FastQCFastQC Report
Thu 26 May 2016
SRR1512974_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512974_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466781
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21400.14589771751883887No Hit
GTCCTACAGTGGACATTTCTAAATT20490.13969365569911255No Hit
GTCCTAAAGTGTGTATTTCTCATTT19570.13342141737587274No Hit
CTGTAGGACGTGGAATATGGCAAGA18400.12544476646479605No Hit
CTTTAGGACGTGAAATATGGCGAGG16640.11344570184642425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4250.012.7401014
TTCGTGA550.003077309812.08617810
TAGGCTA1406.8030204E-1011.5347415
ATAGGAC1657.2759576E-1211.5145733
GTTCTAG1701.0913936E-1111.2007461
TGTAGGA12150.010.9459522
GTGTTAG1408.067218E-910.8807231
GGACGTG9850.010.6042626
GTAGGAC10950.010.5839423
AGGACGT10400.010.5039215
AATCCCG1002.410312E-510.44519819
ATACTCT1553.6870915E-910.4145686
CTGTAGG11800.010.2467831
GACGTGG5100.010.2403917
ATAAGAC1303.8418875E-710.2302553
GTCTAAT1409.379437E-810.2006781
TCGGGCC750.002643279310.13524413
ACTACTC856.6203764E-410.0542028
GTCCTAT951.608078E-410.021721
GATATAC2002.3646862E-119.9966651
Produced by FastQC (version 0.11.5)