##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512976_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1800411 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.158136669904817 31.0 30.0 34.0 25.0 34.0 2 29.291041878771015 31.0 30.0 34.0 23.0 34.0 3 29.578871157752314 31.0 30.0 34.0 25.0 34.0 4 33.02947327026995 35.0 35.0 37.0 26.0 37.0 5 33.2200586421656 37.0 35.0 37.0 27.0 37.0 6 33.23569229470382 37.0 35.0 37.0 27.0 37.0 7 33.04026525054557 37.0 35.0 37.0 26.0 37.0 8 33.09389245011278 37.0 35.0 37.0 26.0 37.0 9 34.45588868319511 39.0 35.0 39.0 27.0 39.0 10 34.30902999370699 39.0 35.0 39.0 26.0 39.0 11 34.407476403998864 39.0 35.0 39.0 27.0 39.0 12 34.29798473792928 39.0 35.0 39.0 26.0 39.0 13 34.310592970160705 39.0 35.0 39.0 26.0 39.0 14 35.3536731335234 40.0 36.0 41.0 25.0 41.0 15 35.290148749368896 40.0 36.0 41.0 25.0 41.0 16 35.19638071529223 40.0 36.0 41.0 25.0 41.0 17 35.15062060829444 40.0 35.0 41.0 25.0 41.0 18 34.84528254937345 39.0 35.0 41.0 21.0 41.0 19 35.077713921987815 40.0 35.0 41.0 24.0 41.0 20 35.15883484382177 40.0 35.0 41.0 25.0 41.0 21 35.105548677496415 40.0 35.0 41.0 24.0 41.0 22 35.033296841665596 40.0 35.0 41.0 24.0 41.0 23 34.76914715584386 40.0 35.0 41.0 19.0 41.0 24 34.77587228693893 40.0 35.0 41.0 19.0 41.0 25 34.714621828015936 40.0 35.0 41.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5643814552594115 1101 2 0.8277166127717805 1101 3 0.781467717436815 1101 4 0.8423794731379814 1101 5 0.7714923359708621 1101 6 0.9005955324257471 1101 7 1.0032565239080427 1101 8 0.7805327239238053 1101 9 0.5454523744349871 1101 10 0.6319303210034732 1101 11 0.8046178387739857 1101 12 1.0507388983635835 1101 13 0.8939765547039826 1101 14 0.8825300608217219 1101 15 0.6325751367227994 1101 16 1.1486634591545908 1101 17 1.077188336742644 1101 18 1.2258278983722946 1101 19 0.7565377983219221 1101 20 0.9313488206633806 1101 21 0.9739511593306105 1101 22 0.9559933695299563 1101 23 1.2873535389267303 1101 24 0.911643309436279 1101 25 1.1059727353098125 1102 1 0.47575817851732083 1102 2 0.5273964906694815 1102 3 0.6654544269185756 1102 4 0.8634686316033751 1102 5 0.7681102410046137 1102 6 0.7188531464754462 1102 7 0.8099666994300989 1102 8 0.7962885787885696 1102 9 0.9407771546607577 1102 10 0.9902341694063281 1102 11 0.9923947245706799 1102 12 0.9698919623186271 1102 13 0.9235915657546414 1102 14 0.9220510174218859 1102 15 1.1433668690491885 1102 16 1.0762618496242737 1102 17 0.9201563047106092 1102 18 1.1438089982840864 1102 19 1.1386798413550494 1102 20 1.2230699972855419 1102 21 0.8917278469092764 1102 22 1.2155598667202199 1102 23 1.4561634379346486 1102 24 1.4170447504812813 1102 25 1.354998989286564 1103 1 0.5201753057901044 1103 2 0.6932921625578565 1103 3 0.7372340745189625 1103 4 0.9000296426302583 1103 5 0.5002850779215038 1103 6 0.888166973944557 1103 7 0.6439878883236219 1103 8 0.8056258148063691 1103 9 1.0267470202033167 1103 10 0.9440685541943381 1103 11 0.9451240055959431 1103 12 1.2449137964858465 1103 13 1.1418590065338066 1103 14 1.255671558963222 1103 15 1.345839882882288 1103 16 1.3528436238610766 1103 17 1.2869143030475598 1103 18 1.5313219736032124 1103 19 1.5593102984628402 1103 20 1.3484215337887235 1103 21 1.1510733496106909 1103 22 1.2796954300741277 1103 23 1.5150747271742375 1103 24 1.4721189488592827 1103 25 1.4221186567188937 1104 1 0.524128851626525 1104 2 0.5600068661887967 1104 3 0.7135922851247649 1104 4 0.9229802526824216 1104 5 0.7929062200608072 1104 6 0.8080160894941955 1104 7 0.9815270479532145 1104 8 0.9066710507663558 1104 9 1.0158091071156647 1104 10 1.120972450890676 1104 11 1.0706991964046395 1104 12 0.9032310167007722 1104 13 1.0099992675576814 1104 14 1.2721855613927175 1104 15 1.3844284255479238 1104 16 1.2696221751837342 1104 17 1.2133227105530722 1104 18 1.4332038821567252 1104 19 1.3485092388849083 1104 20 1.3533565542908264 1104 21 1.3075114259894676 1104 22 1.2496239448506756 1104 23 1.5428877838975978 1104 24 1.4859961020989303 1104 25 1.1792209564453628 1105 1 0.60205104667493 1105 2 0.7299087071462864 1105 3 0.6926382527930208 1105 4 0.9660344799363116 1105 5 0.6066377419631763 1105 6 0.39923953473424945 1105 7 0.6726058406514994 1105 8 0.7732232972332653 1105 9 0.721635479364025 1105 10 0.8715787642349753 1105 11 0.7265837786552396 1105 12 0.7517799231016795 1105 13 0.8154057200256588 1105 14 0.8495178140472603 1105 15 0.9922886776252682 1105 16 1.0257726530390343 1105 17 0.8509079032146545 1105 18 0.9164684574981905 1105 19 0.9398848261314896 1105 20 0.9424009966178346 1105 21 0.9354085450550969 1105 22 0.6858013534096017 1105 23 0.9411351707213953 1105 24 0.9583072372076984 1105 25 0.9076913920773322 1106 1 0.639702201193721 1106 2 0.6696888325696193 1106 3 0.8297343640619133 1106 4 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1107 17 1.0662294391558405 1107 18 1.397266514578348 1107 19 1.3139202336113343 1107 20 1.2153746541078192 1107 21 1.2155040229764964 1107 22 1.1485601434920838 1107 23 1.1432712960072777 1107 24 1.163603514884855 1107 25 1.1759496890077017 1108 1 0.5104303791399936 1108 2 0.7089632906932088 1108 3 0.5420028022271843 1108 4 0.6645146061927534 1108 5 0.6437235037510476 1108 6 0.7173253640932344 1108 7 0.7003133833306805 1108 8 0.7679171467948862 1108 9 0.8894151889987754 1108 10 0.8679313029344868 1108 11 0.7693094094579394 1108 12 0.7905538345328651 1108 13 0.9175222254179971 1108 14 1.0965739887122012 1108 15 1.0408705554529263 1108 16 1.0639435643377126 1108 17 1.0903316757865724 1108 18 1.1898900294330375 1108 19 1.064676643783244 1108 20 1.0324631407333626 1108 21 0.922462972147926 1108 22 0.8345064513682914 1108 23 1.0675276340471385 1108 24 1.0906902122448727 1108 25 1.1261018009878967 1109 1 0.37154515304810687 1109 2 0.3310992567794244 1109 3 0.4996429238932727 1109 4 0.5154243219640762 1109 5 0.3874854597764852 1109 6 0.3810589503891677 1109 7 0.48453467706119824 1109 8 0.6285376891162642 1109 9 0.785676618565482 1109 10 0.8731335270504275 1109 11 0.4623405472779467 1109 12 0.6691868918115773 1109 13 0.737515406055337 1109 14 0.4768527215506353 1109 15 0.7667473060512222 1109 16 0.8603858349394713 1109 17 0.6743519065624639 1109 18 -0.9068896913765485 1109 19 0.5355579406233133 1109 20 0.7547592339175395 1109 21 0.8044723765705797 1109 22 0.8998704852820723 1109 23 0.4727644469938923 1109 24 0.9651748239052935 1109 25 0.989537052467881 1110 1 0.2289515481032076 1110 2 0.2631291006330372 1110 3 0.4254458828199823 1110 4 0.26815384945942355 1110 5 0.31036474432920613 1110 6 0.3665119959542338 1110 7 0.42777126094448903 1110 8 0.28728791607322535 1110 9 0.5313926825791881 1110 10 0.645936164290319 1110 11 0.595592036312965 1110 12 -0.05725694916856128 1110 13 0.3803140123422395 1110 14 0.526637827845434 1110 15 0.6113031266091795 1110 16 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0.3056952403190465 2211 9 0.3279648284405354 2211 10 0.3287913690866233 2211 11 0.20393266901336915 2211 12 0.2720352478480308 2211 13 0.15769949656188942 2211 14 0.35276610451685286 2211 15 0.4213887909721592 2211 16 0.302497154699239 2211 17 0.3288625667643785 2211 18 0.3707383508013393 2211 19 0.3811919423703287 2211 20 0.3452913232084285 2211 21 0.30754059361079555 2211 22 0.3249255322984652 2211 23 0.7068205617412602 2211 24 0.5635869640366806 2211 25 0.5614829745607537 2212 1 0.015757021038837138 2212 2 -0.07078561102334291 2212 3 -0.10399519206124097 2212 4 -0.20833862952705573 2212 5 -0.16524022705391417 2212 6 -0.29818655427845897 2212 7 -0.3282560706989699 2212 8 -0.25779902615846595 2212 9 -0.21985419678129858 2212 10 -0.1511994662613958 2212 11 -0.36421984798237617 2212 12 -0.045132386429138194 2212 13 -0.269747903731016 2212 14 -0.3137596989587337 2212 15 -0.4474620851993265 2212 16 -0.2248311588265821 2212 17 -0.3296458751398106 2212 18 -2.7994278018489496 2212 19 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-1.8181601507404466 2214 6 -1.8412895344593174 2214 7 -1.8090983443291755 2214 8 -1.5413514597250888 2214 9 -1.8302477909710575 2214 10 -1.890275842379161 2214 11 -1.5375912151997682 2214 12 -2.1014904700422576 2214 13 -1.9518646073136452 2214 14 -3.0486750747951774 2214 15 -3.3781766578525207 2214 16 -2.5184215402833487 2214 17 -3.034052449498141 2214 18 -2.3153024030521294 2214 19 -4.429681308485421 2214 20 -2.8338729519113386 2214 21 -2.5966876613082093 2214 22 -2.983869487979959 2214 23 -2.9527076452520973 2214 24 -3.0840481039021945 2214 25 -2.8341775754287326 2215 1 -1.1492123180719318 2215 2 -2.7380321470377496 2215 3 -1.7161504117420847 2215 4 -1.3626304400901645 2215 5 -1.2385857669939782 2215 6 -1.661476207822787 2215 7 -2.1933831406183586 2215 8 -2.205900878186597 2215 9 -1.8224839320372297 2215 10 -3.5426731498707156 2215 11 -2.3232669520942295 2215 12 -2.5866204914129334 2215 13 -1.9497687790275364 2215 14 -3.1484788486402238 2215 15 -3.0049460170276348 2215 16 -2.692622232614525 2215 17 -3.0076879760342194 2215 18 -2.017361423546312 2215 19 -2.461532481903056 2215 20 -2.70455573029642 2215 21 -2.3951662315409266 2215 22 -2.068636232447709 2215 23 -2.7360835722175594 2215 24 -2.5085624626341314 2215 25 -2.451994762574813 2216 1 -2.1937234646250836 2216 2 -2.337694501072093 2216 3 -2.7762736690457217 2216 4 -3.459440167534037 2216 5 -2.759579939540238 2216 6 -2.829045762053763 2216 7 -2.4987133428242068 2216 8 -3.0418854981405268 2216 9 -3.5583542886487223 2216 10 -3.1464805468917234 2216 11 -3.666375399155548 2216 12 -3.8297790057893053 2216 13 -4.5808854407596264 2216 14 -4.040108831862671 2216 15 -3.792211252295136 2216 16 -2.9926105246370867 2216 17 -3.221925109362715 2216 18 -3.2070322873948705 2216 19 -3.4165850211608593 2216 20 -3.0600183048038474 2216 21 -3.4518520452895807 2216 22 -4.098613309950331 2216 23 -4.172581876772082 2216 24 -4.235222970392591 2216 25 -4.124185127305076 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 76643.0 3 1.0 4 581.0 5 3756.0 6 4729.0 7 4261.0 8 3781.0 9 3617.0 10 3261.0 11 3166.0 12 2989.0 13 2962.0 14 2966.0 15 3090.0 16 3183.0 17 3345.0 18 3782.0 19 4644.0 20 5850.0 21 7553.0 22 9354.0 23 11661.0 24 13379.0 25 15288.0 26 17286.0 27 19006.0 28 21567.0 29 26137.0 30 31765.0 31 40368.0 32 50358.0 33 65443.0 34 86039.0 35 114376.0 36 159133.0 37 254449.0 38 720642.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.99278247815149 19.013016290850466 12.374981115814272 25.619220115183776 2 14.561456051145818 20.661192843096998 41.70783517630828 23.069515929448908 3 18.26780324750081 25.249863316931364 29.9885430696128 26.49379036595503 4 11.985827188151466 17.19617465206936 39.30149395705263 31.516504202726548 5 12.698525591694192 38.19509308469999 36.56565937195263 12.54072195165319 6 30.816874242042946 38.150370902448735 18.683228254028037 12.349526601480276 7 27.24173849277665 31.850794276873334 23.22666125945452 17.6808059708955 8 24.231272448477878 37.518049062337674 21.260920411899185 16.989758077285266 9 26.37753171782648 15.19550136582347 20.45057452362844 37.97639239272161 10 14.3331105581546 29.17899291174936 35.512141247241004 20.975755282855033 11 33.77560079153709 22.512030274849142 25.571625550959364 18.140743382654403 12 22.860571380953044 24.956208941542478 32.58154747850725 19.601672198997235 13 28.728973302156124 22.287480285083664 26.087966165064753 22.89558024769546 14 20.480317174233594 22.402763718307856 28.21460597124053 28.902313136218023 15 22.466874198905373 31.500415860826354 23.614950099478797 22.417759840789476 16 21.718253646384838 28.37163514262762 28.26196859781267 21.648142613174876 17 21.341165307015885 28.88478265217459 28.24393935016767 21.530112690641854 18 21.162690480228424 27.708676807077342 30.250248135163467 20.87838457753077 19 23.096184424130165 27.31077403777241 27.794679594519366 21.79836194357806 20 23.16210764212773 27.74442869738214 28.379212470339997 20.71425119015013 21 22.60616798534591 27.150847238218418 27.422639058573584 22.82034571786209 22 22.68375079790204 28.508032908285568 27.617104797523158 21.191111496289235 23 21.965806362723225 28.17097263086783 28.053027138019004 21.81019386838994 24 22.64267187094631 27.248692680287345 28.679147382555108 21.429488066211235 25 22.32228522920015 27.672798041308305 28.298583815237638 21.706332914253903 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 262.0 1 262.0 2 387.5 3 513.0 4 513.0 5 513.0 6 1057.5 7 1602.0 8 1602.0 9 1602.0 10 2428.0 11 3254.0 12 3254.0 13 3254.0 14 5821.0 15 8388.0 16 8388.0 17 8388.0 18 15623.0 19 22858.0 20 22858.0 21 22858.0 22 36259.5 23 49661.0 24 49661.0 25 49661.0 26 71353.0 27 93045.0 28 93045.0 29 93045.0 30 119638.0 31 146231.0 32 146231.0 33 146231.0 34 165714.5 35 185198.0 36 185198.0 37 185198.0 38 198387.5 39 211577.0 40 211577.0 41 211577.0 42 219825.0 43 228073.0 44 228073.0 45 228073.0 46 229973.5 47 231874.0 48 231874.0 49 231874.0 50 220297.5 51 208721.0 52 208721.0 53 208721.0 54 184871.5 55 161022.0 56 161022.0 57 161022.0 58 137525.5 59 114029.0 60 114029.0 61 114029.0 62 91846.0 63 69663.0 64 69663.0 65 69663.0 66 53438.0 67 37213.0 68 37213.0 69 37213.0 70 27224.0 71 17235.0 72 17235.0 73 17235.0 74 11925.0 75 6615.0 76 6615.0 77 6615.0 78 4473.0 79 2331.0 80 2331.0 81 2331.0 82 1532.0 83 733.0 84 733.0 85 733.0 86 481.0 87 229.0 88 229.0 89 229.0 90 143.5 91 58.0 92 58.0 93 58.0 94 38.0 95 18.0 96 18.0 97 18.0 98 13.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3655831918378637 2 0.03854675404671489 3 0.034825381537882186 4 0.034825381537882186 5 0.03921326852590881 6 0.018995662657026645 7 0.019217834150091286 8 0.02532755020936886 9 0.022106063559931593 10 0.021661720573802314 11 0.020495320235212958 12 0.021439549080737676 13 0.020995206094608396 14 0.02871566547860461 15 0.029437722831064682 16 0.02271703516585935 17 0.024383321363844142 18 0.11086357503925492 19 0.026271779054893578 20 0.023772349757916388 21 0.027993608126144532 22 0.020995206094608396 23 0.023716806884650225 24 0.019662177136220562 25 0.024105606997513344 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1800411.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.27351105370478 #Duplication Level Percentage of deduplicated Percentage of total 1 83.33101238435702 61.059558570615934 2 11.58935888501491 16.983860327329833 3 2.8294015382244915 6.219605546593847 4 0.9887190514908337 2.8978766539368843 5 0.4391899144617554 1.6090493525994547 6 0.22838418496737212 1.0040706661018857 7 0.13492718403075163 0.6920611957365777 8 0.09132170482338346 0.5353169558255483 9 0.06015942637045315 0.39672831538259434 >10 0.27343417964721567 3.4950808433866896 >50 0.018514350249718127 0.9154227100519857 >100 0.012766351008602602 1.9093264329582118 >500 0.0018232510400720307 0.8534001484624412 >1k 9.875943133723487E-4 1.4286422810181025 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 3085 0.17134976402610294 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2989 0.1660176481925516 No Hit GTATCAACGCAGAGTACTTTTTTTT 2984 0.1657399338262208 No Hit CTGTAGGACGTGGAATATGGCAAGA 2594 0.14407821325241849 No Hit CTTTAGGACGTGAAATATGGCGAGG 2527 0.14035684074358576 No Hit GTCCTACAGTGTGCATTTCTCATTT 2075 0.11525146202728154 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.554287326615978E-5 0.0 5 0.0 0.0 0.0 5.554287326615978E-5 0.0 6 5.554287326615978E-5 0.0 0.0 5.554287326615978E-5 5.554287326615978E-5 7 5.554287326615978E-5 0.0 0.0 5.554287326615978E-5 5.554287326615978E-5 8 5.554287326615978E-5 0.0 0.0 5.554287326615978E-5 5.554287326615978E-5 9 5.554287326615978E-5 0.0 0.0 1.6662861979847935E-4 5.554287326615978E-5 10 5.554287326615978E-5 0.0 0.0 1.6662861979847935E-4 5.554287326615978E-5 11 1.1108574653231956E-4 0.0 0.0 1.6662861979847935E-4 5.554287326615978E-5 12 1.1108574653231956E-4 0.0 0.0 1.6662861979847935E-4 1.1108574653231956E-4 13 1.1108574653231956E-4 0.0 0.0 1.6662861979847935E-4 1.6662861979847935E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCGC 55 5.9432386E-7 17.263735 10 GCGTGCG 80 2.0116277E-6 13.0557 9 TAGGACC 650 0.0 13.004535 4 GGCGAGG 655 0.0 11.596692 19 AGACCCG 85 5.338404E-5 11.173447 5 AATCCCG 155 3.1650416E-10 11.02621 19 AAGGCGT 150 2.1518645E-9 10.760764 6 AGGACCT 1175 0.0 10.750282 5 TGGCGAG 1500 0.0 10.706387 18 ACTAGAC 125 2.2477252E-7 10.637417 3 GTCCTAA 955 0.0 10.572496 1 GGACCTG 1170 0.0 10.5497675 6 CTAGGAC 235 0.0 10.508086 3 GTTATAA 210 0.0 10.4323845 1 TGTAGGA 1755 0.0 10.390639 2 GGTATCA 805 0.0 10.176013 1 GTCCTAG 200 2.3646862E-11 10.001482 1 TAAAGTG 1095 0.0 9.974469 5 GCGCCAC 105 4.0910883E-5 9.955498 13 GTCCTAC 1535 0.0 9.928569 1 >>END_MODULE