FastQCFastQC Report
Thu 26 May 2016
SRR1512975_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512975_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2509037
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT58560.23339631898612895No Hit
GTCCTAAAGTGTGTATTTCTCATTT57310.2284143278875521No Hit
CTGTAGGACGTGGAATATGGCAAGA53530.21334878680545566No Hit
CTTTAGGACGTGAAATATGGCGAGG46160.18397496728824644No Hit
GTCCTACAGTGTGCATTTCTCATTT36940.14722780094514348No Hit
GTATCAACGCAGAGTACTTTTTTTT36750.1464705382981598No Hit
CTGTAGGACCTGGAATATGGCGAGA26090.10398411820949631No Hit
CTGAAGGACCTGGAATATGGCGAGA26010.1036652707791874No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC350.00217838116.2771038
TAGGACC10700.012.6020674
GCGGGCC550.003061347912.09512313
TGTACCG1353.7471182E-1011.9575925
GTACCGT1052.7423994E-711.7552186
CGTGCGC855.353326E-511.17078410
GGCGAGG14500.011.00129619
GGTATCA11150.010.8531951
TGTAGGA33900.010.7004212
AGGACCT21000.010.580965
CACCGTG1355.739639E-810.5495547
TCCAACG3000.010.45364318
CCAACGA2001.8189894E-1210.44468319
CTGTAGG34200.010.3922961
GGACCTG20250.010.315126
GTCCTAC29950.010.2762451
AATGTCC8450.010.2253598
GACGTGA17200.010.21221357
GGACGTG31350.010.2063156
AAATGTC8400.010.1727847
Produced by FastQC (version 0.11.5)