.0 0.0 3.850665048360502E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCGC 125 1.4242687E-9 12.153183 10 TAGGACC 685 0.0 11.923536 4 TGTAGGA 1640 0.0 10.887075 2 AGGACGT 1385 0.0 10.765612 5 GTAGGAC 1485 0.0 10.744347 3 GGACGTG 1335 0.0 10.59644 6 AGGCGTG 220 0.0 10.357228 7 TAGGACG 1435 0.0 10.258337 4 GTGCGCC 195 1.4551915E-11 10.225034 11 CTGTAGG 1645 0.0 10.196733 1 TTAGGAC 1025 0.0 10.192155 3 GTCCTAC 1340 0.0 10.099453 1 CCTACAC 415 0.0 10.069358 3 TGGCGAG 1310 0.0 10.010508 18 CCTAGAC 190 1.0004442E-10 9.997089 3 GGCGAGG 645 0.0 9.862495 19 GCGTGCG 165 1.0322765E-8 9.7823925 9 GTCTTAA 420 0.0 9.757386 1 GACGTGA 760 0.0 9.74397 7 GTCCTAG 355 0.0 9.664702 1 >>END_MODULE