FastQCFastQC Report
Thu 26 May 2016
SRR1512976_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512976_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800411
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32810.18223616718627023No Hit
GTCCTACAGTGGACATTTCTAAATT29430.16346267602230824No Hit
GTCCTAAAGTGTGTATTTCTCATTT27700.1538537589472626No Hit
CTGTAGGACGTGGAATATGGCAAGA27460.15252072998887478No Hit
CTTTAGGACGTGAAATATGGCGAGG26700.1482994716206466No Hit
TATCAACGCAGAGTACTTTTTTTTT23210.12891500885075685No Hit
GGTATCAACGCAGAGTACTTTTTTT20550.11414060456195836No Hit
GTCCTACAGTGTGCATTTCTCATTT20550.11414060456195836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6200.013.1956244
TTAGACC657.9320883E-411.7084624
GGCGAGG6800.011.58548519
CGCAGTT600.005910846411.074612
GGTATCA8400.010.9786711
GACGTGG7650.010.9337677
TGTAGGA17250.010.9103022
GTAGGAC16600.010.8797333
AGTGCGT700.001486546710.8615618
GTCGAGG1053.502988E-610.84768419
GCACCGT1158.690149E-710.7512746
ATGTCGA1158.9075183E-710.73066917
GGACGTG15800.010.5942446
CGTGGAA8200.010.5460939
AGGACCT11400.010.5145055
TGGCGAG15550.010.49917618
CTGTAGG17750.010.4982041
TAATACT2450.010.4838514
AGGACGT16000.010.46443755
CACCGTG1659.2040864E-1010.3690067
Produced by FastQC (version 0.11.5)