##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512975_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2509037
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.10260470451412	31.0	30.0	34.0	23.0	34.0
2	29.233053557998545	31.0	30.0	34.0	23.0	34.0
3	29.505345676448773	31.0	30.0	34.0	25.0	34.0
4	32.945386616458826	35.0	35.0	37.0	26.0	37.0
5	33.134166614521824	37.0	35.0	37.0	27.0	37.0
6	33.149356506101746	37.0	35.0	37.0	26.0	37.0
7	32.95387792208724	37.0	35.0	37.0	26.0	37.0
8	33.009666258409105	37.0	35.0	37.0	26.0	37.0
9	34.35824461735718	39.0	35.0	39.0	26.0	39.0
10	34.20681082024697	39.0	35.0	39.0	25.0	39.0
11	34.30780175820444	39.0	35.0	39.0	26.0	39.0
12	34.1929584936372	39.0	35.0	39.0	25.0	39.0
13	34.21118859546511	39.0	35.0	39.0	25.0	39.0
14	35.237571227526736	40.0	36.0	41.0	25.0	41.0
15	35.17581167595376	40.0	35.0	41.0	25.0	41.0
16	35.07879078706293	40.0	35.0	41.0	24.0	41.0
17	35.041013743519926	40.0	35.0	41.0	24.0	41.0
18	34.72635875836028	39.0	34.0	41.0	19.0	41.0
19	34.96582354106376	40.0	35.0	41.0	22.0	41.0
20	35.03996911962638	40.0	35.0	41.0	24.0	41.0
21	34.983309134141905	40.0	35.0	41.0	23.0	41.0
22	34.91486853322609	40.0	35.0	41.0	22.0	41.0
23	34.64428703123948	39.0	35.0	41.0	18.0	41.0
24	34.653889520162515	39.0	35.0	41.0	18.0	41.0
25	34.586755396592395	39.0	35.0	41.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5108354489426716
1101	2	0.8587660675229891
1101	3	0.8523793828887349
1101	4	0.80752571812247
1101	5	0.7881700907554077
1101	6	0.8887460517401067
1101	7	0.904559359693792
1101	8	0.7108482311748645
1101	9	0.6909792035666129
1101	10	0.870555302782769
1101	11	0.8843530394953447
1101	12	1.03943913320051
1101	13	0.9033746731968151
1101	14	0.767076609405521
1101	15	0.7009056277418821
1101	16	1.0948157263804177
1101	17	1.0338009594166238
1101	18	1.244073309341303
1101	19	0.8828624209616152
1101	20	0.9010957517063218
1101	21	1.0356147347423175
1101	22	1.0102261152601884
1101	23	1.2765415453214146
1101	24	0.7562483284525428
1101	25	1.1578104436101881
1102	1	0.6694585707500096
1102	2	0.8044871845150823
1102	3	0.8201390366811516
1102	4	1.0004992467152363
1102	5	0.9073982148312112
1102	6	0.9127584501650219
1102	7	1.0614908707957937
1102	8	1.0397654441910902
1102	9	1.0820818117803555
1102	10	1.1965868715324746
1102	11	1.1878138571711006
1102	12	1.1455879891823528
1102	13	1.0516899520211638
1102	14	1.1502069934138106
1102	15	1.3337094601697217
1102	16	1.2790998830239175
1102	17	1.0945069626450774
1102	18	1.4770712158034698
1102	19	1.4728809097610949
1102	20	1.3903863605573292
1102	21	1.2269612277594888
1102	22	1.4768943584088987
1102	23	1.7497143685294105
1102	24	1.4758790314710666
1102	25	1.319775934721477
1103	1	0.5037516170039993
1103	2	0.7189533253371962
1103	3	0.6458692419363139
1103	4	0.8516412029936973
1103	5	0.452140501726781
1103	6	0.8183559025311666
1103	7	0.6122591072278567
1103	8	0.7190064808408465
1103	9	1.073358722694401
1103	10	0.9525958418741496
1103	11	0.9964527827187766
1103	12	1.1884033039277995
1103	13	1.0673298989839282
1103	14	1.1941111961307485
1103	15	1.2960729108215219
1103	16	1.3383075561107134
1103	17	1.2271557569890703
1103	18	1.547106510333684
1103	19	1.5212072494417583
1103	20	1.299429812647368
1103	21	1.2192486782035417
1103	22	1.3349811382968326
1103	23	1.5631853731695031
1103	24	1.419982634300844
1103	25	1.3825061803715855
1104	1	0.5915602408706988
1104	2	0.6516106638164629
1104	3	0.6883259912895241
1104	4	0.9228226831331483
1104	5	0.8023341619808022
1104	6	0.7596195276293258
1104	7	0.9512086094193535
1104	8	0.8967750315847383
1104	9	0.9856558371974558
1104	10	0.966310022464107
1104	11	1.0087356268402132
1104	12	0.9465615994252801
1104	13	0.9525872193243359
1104	14	1.116096051473349
1104	15	1.2023745308107152
1104	16	1.0736794478432188
1104	17	1.0864715007875816
1104	18	1.3455266243505903
1104	19	1.1678536882723307
1104	20	1.172430293628004
1104	21	1.1878529790042762
1104	22	1.154188083330503
1104	23	1.3802971081083442
1104	24	1.2647402247898967
1104	25	1.046832633403767
1105	1	0.5309608237271597
1105	2	0.6409784236981686
1105	3	0.7082993792615078
1105	4	0.8216830241384372
1105	5	0.5803714199251786
1105	6	0.3558767691290612
1105	7	0.773333780993994
1105	8	0.8359667409654747
1105	9	0.8003199984875593
1105	10	0.9478446125747766
1105	11	0.9425505019471387
1105	12	0.9261205945780873
1105	13	0.879182835854742
1105	14	1.0527550318171421
1105	15	1.1285512952390135
1105	16	1.191373158651814
1105	17	1.0438995775822946
1105	18	1.1631364265949315
1105	19	1.0914966185130055
1105	20	1.2157204266218358
1105	21	1.1273533017082684
1105	22	0.9069479208291469
1105	23	1.130408728325527
1105	24	1.2183548410705285
1105	25	1.2484252383986032
1106	1	0.5656223041888069
1106	2	0.6025482949483418
1106	3	0.6999978658863668
1106	4	0.6533812149721427
1106	5	0.6895639369466195
1106	6	0.5965639188854368
1106	7	0.3805002630152714
1106	8	0.6740905914134672
1106	9	0.7812090660462658
1106	10	0.8232331745994941
1106	11	0.8810833509000418
1106	12	0.9170980230235202
1106	13	0.9047545194980273
1106	14	1.0262132876173382
1106	15	1.1225260292958694
1106	16	1.0213070896625567
1106	17	0.9994285866281203
1106	18	1.2465694325860497
1106	19	0.9576054051238856
1106	20	0.9370515406251272
1106	21	0.9763222006122234
1106	22	1.1176407712878955
1106	23	1.245906571739063
1106	24	1.0574659617425723
1106	25	1.05912171902159
1107	1	0.34482358789846757
1107	2	0.4208401751572737
1107	3	0.564112613810984
1107	4	0.5999841327917963
1107	5	0.5838481541948255
1107	6	0.35774349087305524
1107	7	0.5967139744753212
1107	8	0.539585654698385
1107	9	0.6437807425645303
1107	10	0.47802956580346034
1107	11	0.5364944163463079
1107	12	0.6703287469113235
1107	13	0.6139477800223148
1107	14	0.8234441424959229
1107	15	0.9239990206341062
1107	16	0.7503569152810812
1107	17	0.7940242344594068
1107	18	1.1615845783951642
1107	19	0.9767136853786624
1107	20	0.8566962281302395
1107	21	0.9489876992777582
1107	22	0.8211270909381483
1107	23	1.0370944943890095
1107	24	0.8696513791938187
1107	25	0.8895931220965991
1108	1	0.6380853743932242
1108	2	0.6676147624148818
1108	3	0.5591400549287613
1108	4	0.6849862795185615
1108	5	0.6396461978594132
1108	6	0.7355383281157941
1108	7	0.8136979118134136
1108	8	0.8562223529522228
1108	9	0.9798974344505424
1108	10	0.9580648139313084
1108	11	0.8961638043442548
1108	12	0.854917274867816
1108	13	0.7482315049389783
1108	14	0.920672179551481
1108	15	0.9087451360234837
1108	16	0.8458172072680838
1108	17	1.0058623310605057
1108	18	1.1798752049981687
1108	19	1.1926470612713231
1108	20	1.0678697560143746
1108	21	1.175366870721632
1108	22	0.8849437620805105
1108	23	1.1173697256646449
1108	24	1.1344362160667032
1108	25	1.2167775653225092
1109	1	0.02711859070970135
1109	2	0.05144621857825982
1109	3	0.2462250404313302
1109	4	0.3200169278051419
1109	5	0.21689386143452083
1109	6	0.23099225498390297
1109	7	0.26764051461761795
1109	8	0.38556199497581645
1109	9	0.521350411714046
1109	10	0.6712551919305909
1109	11	0.2929452959299539
1109	12	0.48374158330730666
1109	13	0.5113826072246752
1109	14	0.37479325869948354
1109	15	0.5078444951977161
1109	16	0.6528464521487365
1109	17	0.4413612437291832
1109	18	-1.1448917562111944
1109	19	0.241757020488933
1109	20	0.48789517428257767
1109	21	0.6597774855106593
1109	22	0.6581396337345069
1109	23	0.3122140649428431
1109	24	0.7606380975374734
1109	25	0.7259357651737588
1110	1	0.28206329276067166
1110	2	0.30986331950992607
1110	3	0.36143745457531296
1110	4	0.3410577911130446
1110	5	0.4125118358496138
1110	6	0.4484696685863696
1110	7	0.4390987256892984
1110	8	0.3991274974176875
1110	9	0.6548588153399564
1110	10	0.7998341316103605
1110	11	0.5245293535979982
1110	12	0.06787304911224368
1110	13	0.32945418098088197
1110	14	0.49067344185898065
1110	15	0.5771386435135923
1110	16	0.6721775623139408
1110	17	0.31310035226226773
1110	18	0.7463938109107673
1110	19	0.5870608506684434
1110	20	0.6207206337555178
1110	21	0.6133930092355087
1110	22	0.578267155263049
1110	23	0.6095337956439764
1110	24	0.7808688225942078
1110	25	0.7475236890386441
1111	1	0.03544780463985475
1111	2	0.34681781110512944
1111	3	0.23552467567064994
1111	4	0.17877594426710175
1111	5	0.0556428984487809
1111	6	0.2706946801341772
1111	7	0.16660208961595657
1111	8	0.05455044843490242
1111	9	0.2579119553395586
1111	10	0.3028194909982247
1111	11	0.1498125259314591
1111	12	0.24174487848585358
1111	13	0.23909609851948943
1111	14	0.22809737695025945
1111	15	0.35684362212504084
1111	16	0.2709384323157451
1111	17	0.2884864507098399
1111	18	0.5170016272704956
1111	19	0.2266216323044148
1111	20	0.17509624880180752
1111	21	0.353998616449374
1111	22	0.17282713357325719
1111	23	0.5240960835157651
1111	24	0.582840485277714
1111	25	0.20562573149441477
1112	1	-0.3688726527215813
1112	2	-0.010917737298562713
1112	3	-0.04654899266573054
1112	4	0.030402245887664492
1112	5	-0.06502834798483548
1112	6	-0.16196590788511855
1112	7	-0.43687220393028525
1112	8	-0.09391185734062901
1112	9	0.0967235657065757
1112	10	0.00396441637165168
1112	11	0.07255203098674201
1112	12	0.014022397832206934
1112	13	-0.26064986088506714
1112	14	-0.08393933645660212
1112	15	-0.05135641557605908
1112	16	0.03295123890715246
1112	17	0.0898910129353041
1112	18	0.10527594791440009
1112	19	-0.006092561962269372
1112	20	-0.09214562140823546
1112	21	-0.02972589849263585
1112	22	-0.08431275713283526
1112	23	0.05127130918235423
1112	24	-0.1908100805332822
1112	25	0.11584459421725768
1113	1	-0.09944585815441798
1113	2	-0.026009467768801642
1113	3	-0.18850193936408033
1113	4	0.11504608994292909
1113	5	-0.07140295562953014
1113	6	0.06910423204271865
1113	7	-0.03664174835810741
1113	8	0.1619936840363536
1113	9	-0.07746071661323839
1113	10	-0.20407195899869635
1113	11	-0.2602861279501596
1113	12	0.023426604467779555
1113	13	-0.11181944638185115
1113	14	-0.012326558141019461
1113	15	-0.41978634898566014
1113	16	-0.37330878632092634
1113	17	-0.3415573135043033
1113	18	-0.4325848869590203
1113	19	-0.3999703524518736
1113	20	-0.4979457328811492
1113	21	-0.4096009901644351
1113	22	-0.259549226375114
1113	23	-0.5765214098765767
1113	24	-0.10433819810786105
1113	25	-0.1131306763979012
1114	1	-1.4757905143747614
1114	2	-1.2434602461720061
1114	3	-1.1225931454724964
1114	4	-1.3263119610426628
1114	5	-1.3528922159110444
1114	6	-1.6846045024453637
1114	7	-1.8492038213954345
1114	8	-1.4224227779780065
1114	9	-1.5899668344494984
1114	10	-1.6318180887156615
1114	11	-2.315965175175947
1114	12	-1.8499648283881527
1114	13	-1.7319520176117251
1114	14	-1.9881276578302334
1114	15	-1.9901410458466913
1114	16	-2.688200606748488
1114	17	-4.6190007026914195
1114	18	-3.526585220198175
1114	19	-2.617340805729569
1114	20	-1.7874702875359887
1114	21	-2.659444063498782
1114	22	-2.738820041144848
1114	23	-2.770152369243135
1114	24	-2.333036174766633
1114	25	-2.16837819340396
1115	1	-1.0477732106265059
1115	2	-0.7134412289900389
1115	3	-0.8381396130938583
1115	4	-0.8628686400639083
1115	5	-0.7336101212709067
1115	6	-0.8898585157358241
1115	7	-1.6798192375355931
1115	8	-1.2013126499805082
1115	9	-1.0561946537287525
1115	10	-0.7932091200832261
1115	11	-1.617978369918717
1115	12	-1.2056087680544323
1115	13	-0.9635820916313378
1115	14	-1.039965655885382
1115	15	-2.2117715961945947
1115	16	-1.0899004358994162
1115	17	-1.1184260193598519
1115	18	-1.15835801295583
1115	19	-1.2113325648009265
1115	20	-0.9183487290078247
1115	21	-1.6623081558058104
1115	22	-1.7847204498884395
1115	23	-1.2160831551488513
1115	24	-1.1122107203224942
1115	25	-1.4245739436569522
1116	1	-1.0421923514272287
1116	2	-1.9590391982722153
1116	3	-1.515395653836741
1116	4	-1.1349129334897796
1116	5	-1.6958454371246425
1116	6	-1.4184596660474966
1116	7	-1.1892819969983535
1116	8	-1.8747309714251443
1116	9	-1.8219772994456065
1116	10	-2.6314344912033363
1116	11	-1.3625541523234475
1116	12	-1.3212002306853705
1116	13	-1.906771395018012
1116	14	-1.7195492302057929
1116	15	-1.3007982493659824
1116	16	-1.321932840629188
1116	17	-1.6816389394806706
1116	18	-1.5107112518222223
1116	19	-1.898444977242285
1116	20	-2.166446939277087
1116	21	-2.5121109480428103
1116	22	-1.7421741803684867
1116	23	-1.7481464865542833
1116	24	-2.317849014776577
1116	25	-2.1886275070526224
1201	1	-0.017755240787355575
1201	2	-0.04425598304977996
1201	3	0.20908769667158467
1201	4	0.3205835954451146
1201	5	0.4019385597809091
1201	6	0.45483220387151135
1201	7	0.3194585425026517
1201	8	-0.0952125681753131
1201	9	0.26294351942414806
1201	10	0.4579994874509836
1201	11	0.10044197334188709
1201	12	-0.40950159521633367
1201	13	-0.017019208169479327
1201	14	0.437974780508938
1201	15	0.27812528803391956
1201	16	0.5261018624625535
1201	17	0.6840717829285481
1201	18	0.9178746730385683
1201	19	0.5226865785787425
1201	20	0.4349443182999906
1201	21	0.38621047682675425
1201	22	0.6478302821824116
1201	23	0.9131533957127829
1201	24	0.5713470508499583
1201	25	0.49148940076219816
1202	1	0.729743013680455
1202	2	0.7996271810330846
1202	3	0.6919937789594819
1202	4	1.0441181781094002
1202	5	0.9962093199074573
1202	6	1.0287851821471392
1202	7	0.933432512098662
1202	8	1.033436839644466
1202	9	1.4038772800151023
1202	10	1.4784069010101888
1202	11	1.2069518762266753
1202	12	1.2666905754815403
1202	13	1.2747703933383505
1202	14	1.6074531263317198
1202	15	1.2367526473994488
1202	16	1.3552029683696247
1202	17	1.5996005591608977
1202	18	1.9297662859938711
1202	19	1.7015854770862404
1202	20	1.5692234960816052
1202	21	1.5072601090647808
1202	22	1.6829018216081835
1202	23	1.7437809997720777
1202	24	1.7757191704853454
1202	25	1.6220945456537805
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2211	15	0.11360321202366919
2211	16	0.08097488691331733
2211	17	0.12413136030603766
2211	18	0.10145912732834006
2211	19	0.0017118690022144278
2211	20	-0.11065226589186494
2211	21	-0.04128298117722551
2211	22	-0.05179533767825717
2211	23	0.518712603258578
2211	24	0.20076146775225823
2211	25	0.31845913809686266
2212	1	-0.06555573743503018
2212	2	-0.13380153478038181
2212	3	-0.13058028378388897
2212	4	-0.21334612488831084
2212	5	-0.15585134193157302
2212	6	-0.34311552651850974
2212	7	-0.3537976980994202
2212	8	-0.23312432532556215
2212	9	-0.11628604304831924
2212	10	-0.04006308981909967
2212	11	-0.3086428660629963
2212	12	-0.18117551428068168
2212	13	-0.29015893886768396
2212	14	-0.20056208413264187
2212	15	-0.447810985595666
2212	16	-0.22566453896936167
2212	17	-0.2258756185055404
2212	18	-2.7636693905313265
2212	19	-1.2104162750929177
2212	20	-0.5420649740735612
2212	21	-0.5283317120171063
2212	22	-0.26905501597846637
2212	23	-0.3722701164545512
2212	24	-0.826545638364081
2212	25	-0.45052215213083713
2213	1	-0.3331406909089978
2213	2	-0.23839649177972433
2213	3	-0.5914518696166553
2213	4	-0.7075828834343341
2213	5	-0.597635466833637
2213	6	-0.5015528777426965
2213	7	-0.4657434015204842
2213	8	-0.878846193683053
2213	9	-0.742421806453855
2213	10	-0.7779441579717812
2213	11	-0.723024975782053
2213	12	-0.6668565403991096
2213	13	-0.5499522929671983
2213	14	-0.6454920821371033
2213	15	-0.8622255500066416
2213	16	-1.2865704133651192
2213	17	-0.9468995507642717
2213	18	-0.6997277356219413
2213	19	-0.6697207457086307
2213	20	-1.3145963724798762
2213	21	-0.945714010673143
2213	22	-0.808316566772227
2213	23	-1.2159372529300327
2213	24	-0.8809796369824099
2213	25	-1.0059583854175784
2214	1	-1.5407400026064586
2214	2	-3.530859775355953
2214	3	-1.8513445003119031
2214	4	-1.6670509708909762
2214	5	-1.6719716500929636
2214	6	-1.5834362099714099
2214	7	-1.800495929264919
2214	8	-1.3635620234140902
2214	9	-1.7895436512224876
2214	10	-1.9247254108100478
2214	11	-1.6370416531267367
2214	12	-2.0207246828414043
2214	13	-1.9191741968944953
2214	14	-3.135203751123477
2214	15	-3.283717084916397
2214	16	-2.531990034896914
2214	17	-2.863272510587926
2214	18	-2.1688373362640974
2214	19	-4.226605121191906
2214	20	-2.7431450949226104
2214	21	-2.748194747284508
2214	22	-2.753298144124031
2214	23	-2.9550869145473477
2214	24	-3.055725757916477
2214	25	-2.8147055226043243
2215	1	-0.8373637514420551
2215	2	-2.4767217720586814
2215	3	-1.25436728201241
2215	4	-0.782824818269205
2215	5	-0.7937871845792515
2215	6	-1.2332904250160297
2215	7	-1.5606968181473455
2215	8	-1.6230723264796332
2215	9	-1.4465319411837356
2215	10	-2.7861722856094175
2215	11	-1.7929887950269219
2215	12	-2.0644059013540925
2215	13	-1.4517895680919466
2215	14	-2.676303358527676
2215	15	-2.530344164941255
2215	16	-2.2838707032182697
2215	17	-2.504863316990104
2215	18	-1.4293834574878943
2215	19	-1.972795754856783
2215	20	-2.3062741597305987
2215	21	-2.0161959489353265
2215	22	-1.580269996180462
2215	23	-2.0945938500833847
2215	24	-1.9044805593715424
2215	25	-1.89253978927632
2216	1	-2.048187440646
2216	2	-2.0604337101930454
2216	3	-2.549442175054317
2216	4	-3.29464077874394
2216	5	-2.53445598593256
2216	6	-2.440533815095332
2216	7	-2.283192253408611
2216	8	-2.901674666131999
2216	9	-3.041407686310208
2216	10	-2.654064383241124
2216	11	-3.1713635690276014
2216	12	-3.5651760891349156
2216	13	-4.284492005304411
2216	14	-3.613956187770647
2216	15	-3.4703242277735384
2216	16	-2.5586278098899484
2216	17	-2.6974377848847766
2216	18	-2.7307995334236637
2216	19	-3.1430890393205573
2216	20	-2.9076553288407396
2216	21	-3.307366983430427
2216	22	-3.889210842536727
2216	23	-3.8688261236812913
2216	24	-3.917494763764431
2216	25	-4.125150036667257
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	110649.0
3	3.0
4	931.0
5	5484.0
6	6641.0
7	6024.0
8	5241.0
9	5048.0
10	4715.0
11	4559.0
12	4387.0
13	4259.0
14	4250.0
15	4376.0
16	4522.0
17	4845.0
18	5461.0
19	6368.0
20	8378.0
21	10722.0
22	13511.0
23	16198.0
24	18584.0
25	21694.0
26	24237.0
27	27159.0
28	30820.0
29	36464.0
30	45078.0
31	56336.0
32	71792.0
33	92140.0
34	120515.0
35	160227.0
36	222114.0
37	356902.0
38	988403.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.74435526292104	19.136203720556264	12.626610317633341	25.492830698889353
2	14.357984519428863	20.343438540027357	42.41355923553267	22.88501770501111
3	18.39740464059639	25.09942511848284	29.901374269437188	26.601795971483583
4	11.919607775123767	17.200266490919812	39.12018126485904	31.759944469097377
5	12.566162090393115	38.39855345498187	36.54655319182869	12.488731262796321
6	30.997047353560575	37.96951044747323	18.615943493514504	12.417498705451694
7	27.486431702081436	31.771761596584557	22.990945166736093	17.750861534597913
8	24.15185638615734	38.040797321372686	20.980215264492124	16.827131027977845
9	26.357470482665917	15.199523544902465	20.582844267908722	37.860161704522895
10	14.632579020654537	29.070503991743447	35.40570998499932	20.891207002602698
11	33.70423624999651	22.277335669863582	25.818606110350828	18.199821969789078
12	23.269044023733176	24.69293091324252	32.58097487159944	19.45705019142487
13	28.87380538748618	22.4125532079437	26.031052151956864	22.68258925261326
14	20.232182189664623	22.754891114765535	28.12434344944436	28.88858324612548
15	22.24483124297344	32.232711673856016	23.38793783936787	22.134519243802675
16	21.409880870185017	28.276628009140037	28.850750481159437	21.462740639515506
17	21.086096918182154	29.155622492761573	28.53616542561313	21.222115163443142
18	20.99543719711827	27.63869464334523	30.86501724767421	20.50085091186229
19	23.23107975205277	27.05286200131795	27.794000806079644	21.922057440549633
20	23.376781799720867	27.84285679546532	28.374132794682378	20.40622861013144
21	22.40433041154936	27.152857260746792	27.386432541234466	23.05637978646938
22	22.749020151864503	28.882000452850885	27.500645790878565	20.868333604406047
23	21.661141434774358	28.46506985073104	28.259846951873875	21.61394176262073
24	22.82928987714165	27.121160178268184	28.900971864560827	21.14857808002934
25	22.262649327703922	27.884909385834444	28.42926011752873	21.4231811689329
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	242.0
1	242.0
2	456.0
3	670.0
4	670.0
5	670.0
6	1314.0
7	1958.0
8	1958.0
9	1958.0
10	3055.5
11	4153.0
12	4153.0
13	4153.0
14	7616.0
15	11079.0
16	11079.0
17	11079.0
18	20792.5
19	30506.0
20	30506.0
21	30506.0
22	49870.5
23	69235.0
24	69235.0
25	69235.0
26	99993.5
27	130752.0
28	130752.0
29	130752.0
30	171380.5
31	212009.0
32	212009.0
33	212009.0
34	237319.5
35	262630.0
36	262630.0
37	262630.0
38	280261.0
39	297892.0
40	297892.0
41	297892.0
42	308216.5
43	318541.0
44	318541.0
45	318541.0
46	321105.5
47	323670.0
48	323670.0
49	323670.0
50	306458.0
51	289246.0
52	289246.0
53	289246.0
54	254380.0
55	219514.0
56	219514.0
57	219514.0
58	186815.5
59	154117.0
60	154117.0
61	154117.0
62	124765.0
63	95413.0
64	95413.0
65	95413.0
66	73073.0
67	50733.0
68	50733.0
69	50733.0
70	36937.5
71	23142.0
72	23142.0
73	23142.0
74	16044.0
75	8946.0
76	8946.0
77	8946.0
78	6059.5
79	3173.0
80	3173.0
81	3173.0
82	2107.5
83	1042.0
84	1042.0
85	1042.0
86	656.0
87	270.0
88	270.0
89	270.0
90	167.0
91	64.0
92	64.0
93	64.0
94	42.0
95	20.0
96	20.0
97	20.0
98	20.0
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.36436290098551755
2	0.03945736950072877
3	0.034594946188517745
4	0.03523264104913559
5	0.039537081358306
6	0.017536608666990562
7	0.017217761236681645
8	0.02343528612770557
9	0.020366379610982224
10	0.01929026953368962
11	0.018891710245803467
12	0.019170701747323774
13	0.019250413604901002
14	0.02638462485806307
15	0.027779582365664593
16	0.02044609146855945
17	0.021920760833738204
18	0.1156619053445605
19	0.023913557273168948
20	0.021841048976160973
21	0.025428082567136315
22	0.019330125462478233
23	0.02172148118979513
24	0.018214159456397018
25	0.02168162526100651
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2509037.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.9326932331456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.84004818924022	56.41454936014749
2	12.185933057750162	16.8001837045908
3	3.2237500959180854	6.666653292667353
4	1.1557973781048996	3.186889044183161
5	0.5215056193729266	1.797439343979777
6	0.2853455265537571	1.1801781388428325
7	0.16766857531274001	0.8090492526810174
8	0.1164634893869598	0.6422513589418407
9	0.07783054629395708	0.4828562254664533
>10	0.37923869491448847	4.636084339446804
>50	0.02481095854003968	1.1700022915995467
>100	0.017607242290816023	2.4749284684721147
>500	0.0022612235727585382	1.1020166722663574
>1k	0.0015654624734344818	1.959873483391995
>5k	1.7394027482605352E-4	0.6770450233223972
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	5856	0.23339631898612895	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5731	0.2284143278875521	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5353	0.21334878680545566	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4616	0.18397496728824644	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3694	0.14722780094514348	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3675	0.1464705382981598	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2609	0.10398411820949631	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2601	0.1036652707791874	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.971185757722983E-5	0.0
5	0.0	0.0	0.0	7.971185757722983E-5	3.985592878861492E-5
6	0.0	0.0	0.0	7.971185757722983E-5	3.985592878861492E-5
7	0.0	0.0	0.0	7.971185757722983E-5	3.985592878861492E-5
8	0.0	0.0	0.0	7.971185757722983E-5	3.985592878861492E-5
9	0.0	0.0	0.0	7.971185757722983E-5	3.985592878861492E-5
10	0.0	0.0	0.0	7.971185757722983E-5	3.985592878861492E-5
11	0.0	0.0	0.0	7.971185757722983E-5	3.985592878861492E-5
12	0.0	0.0	0.0	1.5942371515445967E-4	7.971185757722983E-5
13	0.0	0.0	0.0	1.9927964394307458E-4	7.971185757722983E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTGC	35	0.002178381	16.277103	8
TAGGACC	1070	0.0	12.602067	4
GCGGGCC	55	0.0030613479	12.095123	13
TGTACCG	135	3.7471182E-10	11.957592	5
GTACCGT	105	2.7423994E-7	11.755218	6
CGTGCGC	85	5.353326E-5	11.170784	10
GGCGAGG	1450	0.0	11.001296	19
GGTATCA	1115	0.0	10.853195	1
TGTAGGA	3390	0.0	10.700421	2
AGGACCT	2100	0.0	10.58096	5
CACCGTG	135	5.739639E-8	10.549554	7
TCCAACG	300	0.0	10.453643	18
CCAACGA	200	1.8189894E-12	10.444683	19
CTGTAGG	3420	0.0	10.392296	1
GGACCTG	2025	0.0	10.31512	6
GTCCTAC	2995	0.0	10.276245	1
AATGTCC	845	0.0	10.225359	8
GACGTGA	1720	0.0	10.2122135	7
GGACGTG	3135	0.0	10.206315	6
AAATGTC	840	0.0	10.172784	7
>>END_MODULE