##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512980_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2596954 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.417053594326276 31.0 31.0 34.0 27.0 34.0 2 30.54989037156607 31.0 31.0 34.0 27.0 34.0 3 30.645270189614447 31.0 31.0 34.0 27.0 34.0 4 34.13395924610139 37.0 35.0 37.0 30.0 37.0 5 33.79372141362535 37.0 35.0 37.0 28.0 37.0 6 33.66102017979525 37.0 35.0 37.0 28.0 37.0 7 33.54028450253643 37.0 35.0 37.0 28.0 37.0 8 33.42441106003418 37.0 35.0 37.0 27.0 37.0 9 34.86755637566164 39.0 35.0 39.0 27.0 39.0 10 34.75928068036631 39.0 35.0 39.0 27.0 39.0 11 34.79996988779932 39.0 35.0 39.0 27.0 39.0 12 34.643294413378136 39.0 35.0 39.0 27.0 39.0 13 34.62776853190314 39.0 35.0 39.0 27.0 39.0 14 35.68168977964184 40.0 36.0 41.0 27.0 41.0 15 35.69111659274673 40.0 36.0 41.0 27.0 41.0 16 35.66321236340728 40.0 36.0 41.0 27.0 41.0 17 35.63819651792061 40.0 36.0 41.0 27.0 41.0 18 35.552478018478574 40.0 36.0 41.0 27.0 41.0 19 35.53038213229807 40.0 36.0 41.0 26.0 41.0 20 35.5046500631124 40.0 36.0 41.0 26.0 41.0 21 35.448185066042754 40.0 36.0 41.0 26.0 41.0 22 35.40829371640776 40.0 36.0 41.0 26.0 41.0 23 35.3024759006128 40.0 36.0 41.0 25.0 41.0 24 35.28411015366464 40.0 36.0 41.0 25.0 41.0 25 35.18681617002073 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5683654424487479 1101 2 0.5694279719299082 1101 3 0.5751810611856989 1101 4 0.33533714458835817 1101 5 0.6772220713325368 1101 6 0.7572074957096007 1101 7 0.7509074314416253 1101 8 0.7723975303827118 1101 9 0.47199565873124527 1101 10 0.8008343642316831 1101 11 0.9417347637560312 1101 12 0.8426982383969133 1101 13 0.9566692444834359 1101 14 0.9730584961308537 1101 15 0.8423699850090003 1101 16 0.9762566904151129 1101 17 0.9111690343377603 1101 18 0.4649669177507789 1101 19 0.9132945172328419 1101 20 0.8193244441735672 1101 21 1.019587248358377 1101 22 0.8442093622918563 1101 23 0.7580131642675454 1101 24 0.7537168066234443 1101 25 0.8261268585495856 1102 1 0.5164874473325298 1102 2 0.5048827423863465 1102 3 0.4085041956144053 1102 4 0.35890908681708567 1102 5 0.5339041402940197 1102 6 0.43821713529850115 1102 7 0.49673110992018366 1102 8 0.5307499460614835 1102 9 0.6275648128263001 1102 10 0.60530570693372 1102 11 0.5733511732089269 1102 12 0.5723398930620078 1102 13 0.605353748189799 1102 14 0.8296034320499075 1102 15 0.7787501274168918 1102 16 0.8364458833439556 1102 17 0.8141643291997909 1102 18 0.781398262557552 1102 19 0.8229559980488332 1102 20 0.8124138742756628 1102 21 0.7625879739747106 1102 22 0.8146032413900102 1102 23 0.8404882630247741 1102 24 0.7110601803561352 1102 25 0.7853299408274665 1103 1 0.5314251972416528 1103 2 0.559083487569918 1103 3 0.5930647342064326 1103 4 0.6447911145645691 1103 5 0.7007351411421965 1103 6 0.758491580954626 1103 7 0.7296736762596936 1103 8 0.712957173736271 1103 9 0.7999199412644415 1103 10 0.863655322225128 1103 11 0.9136524272983451 1103 12 0.9873972964304798 1103 13 0.9450499192791 1103 14 1.0415964194484246 1103 15 0.725764470686002 1103 16 0.9393501302844669 1103 17 1.0183256947638242 1103 18 1.222219403354842 1103 19 1.1728814039618385 1103 20 1.1792482829755286 1103 21 0.8469334392600629 1103 22 1.0942954745173594 1103 23 1.1253937519378994 1103 24 1.1803868645921725 1103 25 1.1808054497333202 1104 1 0.45772450812718546 1104 2 0.49797358000203573 1104 3 0.38638928167417674 1104 4 0.4173605590090119 1104 5 0.4287558627179422 1104 6 0.4286966647212367 1104 7 0.4907634674621377 1104 8 0.41906653111296066 1104 9 0.5622919358450602 1104 10 0.6612687819648855 1104 11 0.6445135108998983 1104 12 0.5782570733279471 1104 13 0.6877959922894661 1104 14 0.8058433624486199 1104 15 0.6965574853210867 1104 16 0.7741371719064247 1104 17 0.5940517752017769 1104 18 0.6632866195249605 1104 19 0.7126030587993952 1104 20 0.7980255346329841 1104 21 0.5156568753415982 1104 22 0.6799464882446316 1104 23 0.5898548396658825 1104 24 0.7438105840221709 1104 25 0.7382054352908014 1105 1 0.3954909322313007 1105 2 0.48524926020308 1105 3 0.3971586626210133 1105 4 0.3938568887662157 1105 5 0.298300468327092 1105 6 0.4694717239999875 1105 7 0.4966014948179449 1105 8 0.5747799825699502 1105 9 0.6640690017450268 1105 10 0.5647060076508126 1105 11 0.5847127980869757 1105 12 0.49225762330167555 1105 13 0.6421215674376981 1105 14 0.882035946708605 1105 15 0.7092670037809299 1105 16 0.7689406786250146 1105 17 0.5700521989359828 1105 18 0.852249322107582 1105 19 0.8249131735207769 1105 20 0.8657905299797335 1105 21 0.8514712474663497 1105 22 0.8958945397033844 1105 23 0.8398323930069722 1105 24 0.9448100169229292 1105 25 1.0189765630402974 1106 1 0.49890446422934787 1106 2 0.5402049603407555 1106 3 0.5040057115174044 1106 4 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1107 17 0.6372568541841162 1107 18 0.8499216980408306 1107 19 0.8692258656595584 1107 20 0.9013691421887842 1107 21 0.7944554371896686 1107 22 0.8556826380784344 1107 23 0.7746863675348621 1107 24 0.9336714574148104 1107 25 0.9025294835497419 1108 1 0.3176239790799684 1108 2 0.2969018707371376 1108 3 0.32824722774648407 1108 4 0.4004378015339327 1108 5 0.4504121437261759 1108 6 0.31524684119825963 1108 7 0.32466952343577304 1108 8 0.4518022272615525 1108 9 0.38457410331686503 1108 10 0.5758934636057091 1108 11 0.5424591825585878 1108 12 0.614831312672635 1108 13 0.5096032599952309 1108 14 0.6270119325528967 1108 15 0.5426999173452884 1108 16 0.5101096356369936 1108 17 0.6179703976062001 1108 18 0.6537035764814547 1108 19 0.5564927457853841 1108 20 0.7186712711630534 1108 21 0.7750376751404602 1108 22 0.7583550906118646 1108 23 0.7368970741970315 1108 24 0.719463784119668 1108 25 0.7378548683379691 1109 1 0.015363425925457364 1109 2 0.001995940161570786 1109 3 -8.542677601361959E-4 1109 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-2.000700833904304 2216 16 -1.8059978963135634 2216 17 -2.0307278230396903 2216 18 -1.7749987969883634 2216 19 -1.9501947972393623 2216 20 -2.331402993300749 2216 21 -2.0682250227733974 2216 22 -2.4406680604461712 2216 23 -1.5967748452306978 2216 24 -1.9854687231384176 2216 25 -1.7722793314764616 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 66626.0 3 3.0 4 1043.0 5 9190.0 6 10837.0 7 8412.0 8 6865.0 9 5762.0 10 5213.0 11 4688.0 12 4444.0 13 4162.0 14 4050.0 15 4100.0 16 4296.0 17 4524.0 18 4970.0 19 5876.0 20 7437.0 21 9590.0 22 12375.0 23 15341.0 24 17924.0 25 20989.0 26 23940.0 27 26623.0 28 30309.0 29 36288.0 30 45971.0 31 58749.0 32 74835.0 33 96270.0 34 125154.0 35 164304.0 36 225185.0 37 352178.0 38 1098431.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.72687573026849 19.10975839095839 13.771514981487018 27.391850897286098 2 13.994934065062377 20.292581231704528 39.02494999911434 26.687534704118747 3 17.212896339326768 24.117215784338114 29.78843676091298 28.881451115422145 4 11.904802256187606 17.007300563353592 36.67167647958315 34.41622070087565 5 13.407105016947169 37.58406149378604 35.36497677952961 13.643856709737184 6 32.879955265943735 36.63402431932594 17.34959194817817 13.136428466552161 7 28.341207399632136 31.417697870824224 21.723871613878643 18.517223115664994 8 25.836743186073928 36.157192284871044 19.9332285333395 18.07283599571553 9 26.19123562454592 15.07173955417698 19.628855765089654 39.10816905618745 10 14.903252938530336 28.860332452021574 34.007236804319106 22.22917780512898 11 34.56941636030297 22.381269063896035 23.904493851995813 19.144820723805182 12 23.17181936581019 24.675923322969144 30.999298356953776 21.15295895426689 13 28.731626488278035 21.522351655469244 25.552577191757635 24.193444664495082 14 21.401453377884796 21.361789893667204 27.1382180005214 30.0985387279266 15 23.595056502722898 29.66271652060599 23.145091044358853 23.59713593231226 16 22.93497248735281 27.821355729593506 26.467020253991336 22.776651529062352 17 22.387465875407493 28.063356196040008 27.032454567303247 22.51672336124925 18 22.41418348392105 27.249035016650392 28.30909194527705 22.027689554151507 19 23.608693710067957 26.92544067723512 27.20099872463911 22.264866888057817 20 23.853598558581872 26.81572205490651 27.271922112899023 22.0587572736126 21 23.370945389420132 26.378125732830537 26.991691410427077 23.259237467322254 22 23.49603322976308 27.00665280681292 26.704298005082116 22.793015958341886 23 22.777915973867845 26.93004958886449 27.089659655119036 23.202374782148624 24 23.214234830497578 26.710022588001177 27.32520483612725 22.750537745374004 25 23.135477829052352 26.65658289537728 27.083359004848834 23.124580270721534 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 122.0 1 122.0 2 311.5 3 501.0 4 501.0 5 501.0 6 1209.5 7 1918.0 8 1918.0 9 1918.0 10 2893.5 11 3869.0 12 3869.0 13 3869.0 14 6800.0 15 9731.0 16 9731.0 17 9731.0 18 18195.0 19 26659.0 20 26659.0 21 26659.0 22 42125.5 23 57592.0 24 57592.0 25 57592.0 26 82531.5 27 107471.0 28 107471.0 29 107471.0 30 136604.5 31 165738.0 32 165738.0 33 165738.0 34 194011.5 35 222285.0 36 222285.0 37 222285.0 38 247292.5 39 272300.0 40 272300.0 41 272300.0 42 292412.5 43 312525.0 44 312525.0 45 312525.0 46 330634.5 47 348744.0 48 348744.0 49 348744.0 50 342205.5 51 335667.0 52 335667.0 53 335667.0 54 305185.5 55 274704.0 56 274704.0 57 274704.0 58 238760.0 59 202816.0 60 202816.0 61 202816.0 62 166520.0 63 130224.0 64 130224.0 65 130224.0 66 100891.5 67 71559.0 68 71559.0 69 71559.0 70 52240.5 71 32922.0 72 32922.0 73 32922.0 74 22934.5 75 12947.0 76 12947.0 77 12947.0 78 8819.5 79 4692.0 80 4692.0 81 4692.0 82 3089.5 83 1487.0 84 1487.0 85 1487.0 86 939.5 87 392.0 88 392.0 89 392.0 90 236.0 91 80.0 92 80.0 93 80.0 94 44.0 95 8.0 96 8.0 97 8.0 98 4.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07770642067591493 2 0.0 3 0.0 4 0.027570761746261194 5 0.0704671703849972 6 0.07943921994767716 7 0.033847345775088813 8 0.059069201841850105 9 0.039892889901014805 10 0.10454555606298763 11 0.0036581317959424775 12 0.0073932768928521644 13 0.002965012087237587 14 0.004120211601745738 15 0.003388585242557242 16 0.012707194659589659 17 0.022564897183392545 18 6.931197087048904E-4 19 0.001617279320311411 20 0.0025799455824015364 21 1.1551995145081507E-4 22 1.925332524180251E-4 23 0.0 24 0.0 25 0.001617279320311411 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2596954.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.53578647796638 #Duplication Level Percentage of deduplicated Percentage of total 1 78.88076429130042 52.4839369010476 2 13.407668493757374 17.841795361359956 3 3.94663967412153 7.877783239884627 4 1.5539271483153068 4.135670597704897 5 0.7337035011230436 2.44087697444296 6 0.39100508351395635 1.5609498449090409 7 0.2462466579718336 1.1468950539008715 8 0.17710010095188697 0.9426795601728835 9 0.11294162950838209 0.6763194130898968 >10 0.5090231575139538 5.749231543886704 >50 0.024535745411583876 1.095434628509028 >100 0.013894026408053541 1.8359779301347876 >500 0.0014491421038364677 0.6873575386495238 >1k 0.0011013479988782509 1.525091412307279 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4840 0.1863721883406483 No Hit GTACATGGGAAGCAGTGGTATCAAC 3766 0.1450160457212565 No Hit CCCATGTACTCTGCGTTGATACCAC 3739 0.14397636615819917 No Hit TATCAACGCAGAGTACTTTTTTTTT 3486 0.1342341835858471 No Hit GGTATCAACGCAGAGTACTTTTTTT 2762 0.10635536863571708 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 160 5.347829E-10 10.698211 1 GTAGGAC 1250 0.0 10.648814 3 AGGACGT 1150 0.0 10.591048 5 GGACGTG 1135 0.0 10.392864 6 TAGGACG 1195 0.0 10.351475 4 GTCCTAC 1410 0.0 10.183955 1 TATACCG 75 0.0026132518 10.149755 5 GGCGAGG 515 0.0 10.135463 19 TGGCGAG 1250 0.0 10.09787 18 AAGACCG 105 4.0346593E-5 9.968509 5 GTACTAA 215 1.0913936E-11 9.730672 1 TGTAGGA 1430 0.0 9.707336 2 GGCGAGA 805 0.0 9.667321 19 TAGGACC 545 0.0 9.602692 4 GAGTAAG 140 1.044813E-6 9.509521 1 TATGGCG 1350 0.0 9.491758 16 CTGTAGG 1475 0.0 9.412813 1 GACGTGG 710 0.0 9.36998 7 ACTGATC 255 0.0 9.316588 8 TCCTACA 1760 0.0 9.129716 2 >>END_MODULE