FastQCFastQC Report
Thu 26 May 2016
SRR1512979_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512979_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827040
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT30100.1647473509063841No Hit
GTATCAACGCAGAGTACTTTTTTTT27510.15057141606095106No Hit
GTCCTAAAGTGTGTATTTCTCATTT27400.14996934933006392No Hit
CTGTAGGACGTGGAATATGGCAAGA26240.1436202819861634No Hit
CTTTAGGACGTGAAATATGGCGAGG23930.13097688063753393No Hit
GTCCTACAGTGTGCATTTCTCATTT20130.11017821175234258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5450.012.5474014
GGCGAGG7250.011.52412319
TTAGTAC1251.8388164E-811.39722253
CCTATAC1501.7644197E-1011.39722253
GGACGTG15850.011.3808766
GGTATCA9600.011.3100931
AGGACGT16100.011.2671335
AATGTCC4250.011.1703838
TGTAGGA18450.010.7074192
GACGTGG8300.010.6379077
GGTCTAT1451.3871613E-810.509561
GACGTGA8800.010.4650287
ATTAGGC1002.4054616E-510.4474543
TAGGACG17550.010.4447484
TGGCGAG15700.010.28947218
TCCAACG1303.826899E-710.23360318
GTCCTAC16750.010.1781951
GTCCTAA9600.010.1195571
TTAGGAC10900.010.1076293
CTGTAGG19150.010.0465081
Produced by FastQC (version 0.11.5)