FastQCFastQC Report
Thu 26 May 2016
SRR1512978_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512978_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2357249
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37150.15759896387695996No Hit
TATCAACGCAGAGTACTTTTTTTTT24890.10558918468095649No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG604.0427144E-412.6853945
GGTATCA8650.012.3080341
CACGACT600.005812901511.099724
ATCGTGC600.005860922411.087468
TAGGACC4600.010.9618364
ATACCGT700.001476760510.8704216
GTACTAG1451.4308171E-810.4891131
GTATCAA18950.010.4839231
TGGCGAG8300.010.17627218
CGTACTG750.002616174310.1483155
ACGACTC750.002616174310.1483155
GACGTGG4800.010.0983667
ACACCGC951.6244776E-410.0122316
TCCAACG1054.1533327E-59.94214718
TGTACCG1151.0089843E-59.92775
GTCCTAC10650.09.8181541
TGTAGGA11250.09.8017022
GTAGGAC10550.09.6415253
GGACGTG8700.09.6209486
GGGCCGT900.00110157729.5116196
Produced by FastQC (version 0.11.5)