FastQCFastQC Report
Thu 26 May 2016
SRR1512977_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512977_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1860528
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33570.18043265137638348No Hit
GTCCTACAGTGGACATTTCTAAATT24360.13093057454658033No Hit
CTGTAGGACGTGGAATATGGCAAGA23970.12883439539743557No Hit
GTCCTAAAGTGTGTATTTCTCATTT23470.12614698623186538No Hit
CTTTAGGACGTGAAATATGGCGAGG22160.11910597421807143No Hit
TATCAACGCAGAGTACTTTTTTTTT22110.11883723330151441No Hit
GGTATCAACGCAGAGTACTTTTTTT19570.10518519474041778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG702.4576366E-816.3103125
GCACCGT853.886671E-612.3103656
CTAGGAC2400.012.2804333
TAAGACG550.00303434112.1091714
GCCTACG550.0030478412.1019951
TAGGACC4450.011.5455034
TCCAACG1406.9303496E-1011.52332918
ACCGTGC1201.268927E-711.0875248
GCGTGCG700.001488612310.8597889
GGTATCA9850.010.6188661
GGCGAGG6450.010.59326619
AGGACGT13200.010.4513675
AGGACCT9300.010.4350935
GGACGTG12900.010.3974436
AAGGCGT1659.076757E-1010.3773326
TGGCGAG13750.010.3525118
GACGTGA7850.010.1702717
CTAGAGT3000.010.1486384
AGGACCG750.002615039710.1486385
GGACCTG8650.010.0074276
Produced by FastQC (version 0.11.5)