FastQCFastQC Report
Thu 26 May 2016
SRR1512976_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512976_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800411
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT30850.17134976402610294No Hit
GTCCTAAAGTGTGTATTTCTCATTT29890.1660176481925516No Hit
GTATCAACGCAGAGTACTTTTTTTT29840.1657399338262208No Hit
CTGTAGGACGTGGAATATGGCAAGA25940.14407821325241849No Hit
CTTTAGGACGTGAAATATGGCGAGG25270.14035684074358576No Hit
GTCCTACAGTGTGCATTTCTCATTT20750.11525146202728154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC555.9432386E-717.26373510
GCGTGCG802.0116277E-613.05579
TAGGACC6500.013.0045354
GGCGAGG6550.011.59669219
AGACCCG855.338404E-511.1734475
AATCCCG1553.1650416E-1011.0262119
AAGGCGT1502.1518645E-910.7607646
AGGACCT11750.010.7502825
TGGCGAG15000.010.70638718
ACTAGAC1252.2477252E-710.6374173
GTCCTAA9550.010.5724961
GGACCTG11700.010.54976756
CTAGGAC2350.010.5080863
GTTATAA2100.010.43238451
TGTAGGA17550.010.3906392
GGTATCA8050.010.1760131
GTCCTAG2002.3646862E-1110.0014821
TAAAGTG10950.09.9744695
GCGCCAC1054.0910883E-59.95549813
GTCCTAC15350.09.9285691
Produced by FastQC (version 0.11.5)