##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512977_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1860528
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.010843695983077	31.0	30.0	33.0	23.0	34.0
2	29.14910713517883	31.0	30.0	34.0	23.0	34.0
3	29.438514228219088	31.0	30.0	34.0	24.0	34.0
4	32.89776773045071	35.0	33.0	37.0	26.0	37.0
5	33.0875837396696	37.0	35.0	37.0	27.0	37.0
6	33.09790715323822	37.0	35.0	37.0	26.0	37.0
7	32.8983809972223	37.0	35.0	37.0	26.0	37.0
8	32.95749539915551	37.0	35.0	37.0	26.0	37.0
9	34.2975606924486	39.0	35.0	39.0	26.0	39.0
10	34.14880077053395	39.0	35.0	39.0	25.0	39.0
11	34.24800970477198	39.0	35.0	39.0	26.0	39.0
12	34.13192007860135	39.0	35.0	39.0	25.0	39.0
13	34.14769463292141	39.0	35.0	39.0	25.0	39.0
14	35.16539928450418	39.0	35.0	41.0	25.0	41.0
15	35.10173509885366	39.0	35.0	41.0	25.0	41.0
16	34.999819943585905	39.0	35.0	41.0	24.0	41.0
17	34.95187011429014	39.0	35.0	41.0	24.0	41.0
18	34.632016287849474	39.0	34.0	41.0	18.0	41.0
19	34.87334831832684	39.0	34.0	41.0	22.0	41.0
20	34.94536819655496	39.0	35.0	41.0	23.0	41.0
21	34.89131096118951	39.0	35.0	41.0	22.0	41.0
22	34.818223644040835	39.0	35.0	41.0	22.0	41.0
23	34.54927203460523	39.0	34.0	41.0	18.0	41.0
24	34.55569547999278	39.0	34.0	41.0	18.0	41.0
25	34.47908550690987	39.0	34.0	41.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6771528940179365
1101	2	0.9639439203916247
1101	3	1.0538248595561797
1101	4	1.0156178655375143
1101	5	0.883798557819965
1101	6	1.068571217102125
1101	7	1.0991128190868977
1101	8	0.9554676232614199
1101	9	0.9036337057537267
1101	10	0.9142016400787583
1101	11	0.9121709528176822
1101	12	1.1314784520175607
1101	13	1.0310969772085272
1101	14	0.8310625542388834
1101	15	0.8281195419151999
1101	16	1.293328611265828
1101	17	1.2461040896371856
1101	18	1.4659981164943474
1101	19	0.8238625239965032
1101	20	1.0852159379257742
1101	21	1.0392014042775628
1101	22	1.1833591451505185
1101	23	1.3918741834332593
1101	24	1.122429541727655
1101	25	1.2690120801383458
1102	1	0.4706396861929285
1102	2	0.5708999153571774
1102	3	0.6623128838789469
1102	4	0.6999076735071839
1102	5	0.6083687897847199
1102	6	0.5255403065036433
1102	7	0.7679380916234422
1102	8	0.821819481957327
1102	9	0.8418540389301583
1102	10	0.9590698985240209
1102	11	0.816663320379476
1102	12	0.9968127570947303
1102	13	0.7799242096526484
1102	14	0.809791530594616
1102	15	0.9878231871205685
1102	16	0.9664612261323313
1102	17	0.8164124331445208
1102	18	1.116109022895266
1102	19	1.1159221602109284
1102	20	1.0523015435706213
1102	21	0.8078174147573023
1102	22	1.1516675968720094
1102	23	1.204860221842992
1102	24	1.1915979028977475
1102	25	1.078730601513847
1103	1	0.2971923728906205
1103	2	0.43858168856480617
1103	3	0.4649603393853603
1103	4	0.6128512456370885
1103	5	0.14842101527649731
1103	6	0.6458757459392857
1103	7	0.42396237819041716
1103	8	0.4796783879683204
1103	9	0.730863152943634
1103	10	0.7420999143241929
1103	11	0.6551408444961027
1103	12	0.7969070139283474
1103	13	0.7965345514993345
1103	14	1.041634618288235
1103	15	1.0678354221161328
1103	16	1.11176930941798
1103	17	0.9013533103503732
1103	18	1.127007642502555
1103	19	1.3138829270952144
1103	20	1.1600889317996845
1103	21	1.0768743147934074
1103	22	1.1068144709893701
1103	23	1.4522962520404548
1103	24	1.1959307945517708
1103	25	1.0482544772661555
1104	1	0.7266305266348496
1104	2	0.8017170528254525
1104	3	0.79986074780415
1104	4	1.0243767044243626
1104	5	0.6717612659235712
1104	6	0.797250627621743
1104	7	0.9552797729736824
1104	8	0.9702367168612298
1104	9	1.0966952453323628
1104	10	1.1404338884271112
1104	11	1.2211331167899502
1104	12	1.0387458202832818
1104	13	1.1213821220333244
1104	14	1.2206326645030856
1104	15	1.40438359512342
1104	16	1.3002576153106418
1104	17	1.3129453442229746
1104	18	1.6445181390078005
1104	19	1.3808299835396411
1104	20	1.43208050611684
1104	21	1.5824346459351375
1104	22	1.528069010398454
1104	23	1.7882752268041244
1104	24	1.5580837703581594
1104	25	1.3644726426966258
1105	1	0.5256178825475466
1105	2	0.6043471199961701
1105	3	0.74315809294513
1105	4	0.7772045551744995
1105	5	0.5076795331791999
1105	6	0.345627802757285
1105	7	0.5593256702291143
1105	8	0.6504080402877861
1105	9	0.5510653841887603
1105	10	0.750490338876368
1105	11	0.7451202093505103
1105	12	0.7853470465978205
1105	13	0.7474650811412289
1105	14	0.9110115167630326
1105	15	1.0110946160061474
1105	16	1.0001666302095131
1105	17	1.0434594296426454
1105	18	1.0251490670337802
1105	19	1.1260088633351941
1105	20	1.1685416952387015
1105	21	1.0929034445863834
1105	22	0.812450734299091
1105	23	0.8829720007037025
1105	24	1.0826237799172276
1105	25	0.968395928096605
1106	1	0.44236600306360785
1106	2	0.5375116115905136
1106	3	0.5783066380393826
1106	4	0.6006239845902854
1106	5	0.5935809695669292
1106	6	0.6132616861586442
1106	7	0.4291604302775269
1106	8	0.5035617837921649
1106	9	0.6333307493756379
1106	10	0.682167184092414
1106	11	0.7402780802741589
1106	12	0.7726101781674259
1106	13	0.7050511708601022
1106	14	0.8391179152174431
1106	15	1.01410331756815
1106	16	0.9027572921620362
1106	17	0.8814909160099447
1106	18	1.0598758498230936
1106	19	0.6981504059438279
1106	20	0.7924069890261904
1106	21	0.7634649416063155
1106	22	0.7280806143710237
1106	23	0.9651698323767732
1106	24	0.8853249772888176
1106	25	0.9131394343793673
1107	1	0.08045179194408547
1107	2	0.2894235961735383
1107	3	0.3397702019853206
1107	4	0.4903333095349325
1107	5	0.3506743000650374
1107	6	0.13561799588655532
1107	7	0.3720844543679789
1107	8	0.41882359492465326
1107	9	0.4673100325739199
1107	10	0.3280547902462345
1107	11	0.2939520323716778
1107	12	0.6283470616265916
1107	13	0.6626373192284802
1107	14	0.7445269447526641
1107	15	0.8131552993286704
1107	16	0.6066056483392188
1107	17	0.7517468914236431
1107	18	1.0019710015994576
1107	19	0.8922784046164907
1107	20	0.6444032196476996
1107	21	0.6241147469869972
1107	22	0.5925667850524476
1107	23	0.5735371994568368
1107	24	0.6059009636873967
1107	25	0.6872312029645116
1108	1	0.343055442257409
1108	2	0.3067339942923688
1108	3	0.19781145710249248
1108	4	0.4458987553225455
1108	5	0.36943996406075996
1108	6	0.5265298850162807
1108	7	0.44757091068810695
1108	8	0.4315802420658983
1108	9	0.4811280495785013
1108	10	0.4405588276219703
1108	11	0.26941950645508683
1108	12	0.4643581752881403
1108	13	0.4439673257058061
1108	14	0.5918175955046223
1108	15	0.6287773881106347
1108	16	0.5937463218990686
1108	17	0.6638131108104091
1108	18	0.7822885831807298
1108	19	0.7461842188463876
1108	20	0.7608508897095589
1108	21	0.5373555287748033
1108	22	0.5099993119619626
1108	23	0.6710837015562703
1108	24	0.7719609580686537
1108	25	0.8867730593805376
1109	1	-0.08333632751296705
1109	2	-0.04889684057840071
1109	3	0.23366548935215192
1109	4	0.40955135402241183
1109	5	0.1373126053571383
1109	6	0.09133217258094817
1109	7	0.1878610950761015
1109	8	0.29389379546962857
1109	9	0.40060917821232067
1109	10	0.5837915067201465
1109	11	0.06636617743004791
1109	12	0.3211432157826195
1109	13	0.373875218568287
1109	14	0.2248875670490449
1109	15	0.5860380780629271
1109	16	0.5284471908539956
1109	17	0.213257028299644
1109	18	-1.2582312610258342
1109	19	0.1735470917830142
1109	20	0.3646383657794203
1109	21	0.4954514716829692
1109	22	0.5798353536134258
1109	23	0.2028313050682229
1109	24	0.4418699627487115
1109	25	0.5945700275929795
1110	1	0.24529611971616205
1110	2	0.2941204151837269
1110	3	0.34615145694254323
1110	4	0.08820073950801088
1110	5	0.34535645164003625
1110	6	0.25930190648163176
1110	7	0.4257705768486417
1110	8	0.2962318756742377
1110	9	0.5842108662147183
1110	10	0.7746729477059233
1110	11	0.6597807774214459
1110	12	0.14418465188796148
1110	13	0.32975972600240055
1110	14	0.5521554794285706
1110	15	0.5834270908883994
1110	16	0.7431399382761654
1110	17	0.5106884214284904
1110	18	0.9482881421744622
1110	19	0.7426006430167362
1110	20	0.7182622514478041
1110	21	0.7215892118283946
1110	22	0.7572787259948726
1110	23	0.6994903321883825
1110	24	0.9327826921373017
1110	25	0.8810308378285825
1111	1	-0.026929697152581866
1111	2	0.3051396442592633
1111	3	0.282947041594511
1111	4	0.0422774796659553
1111	5	-0.015910142611794242
1111	6	0.15879531575268402
1111	7	0.054666915984945774
1111	8	-0.0055800610312815024
1111	9	0.32383591392044053
1111	10	0.1576926464993349
1111	11	0.009195567663056181
1111	12	0.2434050376437611
1111	13	0.24707951580364806
1111	14	0.13498952223161353
1111	15	0.3629988256152288
1111	16	0.20257557224851297
1111	17	0.31426908079495774
1111	18	0.42848706769481737
1111	19	0.23652777803126668
1111	20	0.16983492561038815
1111	21	0.3713424337994482
1111	22	0.1664301219376867
1111	23	0.6193453067942656
1111	24	0.6252619217999893
1111	25	0.19507998206132982
1112	1	-0.009849799725550668
1112	2	0.17676289029032333
1112	3	0.11015331122311522
1112	4	0.2339989191450229
1112	5	0.09342330597206683
1112	6	0.0826056133871731
1112	7	-0.23935861200652653
1112	8	0.04254221977662809
1112	9	0.3055090058039198
1112	10	0.3516967378930005
1112	11	0.25525474880807053
1112	12	0.32148680789183715
1112	13	0.16015480320965025
1112	14	0.25804930844189045
1112	15	0.2639834108573993
1112	16	0.3881581924692199
1112	17	0.5143770705376625
1112	18	0.4503522822048609
1112	19	0.2685397640859506
1112	20	0.23787941231336163
1112	21	0.25978810758283544
1112	22	0.23033550125597912
1112	23	0.5336628796181913
1112	24	0.2584373619129323
1112	25	0.4324734761691431
1113	1	-0.16543816290640123
1113	2	-0.007855483052370715
1113	3	-0.2615490761597137
1113	4	-0.013224204809397122
1113	5	-0.22310309817923724
1113	6	-0.050671985182809465
1113	7	-0.2515014737144483
1113	8	0.05793319065681146
1113	9	-0.08891196421897263
1113	10	-0.11592398715258412
1113	11	-0.1798177780086334
1113	12	0.05087502795622356
1113	13	-0.22175129925107484
1113	14	-0.04559595224099411
1113	15	-0.33516551555970864
1113	16	-0.30395891973396516
1113	17	-0.3893427235456386
1113	18	-0.5524581470938656
1113	19	-0.40488978388947316
1113	20	-0.5674626843142789
1113	21	-0.46647321374758377
1113	22	-0.22628438479299007
1113	23	-0.6258281291016914
1113	24	-0.16174578485549773
1113	25	-0.05913600671847519
1114	1	-1.1542458165091105
1114	2	-1.0527401220079433
1114	3	-0.8646122617346244
1114	4	-0.9993236748495313
1114	5	-0.919601592688899
1114	6	-1.3157579744749626
1114	7	-1.598106439608653
1114	8	-0.9839476100911142
1114	9	-1.091132945288095
1114	10	-1.2517274075478397
1114	11	-1.8816422766754286
1114	12	-1.375991841202854
1114	13	-1.267453966560275
1114	14	-1.7180956657308641
1114	15	-1.5338675137500672
1114	16	-2.2760198804319174
1114	17	-4.232636563031356
1114	18	-3.168178533120134
1114	19	-2.130089180329776
1114	20	-1.2000254289503758
1114	21	-2.395161789520067
1114	22	-2.5700940507302974
1114	23	-2.427955305834665
1114	24	-1.85856439153212
1114	25	-1.641607690373199
1115	1	-0.8815276973196973
1115	2	-0.677953356325645
1115	3	-0.8960855740643474
1115	4	-0.7634795879885559
1115	5	-0.749577412756345
1115	6	-0.7941156944692978
1115	7	-1.8055920038824738
1115	8	-1.4310815511574546
1115	9	-1.1592431602516342
1115	10	-0.9412071668867341
1115	11	-1.5680123536805581
1115	12	-1.2514885367093527
1115	13	-1.1307213364928188
1115	14	-1.066644213110834
1115	15	-2.5698455606477566
1115	16	-1.18916985292487
1115	17	-1.2009137172999118
1115	18	-1.219297128611899
1115	19	-1.3063750434599726
1115	20	-0.9873252002843813
1115	21	-1.707911778940435
1115	22	-1.7645155929050844
1115	23	-1.2768227014501718
1115	24	-1.1934863722144442
1115	25	-1.5016873075802053
1116	1	-1.1355737772605003
1116	2	-2.0237077148773963
1116	3	-1.631498013920691
1116	4	-1.4663542522008086
1116	5	-1.8856801955328244
1116	6	-1.5760579580877199
1116	7	-1.276445359619494
1116	8	-2.0122076422287485
1116	9	-1.9697956104128593
1116	10	-2.979468814536258
1116	11	-1.5914616908198695
1116	12	-1.6934379632901368
1116	13	-2.2358960990142407
1116	14	-1.9525583661153831
1116	15	-1.5395250926165858
1116	16	-1.588232666830038
1116	17	-1.898642821249247
1116	18	-1.735815516096821
1116	19	-2.1439443606010897
1116	20	-2.4682479840087908
1116	21	-2.7819008991879954
1116	22	-2.065506787889028
1116	23	-1.9919424383546414
1116	24	-2.5508514952385113
1116	25	-2.281670458947282
1201	1	0.24789303034145505
1201	2	0.32298484931630966
1201	3	0.457624289756545
1201	4	0.6435962066406518
1201	5	0.7055760706085863
1201	6	0.6872550590053592
1201	7	0.5955490751293411
1201	8	0.12639780222074393
1201	9	0.5178987212625685
1201	10	0.6789578817428534
1201	11	0.2947475419558714
1201	12	-0.32038573438343576
1201	13	0.18337346065653293
1201	14	0.5866054074580944
1201	15	0.3518303493830217
1201	16	0.7521170299631024
1201	17	0.8286545263700447
1201	18	1.1400643272173525
1201	19	0.7879703993857632
1201	20	0.7970436504752811
1201	21	0.7725580723407575
1201	22	0.9157003558017109
1201	23	1.1710164365149538
1201	24	1.1319195000657274
1201	25	1.009811241291736
1202	1	0.47398093790889817
1202	2	0.5860061672659818
1202	3	0.4187178617238061
1202	4	0.7700252296976444
1202	5	0.7347961572994777
1202	6	0.644797090375576
1202	7	0.6624197363677595
1202	8	0.7152793634838659
1202	9	1.0277281689591007
1202	10	1.1461861184222641
1202	11	0.803688152274546
1202	12	0.8609475652137988
1202	13	0.9724291165134957
1202	14	1.0707382863335297
1202	15	0.7350414165646555
1202	16	0.9587337672035119
1202	17	1.0622499402194734
1202	18	1.391527630503795
1202	19	1.1736972545175135
1202	20	1.0426153794068114
1202	21	1.0834438408218787
1202	22	1.2483800522203552
1202	23	1.1758404265813098
1202	24	1.3283599063354146
1202	25	1.1505061450731162
1203	1	0.08364620638501208
1203	2	0.32653094860708975
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2211	19	-0.15552521014969756
2211	20	-0.2197959193444916
2211	21	-0.12167989780204636
2211	22	-0.22270863782289751
2211	23	0.36944344430831677
2211	24	0.09836008969717369
2211	25	0.15021454894979058
2212	1	-0.2958614200717733
2212	2	-0.35015699227298924
2212	3	-0.2958097459505815
2212	4	-0.5140817381050695
2212	5	-0.40096063113978175
2212	6	-0.49672670086749093
2212	7	-0.5413727504431733
2212	8	-0.5063170153623133
2212	9	-0.3958675517803698
2212	10	-0.4014579428012439
2212	11	-0.6079017728168523
2212	12	-0.3934463008791127
2212	13	-0.6226972948169021
2212	14	-0.612488855475732
2212	15	-0.7228328987644304
2212	16	-0.5215142175895195
2212	17	-0.6140897280438224
2212	18	-3.117745235031883
2212	19	-1.4061513123865765
2212	20	-0.9022925444134202
2212	21	-0.8591412454224141
2212	22	-0.7348917223467595
2212	23	-0.5989345489110747
2212	24	-1.0051018818541166
2212	25	-0.6733395240069342
2213	1	0.07426835745892646
2213	2	0.10247434483350659
2213	3	-0.18108823322091183
2213	4	-0.19212202812732215
2213	5	-0.05515378494156664
2213	6	-0.11680283261248547
2213	7	-0.11434878775347812
2213	8	-0.5217179961068723
2213	9	-0.21851062305366042
2213	10	-0.24848630580348896
2213	11	-0.31210479563237925
2213	12	-0.3163311629890728
2213	13	-0.17865867709451067
2213	14	-0.23674562367251895
2213	15	-0.39948881062056074
2213	16	-0.8285413854451491
2213	17	-0.5223256689999545
2213	18	-0.2747012599742291
2213	19	-0.11923690027648348
2213	20	-0.9317231430641684
2213	21	-0.6944936144836902
2213	22	-0.48587375712008907
2213	23	-0.9106054725330566
2213	24	-0.6148025338428127
2213	25	-0.6993412062663396
2214	1	-1.4466330219607642
2214	2	-3.5042648991437737
2214	3	-1.7721292334167735
2214	4	-1.4820498860629456
2214	5	-1.4409506119964774
2214	6	-1.5267374057758225
2214	7	-1.6310144440270875
2214	8	-1.3784730954829136
2214	9	-1.546332884033525
2214	10	-1.6606765426085346
2214	11	-1.3462134890542572
2214	12	-1.9953547035928878
2214	13	-1.798326832925099
2214	14	-2.9293318257449172
2214	15	-3.173828013560339
2214	16	-2.3471707794891117
2214	17	-2.8405815285024687
2214	18	-2.2292237793038296
2214	19	-4.378781383996717
2214	20	-2.706692831272413
2214	21	-2.7357789743546093
2214	22	-2.7039258281149685
2214	23	-2.7402424771684295
2214	24	-2.9136548827735034
2214	25	-2.69664019245349
2215	1	-1.095964572762906
2215	2	-2.6691241316068144
2215	3	-1.468767327156943
2215	4	-1.074563255046904
2215	5	-1.0157972618863766
2215	6	-1.4727817593461268
2215	7	-1.8932038117527874
2215	8	-1.8362467664486246
2215	9	-1.583030744996094
2215	10	-3.2509565048608167
2215	11	-2.2418842974845816
2215	12	-2.4413951251090857
2215	13	-1.8166069524595443
2215	14	-3.0679288688402266
2215	15	-2.7696718323712872
2215	16	-2.554570905314847
2215	17	-2.7730445671938853
2215	18	-1.9167395075202478
2215	19	-2.55040374952047
2215	20	-2.6158899058241616
2215	21	-2.2315087635965085
2215	22	-1.8594936167277467
2215	23	-2.277054367700039
2215	24	-2.2935424260261
2215	25	-2.1867235520005153
2216	1	-1.8758466323327312
2216	2	-1.899808480330993
2216	3	-2.4614277892768683
2216	4	-3.1649104797710734
2216	5	-2.3559979081611644
2216	6	-2.3531044764109055
2216	7	-2.2070768454355942
2216	8	-2.8437349200791395
2216	9	-3.0813326009559034
2216	10	-2.6001095880907847
2216	11	-3.0943224750480205
2216	12	-3.7072053318586846
2216	13	-4.230757981232241
2216	14	-3.524898497185049
2216	15	-3.3655426091534295
2216	16	-2.5304284589408965
2216	17	-2.5451773769193977
2216	18	-2.646196114294554
2216	19	-3.0057547957925053
2216	20	-3.0155780272009842
2216	21	-3.3126180075390756
2216	22	-3.6347578653968498
2216	23	-3.672527313199879
2216	24	-3.5851890413613567
2216	25	-3.7541073915281054
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	81475.0
3	5.0
4	655.0
5	4021.0
6	4848.0
7	4451.0
8	4041.0
9	3869.0
10	3496.0
11	3369.0
12	3194.0
13	3164.0
14	3287.0
15	3325.0
16	3413.0
17	3623.0
18	4172.0
19	4918.0
20	6254.0
21	8172.0
22	10261.0
23	12756.0
24	14334.0
25	16486.0
26	18563.0
27	20911.0
28	23665.0
29	28322.0
30	34810.0
31	43368.0
32	54714.0
33	70224.0
34	92095.0
35	122158.0
36	168761.0
37	266711.0
38	708637.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.41583319938162	18.622019934171277	12.350383137835765	25.61176372861133
2	14.920354125051416	20.3574587521743	41.15173984229445	23.570447280479836
3	18.448537559863023	24.906996237911123	29.79416314357286	26.85030305865299
4	12.20933608619911	16.937221756242337	38.70916403208809	32.14427812547046
5	12.99750080116314	37.8686694669738	36.32323115008313	12.810598581779937
6	31.410120640477185	37.630507494202355	18.3309339987679	12.628437866552558
7	27.608230309324682	31.653985548147933	22.761935786987312	17.975848355540077
8	24.698437001046155	36.99502511098615	20.922219916544005	17.384317971423688
9	26.4368267163676	15.11837750168934	20.100441190779666	38.34435459116339
10	14.612446544582692	28.88116568424278	35.188783971572875	21.317603799601656
11	34.208487774196925	22.375114570705918	24.98020379474305	18.4361938603541
12	22.961269797581124	24.696618969337738	32.222038251759756	20.120072981321382
13	28.907628799253416	22.02262013621109	25.75130321938328	23.318447845152217
14	20.83088494219288	21.964232432737564	27.87746664767091	29.32741597739864
15	22.89020473511098	30.655322241946774	23.579912927633593	22.874560095308652
16	22.33555013555089	27.973854241535932	27.61910496095285	22.07149066196033
17	21.74344048714063	28.340450100824604	27.919357517978284	21.996751894056484
18	21.621785958734574	27.569833965415558	29.467318263804287	21.341061812045577
19	23.390360089478047	26.980449121320603	27.422624657213156	22.206566131988186
20	23.42406368470737	27.38532731522597	27.990596528048066	21.200012472018596
21	23.073664358724994	26.909051614994684	27.02947531287313	22.98780871340719
22	23.04088505898512	27.995688670395623	27.176966103380522	21.786460167238733
23	22.47384361973553	27.690927329828973	27.494976233201697	22.340252817233804
24	23.008821489815777	26.898716825338802	28.088999747343067	22.00346193750235
25	22.738642747512976	27.274501318438517	27.830207697745617	22.15664823630289
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	241.0
1	241.0
2	362.0
3	483.0
4	483.0
5	483.0
6	961.0
7	1439.0
8	1439.0
9	1439.0
10	2201.0
11	2963.0
12	2963.0
13	2963.0
14	5455.0
15	7947.0
16	7947.0
17	7947.0
18	14830.5
19	21714.0
20	21714.0
21	21714.0
22	34369.0
23	47024.0
24	47024.0
25	47024.0
26	68217.5
27	89411.0
28	89411.0
29	89411.0
30	114977.5
31	140544.0
32	140544.0
33	140544.0
34	160267.5
35	179991.0
36	179991.0
37	179991.0
38	194785.5
39	209580.0
40	209580.0
41	209580.0
42	219433.5
43	229287.0
44	229287.0
45	229287.0
46	234240.5
47	239194.0
48	239194.0
49	239194.0
50	231958.0
51	224722.0
52	224722.0
53	224722.0
54	201360.5
55	177999.0
56	177999.0
57	177999.0
58	153522.5
59	129046.0
60	129046.0
61	129046.0
62	105095.5
63	81145.0
64	81145.0
65	81145.0
66	62803.5
67	44462.0
68	44462.0
69	44462.0
70	32481.5
71	20501.0
72	20501.0
73	20501.0
74	14408.0
75	8315.0
76	8315.0
77	8315.0
78	5681.5
79	3048.0
80	3048.0
81	3048.0
82	2033.5
83	1019.0
84	1019.0
85	1019.0
86	677.5
87	336.0
88	336.0
89	336.0
90	207.0
91	78.0
92	78.0
93	78.0
94	54.5
95	31.0
96	31.0
97	31.0
98	19.5
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3561354626213634
2	0.039397418367259185
3	0.035312556435592474
4	0.03482882278578984
5	0.03934367018394778
6	0.015586973160307181
7	0.015156987693815948
8	0.020746798758201974
9	0.018005641409320365
10	0.017898145042697558
11	0.017360663209583516
12	0.017521907759517726
13	0.017575655942829133
14	0.02488540887318009
15	0.025046653423114296
16	0.01832813050918879
17	0.019725583275285296
18	0.11394614862017664
19	0.02187551060774146
20	0.019564338725351085
21	0.02305797064059235
22	0.016823181376469477
23	0.019886827825219507
24	0.016016958626798417
25	0.02004807237515372
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1860528.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.79558574091175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.7860031789866	60.264555925633125
2	11.710022308424284	17.048758659617786
3	3.0240392794759026	6.604101319589191
4	1.0933413441164048	3.1836169423883773
5	0.47374390542829586	1.7243232543419948
6	0.26552277376347655	1.1597331506198323
7	0.15351856923759066	0.782283191882999
8	0.10100423807916228	0.5882130138629698
9	0.06761097746556742	0.4429602636409408
>10	0.2943790091735686	3.627418005749049
>50	0.016240529563894882	0.8203635967241355
>100	0.012059467321109293	1.8009199744722255
>500	0.0017009304758277154	0.8358942242394828
>1k	8.134884884393371E-4	1.1168584772378585
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2712	0.14576507314052786	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2536	0.13630539287772075	No Hit
GTCCTACAGTGGACATTTCTAAATT	2492	0.13394047281201896	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2328	0.12512577074894868	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2146	0.11534360138627314	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.0749636662280815E-4	0.0	0.0	0.0	0.0
7	1.0749636662280815E-4	0.0	0.0	0.0	0.0
8	1.0749636662280815E-4	0.0	0.0	0.0	0.0
9	1.0749636662280815E-4	0.0	0.0	0.0	0.0
10	1.0749636662280815E-4	0.0	0.0	0.0	0.0
11	1.612445499342122E-4	0.0	0.0	0.0	0.0
12	1.612445499342122E-4	0.0	0.0	5.3748183311404075E-5	0.0
13	1.612445499342122E-4	0.0	0.0	5.3748183311404075E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACG	40	0.005288452	14.24641	4
GTGCGCC	100	1.0157237E-8	13.29236	11
TAACGTG	55	0.0030746325	12.087863	5
GGCGTGC	125	1.8479113E-8	11.393451	8
TCCAACG	185	0.0	11.300529	18
TAGGACC	530	0.0	10.752008	4
GGTATCA	835	0.0	10.727401	1
GCGCCAC	125	2.230754E-7	10.64333	13
GCCTAAG	135	5.4380507E-8	10.587919	1
GGCGAGG	615	0.0	10.497749	19
TAGGACA	595	0.0	10.215912	4
GGACCTG	1035	0.0	10.182005	6
CCAACGA	150	2.467641E-8	10.127239	19
GTAGGAC	1460	0.0	10.083076	3
GTCCTAC	1410	0.0	10.069787	1
GTCCTAA	805	0.0	10.0618105	1
TGTAGGA	1495	0.0	9.974343	2
TTAGGAC	915	0.0	9.964702	3
TGGCGAG	1450	0.0	9.96147	18
GCGTGCG	125	2.5889585E-6	9.874324	9
>>END_MODULE