FastQCFastQC Report
Thu 26 May 2016
SRR1512975_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512975_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2509037
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT57360.22861360753149515No Hit
CTGTAGGACGTGGAATATGGCAAGA53840.2145843205979027No Hit
GTCCTAAAGTGTGTATTTCTCATTT53040.2113958462948135No Hit
CTTTAGGACGTGAAATATGGCGAGG50020.19935935580065178No Hit
GTATCAACGCAGAGTACTTTTTTTT42210.16823187541674356No Hit
GTCCTACAGTGTGCATTTCTCATTT34690.13826021696770513No Hit
TATCAACGCAGAGTACTTTTTTTTT29710.11841196443097492No Hit
CTGAAGGACCTGGAATATGGCGAGA27370.10908567709443902No Hit
GGTATCAACGCAGAGTACTTTTTTT25950.1034261352064557No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG550.003034687112.1092574
GAACCGT550.00304105112.105876
TAGGACC10000.012.0833084
GGCGAGG14250.012.05414919
ATTGCGA951.0509466E-611.99017917
CCAACGA1701.8189894E-1211.7231219
GGTATCA11700.011.6199081
GGACCGT752.0510869E-411.4141066
TCCAACG2250.011.38817418
AACCGCA855.2965544E-511.1824837
TATGTCG951.3734114E-510.99099716
CCACCTT8750.010.9567413
GACGTGA16800.010.8629837
TGGCGAG31650.010.58458618
TTAGGAC20400.010.5782423
AATGTCC8050.010.5079168
ATGTCCA8050.010.5062399
AATGCCG2350.010.49973518
GGACCTG19050.010.4853996
AGGACCT19900.010.4706395
Produced by FastQC (version 0.11.5)