FastQCFastQC Report
Thu 26 May 2016
SRR1512978_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512978_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2357249
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29540.1253155691231601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9500.012.3347421
GTATTAG2550.012.3288421
TCCAACG1052.712477E-711.76507318
GACGTGA4550.011.2679527
TAATACT2900.011.1350194
TAGGACC4650.011.0293744
TAGGACG9750.010.6177384
GGACGTG9550.010.4387526
AGGACGT9750.010.4226955
TGTAGGA12250.010.3893412
ACGTGAA5650.010.2511288
GTAGGAC11150.010.2215423
CGTGAAA5700.010.1614219
AACACCG856.6127954E-410.0559815
GTCCTAA5700.010.0282461
GTCCTAC10600.09.796461
TTAGGAC6500.09.7897483
GTATCAA18300.09.7871291
CTGTAGG12700.09.6018491
ATTATAC2600.09.4975173
Produced by FastQC (version 0.11.5)