##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512975_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2509037
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.466500494014237	31.0	31.0	34.0	27.0	34.0
2	30.61918337593268	31.0	31.0	34.0	27.0	34.0
3	30.716156836268258	31.0	31.0	34.0	27.0	34.0
4	34.168020638994165	37.0	35.0	37.0	30.0	37.0
5	33.813432005984765	37.0	35.0	37.0	29.0	37.0
6	33.67292869734484	37.0	35.0	37.0	29.0	37.0
7	33.54201990644219	37.0	35.0	37.0	28.0	37.0
8	33.43279473359699	37.0	35.0	37.0	28.0	37.0
9	34.89249261768559	39.0	35.0	39.0	27.0	39.0
10	34.77767525947206	39.0	35.0	39.0	27.0	39.0
11	34.80252264115675	39.0	35.0	39.0	27.0	39.0
12	34.660832821516784	39.0	35.0	39.0	27.0	39.0
13	34.63957366910093	39.0	35.0	39.0	27.0	39.0
14	35.70012757882805	40.0	36.0	41.0	27.0	41.0
15	35.714293970156675	40.0	36.0	41.0	27.0	41.0
16	35.69420977052152	40.0	36.0	41.0	27.0	41.0
17	35.682489736101935	40.0	36.0	41.0	27.0	41.0
18	35.60322346780857	40.0	36.0	41.0	27.0	41.0
19	35.58549236220909	40.0	36.0	41.0	26.0	41.0
20	35.558123295909944	40.0	36.0	41.0	26.0	41.0
21	35.511528128122464	40.0	36.0	41.0	26.0	41.0
22	35.464621286971855	40.0	36.0	41.0	26.0	41.0
23	35.358791839259446	40.0	36.0	41.0	25.0	41.0
24	35.35009447847919	40.0	36.0	41.0	25.0	41.0
25	35.25344424972609	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6485968308006314
1101	2	0.7346684446865197
1101	3	0.6844576811789871
1101	4	0.46565056922149495
1101	5	0.718555139056015
1101	6	0.7332476204436986
1101	7	0.7406906499654369
1101	8	0.6765429384226707
1101	9	0.5253559669061829
1101	10	0.8810943970148841
1101	11	0.857672350987734
1101	12	0.8403140580868182
1101	13	0.9574818457347121
1101	14	1.0209325489910555
1101	15	0.9992024233412877
1101	16	1.1687886567952077
1101	17	1.0732842851371487
1101	18	0.5747800696469554
1101	19	0.9046715157606684
1101	20	0.7723056796946395
1101	21	0.9446448004364996
1101	22	0.9089125945046348
1101	23	0.8188829061964711
1101	24	0.7887993426867155
1101	25	0.9396512840344329
1102	1	0.7213391228266488
1102	2	0.6896191818965143
1102	3	0.7129624470812566
1102	4	0.5652421694220067
1102	5	0.7609280962043528
1102	6	0.7234504666910695
1102	7	0.7878761975056889
1102	8	0.75560116320991
1102	9	0.9469104479275359
1102	10	0.9805764771889969
1102	11	0.9211072597874903
1102	12	0.9809185965057594
1102	13	1.015876862717512
1102	14	1.1559496712534525
1102	15	1.1726150688797574
1102	16	1.164670097463322
1102	17	1.1542466425356324
1102	18	1.197345560727932
1102	19	1.2793490759182404
1102	20	1.2209354060522202
1102	21	1.2732005594694016
1102	22	1.296834271238808
1102	23	1.2565397870024597
1102	24	1.2190701777537996
1102	25	1.2513345924396404
1103	1	0.545192960590775
1103	2	0.4330591090613183
1103	3	0.5565936065893595
1103	4	0.48825539277454766
1103	5	0.4935850139278344
1103	6	0.5582291401121253
1103	7	0.5211068566491335
1103	8	0.5317818609061078
1103	9	0.5587562883799251
1103	10	0.732244322411546
1103	11	0.7386037762781754
1103	12	0.6759533916478944
1103	13	0.8004261949357669
1103	14	0.8779922617325866
1103	15	0.661437653827889
1103	16	0.8155885054407435
1103	17	0.9506432356879344
1103	18	1.0210540210723025
1103	19	1.0056069457195562
1103	20	1.0216753438262458
1103	21	0.6451241624145894
1103	22	0.8923017362161687
1103	23	0.9464291092862211
1103	24	0.988175988988651
1103	25	1.0077790291898125
1104	1	0.5682927109880467
1104	2	0.6899784173604466
1104	3	0.5514547648027595
1104	4	0.5468658272603903
1104	5	0.5603108682917153
1104	6	0.7019486463053468
1104	7	0.6892744923692717
1104	8	0.6221821494085233
1104	9	0.8374102045465932
1104	10	0.8173802585421299
1104	11	0.9068964927527716
1104	12	0.8543142491946654
1104	13	0.9339711769409007
1104	14	1.0593849306249226
1104	15	1.0411742277413794
1104	16	0.9813800366569936
1104	17	0.8513475993729074
1104	18	0.9161035311296928
1104	19	1.0024835804197068
1104	20	1.0191440704139012
1104	21	0.9804789898654747
1104	22	0.9278259599740295
1104	23	0.825963877652562
1104	24	0.9625885930023301
1104	25	1.0911363255886428
1105	1	0.4298935880356147
1105	2	0.5080834652466244
1105	3	0.5257981395350377
1105	4	0.39232003865802767
1105	5	0.3899796601631138
1105	6	0.40022842403744363
1105	7	0.4144400991964474
1105	8	0.6272633558793999
1105	9	0.6486979924107104
1105	10	0.692422668698903
1105	11	0.6531794566342342
1105	12	0.6466107135966581
1105	13	0.725842434624461
1105	14	0.8118948659293963
1105	15	0.774261830824301
1105	16	0.876638553500591
1105	17	0.7001179534852824
1105	18	0.8787446036121054
1105	19	0.8998737947526294
1105	20	0.996312072102576
1105	21	0.900474508479526
1105	22	1.0147336268462581
1105	23	1.0261062486870074
1105	24	0.9834220644794911
1105	25	0.9701615605989318
1106	1	0.4081061489417017
1106	2	0.4050447488727009
1106	3	0.4408098098408324
1106	4	0.37165109350308967
1106	5	0.5101820619873365
1106	6	0.5499737747381985
1106	7	0.583547670374557
1106	8	0.6764155108524577
1106	9	0.6236901942801154
1106	10	0.720146233036175
1106	11	0.5448869514444468
1106	12	0.6303603505802329
1106	13	0.6690275407328201
1106	14	0.810488916762452
1106	15	0.7910592730374688
1106	16	0.878976648175275
1106	17	0.6701040244106267
1106	18	0.8770729802026551
1106	19	0.8994697709172357
1106	20	0.9012696664061721
1106	21	0.8356713709420163
1106	22	0.8141121965077645
1106	23	0.9006616207922846
1106	24	0.8332244222039478
1106	25	0.9089473109942219
1107	1	0.48365901190897276
1107	2	0.49941378265194913
1107	3	0.3923101455047089
1107	4	0.3768380009744732
1107	5	0.3432061479172859
1107	6	0.4255882033727829
1107	7	0.39387138134340205
1107	8	0.47252639454901413
1107	9	0.4079208690463787
1107	10	0.5640818510670513
1107	11	0.40111212459788703
1107	12	0.3975599314097309
1107	13	0.42219833633807724
1107	14	0.6685040789454177
1107	15	0.5855822209850032
1107	16	0.4994164497242437
1107	17	0.4109453127049605
1107	18	0.5454121808314127
1107	19	0.6388711430456695
1107	20	0.6275716329052798
1107	21	0.613195972648171
1107	22	0.6843566580537797
1107	23	0.7018061527458528
1107	24	0.8055369007132711
1107	25	0.8090750270062017
1108	1	0.5328704154501871
1108	2	0.47390391494499085
1108	3	0.431745403602374
1108	4	0.501203237186779
1108	5	0.6166918693702428
1108	6	0.6121575731999371
1108	7	0.5883152987096949
1108	8	0.7497173503168
1108	9	0.713483415980896
1108	10	0.7977439418020396
1108	11	0.7577087506187894
1108	12	0.8596411468269309
1108	13	0.8081473262508752
1108	14	0.9626898423680856
1108	15	0.8999833002981177
1108	16	0.8059724406999464
1108	17	0.8925727084761519
1108	18	0.8429721163170498
1108	19	0.8814849718253157
1108	20	0.9095879158679878
1108	21	0.959418392837371
1108	22	1.0718925212550232
1108	23	1.0419531593082425
1108	24	1.0156039075329488
1108	25	1.0303242450991732
1109	1	-0.01770084692988405
1109	2	0.05503811123724489
1109	3	0.1272886521325738
1109	4	0.12565554954722558
1109	5	0.2134375115514402
1109	6	-0.07882113802103419
1109	7	0.07568230124028474
1109	8	0.34316801991136003
1109	9	0.485777786217902
1109	10	0.2989472602674823
1109	11	0.28525861979286304
1109	12	0.3592449858601512
1109	13	0.37601602263692513
1109	14	0.4140001278268386
1109	15	0.40468434407324594
1109	16	0.3189393989421987
1109	17	0.3863004434880395
1109	18	0.38356341056339716
1109	19	0.596981605853955
1109	20	0.5755520660781528
1109	21	0.5984154675336697
1109	22	0.5329121381750426
1109	23	0.43780726751705856
1109	24	0.05908033385211553
1109	25	0.2484614491577446
1110	1	0.3388469415299582
1110	2	0.3196824337208781
1110	3	0.3365369853861502
1110	4	0.4228448118466446
1110	5	0.3071971298180287
1110	6	0.3127247991979729
1110	7	0.3804577469863375
1110	8	0.40877253190160445
1110	9	0.5972107725590234
1110	10	0.5494750528076082
1110	11	0.45303808789485345
1110	12	0.6817659602928785
1110	13	0.274574258643689
1110	14	0.6521538866013401
1110	15	0.5839898642971093
1110	16	0.6402845206077643
1110	17	0.5377158241510074
1110	18	0.39998460941185243
1110	19	0.5346717358438084
1110	20	0.5816464894355207
1110	21	0.640887843746107
1110	22	0.5552821168416955
1110	23	0.6421668767163382
1110	24	0.5559540666336815
1110	25	0.5427888442478022
1111	1	-0.3250405526961373
1111	2	-0.1648008269784178
1111	3	-0.1826218321183184
1111	4	-0.2085476529576482
1111	5	0.05858437300481967
1111	6	0.014168777267180133
1111	7	0.09534939842328072
1111	8	0.2132867206429978
1111	9	-0.23588173040324278
1111	10	-0.09770979245176648
1111	11	0.056665605819056
1111	12	-0.08467912234926445
1111	13	0.09214801399082262
1111	14	0.2352448833277876
1111	15	0.31867546267439195
1111	16	0.0345055958593008
1111	17	0.20030042439602624
1111	18	0.11339211649115555
1111	19	0.2894083454074021
1111	20	0.18469260033464252
1111	21	0.07656052866165908
1111	22	-0.04591885518247807
1111	23	0.03155544539065147
1111	24	0.15133243307073485
1111	25	0.27169049709056736
1112	1	-0.1786530345083044
1112	2	-0.3268220279547194
1112	3	-0.19761783619474826
1112	4	-0.21518486236956846
1112	5	-0.052645154023139185
1112	6	-0.17309105005394088
1112	7	-0.10737352404009215
1112	8	0.03254137780846378
1112	9	-0.09645566088006063
1112	10	0.13924626613417246
1112	11	-0.09840249178751037
1112	12	0.11482668892140424
1112	13	-0.008518578713619718
1112	14	0.0659632757687163
1112	15	0.08394146158655502
1112	16	-0.1797237701535508
1112	17	-0.03419622668621258
1112	18	-0.04203924689439731
1112	19	0.10824721052583186
1112	20	0.283097372665182
1112	21	0.2596081910954595
1112	22	0.1759273105963146
1112	23	0.2562841742156934
1112	24	0.03773284228472562
1112	25	0.1155529744846362
1113	1	-0.0508509282570202
1113	2	-0.4702893634588392
1113	3	-0.10578674698708568
1113	4	-0.08577385001954241
1113	5	-0.003060595954551104
1113	6	-0.17896226950890082
1113	7	0.004204521663801586
1113	8	0.21541309595801295
1113	9	0.09935958744051021
1113	10	-0.18762785748342026
1113	11	0.06745452953902031
1113	12	-0.04693340964411874
1113	13	0.07841914313213749
1113	14	0.1024836195746417
1113	15	0.17314137944862296
1113	16	-0.12252526037033817
1113	17	0.018145467115303404
1113	18	0.19949286023692991
1113	19	0.12309117778799816
1113	20	0.2989339181783919
1113	21	0.0433198869504281
1113	22	-0.14407491886186818
1113	23	0.03271833186185091
1113	24	-0.2657420029732762
1113	25	-0.2841934539820201
1114	1	-0.6835322429579378
1114	2	-0.5144884086562129
1114	3	-0.5097644634295406
1114	4	-0.34826917380485156
1114	5	-0.5079011699952218
1114	6	-0.8411639371535529
1114	7	-0.7561297606766928
1114	8	-1.061978539739144
1114	9	-0.9063539862596741
1114	10	-0.851766890655206
1114	11	-0.8033022855639587
1114	12	-0.7357152756385119
1114	13	-0.7889742746700819
1114	14	-1.8982103292369317
1114	15	-1.1762420266902183
1114	16	-1.147625702998866
1114	17	-1.2454726118333994
1114	18	-1.3161185829059505
1114	19	-1.25276503676006
1114	20	-0.599304637592077
1114	21	-0.6230921270456165
1114	22	-0.7507668247725192
1114	23	-0.791744783634492
1114	24	-1.4676624457466971
1114	25	-1.3932187584249718
1115	1	-1.365138579202565
1115	2	-1.116747440469549
1115	3	-0.8464363824393537
1115	4	-2.10705962126584
1115	5	-1.338376271507066
1115	6	-1.2861036456765902
1115	7	-1.043872261202452
1115	8	-1.062916591897995
1115	9	-1.4488153385107339
1115	10	-1.2363876547037407
1115	11	-1.2239686306341397
1115	12	-1.5166753782018745
1115	13	-1.0949392528578414
1115	14	-1.9051415797874043
1115	15	-1.8691586319434919
1115	16	-1.270621922480153
1115	17	-1.3525627807771556
1115	18	-1.259032828986129
1115	19	-1.112539888514121
1115	20	-1.172742887472097
1115	21	-1.1433366407324144
1115	22	-1.4524581496661568
1115	23	-1.716194062761069
1115	24	-1.2458874803029616
1115	25	-1.1255084460075437
1116	1	-0.8839958184545402
1116	2	-0.9978885946370184
1116	3	-0.7710777133306834
1116	4	-0.8651949435459017
1116	5	-0.9393619086769149
1116	6	-1.5506472161412148
1116	7	-0.9232100286981151
1116	8	-1.1462900897451718
1116	9	-1.2964974444165236
1116	10	-1.2561747024513181
1116	11	-1.1491160343999667
1116	12	-1.779789091973484
1116	13	-1.4372533714877491
1116	14	-1.7465158487932513
1116	15	-1.683014184285888
1116	16	-1.8110575467973575
1116	17	-1.7636666622757744
1116	18	-2.3683505945538883
1116	19	-1.85996333495072
1116	20	-1.5425335500259152
1116	21	-1.7097245656729712
1116	22	-2.93785433243076
1116	23	-2.7929524651773647
1116	24	-1.9449548602913964
1116	25	-2.4057322426787024
1201	1	0.5558170157803346
1201	2	0.5509696752334072
1201	3	0.47239127360619904
1201	4	0.41115214015851365
1201	5	0.5708664040368205
1201	6	0.531679160455802
1201	7	0.5591484813403156
1201	8	0.6092498859599971
1201	9	0.7938985515619308
1201	10	0.6600206929597761
1201	11	0.787323685352348
1201	12	0.5933752307328817
1201	13	0.6800862844973707
1201	14	0.6221916885871792
1201	15	0.7358411887061109
1201	16	0.7046043668618296
1201	17	0.6939557603064017
1201	18	0.6484308646041583
1201	19	0.49335025963466705
1201	20	0.6827511505994437
1201	21	0.5576703180630318
1201	22	0.6173273579650669
1201	23	0.6242765687450671
1201	24	0.6195878089597784
1201	25	0.4360377846359995
1202	1	0.7515725089939842
1202	2	0.827872057000949
1202	3	0.7976723107924784
1202	4	0.8196022581420621
1202	5	0.9189787736618982
1202	6	1.05866938050913
1202	7	0.9669868648856692
1202	8	0.8310661333901805
1202	9	1.0387295729306132
1202	10	1.0226182403619006
1202	11	1.029909972426232
1202	12	1.0313918300207305
1202	13	1.059679406994114
1202	14	1.0528554921895221
1202	15	1.30373799604358
1202	16	1.1511393682015694
1202	17	1.2885282095689021
1202	18	1.242293118563346
1202	19	1.3971673169823262
1202	20	1.5061832283294478
1202	21	1.4657596625083897
1202	22	1.3270572385830448
1202	23	1.2878953106170243
1202	24	1.3066937634929374
1202	25	1.3621175609556175
1203	1	0.6475512086132014
1203	2	0.4543402764048423
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2211	13	-0.03250607866760902
2211	14	-1.1343307599621753
2211	15	-0.5064410609246934
2211	16	-0.22297922972164486
2211	17	-0.06685695251829316
2211	18	-0.09922450351465528
2211	19	-0.0712832893165043
2211	20	-0.1123429570376473
2211	21	-0.2637809311161732
2211	22	0.012765270099990289
2211	23	-0.26919479176990535
2211	24	-0.2845524650080762
2211	25	-0.37763555560663065
2212	1	-0.1804335840835769
2212	2	-0.07086881652609733
2212	3	-0.05776506677028337
2212	4	0.03342989474517566
2212	5	-0.17490784897253775
2212	6	-0.1510578595557064
2212	7	-0.11599848400261692
2212	8	-0.31929594567785813
2212	9	-0.40691683532372025
2212	10	-0.32237237583769485
2212	11	-0.22631240813036158
2212	12	-0.1424150553696677
2212	13	-0.1321548898809226
2212	14	-0.22798004142561012
2212	15	-0.2121791428475106
2212	16	-0.13953437591603546
2212	17	-0.26302132273441003
2212	18	-0.10879193897469719
2212	19	-0.06284033243619547
2212	20	-0.3540131131100992
2212	21	-0.23297673783400086
2212	22	-0.20888497059875277
2212	23	-0.6207884432058961
2212	24	-0.41360502342166683
2212	25	-0.40581552838458634
2213	1	-0.41641653535641865
2213	2	-0.45902898296437655
2213	3	-0.36875412893585846
2213	4	-0.37390948650190126
2213	5	-0.3685209586411631
2213	6	-0.2301605005954741
2213	7	-0.7830957484219709
2213	8	-0.41776643688263704
2213	9	-0.5829038500687673
2213	10	-0.5146321610479561
2213	11	-0.5718637264785542
2213	12	-0.7509036705291692
2213	13	-0.5947901288234618
2213	14	-0.5158882677610066
2213	15	-0.6286448075931972
2213	16	-0.9169804884592523
2213	17	-0.8516141862251487
2213	18	-1.0991836007490292
2213	19	-1.1875725209031245
2213	20	-0.7768006260889635
2213	21	-1.384651086248752
2213	22	-0.8301396207287297
2213	23	-0.9819989091080856
2213	24	-1.1026414182820972
2213	25	-1.069342535393588
2214	1	-1.101636072936902
2214	2	-1.0459767637778192
2214	3	-0.8544720967250683
2214	4	-1.483767014749553
2214	5	-1.2791344168704768
2214	6	-2.7280522672304777
2214	7	-1.372255719849072
2214	8	-1.8414253638278169
2214	9	-1.3737996743503302
2214	10	-1.6274747474355706
2214	11	-1.4230444451704827
2214	12	-1.4554911676755609
2214	13	-1.6228841150132283
2214	14	-1.690291955727254
2214	15	-2.0381905868307086
2214	16	-1.507291721978902
2214	17	-1.5881830815290314
2214	18	-1.605038468083869
2214	19	-3.2842034223487033
2214	20	-1.9837391280736014
2214	21	-2.2629794978752713
2214	22	-2.1232601546786896
2214	23	-2.0464258923350016
2214	24	-1.38601905925033
2214	25	-1.1907653382381156
2215	1	-1.46668390275795
2215	2	-1.3284854763519078
2215	3	-1.30667049993464
2215	4	-1.1288741033991556
2215	5	-1.7031394512059563
2215	6	-1.6562329170183077
2215	7	-1.7967872032215908
2215	8	-2.4662183758406897
2215	9	-1.9463633939441465
2215	10	-1.4481619172692888
2215	11	-2.1212210127373012
2215	12	-2.730006479638405
2215	13	-2.1317564650521703
2215	14	-2.1916103798688056
2215	15	-3.1497704063650502
2215	16	-2.7167900933301894
2215	17	-2.3324582208030264
2215	18	-2.9598021292910204
2215	19	-3.643682230533983
2215	20	-3.056540634013416
2215	21	-2.2933112479852014
2215	22	-3.119934900993485
2215	23	-3.117351999289191
2215	24	-2.156011464138821
2215	25	-2.398127751036668
2216	1	-1.2498677632738548
2216	2	-1.089324716994195
2216	3	-1.4731141880645282
2216	4	-1.0795777220734166
2216	5	-0.9935454444393983
2216	6	-1.3915060405526702
2216	7	-2.7301835254742457
2216	8	-1.945666870371955
2216	9	-1.9467111196302014
2216	10	-1.8344679248234854
2216	11	-2.333492440157954
2216	12	-1.8465504130111228
2216	13	-2.2464882642752713
2216	14	-2.3582604881694706
2216	15	-2.3208712468140007
2216	16	-2.0755387283838047
2216	17	-2.452449650729811
2216	18	-2.1651923249482508
2216	19	-2.327053603356781
2216	20	-2.8481275296330537
2216	21	-2.6383223689418287
2216	22	-2.9243131343500863
2216	23	-2.140398671505153
2216	24	-2.312044191525466
2216	25	-2.1806985044870757
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	66820.0
3	2.0
4	1036.0
5	9873.0
6	11323.0
7	8595.0
8	6915.0
9	6139.0
10	5304.0
11	4900.0
12	4400.0
13	3963.0
14	3963.0
15	3987.0
16	4080.0
17	4353.0
18	4865.0
19	5584.0
20	7186.0
21	9112.0
22	11931.0
23	14211.0
24	16875.0
25	19535.0
26	21916.0
27	24049.0
28	27296.0
29	32994.0
30	41708.0
31	53397.0
32	67675.0
33	87529.0
34	115138.0
35	151527.0
36	208387.0
37	328220.0
38	1114249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.23037067498322	19.647533094627363	13.580394148047429	27.541702082341995
2	13.13767198386308	20.66958970980407	41.290475319336686	24.902262986996167
3	16.684170062059668	25.090263714724014	30.447976653991155	27.777589569225164
4	11.076037049159543	17.622031755625127	38.45666242615145	32.84526876906388
5	12.217847339825992	38.54849272627054	36.62063733775997	12.613022596143491
6	31.143378981591653	38.25399300936672	18.217568004709882	12.385060004331752
7	27.169826817337484	31.855052367052995	23.013076114332375	17.96204470127715
8	24.23029786263311	38.254173295297065	20.715664758073103	16.79986408399673
9	25.974264698552624	14.96433095672624	20.48281502139026	38.578589323330874
10	14.240372745647697	29.35535558539947	35.398268574442596	21.006003094510238
11	33.63084301007039	22.268306351133425	25.745594351076033	18.35525628772014
12	23.083950697988108	24.726221728312783	32.58653003728503	19.603297536414086
13	28.62492352271799	22.39298660974963	26.067164491423583	22.914925376108798
14	20.08570361504082	22.725209147192828	27.988436269744376	29.200650968021975
15	22.06867551516601	32.269281358364225	23.38319582287058	22.278847303599186
16	21.349330871437704	28.342134929100915	28.715684539327345	21.592849660134032
17	21.03553509955688	29.156123610399604	28.51271645075577	21.295624839287743
18	20.901323595585332	27.74473006964132	30.683830483790548	20.670115850982803
19	23.129651086088764	27.054867819576025	27.76568111769154	22.04979997664368
20	23.208211667340265	27.829048219192497	28.401725365276988	20.561014748190257
21	22.222102654054616	27.045641959499072	27.48485569517132	23.247399691274993
22	22.6640861085891	28.693217824662874	27.583540652159535	21.059155414588496
23	21.49204773946956	28.388205027032303	28.29785156444609	21.821895669052047
24	22.7760564424396	27.12123470626544	28.875774501262242	21.22693435003272
25	22.161041420797567	27.810241466872487	28.43253192462047	21.596185187709473
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	167.0
1	167.0
2	316.0
3	465.0
4	465.0
5	465.0
6	1175.5
7	1886.0
8	1886.0
9	1886.0
10	2893.5
11	3901.0
12	3901.0
13	3901.0
14	7420.0
15	10939.0
16	10939.0
17	10939.0
18	20640.5
19	30342.0
20	30342.0
21	30342.0
22	49783.0
23	69224.0
24	69224.0
25	69224.0
26	100097.0
27	130970.0
28	130970.0
29	130970.0
30	170802.0
31	210634.0
32	210634.0
33	210634.0
34	236135.5
35	261637.0
36	261637.0
37	261637.0
38	280131.0
39	298625.0
40	298625.0
41	298625.0
42	310025.5
43	321426.0
44	321426.0
45	321426.0
46	323298.0
47	325170.0
48	325170.0
49	325170.0
50	308368.5
51	291567.0
52	291567.0
53	291567.0
54	254981.5
55	218396.0
56	218396.0
57	218396.0
58	186063.5
59	153731.0
60	153731.0
61	153731.0
62	124017.0
63	94303.0
64	94303.0
65	94303.0
66	72298.5
67	50294.0
68	50294.0
69	50294.0
70	36329.0
71	22364.0
72	22364.0
73	22364.0
74	15527.0
75	8690.0
76	8690.0
77	8690.0
78	5845.0
79	3000.0
80	3000.0
81	3000.0
82	1990.0
83	980.0
84	980.0
85	980.0
86	621.0
87	262.0
88	262.0
89	262.0
90	161.0
91	60.0
92	60.0
93	60.0
94	32.0
95	4.0
96	4.0
97	4.0
98	2.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07604511212867726
2	3.1884743030891933E-4
3	0.0
4	0.027660014579298748
5	0.07154139217556377
6	0.07843646785599415
7	0.03491379361882666
8	0.06400862163451555
9	0.0446386402432487
10	0.10613633836408151
11	0.003945736950072876
12	0.010163261841096803
13	0.00486242331221102
14	0.006297236748601157
15	0.005460262244040243
16	0.01598222744423458
17	0.02678318414594922
18	0.0015145252939673668
19	0.003188474303089193
20	0.0047428555258451745
21	3.1884743030891933E-4
22	7.572626469836834E-4
23	7.971185757722983E-5
24	1.1956778636584476E-4
25	0.003188474303089193
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2509037.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.30638201373962
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.33077112394434	55.554107218641654
2	12.505474738435888	17.084074694935445
3	3.3341130600332254	6.8322360106688365
4	1.1885875102052421	3.2475245011535563
5	0.535270025033602	1.8281179405224592
6	0.28318655353355815	1.1606069344090526
7	0.18197951533946144	0.8701253605416709
8	0.11671605185773068	0.6377960980263676
9	0.07891066789772358	0.48510920037431216
>10	0.39764865973558794	4.759658927964082
>50	0.02591426657710204	1.2213498145416113
>100	0.017744932360216098	2.5948899454677252
>500	0.00169530089435015	0.8313632083357005
>1k	0.0017537595458651614	2.0374787562476735
>5k	2.3383460611535483E-4	0.8555613881697729
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	5736	0.22861360753149515	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5384	0.2145843205979027	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5304	0.2113958462948135	No Hit
CTTTAGGACGTGAAATATGGCGAGG	5002	0.19935935580065178	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4221	0.16823187541674356	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3469	0.13826021696770513	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2971	0.11841196443097492	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2737	0.10908567709443902	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2595	0.1034261352064557	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.985592878861492E-5	0.0
3	0.0	0.0	0.0	3.985592878861492E-5	0.0
4	0.0	0.0	0.0	3.985592878861492E-5	0.0
5	0.0	0.0	0.0	3.985592878861492E-5	0.0
6	0.0	0.0	0.0	7.971185757722983E-5	0.0
7	0.0	0.0	0.0	7.971185757722983E-5	0.0
8	0.0	0.0	0.0	7.971185757722983E-5	0.0
9	0.0	0.0	0.0	7.971185757722983E-5	0.0
10	0.0	0.0	0.0	1.1956778636584474E-4	0.0
11	0.0	0.0	0.0	1.1956778636584474E-4	0.0
12	0.0	0.0	0.0	1.5942371515445967E-4	3.985592878861492E-5
13	0.0	0.0	0.0	1.5942371515445967E-4	3.985592878861492E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	55	0.0030346871	12.109257	4
GAACCGT	55	0.003041051	12.10587	6
TAGGACC	1000	0.0	12.083308	4
GGCGAGG	1425	0.0	12.054149	19
ATTGCGA	95	1.0509466E-6	11.990179	17
CCAACGA	170	1.8189894E-12	11.72312	19
GGTATCA	1170	0.0	11.619908	1
GGACCGT	75	2.0510869E-4	11.414106	6
TCCAACG	225	0.0	11.388174	18
AACCGCA	85	5.2965544E-5	11.182483	7
TATGTCG	95	1.3734114E-5	10.990997	16
CCACCTT	875	0.0	10.95674	13
GACGTGA	1680	0.0	10.862983	7
TGGCGAG	3165	0.0	10.584586	18
TTAGGAC	2040	0.0	10.578242	3
AATGTCC	805	0.0	10.507916	8
ATGTCCA	805	0.0	10.506239	9
AATGCCG	235	0.0	10.499735	18
GGACCTG	1905	0.0	10.485399	6
AGGACCT	1990	0.0	10.470639	5
>>END_MODULE