##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512976_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1800411 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.52412310300259 31.0 31.0 34.0 27.0 34.0 2 30.673039100516494 31.0 31.0 34.0 27.0 34.0 3 30.76470205969637 31.0 31.0 34.0 27.0 34.0 4 34.23511798139425 37.0 35.0 37.0 30.0 37.0 5 33.882967278027074 37.0 35.0 37.0 30.0 37.0 6 33.73989550163824 37.0 35.0 37.0 29.0 37.0 7 33.62274780591765 37.0 35.0 37.0 28.0 37.0 8 33.51209251665314 37.0 35.0 37.0 28.0 37.0 9 34.98423526628087 39.0 35.0 39.0 28.0 39.0 10 34.872278052067 39.0 35.0 39.0 27.0 39.0 11 34.891706949135504 39.0 35.0 39.0 27.0 39.0 12 34.74451278069285 39.0 35.0 39.0 27.0 39.0 13 34.724680086935706 39.0 35.0 39.0 27.0 39.0 14 35.80292777593561 40.0 36.0 41.0 27.0 41.0 15 35.81540103898499 40.0 36.0 41.0 27.0 41.0 16 35.79326442684476 40.0 36.0 41.0 27.0 41.0 17 35.77660545286604 40.0 36.0 41.0 27.0 41.0 18 35.70025899641804 40.0 36.0 41.0 27.0 41.0 19 35.69315895092843 40.0 36.0 41.0 27.0 41.0 20 35.657980316716575 40.0 36.0 41.0 27.0 41.0 21 35.61047727435569 40.0 36.0 41.0 27.0 41.0 22 35.56455609302542 40.0 36.0 41.0 27.0 41.0 23 35.45844198907916 40.0 36.0 41.0 26.0 41.0 24 35.448044363203735 40.0 36.0 41.0 26.0 41.0 25 35.356151456528536 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4262232362591547 1101 2 0.5057515363795524 1101 3 0.5262867848445509 1101 4 0.3214033231126976 1101 5 0.5610252896948253 1101 6 0.468380726517303 1101 7 0.5359556180633902 1101 8 0.49353625425423076 1101 9 0.3555452873325464 1101 10 0.6999767898426015 1101 11 0.5668936653772505 1101 12 0.7334495733212165 1101 13 0.7612556503468255 1101 14 0.6894888502582006 1101 15 0.6874251328202448 1101 16 0.8154078292206748 1101 17 0.749386385973807 1101 18 0.22435895247246407 1101 19 0.5220041692623383 1101 20 0.4855356625504683 1101 21 0.66454357123596 1101 22 0.6197204525619284 1101 23 0.48963201536297163 1101 24 0.4624038146593463 1101 25 0.4991687971801042 1102 1 0.646110202433249 1102 2 0.5852716505531319 1102 3 0.5093552493486584 1102 4 0.38969314759064133 1102 5 0.535378355632055 1102 6 0.5028706619973349 1102 7 0.5906414920561929 1102 8 0.5639048902169321 1102 9 0.7223774660459128 1102 10 0.7442425902965155 1102 11 0.8016197280736108 1102 12 0.8531026422317112 1102 13 0.915459359005979 1102 14 0.9574139535793407 1102 15 0.9134680073779649 1102 16 0.9374568584479235 1102 17 1.0134301326908215 1102 18 1.0427144366695345 1102 19 1.115658467124554 1102 20 1.1040266783187107 1102 21 1.0255679619633256 1102 22 1.1428413457323217 1102 23 1.1188862696172208 1102 24 0.9924152617499971 1102 25 1.1434083337463719 1103 1 0.5434012810687783 1103 2 0.488430387479454 1103 3 0.592915292262532 1103 4 0.4958837936458025 1103 5 0.6467128359086871 1103 6 0.7585634377526418 1103 7 0.7674656522050043 1103 8 0.7943199695116334 1103 9 0.7700116491673299 1103 10 0.8099474063921619 1103 11 0.7546602413017212 1103 12 0.7796348366117414 1103 13 0.8506295291943857 1103 14 1.0391128117242658 1103 15 0.8864495160551513 1103 16 0.9922068678618245 1103 17 1.0569297593066551 1103 18 1.0482060789511678 1103 19 0.9976948489003803 1103 20 1.0937176852061725 1103 21 0.7497870775320976 1103 22 1.066847511531094 1103 23 1.0185168534583369 1103 24 1.0152082293058626 1103 25 1.100167707389545 1104 1 0.5579385105800618 1104 2 0.562120473030177 1104 3 0.5065610732174299 1104 4 0.5069253836347585 1104 5 0.5791189521546869 1104 6 0.628759957319069 1104 7 0.6136782486029233 1104 8 0.5572748303167501 1104 9 0.6193898469517549 1104 10 0.7813014785616517 1104 11 0.704427054671207 1104 12 0.6628428072919164 1104 13 0.7522954442035186 1104 14 0.9099244809050973 1104 15 0.9279565634925149 1104 16 0.8271014025339696 1104 17 0.9912590820718563 1104 18 0.9851964421831951 1104 19 1.0514945209357833 1104 20 1.146439117468006 1104 21 0.9229482380913367 1104 22 1.0328363040580584 1104 23 0.9125141336676208 1104 24 0.9784392162581241 1104 25 1.0700374719220207 1105 1 0.5346431382458299 1105 2 0.5637818794261804 1105 3 0.49888927332651534 1105 4 0.47457481708224236 1105 5 0.46184060011925965 1105 6 0.5069306771193709 1105 7 0.6547918810364592 1105 8 0.755820933069856 1105 9 0.7174120400787771 1105 10 0.7028122800559373 1105 11 0.7716957801956497 1105 12 0.5646492583482612 1105 13 0.7317147087537634 1105 14 0.8579883758424174 1105 15 0.7667699499500742 1105 16 0.8296317436888927 1105 17 0.5470969630003495 1105 18 0.8496218375073354 1105 19 0.9290590254214237 1105 20 0.9093955974038863 1105 21 0.8239277110761307 1105 22 0.9292730833686278 1105 23 0.8566109649966407 1105 24 0.9349622851819888 1105 25 0.9545358091432092 1106 1 0.38825617878186947 1106 2 0.4233435094074771 1106 3 0.4805802871735736 1106 4 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1107 17 0.5152299250731645 1107 18 0.64880295249786 1107 19 0.8463594441990026 1107 20 0.7443740463888489 1107 21 0.7477642125961381 1107 22 0.5957924547967224 1107 23 0.6358387400580696 1107 24 0.699706921148497 1107 25 0.7300119553488429 1108 1 0.5172436383723849 1108 2 0.5689513832648849 1108 3 0.46553045352188605 1108 4 0.6161151201192681 1108 5 0.6729147163573757 1108 6 0.8064812100975232 1108 7 0.7354631442941582 1108 8 0.8148212992576731 1108 9 0.8139073556703664 1108 10 0.9062378222640035 1108 11 0.819085197054406 1108 12 0.9559057062813352 1108 13 0.9692461139659656 1108 14 1.025785009597307 1108 15 0.9344405930327895 1108 16 0.9412350332865387 1108 17 0.9275223501333301 1108 18 0.8959417753368939 1108 19 0.8898581247072173 1108 20 0.9073354932089757 1108 21 1.0566544212616051 1108 22 1.041561110330882 1108 23 1.0354984134548602 1108 24 0.9380834690279514 1108 25 0.9309928536577843 1109 1 0.28273493261460203 1109 2 0.32261100948902666 1109 3 0.382938180558444 1109 4 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2216 15 -2.187704293876422 2216 16 -1.9392329338882348 2216 17 -2.118192264466316 2216 18 -1.891933022009603 2216 19 -1.9671957232489845 2216 20 -2.5089733813771815 2216 21 -2.2285203723840965 2216 22 -2.4074094326316597 2216 23 -1.765546191154975 2216 24 -2.147889365274885 2216 25 -1.9274933300634203 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45613.0 3 1.0 4 783.0 5 6767.0 6 7803.0 7 6140.0 8 4906.0 9 4330.0 10 3793.0 11 3299.0 12 3066.0 13 2946.0 14 2781.0 15 2816.0 16 2922.0 17 3054.0 18 3416.0 19 4048.0 20 5067.0 21 6591.0 22 8335.0 23 10158.0 24 12013.0 25 13589.0 26 15614.0 27 17162.0 28 19467.0 29 23591.0 30 29398.0 31 37659.0 32 48643.0 33 62574.0 34 82178.0 35 108236.0 36 149486.0 37 233904.0 38 808262.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.451758128324435 19.585633470391947 13.253291991548577 27.709316409735035 2 13.352188308323276 20.90713832245895 40.69825245263715 25.04242091658062 3 16.721070911030868 25.12654055101863 30.54713618168296 27.605252356267542 4 11.080696229783163 17.693923573397306 38.64604208068901 32.57933811613052 5 12.306261710449599 38.437732228880336 36.583347275171874 12.67265878549819 6 30.94971936610147 38.36221200769555 18.30504574024035 12.383022885962635 7 26.965515517044995 31.918023997177457 23.212421414661115 17.904039071116433 8 24.217632406441005 37.8257433534184 20.94822229854625 17.008401941594332 9 25.99581702949083 14.912334317583479 20.30196512931758 38.78988352360811 10 13.98124713658543 29.33381965047438 35.539645225714914 21.145287987225277 11 33.66512177672711 22.42715836529019 25.518734197464482 18.388985660518212 12 22.626983068924144 24.975836555125984 32.54410523041372 19.85307514553615 13 28.596765154002064 22.18467361315934 26.089784500978432 23.128776731860164 14 20.274441471501312 22.349527387212543 28.24484214116339 29.13118900012276 15 22.32582708769482 31.45757733473382 23.610763179718305 22.60583239785305 16 21.621926397980634 28.412625932690727 28.155816568231412 21.80963110109723 17 21.19034510574728 28.895960497755834 28.217793454329698 21.69590094216719 18 21.11479918862295 27.864814185323674 30.062997251715736 20.957389374337644 19 22.962519433472952 27.218137574824773 27.819124148162633 22.000218843539646 20 23.023924805690452 27.728401548810815 28.35466901139723 20.8930046341015 21 22.402533203584966 27.03026202949554 27.517818183433977 23.04938658348552 22 22.627964263596244 28.35345380622693 27.6481680510774 21.370413879099427 23 21.767918547487213 28.04409659794347 28.08830872506333 22.099676129505987 24 22.527702023428 27.299115201537425 28.656306063618842 21.516876711415733 25 22.23413963817096 27.577202915206072 28.320612679846676 21.868044766776293 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 131.0 1 131.0 2 248.5 3 366.0 4 366.0 5 366.0 6 938.0 7 1510.0 8 1510.0 9 1510.0 10 2331.0 11 3152.0 12 3152.0 13 3152.0 14 5629.0 15 8106.0 16 8106.0 17 8106.0 18 15295.0 19 22484.0 20 22484.0 21 22484.0 22 35867.5 23 49251.0 24 49251.0 25 49251.0 26 71166.5 27 93082.0 28 93082.0 29 93082.0 30 119389.0 31 145696.0 32 145696.0 33 145696.0 34 165357.5 35 185019.0 36 185019.0 37 185019.0 38 198146.5 39 211274.0 40 211274.0 41 211274.0 42 220139.5 43 229005.0 44 229005.0 45 229005.0 46 231201.5 47 233398.0 48 233398.0 49 233398.0 50 221765.5 51 210133.0 52 210133.0 53 210133.0 54 185948.5 55 161764.0 56 161764.0 57 161764.0 58 137689.0 59 113614.0 60 113614.0 61 113614.0 62 91830.0 63 70046.0 64 70046.0 65 70046.0 66 53229.0 67 36412.0 68 36412.0 69 36412.0 70 26503.0 71 16594.0 72 16594.0 73 16594.0 74 11423.5 75 6253.0 76 6253.0 77 6253.0 78 4244.5 79 2236.0 80 2236.0 81 2236.0 82 1439.0 83 642.0 84 642.0 85 642.0 86 422.0 87 202.0 88 202.0 89 202.0 90 118.5 91 35.0 92 35.0 93 35.0 94 20.0 95 5.0 96 5.0 97 5.0 98 3.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07909305153101154 2 1.1108574653231956E-4 3 0.0 4 0.027104922153885973 5 0.07053944904802292 6 0.08042608048939937 7 0.034214409931954425 8 0.059153160028460175 9 0.040712926104095125 10 0.10325420140179103 11 0.0037213725088327053 12 0.009497831328513322 13 0.004332344114760463 14 0.005554287326615978 15 0.004887772847422061 16 0.013496918203676829 17 0.02288366378565783 18 8.331430989923968E-4 19 0.002110629184114072 20 0.0034436581425019066 21 1.6662861979847935E-4 22 3.332572395969587E-4 23 0.0 24 5.554287326615978E-5 25 0.0032214866494372674 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1800411.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.53273687563774 #Duplication Level Percentage of deduplicated Percentage of total 1 82.70611001377522 59.98900515636705 2 11.999446287554864 17.407053604571303 3 2.9772370545005877 6.478414556714696 4 1.0150523024921068 2.9449808628668084 5 0.4617274874006867 1.6745179175941673 6 0.2191050952477994 0.9535375333032108 7 0.1359700245439591 0.6903594609254693 8 0.09534333546593847 0.5532410451357259 9 0.06544039571398874 0.42719139030243214 >10 0.289577209418184 3.617937183220403 >50 0.018903916649704523 0.9280300977083683 >100 0.013176584855273453 1.905623950542797 >500 0.0018380793990246309 0.8658083357173604 >1k 0.001072212982764367 1.5642989050302096 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3281 0.18223616718627023 No Hit GTCCTACAGTGGACATTTCTAAATT 2943 0.16346267602230824 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2770 0.1538537589472626 No Hit CTGTAGGACGTGGAATATGGCAAGA 2746 0.15252072998887478 No Hit CTTTAGGACGTGAAATATGGCGAGG 2670 0.1482994716206466 No Hit TATCAACGCAGAGTACTTTTTTTTT 2321 0.12891500885075685 No Hit GGTATCAACGCAGAGTACTTTTTTT 2055 0.11414060456195836 No Hit GTCCTACAGTGTGCATTTCTCATTT 2055 0.11414060456195836 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.554287326615978E-5 2 0.0 0.0 0.0 0.0 5.554287326615978E-5 3 0.0 0.0 0.0 0.0 5.554287326615978E-5 4 0.0 0.0 0.0 0.0 5.554287326615978E-5 5 0.0 0.0 0.0 0.0 5.554287326615978E-5 6 1.1108574653231956E-4 0.0 0.0 0.0 5.554287326615978E-5 7 1.1108574653231956E-4 0.0 0.0 0.0 5.554287326615978E-5 8 1.1108574653231956E-4 0.0 0.0 5.554287326615978E-5 5.554287326615978E-5 9 1.1108574653231956E-4 0.0 0.0 5.554287326615978E-5 5.554287326615978E-5 10 1.1108574653231956E-4 0.0 0.0 5.554287326615978E-5 5.554287326615978E-5 11 1.1108574653231956E-4 0.0 0.0 5.554287326615978E-5 5.554287326615978E-5 12 1.6662861979847935E-4 0.0 0.0 1.1108574653231956E-4 1.1108574653231956E-4 13 1.6662861979847935E-4 0.0 0.0 1.6662861979847935E-4 1.1108574653231956E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 620 0.0 13.195624 4 TTAGACC 65 7.9320883E-4 11.708462 4 GGCGAGG 680 0.0 11.585485 19 CGCAGTT 60 0.0059108464 11.0746 12 GGTATCA 840 0.0 10.978671 1 GACGTGG 765 0.0 10.933767 7 TGTAGGA 1725 0.0 10.910302 2 GTAGGAC 1660 0.0 10.879733 3 AGTGCGT 70 0.0014865467 10.861561 8 GTCGAGG 105 3.502988E-6 10.847684 19 GCACCGT 115 8.690149E-7 10.751274 6 ATGTCGA 115 8.9075183E-7 10.730669 17 GGACGTG 1580 0.0 10.594244 6 CGTGGAA 820 0.0 10.546093 9 AGGACCT 1140 0.0 10.514505 5 TGGCGAG 1555 0.0 10.499176 18 CTGTAGG 1775 0.0 10.498204 1 TAATACT 245 0.0 10.483851 4 AGGACGT 1600 0.0 10.4644375 5 CACCGTG 165 9.2040864E-10 10.369006 7 >>END_MODULE