FastQCFastQC Report
Thu 26 May 2016
SRR1512977_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512977_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1860528
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27120.14576507314052786No Hit
GTCCTAAAGTGTGTATTTCTCATTT25360.13630539287772075No Hit
GTCCTACAGTGGACATTTCTAAATT24920.13394047281201896No Hit
CTGTAGGACGTGGAATATGGCAAGA23280.12512577074894868No Hit
CTTTAGGACGTGAAATATGGCGAGG21460.11534360138627314No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG400.00528845214.246414
GTGCGCC1001.0157237E-813.2923611
TAACGTG550.003074632512.0878635
GGCGTGC1251.8479113E-811.3934518
TCCAACG1850.011.30052918
TAGGACC5300.010.7520084
GGTATCA8350.010.7274011
GCGCCAC1252.230754E-710.6433313
GCCTAAG1355.4380507E-810.5879191
GGCGAGG6150.010.49774919
TAGGACA5950.010.2159124
GGACCTG10350.010.1820056
CCAACGA1502.467641E-810.12723919
GTAGGAC14600.010.0830763
GTCCTAC14100.010.0697871
GTCCTAA8050.010.06181051
TGTAGGA14950.09.9743432
TTAGGAC9150.09.9647023
TGGCGAG14500.09.9614718
GCGTGCG1252.5889585E-69.8743249
Produced by FastQC (version 0.11.5)