##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512978_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2357249
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.71365180343697	31.0	30.0	33.0	22.0	34.0
2	28.880077581961007	31.0	30.0	34.0	22.0	34.0
3	29.180775132368282	31.0	30.0	34.0	23.0	34.0
4	32.635401690699624	35.0	33.0	37.0	25.0	37.0
5	32.82450093307919	35.0	35.0	37.0	26.0	37.0
6	32.82993438537889	37.0	35.0	37.0	26.0	37.0
7	32.60875961767297	36.0	33.0	37.0	25.0	37.0
8	32.68063704767719	37.0	35.0	37.0	25.0	37.0
9	34.00408484636116	39.0	34.0	39.0	25.0	39.0
10	33.86669630573605	38.0	34.0	39.0	23.0	39.0
11	33.95529577062075	39.0	35.0	39.0	24.0	39.0
12	33.837220845146184	38.0	34.0	39.0	23.0	39.0
13	33.84939011534208	39.0	34.0	39.0	23.0	39.0
14	34.86281296545253	39.0	35.0	41.0	22.0	41.0
15	34.78127554619813	39.0	34.0	41.0	22.0	41.0
16	34.68520508440135	39.0	34.0	41.0	19.0	41.0
17	34.61863426392375	39.0	34.0	41.0	18.0	41.0
18	34.30836708383374	39.0	34.0	41.0	17.0	41.0
19	34.53780190382942	39.0	34.0	41.0	18.0	41.0
20	34.597787505689894	39.0	34.0	41.0	18.0	41.0
21	34.551924722420075	39.0	34.0	41.0	18.0	41.0
22	34.46934413801851	39.0	34.0	41.0	18.0	41.0
23	34.204643633320025	39.0	34.0	41.0	11.0	41.0
24	34.193499286668484	39.0	34.0	41.0	10.0	41.0
25	34.11558685569492	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.3290221970591922
1101	2	0.6655146367882097
1101	3	0.7023791074735115
1101	4	0.6618783080367692
1101	5	0.6235722332662519
1101	6	0.6874052164143052
1101	7	0.6509391155676099
1101	8	0.5576211407951703
1101	9	0.49493878458541474
1101	10	0.45562462555202643
1101	11	0.7368105042261561
1101	12	0.8852478731808517
1101	13	0.7892326953144249
1101	14	0.727382680587084
1101	15	0.484757543944518
1101	16	0.9170795799194167
1101	17	0.8800668120485042
1101	18	1.0940015131044447
1101	19	0.5886332144614883
1101	20	0.7945466815512958
1101	21	0.674260769653749
1101	22	0.7449743263078048
1101	23	0.8374432446306912
1101	24	0.5319276743139412
1101	25	0.703969097045487
1102	1	0.7185811760049177
1102	2	0.8291208248769166
1102	3	0.9398871621265528
1102	4	1.0735905478610874
1102	5	1.023005215908995
1102	6	0.839804011068054
1102	7	1.0875813847248423
1102	8	1.0906676164264937
1102	9	1.045289789934536
1102	10	1.2188021890090894
1102	11	1.2297173482450106
1102	12	1.3045769253039978
1102	13	1.1823310380011023
1102	14	1.2960446601318552
1102	15	1.4297086645705477
1102	16	1.3302949520878258
1102	17	1.2820794743785626
1102	18	1.6047060242389364
1102	19	1.6573636349502578
1102	20	1.581149253450171
1102	21	1.3761422157368628
1102	22	1.6413526219167807
1102	23	1.8059165960991166
1102	24	1.7249892281046684
1102	25	1.567128228283643
1103	1	0.3055339685116536
1103	2	0.5025657365849163
1103	3	0.5196819031074504
1103	4	0.6196673921523228
1103	5	0.14151152842654824
1103	6	0.6217695207743077
1103	7	0.523695852899138
1103	8	0.4529508257095287
1103	9	0.8271182422714176
1103	10	0.7300764211830852
1103	11	0.7585682217999263
1103	12	1.017216974299778
1103	13	0.90358934951972
1103	14	1.1470060685226784
1103	15	1.1298889797430363
1103	16	1.2258306278832265
1103	17	1.096759032167867
1103	18	1.333187199661964
1103	19	1.3444078485873163
1103	20	1.212830763043442
1103	21	1.0670048595764925
1103	22	1.1979345716693501
1103	23	1.569448781539819
1103	24	1.4646670392301004
1103	25	1.3482977052209932
1104	1	0.4501314259059299
1104	2	0.5173427255591037
1104	3	0.5326914474403956
1104	4	0.8045441677846625
1104	5	0.6494798660878303
1104	6	0.6212749615848026
1104	7	0.7534309054520989
1104	8	0.7709051266819884
1104	9	0.9178586435369667
1104	10	0.9240425353062491
1104	11	0.9801426081179159
1104	12	0.8068240217462446
1104	13	0.8608800971305612
1104	14	1.0160889975263956
1104	15	1.177382927437577
1104	16	1.0489498852267687
1104	17	1.0159225633292692
1104	18	1.2214508924672955
1104	19	1.1574095239340494
1104	20	1.142292555158896
1104	21	1.1319688358851252
1104	22	1.123305370169227
1104	23	1.430293673270917
1104	24	1.204312398528451
1104	25	1.073261250713621
1105	1	0.44565191468261034
1105	2	0.5716486193277497
1105	3	0.6794854539933262
1105	4	0.8239051751226469
1105	5	0.4970918756692342
1105	6	0.35837948469046665
1105	7	0.5995647829917061
1105	8	0.6357156457931694
1105	9	0.5460654099224129
1105	10	0.8166767460561672
1105	11	0.6748651722467542
1105	12	0.6915711620724352
1105	13	0.6419782548274284
1105	14	0.7844000518568706
1105	15	1.0282410620032323
1105	16	1.0165086705236561
1105	17	0.8325175106833385
1105	18	0.8852343728607153
1105	19	0.8720045680045132
1105	20	0.9882842408643455
1105	21	0.9359061155211208
1105	22	0.7038373694217341
1105	23	0.900214118633329
1105	24	1.0112412559828314
1105	25	0.9941649709867519
1106	1	0.18020329778445543
1106	2	0.28102635751097793
1106	3	0.2694425110886627
1106	4	0.23784245499911805
1106	5	0.3644336393074994
1106	6	0.2835723727901538
1106	7	0.1187195086585291
1106	8	0.3297988634254949
1106	9	0.37123255900992547
1106	10	0.4214531364119267
1106	11	0.2682077501633131
1106	12	0.4016270822679857
1106	13	0.37431616911091226
1106	14	0.5668019256454571
1106	15	0.6161637886312121
1106	16	0.5283290711411084
1106	17	0.5378791150616422
1106	18	0.7346492953655925
1106	19	0.4371532988240219
1106	20	0.49708106204604263
1106	21	0.35840273071886486
1106	22	0.5642581268212652
1106	23	0.7781330210563127
1106	24	0.5788880793705289
1106	25	0.6506105959223518
1107	1	0.3593058924569945
1107	2	0.45265845189306475
1107	3	0.5868709897265383
1107	4	0.6083635475479383
1107	5	0.504902422363557
1107	6	0.40011846040377463
1107	7	0.6320002329677621
1107	8	0.6349226889737238
1107	9	0.7221023909445421
1107	10	0.4783726287558352
1107	11	0.6125284590761666
1107	12	0.67301032978677
1107	13	0.6674504862749799
1107	14	0.8381965647892002
1107	15	1.039020482696742
1107	16	1.0188097764878847
1107	17	1.0519767816181655
1107	18	1.2953679300121266
1107	19	1.2813418365851348
1107	20	1.0478858697363123
1107	21	1.0468303256213574
1107	22	0.9572453434369095
1107	23	0.9562115723246407
1107	24	1.022428568106612
1107	25	1.051788744663014
1108	1	0.5433294660127075
1108	2	0.5723621407566348
1108	3	0.4088496018445724
1108	4	0.624046904497952
1108	5	0.575871847278151
1108	6	0.6044333226374619
1108	7	0.6680288595897181
1108	8	0.6393332700832985
1108	9	0.8647054296014716
1108	10	0.7705128986468921
1108	11	0.5610618246867958
1108	12	0.72605416917105
1108	13	0.8075930072291371
1108	14	1.00510607636555
1108	15	0.9656346431079399
1108	16	0.8476511398418793
1108	17	0.899591774700113
1108	18	1.1880173324995624
1108	19	1.145902131477186
1108	20	1.0667150108472185
1108	21	1.054004650145913
1108	22	0.8997144730559867
1108	23	1.1454406249512417
1108	24	1.2128556370708168
1108	25	1.26366491114986
1109	1	0.1195944320042841
1109	2	0.023343703230708
1109	3	0.2688859384509108
1109	4	0.36613488785016557
1109	5	0.2158283144659876
1109	6	0.11669946711356971
1109	7	0.31123293213424574
1109	8	0.2724685807646736
1109	9	0.40840925747500023
1109	10	0.4875225280168962
1109	11	0.15284350365473642
1109	12	0.35244790491621814
1109	13	0.45375091061033856
1109	14	0.28648407358527805
1109	15	0.5392086514284458
1109	16	0.5571106793863052
1109	17	0.34450270260428795
1109	18	-1.1300168672569555
1109	19	0.17453583444151377
1109	20	0.442473363948956
1109	21	0.5499396404691268
1109	22	0.7038777744381619
1109	23	0.23606413808812476
1109	24	0.6762989114968505
1109	25	0.8021514898026965
1110	1	0.19548003596885266
1110	2	0.18001992102821518
1110	3	0.31064975926580374
1110	4	0.05795768094978371
1110	5	0.3971706392316321
1110	6	0.18321351962552512
1110	7	0.40532035160676827
1110	8	0.20160525144213182
1110	9	0.4749360561580147
1110	10	0.6980719956234935
1110	11	0.560883278506374
1110	12	-0.09720556918016854
1110	13	0.2505684466225162
1110	14	0.34049526528995244
1110	15	0.43802274767393357
1110	16	0.5377571765119029
1110	17	0.08809562728971798
1110	18	0.5666664657364819
1110	19	0.4705067414477142
1110	20	0.44129306850970806
1110	21	0.3368927730814235
1110	22	0.3627483163940255
1110	23	0.2971675826133904
1110	24	0.5663535557483854
1110	25	0.4757254481855995
1111	1	0.1106925089580919
1111	2	0.4169696807185268
1111	3	0.35494244794422514
1111	4	0.20308047995936107
1111	5	0.18102004204716593
1111	6	0.3768627363571966
1111	7	0.4027519214238211
1111	8	0.22168996396356988
1111	9	0.4975805892731202
1111	10	0.45374863345922023
1111	11	0.23446821225047643
1111	12	0.43695158997056893
1111	13	0.48702337381750027
1111	14	0.3251706341597824
1111	15	0.5794415262679848
1111	16	0.5286399133223014
1111	17	0.3879279300017089
1111	18	0.6050770538385564
1111	19	0.2353451496718293
1111	20	0.3180164456167205
1111	21	0.4662662985068593
1111	22	0.3153319519871829
1111	23	0.6386907269404603
1111	24	0.7103305497584458
1111	25	0.20992437114057338
1112	1	-0.1936771372355004
1112	2	0.11019790689885411
1112	3	0.05047974165058022
1112	4	0.25356856363194424
1112	5	0.10201775519636413
1112	6	0.14070320088967492
1112	7	-0.30890397013386917
1112	8	0.03872181611851033
1112	9	0.34483696418423193
1112	10	0.37977335757832265
1112	11	0.25984750388435174
1112	12	0.37354249762361746
1112	13	0.0899358533314043
1112	14	0.16815794135065687
1112	15	0.26480952121493573
1112	16	0.3335720397728039
1112	17	0.3963835987448263
1112	18	0.4349887916671378
1112	19	0.19490431993179413
1112	20	0.14777685963044007
1112	21	0.1728845856919463
1112	22	0.17588216449524907
1112	23	0.3917781520859336
1112	24	0.08525534763411713
1112	25	0.37037322538314754
1113	1	-0.17252290884211163
1113	2	-0.07124624527850543
1113	3	-0.20706291457248582
1113	4	0.1757020445042201
1113	5	-0.1426588393729773
1113	6	0.012332556895366054
1113	7	8.887410864630851E-4
1113	8	0.19938725577969763
1113	9	0.010277485825049837
1113	10	-0.15897239501228455
1113	11	-0.1327218278049429
1113	12	0.1605532908548355
1113	13	0.08907776692646507
1113	14	0.12026909060850244
1113	15	-0.18624986808740118
1113	16	-0.2322423342034341
1113	17	-0.33110014802166177
1113	18	-0.2533504544099827
1113	19	-0.18658066072379853
1113	20	-0.2521555804956819
1113	21	-0.13531585790007483
1113	22	0.04045423127670489
1113	23	-0.46460369614914754
1113	24	0.2171644597416318
1113	25	0.1091562212326096
1114	1	-1.4526175577146496
1114	2	-1.3008861708227855
1114	3	-1.2796412748048631
1114	4	-1.4589656161439777
1114	5	-1.5090118677072546
1114	6	-1.9079071018066749
1114	7	-2.1194193119057587
1114	8	-1.6530587400191017
1114	9	-1.8168298188941563
1114	10	-2.013438286698488
1114	11	-2.5449022238415573
1114	12	-2.104839198314135
1114	13	-2.036725924042816
1114	14	-2.3970332713749585
1114	15	-2.252108982816793
1114	16	-2.9038800697369958
1114	17	-4.807549694749827
1114	18	-3.941764821623458
1114	19	-2.892983945507634
1114	20	-2.0178453351703567
1114	21	-2.937550690682425
1114	22	-3.0973419746400417
1114	23	-3.118310864079774
1114	24	-2.554072025418897
1114	25	-2.5413640514620432
1115	1	-0.7230988326950971
1115	2	-0.3609678065023374
1115	3	-0.5240328581734772
1115	4	-0.61287715636265
1115	5	-0.4342053727314479
1115	6	-0.5280933952406599
1115	7	-1.6035681648682498
1115	8	-1.0486010085485837
1115	9	-0.7856863333639836
1115	10	-0.5766147470156042
1115	11	-1.301077222705402
1115	12	-1.0439969371995517
1115	13	-0.8378848612720802
1115	14	-0.6840883473537787
1115	15	-1.846248638908854
1115	16	-0.8020432669470807
1115	17	-0.895848321557807
1115	18	-0.8911404749122056
1115	19	-1.052194748780714
1115	20	-0.7235795659460251
1115	21	-1.4968101094019417
1115	22	-1.5075786056912577
1115	23	-0.7881273691733384
1115	24	-0.7928288812134028
1115	25	-1.2161607994295238
1116	1	-0.8595258113377184
1116	2	-1.6762049965506058
1116	3	-1.3602703772234328
1116	4	-1.006320474161889
1116	5	-1.6197243508345593
1116	6	-1.2599540258398356
1116	7	-0.9117835008848587
1116	8	-1.6125872673708095
1116	9	-1.6704697216779891
1116	10	-2.4746057883374597
1116	11	-1.2048598282222969
1116	12	-1.1410001225913717
1116	13	-1.7500018610792196
1116	14	-1.5829400243169829
1116	15	-1.1925996509356906
1116	16	-1.0653037051874108
1116	17	-1.4617011292279614
1116	18	-1.2769723197545062
1116	19	-1.5738031321470984
1116	20	-1.9210429755380005
1116	21	-2.357031977551678
1116	22	-1.433599550871513
1116	23	-1.3938725769044993
1116	24	-2.00950863651682
1116	25	-1.6389100056026535
1201	1	0.35974921861436826
1201	2	0.41981422930626877
1201	3	0.6160711238334287
1201	4	0.7644886317751656
1201	5	0.7943917764433266
1201	6	0.9101984028501775
1201	7	0.6590386048312951
1201	8	0.17333690780655076
1201	9	0.5438379953394232
1201	10	0.9139302905240996
1201	11	0.4181734253068399
1201	12	-0.16777998153749252
1201	13	0.3172741152819043
1201	14	0.8916399124708079
1201	15	0.4947591158060405
1201	16	0.9352145589527154
1201	17	1.0788999441330276
1201	18	1.4992784811527784
1201	19	0.9002868793909897
1201	20	0.8587297763912005
1201	21	0.7532079547238695
1201	22	1.10400395869204
1201	23	1.28322539197422
1201	24	1.0618632907742196
1201	25	1.0412788648594145
1202	1	0.33974820412406714
1202	2	0.44692727170632907
1202	3	0.2534492300054758
1202	4	0.5730062923605246
1202	5	0.5402010860826749
1202	6	0.5447408959037361
1202	7	0.4806440920966182
1202	8	0.6836165126062212
1202	9	0.9529826109321391
1202	10	1.086988809706419
1202	11	0.721429150131982
1202	12	0.8499720206718422
1202	13	0.8383402318693882
1202	14	1.0046757747030242
1202	15	0.6468307613656705
1202	16	0.8228004665551936
1202	17	1.031053876128361
1202	18	1.3482393612794752
1202	19	1.1028384352245908
1202	20	0.9632089765219618
1202	21	0.987399885017112
1202	22	1.1144038369532012
1202	23	1.2755393879951669
1202	24	1.3229283928713969
1202	25	1.0902686015991847
1203	1	0.36609738587919693
1203	2	0.5173473139097595
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2211	13	-0.09338502454725273
2211	14	0.0818721015657431
2211	15	0.10216339726787282
2211	16	0.041945725960822244
2211	17	0.14664013341402438
2211	18	0.05890972543816275
2211	19	-0.16172841022798679
2211	20	-0.2838260002026587
2211	21	-0.13938135962069964
2211	22	-0.2758768514002483
2211	23	0.19255526839125992
2211	24	-0.041908015247585695
2211	25	0.1535776355463625
2212	1	0.02846324569625125
2212	2	0.02982220745840536
2212	3	-0.03643739130971824
2212	4	-0.12409121330841089
2212	5	-0.12374449214861727
2212	6	-0.16650626962914572
2212	7	-0.2462493319396586
2212	8	-0.14321644279816326
2212	9	-0.06186243819862369
2212	10	0.044169459744210826
2212	11	-0.20390720663294815
2212	12	-0.04166819096836605
2212	13	-0.17498554288496138
2212	14	-0.2323083522811018
2212	15	-0.17655548202057503
2212	16	-0.1382048244500993
2212	17	-0.06194687972953972
2212	18	-2.6522595049336744
2212	19	-1.0617798565365533
2212	20	-0.5348484686783408
2212	21	-0.6621692759806237
2212	22	-0.3358455961533693
2212	23	-0.4335006724734711
2212	24	-0.6574495360779622
2212	25	-0.3014169615149527
2213	1	-0.2797141679267092
2213	2	-0.1087227731657805
2213	3	-0.4593196795385417
2213	4	-0.48377409279326855
2213	5	-0.564013615586056
2213	6	-0.5120780082701941
2213	7	-0.310552673470724
2213	8	-0.8505771273865719
2213	9	-0.6231787757525851
2213	10	-0.7264881143787107
2213	11	-0.728498417362772
2213	12	-0.7735655763311016
2213	13	-0.586528273734622
2213	14	-0.6968535905887165
2213	15	-0.8417449996853392
2213	16	-1.4897655081890022
2213	17	-0.9251872063361972
2213	18	-0.8243816978479472
2213	19	-0.6479062233559887
2213	20	-1.375090761890931
2213	21	-1.0509426320807123
2213	22	-1.0670211847547009
2213	23	-1.290103102343224
2213	24	-1.031041232441936
2213	25	-1.233073087230487
2214	1	-1.7015505777859765
2214	2	-3.9236621496672086
2214	3	-2.1611060100559776
2214	4	-1.7516150858025306
2214	5	-1.6529919519713125
2214	6	-1.6842351104806852
2214	7	-1.8771263734146046
2214	8	-1.6360705121044887
2214	9	-1.8333006051029272
2214	10	-1.975111224413208
2214	11	-1.5716293756302662
2214	12	-2.220506868531899
2214	13	-2.0413336847360775
2214	14	-3.063826566443126
2214	15	-3.313461780700745
2214	16	-2.6189283383133173
2214	17	-3.031766409821099
2214	18	-2.4694445521838944
2214	19	-4.7011461203431715
2214	20	-3.0934779498779577
2214	21	-2.7991166552912787
2214	22	-2.8972879760712402
2214	23	-3.01419052720696
2214	24	-3.2310961616939835
2214	25	-2.9866979515281606
2215	1	-0.7976755027888665
2215	2	-2.5018558393034382
2215	3	-1.3643393155803558
2215	4	-0.9597478595795828
2215	5	-0.9804883069929922
2215	6	-1.4514180369431458
2215	7	-1.8493175238346673
2215	8	-1.7134020713636815
2215	9	-1.540430910901172
2215	10	-2.9302327157752828
2215	11	-2.0405998849457383
2215	12	-2.2392458564231283
2215	13	-1.5333137034477602
2215	14	-2.8251699289415626
2215	15	-2.72298035861305
2215	16	-2.285414484229591
2215	17	-2.6809531166128693
2215	18	-1.5485705805743564
2215	19	-2.125082744420446
2215	20	-2.3043956295662866
2215	21	-2.1444429303213184
2215	22	-1.8112557229601478
2215	23	-2.3450743770639306
2215	24	-2.1028531599732077
2215	25	-1.9830772730635289
2216	1	-1.6491243157576747
2216	2	-1.6555565179260299
2216	3	-2.266807338641403
2216	4	-3.1241410714393645
2216	5	-2.109303764401208
2216	6	-2.1631104776553975
2216	7	-1.9360003132383277
2216	8	-2.4431786714868373
2216	9	-2.8757009401581968
2216	10	-2.221541592745826
2216	11	-2.824472628055023
2216	12	-3.4279720113098833
2216	13	-3.9702718343163106
2216	14	-3.1504916126402627
2216	15	-3.041118437731445
2216	16	-2.200710452375489
2216	17	-2.416709539917669
2216	18	-2.326560811216705
2216	19	-2.602895741941417
2216	20	-2.5449885549178006
2216	21	-3.0003560717345223
2216	22	-3.448871259493142
2216	23	-3.4523422112464743
2216	24	-3.5052586489356194
2216	25	-3.5521674019284433
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	116745.0
3	1.0
4	898.0
5	5635.0
6	6861.0
7	6169.0
8	5476.0
9	5142.0
10	4834.0
11	4621.0
12	4483.0
13	4264.0
14	4346.0
15	4394.0
16	4671.0
17	4906.0
18	5647.0
19	6745.0
20	8339.0
21	10748.0
22	13481.0
23	16285.0
24	18961.0
25	21588.0
26	24253.0
27	26830.0
28	30190.0
29	36117.0
30	44049.0
31	55518.0
32	69984.0
33	89598.0
34	116667.0
35	153909.0
36	211477.0
37	337660.0
38	875757.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.788269145696205	18.349859771163228	12.122013351437266	25.739857731703296
2	15.370950129883578	20.107446248229778	40.31411412864267	24.207489493243976
3	18.675671271384097	24.49868953974327	29.55936622611211	27.266272962760524
4	12.462103722916682	16.61036685972691	38.26149025306056	32.66603916429585
5	13.362582629686592	37.69543385041608	35.83093974611	13.111043773787333
6	32.04820043915013	37.12719442470272	18.036086684416535	12.788518451730614
7	28.101329413941112	31.614992693569494	22.345499968601867	17.93817792388753
8	25.31854350047355	36.18920643757447	20.623983732937745	17.868266329014233
9	26.491651638909513	15.15684735334677	19.745878858597646	38.60562214914607
10	14.638135911454523	28.772613190594694	34.76818761390132	21.821063284049465
11	34.73255704916405	22.32912406445566	24.31220863354836	18.626110252831932
12	22.88536673953482	24.653211516789096	31.649869651997992	20.811552091678095
13	28.79985845045681	21.71987786684499	25.68606551673221	23.79419816596599
14	21.32765616526364	21.30194086839624	27.587421400728346	29.78298156561177
15	23.325590415189584	29.725719077198054	23.3467228954723	23.601967612140058
16	22.925827468934777	27.83401612824522	26.52012211809983	22.720034284720175
17	22.243080119218156	27.928092305812225	27.252865037713946	22.575962537255673
18	22.19995090874037	27.47050914770609	28.321877290536175	22.00766265301737
19	23.55929233055409	27.019604631515165	27.169479219752553	22.251623818178192
20	23.598835830255936	27.09749773093829	27.35637656198219	21.94728987682358
21	23.575202748347632	26.643627981817996	26.86415606106383	22.91701320877054
22	23.372177490529403	27.225081128053336	26.95157096498255	22.45117041643471
23	23.049923155324688	27.04610086468293	26.96395216705379	22.94002381293859
24	23.09176071042302	26.754449796821362	27.452135168425905	22.701654324329716
25	23.146404107772593	26.867662153440637	27.182720945320643	22.803212793466127
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	232.0
1	232.0
2	418.5
3	605.0
4	605.0
5	605.0
6	1110.0
7	1615.0
8	1615.0
9	1615.0
10	2623.0
11	3631.0
12	3631.0
13	3631.0
14	6649.0
15	9667.0
16	9667.0
17	9667.0
18	17683.0
19	25699.0
20	25699.0
21	25699.0
22	40156.0
23	54613.0
24	54613.0
25	54613.0
26	77429.5
27	100246.0
28	100246.0
29	100246.0
30	128761.5
31	157277.0
32	157277.0
33	157277.0
34	183213.0
35	209149.0
36	209149.0
37	209149.0
38	232601.0
39	256053.0
40	256053.0
41	256053.0
42	273658.0
43	291263.0
44	291263.0
45	291263.0
46	299032.0
47	306801.0
48	306801.0
49	306801.0
50	300714.0
51	294627.0
52	294627.0
53	294627.0
54	269579.0
55	244531.0
56	244531.0
57	244531.0
58	211446.0
59	178361.0
60	178361.0
61	178361.0
62	146300.0
63	114239.0
64	114239.0
65	114239.0
66	88541.0
67	62843.0
68	62843.0
69	62843.0
70	45681.5
71	28520.0
72	28520.0
73	28520.0
74	19911.5
75	11303.0
76	11303.0
77	11303.0
78	7718.5
79	4134.0
80	4134.0
81	4134.0
82	2725.0
83	1316.0
84	1316.0
85	1316.0
86	849.0
87	382.0
88	382.0
89	382.0
90	241.5
91	101.0
92	101.0
93	101.0
94	66.5
95	32.0
96	32.0
97	32.0
98	20.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.36432298836482696
2	0.03851947757746424
3	0.03499842401036123
4	0.03465904535329106
5	0.040428482523483945
6	0.017987068824718982
7	0.01785980182831767
8	0.024817064298256142
9	0.021168743734751822
10	0.02095663207408297
11	0.019768806774337375
12	0.02040514175634394
13	0.02023545242780886
14	0.02863507419029555
15	0.029144142175900806
16	0.02180507871675839
17	0.023798928327045634
18	0.10397713605987317
19	0.02596246726586797
20	0.02324743800930661
21	0.026853336240677164
22	0.02040514175634394
23	0.023205015677172838
24	0.01934458345299966
25	0.02316259334503907
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2357249.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.98030974383104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.14435576995501	63.091698588263434
2	10.838140244961334	16.25294225228563
3	2.714530840573844	6.106090897062265
4	0.9638722943306011	2.8908577272962215
5	0.4441717282503212	1.6652066881830918
6	0.2523367875285663	1.1352174293193111
7	0.15493261540279854	0.8131826844626578
8	0.09837598799243093	0.5901009640822299
9	0.06893365100049528	0.46517998534112553
>10	0.2935114348858993	3.704777844002756
>50	0.01587389016111862	0.8204016260362413
>100	0.009603572540906153	1.428160084833193
>500	9.074549444938699E-4	0.4659975302284164
>1k	4.5372747224636524E-4	0.5701856986034091
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2954	0.1253155691231601	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.242233213377119E-5	0.0
4	0.0	0.0	0.0	4.242233213377119E-5	0.0
5	0.0	1.6968932853508477E-4	0.0	4.242233213377119E-5	0.0
6	0.0	1.6968932853508477E-4	0.0	4.242233213377119E-5	0.0
7	0.0	2.1211166066885593E-4	0.0	4.242233213377119E-5	0.0
8	0.0	2.1211166066885593E-4	0.0	4.242233213377119E-5	0.0
9	0.0	2.1211166066885593E-4	0.0	4.242233213377119E-5	8.484466426754238E-5
10	0.0	2.1211166066885593E-4	0.0	8.484466426754238E-5	8.484466426754238E-5
11	0.0	2.1211166066885593E-4	0.0	8.484466426754238E-5	8.484466426754238E-5
12	0.0	2.1211166066885593E-4	0.0	1.2726699640131357E-4	8.484466426754238E-5
13	0.0	2.1211166066885593E-4	0.0	1.2726699640131357E-4	8.484466426754238E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	950	0.0	12.334742	1
GTATTAG	255	0.0	12.328842	1
TCCAACG	105	2.712477E-7	11.765073	18
GACGTGA	455	0.0	11.267952	7
TAATACT	290	0.0	11.135019	4
TAGGACC	465	0.0	11.029374	4
TAGGACG	975	0.0	10.617738	4
GGACGTG	955	0.0	10.438752	6
AGGACGT	975	0.0	10.422695	5
TGTAGGA	1225	0.0	10.389341	2
ACGTGAA	565	0.0	10.251128	8
GTAGGAC	1115	0.0	10.221542	3
CGTGAAA	570	0.0	10.161421	9
AACACCG	85	6.6127954E-4	10.055981	5
GTCCTAA	570	0.0	10.028246	1
GTCCTAC	1060	0.0	9.79646	1
TTAGGAC	650	0.0	9.789748	3
GTATCAA	1830	0.0	9.787129	1
CTGTAGG	1270	0.0	9.601849	1
ATTATAC	260	0.0	9.497517	3
>>END_MODULE