##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512979_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1827040 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.551510640161133 31.0 31.0 34.0 27.0 34.0 2 30.71676317978807 31.0 31.0 34.0 27.0 34.0 3 30.81550814432087 31.0 31.0 34.0 27.0 34.0 4 34.282590419476314 37.0 35.0 37.0 30.0 37.0 5 33.93883111480865 37.0 35.0 37.0 30.0 37.0 6 33.79415393204309 37.0 35.0 37.0 29.0 37.0 7 33.6762484674665 37.0 35.0 37.0 28.0 37.0 8 33.567395897188895 37.0 35.0 37.0 28.0 37.0 9 35.04830928715299 39.0 35.0 39.0 28.0 39.0 10 34.93842663543217 39.0 35.0 39.0 28.0 39.0 11 34.95460909449164 39.0 35.0 39.0 27.0 39.0 12 34.80618705665995 39.0 35.0 39.0 27.0 39.0 13 34.78371464226289 39.0 35.0 39.0 27.0 39.0 14 35.86139438654873 40.0 36.0 41.0 27.0 41.0 15 35.872788225764076 40.0 36.0 41.0 27.0 41.0 16 35.848304361152465 40.0 36.0 41.0 27.0 41.0 17 35.83193252473947 40.0 36.0 41.0 27.0 41.0 18 35.76211139329188 40.0 36.0 41.0 27.0 41.0 19 35.74687089499956 40.0 36.0 41.0 27.0 41.0 20 35.71867665732551 40.0 36.0 41.0 27.0 41.0 21 35.66800781592083 40.0 36.0 41.0 27.0 41.0 22 35.62615432612313 40.0 36.0 41.0 27.0 41.0 23 35.519554580085824 40.0 36.0 41.0 27.0 41.0 24 35.498679831859185 40.0 36.0 41.0 26.0 41.0 25 35.41062155179963 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3974176290439182 1101 2 0.3694084082415898 1101 3 0.40147725094389486 1101 4 0.203662128087025 1101 5 0.4273206071020752 1101 6 0.48418752284650424 1101 7 0.5312250507179286 1101 8 0.5269523234132691 1101 9 0.3548807530800886 1101 10 0.5857940885719088 1101 11 0.570032465989911 1101 12 0.5890573840153266 1101 13 0.6609504794684042 1101 14 0.8094076155257426 1101 15 0.5824613473862001 1101 16 0.7785172375644862 1101 17 0.6913995745078552 1101 18 0.25020737926377734 1101 19 0.576811478926011 1101 20 0.5366006025755468 1101 21 0.6779547456514265 1101 22 0.6087484624242876 1101 23 0.5277278358398689 1101 24 0.5160252920863755 1101 25 0.508074137324968 1102 1 0.6833942987761858 1102 2 0.6807780795348002 1102 3 0.626997917559617 1102 4 0.5538766577489724 1102 5 0.7427660480595222 1102 6 0.634928477144129 1102 7 0.5988482523052667 1102 8 0.5480568692831795 1102 9 0.7485772843206178 1102 10 0.8032842545151055 1102 11 0.7711535564814014 1102 12 0.8873615603031837 1102 13 0.9105591745861901 1102 14 1.0831553731042831 1102 15 0.9549774498025414 1102 16 0.925294643468284 1102 17 0.8484366764630167 1102 18 0.9176318957637761 1102 19 0.9811107826731842 1102 20 0.9464618062395189 1102 21 1.0076955732993227 1102 22 0.9646202800828902 1102 23 0.9429329095238188 1102 24 0.8660792384542262 1102 25 0.8805803535817702 1103 1 0.6452901048289483 1103 2 0.5396013327523868 1103 3 0.562800631443622 1103 4 0.6163734623357655 1103 5 0.6485428334477419 1103 6 0.7649844033965962 1103 7 0.6100945417131882 1103 8 0.7191499875369445 1103 9 0.8405723177355071 1103 10 0.968080713214718 1103 11 0.8831756139623153 1103 12 0.9288359370019705 1103 13 0.9530839944692246 1103 14 1.0628228936201438 1103 15 0.7780005976366979 1103 16 0.9134877872853551 1103 17 1.0279688157986868 1103 18 1.0371265248610797 1103 19 1.1084971694303505 1103 20 1.0553400988193786 1103 21 0.7682920852123445 1103 22 1.0609936817241632 1103 23 1.1691679041242224 1103 24 1.0754401036417036 1103 25 1.1559368051862577 1104 1 0.45288341523604103 1104 2 0.52596313355129 1104 3 0.4844219471904516 1104 4 0.4990436150945001 1104 5 0.536657540109303 1104 6 0.5349132583105245 1104 7 0.5955707585230812 1104 8 0.6666891031875792 1104 9 0.8180852233923943 1104 10 0.8404219678477602 1104 11 0.9568465306433467 1104 12 0.8090414758244009 1104 13 1.0141692271054268 1104 14 0.9367899909469273 1104 15 0.8845801034278509 1104 16 0.91355967828882 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2216 19 -2.0323924710032983 2216 20 -2.4651021077398028 2216 21 -2.2545072758395364 2216 22 -2.6161574800770495 2216 23 -1.8083253429138324 2216 24 -1.943274900698185 2216 25 -1.7816261961193547 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44338.0 3 0.0 4 730.0 5 6751.0 6 7706.0 7 5917.0 8 4710.0 9 4291.0 10 3726.0 11 3485.0 12 3089.0 13 2937.0 14 2866.0 15 2881.0 16 2936.0 17 3024.0 18 3364.0 19 4021.0 20 5112.0 21 6718.0 22 8170.0 23 10218.0 24 12112.0 25 13830.0 26 15940.0 27 17493.0 28 19926.0 29 24019.0 30 29852.0 31 38335.0 32 49172.0 33 63800.0 34 83877.0 35 110290.0 36 152588.0 37 238920.0 38 819896.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.79486864866819 19.342646264547575 13.342576149152974 27.51990893763126 2 13.593649170761182 20.728644247489243 40.30465721274391 25.373049369005663 3 17.029895349855504 24.863713985462827 30.281165163324285 27.825225501357387 4 11.253039131365478 17.351120201511996 38.35018348387789 33.045657183244636 5 12.40712321024249 38.31146353993798 36.442097353168954 12.839315896650575 6 31.387786940293193 38.105857180888655 18.02828073012357 12.478075148694588 7 27.347532097785322 31.70171644446878 22.765802513071804 18.184948944674094 8 24.5692563497036 37.53335319555378 20.6296908065668 17.267699648175817 9 26.03361854375131 14.952301268060191 19.99524710144372 39.01883308674478 10 14.070063495350851 29.25180243187906 35.27851880342302 21.39961526934707 11 34.112852904793705 22.36231376369747 25.06568216400836 18.459151167500465 12 22.794443672002355 24.775702888848258 32.357410535696495 20.072442903452888 13 28.754208533052207 21.989355989759943 25.99177971069705 23.2646557664908 14 20.48893406527842 22.000336086064305 27.84938308501828 29.661346763638992 15 22.477758978650918 31.208752231217947 23.44342955919727 22.87005923093386 16 22.00273602004882 28.264214795638793 27.772026795259976 21.961022389052417 17 21.435254557608534 28.674651435128638 27.93364141684838 21.956452590414447 18 21.280770651742586 27.72452484229827 29.74195755394699 21.25274695201215 19 23.20144938245251 27.080736842393343 27.665142297282134 22.052671477872014 20 23.28911761228538 27.55852370505629 28.041349745368244 21.111008937290087 21 22.62560781506221 26.909048316508265 27.333177890309766 23.132165978119755 22 22.795037651105233 28.13378813139081 27.446391372264834 21.624782845239125 23 22.013475348104038 27.813567300113846 27.84613363692092 22.326823714861195 24 22.78090396537786 27.04737008898004 28.34460567070544 21.82712027493666 25 22.480128386877126 27.45446305319495 27.978114678994494 22.08729388093343 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 100.0 1 100.0 2 239.5 3 379.0 4 379.0 5 379.0 6 923.0 7 1467.0 8 1467.0 9 1467.0 10 2249.5 11 3032.0 12 3032.0 13 3032.0 14 5407.0 15 7782.0 16 7782.0 17 7782.0 18 14595.0 19 21408.0 20 21408.0 21 21408.0 22 34530.5 23 47653.0 24 47653.0 25 47653.0 26 68170.0 27 88687.0 28 88687.0 29 88687.0 30 114510.5 31 140334.0 32 140334.0 33 140334.0 34 160690.0 35 181046.0 36 181046.0 37 181046.0 38 195562.5 39 210079.0 40 210079.0 41 210079.0 42 220556.0 43 231033.0 44 231033.0 45 231033.0 46 234361.5 47 237690.0 48 237690.0 49 237690.0 50 228399.0 51 219108.0 52 219108.0 53 219108.0 54 194723.5 55 170339.0 56 170339.0 57 170339.0 58 146080.0 59 121821.0 60 121821.0 61 121821.0 62 98890.5 63 75960.0 64 75960.0 65 75960.0 66 58292.5 67 40625.0 68 40625.0 69 40625.0 70 29358.0 71 18091.0 72 18091.0 73 18091.0 74 12504.5 75 6918.0 76 6918.0 77 6918.0 78 4693.5 79 2469.0 80 2469.0 81 2469.0 82 1618.0 83 767.0 84 767.0 85 767.0 86 483.5 87 200.0 88 200.0 89 200.0 90 122.0 91 44.0 92 44.0 93 44.0 94 25.5 95 7.0 96 7.0 97 7.0 98 4.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08089587529556004 2 1.6420001751466854E-4 3 0.0 4 0.024575269288028723 5 0.06754094053770032 6 0.08133374200893248 7 0.03229267011121815 8 0.06173920658551537 9 0.04302040458884316 10 0.10218714423329539 11 0.0032840003502933707 12 0.012150801296085471 13 0.005309133899640949 14 0.006951134074787635 15 0.00673220071810141 16 0.016365268412295297 17 0.02621726946317541 18 8.210000875733427E-4 19 0.0030103336544355895 20 0.004816533847096943 21 2.1893335668622473E-4 22 1.0946667834311237E-4 23 0.0 24 5.473333917155618E-5 25 0.0033934670286364828 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1827040.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.78366090249921 #Duplication Level Percentage of deduplicated Percentage of total 1 84.80082349115922 63.41716028215541 2 10.82162479244086 16.185614377839514 3 2.3978640447384607 5.379631548360487 4 0.837906990762987 2.5064700906021105 5 0.35552549508091724 1.3293749033162232 6 0.19933391964213235 0.894415215172995 7 0.13080959993331398 0.6847694534943175 8 0.08158702533677448 0.4881101149463163 9 0.06194791613566978 0.41694227585157495 >10 0.280561410374591 3.616474770851822 >50 0.01690447409136948 0.8760707216113278 >100 0.01254414341013402 1.9278716808562808 >500 0.001686686530098635 0.903741271541467 >1k 8.800103635297217E-4 1.3733532934001698 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3353 0.18352088624222787 No Hit GTCCTACAGTGGACATTTCTAAATT 2896 0.1585077502408267 No Hit CTGTAGGACGTGGAATATGGCAAGA 2711 0.1483820824940888 No Hit CTTTAGGACGTGAAATATGGCGAGG 2499 0.13677861458971888 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2465 0.13491768105788599 No Hit TATCAACGCAGAGTACTTTTTTTTT 2197 0.12024914615990892 No Hit GGTATCAACGCAGAGTACTTTTTTT 2019 0.11050661178737192 No Hit GTCCTACAGTGTGCATTTCTCATTT 1973 0.10798887818548034 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.4733339171556176E-5 0.0 0.0 0.0 0.0 7 5.4733339171556176E-5 0.0 0.0 0.0 0.0 8 5.4733339171556176E-5 0.0 0.0 0.0 0.0 9 5.4733339171556176E-5 0.0 0.0 5.4733339171556176E-5 0.0 10 5.4733339171556176E-5 0.0 0.0 5.4733339171556176E-5 0.0 11 1.0946667834311235E-4 0.0 0.0 5.4733339171556176E-5 0.0 12 1.0946667834311235E-4 0.0 0.0 5.4733339171556176E-5 0.0 13 1.0946667834311235E-4 0.0 0.0 5.4733339171556176E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCACCG 50 8.6207634E-5 15.219441 5 GGCGAAA 80 2.020568E-6 13.0505705 19 TAGGACC 645 0.0 12.682867 4 TCGGGAC 60 4.0741035E-4 12.673482 3 GGCGAGG 700 0.0 12.609903 19 CTAGGAC 220 0.0 12.097414 3 CGTGCGC 110 3.8038706E-8 12.093438 10 TAGGACA 555 0.0 11.483137 4 CGGTTTC 75 2.0881502E-4 11.391771 13 AATCCCG 200 0.0 11.389589 19 GACGTGG 825 0.0 11.291539 7 GTAGGAC 1865 0.0 11.212463 3 GGTATCA 930 0.0 11.139483 1 TGTAGGA 2000 0.0 11.120981 2 AAGGCGT 115 8.695333E-7 10.750802 6 AGGACGT 1775 0.0 10.717916 5 ACGTGGA 880 0.0 10.692371 8 CTGTAGG 1995 0.0 10.67153 1 GGACGTG 1720 0.0 10.671469 6 ATAGGAC 220 0.0 10.369212 3 >>END_MODULE