##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512978_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2357249 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.514645249610883 31.0 31.0 34.0 27.0 34.0 2 30.674208367465635 31.0 31.0 34.0 27.0 34.0 3 30.76634118839376 31.0 31.0 34.0 27.0 34.0 4 34.24469116330095 37.0 35.0 37.0 30.0 37.0 5 33.90222119088819 37.0 35.0 37.0 30.0 37.0 6 33.766089623964206 37.0 35.0 37.0 29.0 37.0 7 33.64611375378672 37.0 35.0 37.0 28.0 37.0 8 33.53250674833249 37.0 35.0 37.0 28.0 37.0 9 35.007178282820355 39.0 35.0 39.0 28.0 39.0 10 34.8932866235175 39.0 35.0 39.0 27.0 39.0 11 34.921169549759064 39.0 35.0 39.0 27.0 39.0 12 34.775770400157135 39.0 35.0 39.0 27.0 39.0 13 34.76116311853351 39.0 35.0 39.0 27.0 39.0 14 35.83791657139318 40.0 36.0 41.0 27.0 41.0 15 35.83282673998377 40.0 36.0 41.0 27.0 41.0 16 35.80727916312617 40.0 36.0 41.0 27.0 41.0 17 35.777901698123536 40.0 36.0 41.0 27.0 41.0 18 35.70124496818113 40.0 36.0 41.0 27.0 41.0 19 35.68789253914203 40.0 36.0 41.0 27.0 41.0 20 35.65508904659627 40.0 36.0 41.0 27.0 41.0 21 35.605905867390334 40.0 36.0 41.0 27.0 41.0 22 35.56438331292112 40.0 36.0 41.0 27.0 41.0 23 35.45896169645209 40.0 36.0 41.0 26.0 41.0 24 35.43943342430095 40.0 36.0 41.0 26.0 41.0 25 35.34415286632851 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4642169855484646 1101 2 0.5506674894962487 1101 3 0.5023206312258885 1101 4 0.3000462705172353 1101 5 0.5038761030181007 1101 6 0.5249555027750787 1101 7 0.560911315645491 1101 8 0.5198460764601265 1101 9 0.4350110004730965 1101 10 0.786875132673515 1101 11 0.7477076939294918 1101 12 0.8223679576481331 1101 13 0.8351191498976362 1101 14 0.8802842169735854 1101 15 0.85499970649483 1101 16 0.933265608302932 1101 17 0.9235474927844933 1101 18 0.39971741387833504 1101 19 0.7139001444197817 1101 20 0.6421243609231624 1101 21 0.8511092406091123 1101 22 0.7362971639895619 1101 23 0.6175197728107449 1101 24 0.6112424988316931 1101 25 0.7491975979174015 1102 1 0.6936864020403171 1102 2 0.6922500465738075 1102 3 0.6609338031614733 1102 4 0.6354716355167156 1102 5 0.910188835828734 1102 6 0.8399267704207176 1102 7 0.8660613078269108 1102 8 0.8838300494946907 1102 9 1.0274784929132394 1102 10 1.111321769511214 1102 11 0.9877380164404386 1102 12 1.0566606476869538 1102 13 1.1159154217547353 1102 14 1.2498744551495804 1102 15 1.2893805539085434 1102 16 1.2603466926915203 1102 17 1.1772220387211334 1102 18 1.2350375490378696 1102 19 1.3389080574317944 1102 20 1.260555748876797 1102 21 1.339202782609263 1102 22 1.2671674291188992 1102 23 1.2828257287592564 1102 24 1.2762021707986264 1102 25 1.2885478168755427 1103 1 0.5280684769580759 1103 2 0.45942956348374153 1103 3 0.48927717795391956 1103 4 0.5717781623908138 1103 5 0.5212814149600433 1103 6 0.6351994803957339 1103 7 0.6033914540561227 1103 8 0.6412380597695346 1103 9 0.6977426925472585 1103 10 0.7321744090547924 1103 11 0.6761438846364456 1103 12 0.7463385331385126 1103 13 0.8127419936407279 1103 14 0.8631788310256994 1103 15 0.5813936174677821 1103 16 0.7885330907138552 1103 17 0.8149223290389642 1103 18 0.8942699683085493 1103 19 0.951865330317176 1103 20 0.9042107508018447 1103 21 0.6340130354518649 1103 22 0.9265540007670552 1103 23 0.9811343635221803 1103 24 0.9141967925604249 1103 25 0.8915416730782795 1104 1 0.5117922043253991 1104 2 0.5502647003095866 1104 3 0.48107474894294455 1104 4 0.476628930803912 1104 5 0.4854597235500293 1104 6 0.5609248228834645 1104 7 0.6570381576762472 1104 8 0.6469336231999421 1104 9 0.8138071619643767 1104 10 0.857599894810825 1104 11 0.8451189337956251 1104 12 0.7769108634090927 1104 13 0.8440238514951872 1104 14 0.8327502526261981 1104 15 0.9294856960363092 1104 16 0.8216171222548638 1104 17 0.7521689224053674 1104 18 0.8641981319377479 1104 19 0.7879161015437717 1104 20 0.8884848675960626 1104 21 0.7453240217435635 1104 22 0.882028287425868 1104 23 0.828154017963783 1104 24 0.986309676904348 1104 25 0.9712969256265751 1105 1 0.3743848767542559 1105 2 0.48857643424927844 1105 3 0.41426836961044344 1105 4 0.3629009037844355 1105 5 0.272536064133277 1105 6 0.49455676627540157 1105 7 0.5982197451525906 1105 8 0.6067645381550264 1105 9 0.5679434993578525 1105 10 0.5264942095229372 1105 11 0.6156782478616449 1105 12 0.4766049527763343 1105 13 0.5669971151783031 1105 14 0.805461455966217 1105 15 0.7056916762947836 1105 16 0.828170270398644 1105 17 0.5745169095693257 1105 18 0.7906100741491926 1105 19 0.8408011580035861 1105 20 0.8430995835788693 1105 21 0.7479001226618536 1105 22 0.7192242586908648 1105 23 0.731513082037381 1105 24 0.9080352703030172 1105 25 0.8386684111982845 1106 1 0.334951804724831 1106 2 0.3723031414133331 1106 3 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0.6328556222084032 1107 16 0.6047255868636299 1107 17 0.42199975370822784 1107 18 0.7053945080651474 1107 19 0.7650866125342191 1107 20 0.753388492497649 1107 21 0.6423695926546529 1107 22 0.7453190314211184 1107 23 0.6838206211172277 1107 24 0.7913836378689112 1107 25 0.723938844846991 1108 1 0.5441261143225589 1108 2 0.5078468112556322 1108 3 0.46145566107320946 1108 4 0.537001050826369 1108 5 0.628858492739802 1108 6 0.5292284680014063 1108 7 0.4775215199276417 1108 8 0.5307367105367149 1108 9 0.5988229406578611 1108 10 0.7531161744253154 1108 11 0.7599156863168872 1108 12 0.7163520383945254 1108 13 0.7549046789717124 1108 14 0.8491469820639352 1108 15 0.7815773647928026 1108 16 0.6933106935352171 1108 17 0.7230628871240583 1108 18 0.7374405059935611 1108 19 0.6184647723527377 1108 20 0.7438481170097475 1108 21 0.9498451255604508 1108 22 0.9235767252779326 1108 23 0.866295301874608 1108 24 0.8279596568123466 1108 25 0.830565916176333 1109 1 0.16499062415934773 1109 2 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2216 19 -2.250967093707999 2216 20 -2.667814337815585 2216 21 -2.5330061692236328 2216 22 -2.8241891643107806 2216 23 -1.9453361468213757 2216 24 -2.227422071946272 2216 25 -2.063488154784096 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 58572.0 3 4.0 4 943.0 5 8448.0 6 9850.0 7 7507.0 8 6214.0 9 5599.0 10 4789.0 11 4142.0 12 4051.0 13 3808.0 14 3683.0 15 3699.0 16 3768.0 17 4036.0 18 4488.0 19 5244.0 20 6543.0 21 8455.0 22 10744.0 23 13360.0 24 15893.0 25 18205.0 26 20857.0 27 22922.0 28 26065.0 29 31247.0 30 39544.0 31 50398.0 32 65135.0 33 83524.0 34 109189.0 35 143693.0 36 197104.0 37 309140.0 38 1046386.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.22411927214307 18.898228842980224 13.151362025140182 27.726289859736525 2 14.118260935965502 20.533207197385085 39.17123591311043 26.17729595353898 3 17.02751809418521 24.49855742859579 30.153623991356028 28.32030048586297 4 11.559326176640575 17.137701976846127 37.51390785468106 33.78906399183224 5 12.971149327352546 37.78130987207621 35.94092742098107 13.306613379590186 6 32.072964350824634 37.4816481003608 17.690169835208643 12.755217713605921 7 27.653518553018596 31.64985365443775 22.40888572399783 18.287742068545825 8 25.31809678088987 36.37622890657402 20.388376716245318 17.91729759629079 9 26.14814714211016 14.892723025417801 19.64043793588168 39.318691896590366 10 14.250374150411012 28.991985282001536 34.8672933933786 21.89034717420885 11 34.82151152554669 22.249873258228668 24.20218441298939 18.726430803235257 12 22.573351650915978 24.73997999095435 31.77021503359874 20.916453324530938 13 28.63772105145037 21.575614306981457 25.842703302847962 23.943961338720207 14 21.19186220408468 21.178073787966404 27.569704686067464 30.060359321881453 15 23.17112129989109 29.740409552751967 23.383419761660612 23.705049385696327 16 22.831312751158443 27.88882859129788 26.43734441488967 22.84251424265401 17 22.16266816148255 27.884709637531195 27.220402030222495 22.73222017076376 18 22.06175507961726 27.58937984019277 28.261019889997307 22.087845190192663 19 23.441349323823548 26.95699172433162 27.23024532581918 22.371413626025653 20 23.528590719741242 27.02278339883018 27.3905641270182 22.058061754410378 21 23.4126477414505 26.591615713780886 26.93595866018056 23.059777884588055 22 23.216846227857467 27.127286796186027 27.052962208152962 22.60290476780354 23 22.84095691246742 26.8849947056907 27.147037562445696 23.127010819396176 24 22.957934810367693 26.80089392452039 27.486312417117265 22.75485884799465 25 22.991637337387957 26.81524760472843 27.227908010711268 22.96520704717234 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 141.0 1 141.0 2 259.5 3 378.0 4 378.0 5 378.0 6 1009.0 7 1640.0 8 1640.0 9 1640.0 10 2623.0 11 3606.0 12 3606.0 13 3606.0 14 6542.0 15 9478.0 16 9478.0 17 9478.0 18 17701.0 19 25924.0 20 25924.0 21 25924.0 22 40449.0 23 54974.0 24 54974.0 25 54974.0 26 78203.5 27 101433.0 28 101433.0 29 101433.0 30 129785.0 31 158137.0 32 158137.0 33 158137.0 34 183470.5 35 208804.0 36 208804.0 37 208804.0 38 232276.0 39 255748.0 40 255748.0 41 255748.0 42 273447.0 43 291146.0 44 291146.0 45 291146.0 46 299950.5 47 308755.0 48 308755.0 49 308755.0 50 302027.0 51 295299.0 52 295299.0 53 295299.0 54 269708.0 55 244117.0 56 244117.0 57 244117.0 58 211356.5 59 178596.0 60 178596.0 61 178596.0 62 145707.0 63 112818.0 64 112818.0 65 112818.0 66 87398.0 67 61978.0 68 61978.0 69 61978.0 70 44990.0 71 28002.0 72 28002.0 73 28002.0 74 19393.0 75 10784.0 76 10784.0 77 10784.0 78 7334.0 79 3884.0 80 3884.0 81 3884.0 82 2532.0 83 1180.0 84 1180.0 85 1180.0 86 767.0 87 354.0 88 354.0 89 354.0 90 209.0 91 64.0 92 64.0 93 64.0 94 36.0 95 8.0 96 8.0 97 8.0 98 4.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08043274172563017 2 1.6968932853508474E-4 3 0.0 4 0.028295695533225383 5 0.07237249862021365 6 0.08089938737910166 7 0.035634758992367795 8 0.06779088674976635 9 0.04687667700781717 10 0.10949203923726344 11 0.003987699220574492 12 0.013363034622137924 13 0.006872417805670932 14 0.008399621762486695 15 0.008060243105416526 16 0.017817379496183897 17 0.027998739208288985 18 0.0014423592925482205 19 0.004157388549109576 20 0.005854281834460425 21 2.545339928026271E-4 22 8.90868974809195E-4 23 4.2422332133771184E-5 24 1.2726699640131355E-4 25 0.004454344874045974 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2357249.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.84505087128099 #Duplication Level Percentage of deduplicated Percentage of total 1 83.22746326872014 61.459362589663094 2 11.46770559762526 16.93666606467022 3 2.856727497197739 6.328655620678628 4 1.0250293760740021 3.0277338568296837 5 0.47709836336268585 1.761567645656122 6 0.25382999540434253 1.1246453353974417 7 0.16689557336695116 0.8627088473831881 8 0.10857383126786042 0.6414112074612025 9 0.07506132211166214 0.498861643482117 >10 0.31406644307334836 3.9200077497917034 >50 0.016129778623438014 0.8152851161335692 >100 0.009979844400792776 1.4534750314936782 >500 9.210296138788722E-4 0.4667424975156351 >1k 5.1807915780542E-4 0.7028767938437048 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3715 0.15759896387695996 No Hit TATCAACGCAGAGTACTTTTTTTTT 2489 0.10558918468095649 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACCG 60 4.0427144E-4 12.685394 5 GGTATCA 865 0.0 12.308034 1 CACGACT 60 0.0058129015 11.09972 4 ATCGTGC 60 0.0058609224 11.08746 8 TAGGACC 460 0.0 10.961836 4 ATACCGT 70 0.0014767605 10.870421 6 GTACTAG 145 1.4308171E-8 10.489113 1 GTATCAA 1895 0.0 10.483923 1 TGGCGAG 830 0.0 10.176272 18 CGTACTG 75 0.0026161743 10.148315 5 ACGACTC 75 0.0026161743 10.148315 5 GACGTGG 480 0.0 10.098366 7 ACACCGC 95 1.6244776E-4 10.012231 6 TCCAACG 105 4.1533327E-5 9.942147 18 TGTACCG 115 1.0089843E-5 9.9277 5 GTCCTAC 1065 0.0 9.818154 1 TGTAGGA 1125 0.0 9.801702 2 GTAGGAC 1055 0.0 9.641525 3 GGACGTG 870 0.0 9.620948 6 GGGCCGT 90 0.0011015772 9.511619 6 >>END_MODULE