FastQCFastQC Report
Thu 26 May 2016
SRR1512980_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512980_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2596954
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48400.1863721883406483No Hit
GTACATGGGAAGCAGTGGTATCAAC37660.1450160457212565No Hit
CCCATGTACTCTGCGTTGATACCAC37390.14397636615819917No Hit
TATCAACGCAGAGTACTTTTTTTTT34860.1342341835858471No Hit
GGTATCAACGCAGAGTACTTTTTTT27620.10635536863571708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG1605.347829E-1010.6982111
GTAGGAC12500.010.6488143
AGGACGT11500.010.5910485
GGACGTG11350.010.3928646
TAGGACG11950.010.3514754
GTCCTAC14100.010.1839551
TATACCG750.002613251810.1497555
GGCGAGG5150.010.13546319
TGGCGAG12500.010.0978718
AAGACCG1054.0346593E-59.9685095
GTACTAA2151.0913936E-119.7306721
TGTAGGA14300.09.7073362
GGCGAGA8050.09.66732119
TAGGACC5450.09.6026924
GAGTAAG1401.044813E-69.5095211
TATGGCG13500.09.49175816
CTGTAGG14750.09.4128131
GACGTGG7100.09.369987
ACTGATC2550.09.3165888
TCCTACA17600.09.1297162
Produced by FastQC (version 0.11.5)