FastQCFastQC Report
Thu 26 May 2016
SRR1512979_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512979_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827040
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33530.18352088624222787No Hit
GTCCTACAGTGGACATTTCTAAATT28960.1585077502408267No Hit
CTGTAGGACGTGGAATATGGCAAGA27110.1483820824940888No Hit
CTTTAGGACGTGAAATATGGCGAGG24990.13677861458971888No Hit
GTCCTAAAGTGTGTATTTCTCATTT24650.13491768105788599No Hit
TATCAACGCAGAGTACTTTTTTTTT21970.12024914615990892No Hit
GGTATCAACGCAGAGTACTTTTTTT20190.11050661178737192No Hit
GTCCTACAGTGTGCATTTCTCATTT19730.10798887818548034No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG508.6207634E-515.2194415
GGCGAAA802.020568E-613.050570519
TAGGACC6450.012.6828674
TCGGGAC604.0741035E-412.6734823
GGCGAGG7000.012.60990319
CTAGGAC2200.012.0974143
CGTGCGC1103.8038706E-812.09343810
TAGGACA5550.011.4831374
CGGTTTC752.0881502E-411.39177113
AATCCCG2000.011.38958919
GACGTGG8250.011.2915397
GTAGGAC18650.011.2124633
GGTATCA9300.011.1394831
TGTAGGA20000.011.1209812
AAGGCGT1158.695333E-710.7508026
AGGACGT17750.010.7179165
ACGTGGA8800.010.6923718
CTGTAGG19950.010.671531
GGACGTG17200.010.6714696
ATAGGAC2200.010.3692123
Produced by FastQC (version 0.11.5)