##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512974_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1466781
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.454064376345208	31.0	30.0	31.0	18.0	34.0
2	28.600834071343982	31.0	30.0	34.0	16.0	34.0
3	28.898222024964873	31.0	30.0	34.0	22.0	34.0
4	32.31148003689712	35.0	33.0	37.0	22.0	37.0
5	32.51638724526702	35.0	33.0	37.0	25.0	37.0
6	32.52032580187499	36.0	33.0	37.0	24.0	37.0
7	32.28360743696571	35.0	33.0	37.0	22.0	37.0
8	32.35801868172549	36.0	33.0	37.0	22.0	37.0
9	33.63525706973297	38.0	34.0	39.0	20.0	39.0
10	33.486271638370006	38.0	33.0	39.0	19.0	39.0
11	33.575085169497015	38.0	34.0	39.0	19.0	39.0
12	33.45815769361616	38.0	34.0	39.0	18.0	39.0
13	33.467900115968234	38.0	34.0	39.0	18.0	39.0
14	34.436153045342145	39.0	34.0	41.0	17.0	41.0
15	34.35112740075035	39.0	34.0	41.0	17.0	41.0
16	34.26977374263779	39.0	34.0	41.0	17.0	41.0
17	34.197998201503836	39.0	33.0	41.0	17.0	41.0
18	33.89672623247779	39.0	33.0	41.0	11.0	41.0
19	34.11516852209021	39.0	34.0	41.0	17.0	41.0
20	34.182257610372645	39.0	34.0	41.0	17.0	41.0
21	34.12836067552007	39.0	34.0	41.0	12.0	41.0
22	34.03849449917882	39.0	34.0	41.0	10.0	41.0
23	33.77335266818973	39.0	34.0	41.0	2.0	41.0
24	33.75851132513988	39.0	34.0	41.0	2.0	41.0
25	33.67001345122414	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7117235292092623
1101	2	0.9173698451668848
1101	3	1.0731871759189637
1101	4	1.0885185443340788
1101	5	0.8543122134215437
1101	6	1.0682592382893503
1101	7	1.2368947327188025
1101	8	0.9674765877858604
1101	9	0.9009944438590196
1101	10	1.0056955458446737
1101	11	1.1676780690125952
1101	12	1.420854378759465
1101	13	1.2546020728075575
1101	14	1.1419457535270965
1101	15	1.0170737466654671
1101	16	1.5048142696579063
1101	17	1.6540304962451984
1101	18	1.835540484479182
1101	19	1.2830330492793678
1101	20	1.4781442539799627
1101	21	1.4856088976318134
1101	22	1.4176440250207207
1101	23	1.5853620139457973
1101	24	1.3079915589007953
1101	25	1.4540896276661783
1102	1	0.7354351504287209
1102	2	0.7714032608928321
1102	3	0.8138631814447521
1102	4	1.037564079573329
1102	5	0.9917410851214967
1102	6	0.974721614517037
1102	7	1.0808128528390455
1102	8	1.093856200749066
1102	9	1.2243603558649454
1102	10	1.3092517098855083
1102	11	1.2917098399750344
1102	12	1.3093691418633284
1102	13	1.3018365149277145
1102	14	1.4385784670239659
1102	15	1.4796441799950202
1102	16	1.4551184736888843
1102	17	1.3285368162296933
1102	18	1.6005057089276207
1102	19	1.5762580907652506
1102	20	1.5817759381483043
1102	21	1.4120122940298572
1102	22	1.7516773480839376
1102	23	1.8766160418789823
1102	24	1.7633925993488475
1102	25	1.6696868505640623
1103	1	0.5245435901870046
1103	2	0.8364873693551758
1103	3	0.7958797366042738
1103	4	0.9811185750204316
1103	5	0.5106250198103837
1103	6	0.880523298945036
1103	7	0.7371833667657697
1103	8	0.7761775370880528
1103	9	1.1147302343289311
1103	10	1.1166229270375823
1103	11	1.1552668049929622
1103	12	1.205372352305396
1103	13	1.2698499259019442
1103	14	1.4696122384049488
1103	15	1.3957589578164686
1103	16	1.453500453049898
1103	17	1.3521682749479567
1103	18	1.6296714909157188
1103	19	1.724386788533451
1103	20	1.5814213138016484
1103	21	1.352863061177942
1103	22	1.4515254845367593
1103	23	1.786798955761732
1103	24	1.5821893461410426
1103	25	1.5417747522269565
1104	1	0.4305891189110902
1104	2	0.36383975138039304
1104	3	0.4271209908569098
1104	4	0.7999948328902349
1104	5	0.5155559206342488
1104	6	0.49135847987274417
1104	7	0.7502845237463092
1104	8	0.7563127583202558
1104	9	0.8122069239177137
1104	10	0.8600061496859297
1104	11	0.8760224521947251
1104	12	0.7699107560570084
1104	13	0.8490151692321319
1104	14	1.0222030277660252
1104	15	1.133136919666903
1104	16	0.8631313267358891
1104	17	0.9841232699629217
1104	18	1.1644145552134617
1104	19	1.1757483637883865
1104	20	1.179386850893863
1104	21	1.1357355504672597
1104	22	1.1006490262416762
1104	23	1.2796710573671106
1104	24	1.0648353694913197
1104	25	0.8708641045972811
1105	1	0.2145969984927305
1105	2	0.4195748735408671
1105	3	0.5358390264181523
1105	4	0.717211642785017
1105	5	0.32194693582693645
1105	6	0.19168108462158528
1105	7	0.5111249642082711
1105	8	0.5062380821326684
1105	9	0.3808752742587629
1105	10	0.5800705874108729
1105	11	0.6665401413597749
1105	12	0.6924170463158816
1105	13	0.6090756483502062
1105	14	0.8399554979602328
1105	15	0.8913221263819864
1105	16	0.911472827862049
1105	17	0.7886086175993512
1105	18	0.7502695554966081
1105	19	0.7726442225754013
1105	20	0.6533862397341679
1105	21	0.5963778977546781
1105	22	0.4044429746028442
1105	23	0.5947906378705738
1105	24	0.7324462760683801
1105	25	0.6895512257547125
1106	1	0.4314022446743593
1106	2	0.589284179344876
1106	3	0.7719035609551952
1106	4	0.6678405651963075
1106	5	0.6149311808063516
1106	6	0.7028806480338332
1106	7	0.4186495745516936
1106	8	0.6895565673360977
1106	9	0.7542854650767836
1106	10	0.9169451042556389
1106	11	0.9246248253455605
1106	12	0.9450076494591073
1106	13	0.9400643774103017
1106	14	1.1416825909691681
1106	15	1.1266999917391871
1106	16	1.076998288904214
1106	17	1.070500136598497
1106	18	1.3073108332977554
1106	19	0.7367586657414336
1106	20	0.8672440457497643
1106	21	0.8876136458685693
1106	22	1.0236887332477167
1106	23	1.1945141516891553
1106	24	1.028378324915721
1106	25	1.1247249088674351
1107	1	0.75841710548778
1107	2	0.8109407116136325
1107	3	0.8657389418298074
1107	4	1.0940746291523666
1107	5	0.9122432525059736
1107	6	0.851541348463563
1107	7	1.0087896213876633
1107	8	0.9633593356207513
1107	9	1.2710000004998747
1107	10	1.0569739995342005
1107	11	1.032056739073795
1107	12	1.1706789497879058
1107	13	1.0902270725758783
1107	14	1.3429489885374153
1107	15	1.5859553346118247
1107	16	1.2609140638138285
1107	17	1.4838491955464121
1107	18	1.8274915104189446
1107	19	1.7554381554579166
1107	20	1.670788803560292
1107	21	1.6626813226440404
1107	22	1.541520973783598
1107	23	1.7280073605093023
1107	24	1.5639844090349655
1107	25	1.670245737024132
1108	1	0.7431075801702853
1108	2	0.873700972836378
1108	3	0.6978716252081512
1108	4	0.8358638287516911
1108	5	0.7518172529383449
1108	6	0.9213064822257593
1108	7	0.9852784066919966
1108	8	0.9155307134231876
1108	9	0.9868234936340556
1108	10	0.9937404958807718
1108	11	0.7950992120069813
1108	12	0.8469629051534469
1108	13	0.884290719525346
1108	14	1.0828694555029728
1108	15	1.0179812686508498
1108	16	1.0181820945273543
1108	17	1.1012823144493211
1108	18	1.285766565642355
1108	19	1.348107692126156
1108	20	1.4159163525678622
1108	21	1.1089846712190194
1108	22	0.9737691106237278
1108	23	1.186523017491865
1108	24	1.3260423607992635
1108	25	1.3359002251138108
1109	1	0.16451502608256163
1109	2	0.08764458179154744
1109	3	0.28968865006968514
1109	4	0.41293386047837544
1109	5	0.4317939508537023
1109	6	0.3322279900498799
1109	7	0.4958847986330568
1109	8	0.5530918813953676
1109	9	0.8187394627319051
1109	10	1.0064204148612177
1109	11	0.42897757512515966
1109	12	0.7531981845095927
1109	13	0.6396690199071386
1109	14	0.41727767217651035
1109	15	0.7227040634670345
1109	16	0.682882662739722
1109	17	0.5094645726005709
1109	18	-1.2899981908508593
1109	19	0.36844252515776077
1109	20	0.5462244778255254
1109	21	0.6309639144390076
1109	22	0.7263762554013695
1109	23	0.2610165556445452
1109	24	0.7833092055177602
1109	25	0.7596181623415816
1110	1	0.18323843033788023
1110	2	0.11019777328090896
1110	3	0.14585886283564165
1110	4	0.10782747749965438
1110	5	0.35434714234306597
1110	6	0.3135045857945613
1110	7	0.3712039475692279
1110	8	0.20883656224643232
1110	9	0.560441590391477
1110	10	0.6821650957122785
1110	11	0.4796158729974991
1110	12	-0.09020607080955756
1110	13	0.1966902965028865
1110	14	0.3466393743041678
1110	15	0.4031295953819267
1110	16	0.6203294712503649
1110	17	0.15074116834525597
1110	18	0.7606401070214801
1110	19	0.5139744400513777
1110	20	0.5326074565489307
1110	21	0.4756447655028424
1110	22	0.5285039149758433
1110	23	0.4473995343679533
1110	24	0.5186283544539307
1110	25	0.6383415665968997
1111	1	-0.09175344318457945
1111	2	0.2926774570870556
1111	3	0.31118724640538176
1111	4	0.13534336106938838
1111	5	0.1421197487260457
1111	6	0.26163106333593333
1111	7	0.2579337791295089
1111	8	0.03280523836936311
1111	9	0.4124994361361587
1111	10	0.179682456823393
1111	11	0.012999073352112589
1111	12	0.23208485708642712
1111	13	0.131971029363406
1111	14	0.18768990621906312
1111	15	0.3715131651218755
1111	16	0.19463687373263383
1111	17	0.267116759361798
1111	18	0.5212392500474863
1111	19	0.22244001333742602
1111	20	0.1960756155323864
1111	21	0.3644025225950358
1111	22	0.10173271225716718
1111	23	0.5325550455972632
1111	24	0.5363721487801669
1111	25	-0.009050572883005259
1112	1	-0.47259883486286824
1112	2	-0.16807517250382986
1112	3	-0.021729492901918945
1112	4	0.047268410465022725
1112	5	-0.1258522701024063
1112	6	-0.11639659821683068
1112	7	-0.5668624638969888
1112	8	-0.22666419445257446
1112	9	0.037031993667540064
1112	10	-0.13758385826261588
1112	11	-0.034779212910095225
1112	12	0.00968175968986884
1112	13	-0.3395974271684423
1112	14	-0.18222379670980615
1112	15	-0.08246463590089803
1112	16	0.1869278866336046
1112	17	0.005542201016844217
1112	18	-0.07726072089626257
1112	19	-0.08167943691827162
1112	20	-0.09014336691763702
1112	21	-0.08045778946911497
1112	22	-0.18498313211754436
1112	23	0.12569740670837604
1112	24	-0.20284943420448798
1112	25	-0.01507248129769323
1113	1	-0.17574279944751225
1113	2	0.13046591920389616
1113	3	-0.1440503729945526
1113	4	0.03384721525109313
1113	5	0.028045068128385253
1113	6	0.042088572599205065
1113	7	0.04797016302585888
1113	8	0.12760788261170575
1113	9	0.25344620692438014
1113	10	-0.025335987007515826
1113	11	0.05453547529378966
1113	12	0.3037522606040639
1113	13	0.08989984655218564
1113	14	0.25646291401464794
1113	15	-0.17273247993851015
1113	16	-0.1944277691502876
1113	17	-0.01323623736598023
1113	18	-0.01925484612209516
1113	19	-0.12603072014508143
1113	20	-0.22915788351723165
1113	21	-0.08057557488583456
1113	22	0.13214830372292852
1113	23	-0.31566248935052243
1113	24	0.2572988666955709
1113	25	0.23554861026506302
1114	1	-1.4396098407090463
1114	2	-1.2599613418478874
1114	3	-1.0941429745215032
1114	4	-1.4012071750561113
1114	5	-1.4106247460926085
1114	6	-1.8369944404061513
1114	7	-1.8671674190570187
1114	8	-1.3342248422293679
1114	9	-1.5722196376980406
1114	10	-1.6895447488473074
1114	11	-2.4539361058361457
1114	12	-1.791856384997626
1114	13	-1.64891915486227
1114	14	-2.1256466654562374
1114	15	-2.0377233058050095
1114	16	-2.840613827908129
1114	17	-4.775458442135033
1114	18	-3.9013868653814185
1114	19	-2.7244017037034496
1114	20	-1.9651825702764825
1114	21	-2.9028214087521107
1114	22	-3.093366147126833
1114	23	-2.8714528523589813
1114	24	-2.541914768318275
1114	25	-2.488208244155313
1115	1	-0.9298623001121342
1115	2	-0.6180139178999511
1115	3	-0.7645987133441245
1115	4	-0.7088228021412917
1115	5	-0.7064171432290536
1115	6	-0.698956453554203
1115	7	-2.0329180196699888
1115	8	-1.3269968871893063
1115	9	-0.9923510374372739
1115	10	-0.7990509940912744
1115	11	-1.6321509402899217
1115	12	-1.2834142789181513
1115	13	-1.1326097054874396
1115	14	-1.208781679374951
1115	15	-2.750274460904521
1115	16	-1.2716733948205317
1115	17	-1.2293639209209104
1115	18	-1.2189717792114294
1115	19	-1.3548526602412068
1115	20	-1.1870442362241889
1115	21	-1.9267635301801391
1115	22	-2.150102059985848
1115	23	-1.3037693846854452
1115	24	-1.3205862625076392
1115	25	-1.6681150542259005
1116	1	-1.4753800048539034
1116	2	-2.515600636347287
1116	3	-2.0754401293256137
1116	4	-1.8071344599034944
1116	5	-2.321725114378687
1116	6	-2.0844946676582197
1116	7	-1.68657088926496
1116	8	-2.559582454347275
1116	9	-2.6404083423061593
1116	10	-3.6785898218847457
1116	11	-2.1482868948828724
1116	12	-2.1189044770443566
1116	13	-2.867048773932712
1116	14	-2.648044078363604
1116	15	-2.3159369943677532
1116	16	-2.3243253637740153
1116	17	-2.6209497611060364
1116	18	-2.4047185184267335
1116	19	-2.8924132894270613
1116	20	-3.241081730958843
1116	21	-3.6995823092763054
1116	22	-2.8471371541702695
1116	23	-2.607370110569665
1116	24	-3.128292590720097
1116	25	-2.837092505365728
1201	1	0.3287949489164568
1201	2	0.3527278522177326
1201	3	0.5927480810162749
1201	4	0.6924565117118178
1201	5	0.7278724589757175
1201	6	0.7607458976183139
1201	7	0.6454875050384032
1201	8	0.11945566178884093
1201	9	0.6065714493862657
1201	10	0.7023403037718481
1201	11	0.36078193855141194
1201	12	-0.21303829045336187
1201	13	0.12504939846838425
1201	14	0.836158204510788
1201	15	0.38701269060005217
1201	16	0.9913390610957507
1201	17	0.9420213987252097
1201	18	1.2634221873375964
1201	19	0.824629885946564
1201	20	0.7811958201242959
1201	21	0.6580111926563603
1201	22	0.9673637578477638
1201	23	1.3308222424508216
1201	24	0.9440799345883164
1201	25	0.8234181269271872
1202	1	0.4817908944222573
1202	2	0.6662952455485964
1202	3	0.3435657186276053
1202	4	0.8892095469668249
1202	5	0.8261074624012608
1202	6	0.7687891829940554
1202	7	0.766821332548318
1202	8	0.8344612212753475
1202	9	1.1488976733156449
1202	10	1.288062531609718
1202	11	0.8727146182239025
1202	12	0.9947584681975385
1202	13	1.1030714567138347
1202	14	1.4111674703216295
1202	15	1.017079404438121
1202	16	1.2549266707411775
1202	17	1.3855729559429975
1202	18	1.6146553825261165
1202	19	1.417886060345971
1202	20	1.2523055834811032
1202	21	1.2559957384070017
1202	22	1.4754306289238315
1202	23	1.4714239024348714
1202	24	1.768022789805812
1202	25	1.5442881023306683
1203	1	0.5556115326087294
1203	2	0.7694979770515857
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2211	11	0.27222572350833474
2211	12	0.4075123383864323
2211	13	0.23634925452081745
2211	14	0.3902689965471424
2211	15	0.49145203507406165
2211	16	0.33728185047598913
2211	17	0.33653235492114675
2211	18	0.3298428012712691
2211	19	0.3856599779494587
2211	20	0.17305402029259653
2211	21	0.13470159676774074
2211	22	0.11169205012347305
2211	23	0.7974911860010891
2211	24	0.6152516580828262
2211	25	0.6088888474171412
2212	1	-0.18869595058581723
2212	2	-0.23859241928911246
2212	3	-0.24102582545603113
2212	4	-0.3692986762374808
2212	5	-0.3989332800000156
2212	6	-0.41026851109278084
2212	7	-0.5584681342065991
2212	8	-0.27242940405824356
2212	9	-0.1474408480020628
2212	10	-0.24054930895050575
2212	11	-0.42022822854908526
2212	12	-0.23418963060901632
2212	13	-0.3632788781210934
2212	14	-0.3409450854545426
2212	15	-0.46020982035318525
2212	16	-0.28957008054158706
2212	17	-0.4213792277785444
2212	18	-3.217818624177699
2212	19	-1.3211615282020333
2212	20	-0.6800848034471727
2212	21	-0.6535436767527258
2212	22	-0.4124637183793425
2212	23	-0.5927871894614825
2212	24	-0.8395862539397854
2212	25	-0.48245670434752697
2213	1	0.05290506976178477
2213	2	0.020606505493660165
2213	3	-0.23636741553088214
2213	4	-0.3415520232313618
2213	5	-0.22941580931919248
2213	6	-0.1765269249981074
2213	7	-0.011330718843176157
2213	8	-0.30069259204747567
2213	9	-0.12355710195603109
2213	10	-0.3027858219146964
2213	11	-0.2973385680264613
2213	12	-0.41044853137565696
2213	13	-0.29931809426726375
2213	14	-0.24081388165972584
2213	15	-0.45843686333167
2213	16	-1.217796771700577
2213	17	-0.6480405354169747
2213	18	-0.517432013645788
2213	19	-0.2925525620644791
2213	20	-1.0508472886858584
2213	21	-0.7321166968885322
2213	22	-0.6396284121915485
2213	23	-1.0888565839019506
2213	24	-0.7575952541220943
2213	25	-0.8750854818316469
2214	1	-1.2932900945942727
2214	2	-3.3447087854735926
2214	3	-1.581185882724089
2214	4	-1.1571836775017488
2214	5	-1.1580852274428146
2214	6	-1.2275850308790268
2214	7	-1.2410762119810954
2214	8	-1.0024040091214275
2214	9	-1.3110008333826784
2214	10	-1.4156742143194947
2214	11	-0.892825961638053
2214	12	-1.4133516846243737
2214	13	-1.3199237983967151
2214	14	-2.403457109214436
2214	15	-2.7347072035672824
2214	16	-1.897748391625214
2214	17	-2.4195975380609056
2214	18	-1.7976815240232256
2214	19	-4.020999724772221
2214	20	-2.320648934639099
2214	21	-2.2626608332454836
2214	22	-2.3809899087268462
2214	23	-2.3126593794922563
2214	24	-2.4909178156622573
2214	25	-2.4216452985096737
2215	1	-0.8564912040808323
2215	2	-2.7257267594568333
2215	3	-1.3194790941409664
2215	4	-0.802888011705317
2215	5	-0.9583780643494606
2215	6	-1.4549511957186994
2215	7	-1.9565790546642283
2215	8	-1.7519745344974247
2215	9	-1.474377578136398
2215	10	-3.2524389558530977
2215	11	-2.2500577291340775
2215	12	-2.3931789167011743
2215	13	-1.6226522057501604
2215	14	-3.052382607593092
2215	15	-2.889670843472352
2215	16	-2.4936472537995975
2215	17	-2.6658271479432933
2215	18	-1.4328017643785458
2215	19	-2.0764828304317575
2215	20	-2.348945774123848
2215	21	-1.8612895239058815
2215	22	-1.576748622150447
2215	23	-2.071724324415012
2215	24	-2.055967860427934
2215	25	-1.8037002812930716
2216	1	-1.9905581440961981
2216	2	-1.924016171560904
2216	3	-2.4950627073476532
2216	4	-3.55081385826702
2216	5	-2.456587463580828
2216	6	-2.5023133219123928
2216	7	-2.4492417614050304
2216	8	-2.8971898039558575
2216	9	-3.4091928421003885
2216	10	-2.846263247388414
2216	11	-3.5282395776423847
2216	12	-3.791567228836737
2216	13	-4.259420745336172
2216	14	-3.6479271023051787
2216	15	-3.3494048926535314
2216	16	-2.4826608142890336
2216	17	-2.5110319190848926
2216	18	-2.9023760232905857
2216	19	-3.0422077882667864
2216	20	-2.9764345865483612
2216	21	-3.263403512079087
2216	22	-4.024643366222087
2216	23	-3.8265135682516664
2216	24	-3.7545869393036533
2216	25	-4.086818546303949
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	79582.0
3	2.0
4	594.0
5	3703.0
6	4594.0
7	4109.0
8	3691.0
9	3518.0
10	3257.0
11	3033.0
12	2848.0
13	2814.0
14	2950.0
15	2951.0
16	3175.0
17	3261.0
18	3489.0
19	4418.0
20	5727.0
21	7271.0
22	9210.0
23	11155.0
24	12781.0
25	14567.0
26	16184.0
27	18011.0
28	20503.0
29	24264.0
30	29508.0
31	36351.0
32	45749.0
33	57788.0
34	74837.0
35	97815.0
36	132776.0
37	209415.0
38	510880.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.24260714525175	18.879403180319837	12.256861250644835	25.621128423783574
2	15.030938044791958	20.314823252095955	40.87000291919823	23.784235783913854
3	18.40745156238917	24.992497694746206	29.645495615925622	26.954555126939006
4	12.247428417875105	16.931929631200816	38.559000094119696	32.26164185680438
5	13.190050356615965	37.87809238253021	36.049962384296755	12.881894876557062
6	31.515140356942428	37.56398690497378	18.235185416270316	12.685687321813473
7	27.82378989200298	31.69328520131304	22.725332453246523	17.757592453437454
8	25.057657031020913	36.62179578977517	20.83256616408557	17.487981015118347
9	26.575632042823777	15.100018752450604	20.121515879916124	38.20283332480949
10	14.79200122743313	28.74382447706234	34.844235326207404	21.619938969297124
11	34.14134030326855	22.44469614160079	24.9607736573544	18.453189897776255
12	23.10327074603559	24.75695284984163	31.850308731422416	20.28946767270037
13	28.799982543316098	21.995012570858112	25.80111518206435	23.403889703761436
14	20.960560512229833	21.858763084153896	27.717436150283724	29.46324025333254
15	23.020902736907573	30.542505505117084	23.397207921912923	23.03938383606242
16	22.43522863810003	28.047291924940637	27.252524095342135	22.264955341617192
17	21.93451906041971	28.360821634950444	27.615071035881407	22.08958826874844
18	21.95327661456073	27.417508031520132	29.111565116044208	21.517650237874932
19	23.405745412984803	27.08545016373186	27.4941319756523	22.014672447631035
20	23.54834904132494	27.188497945038737	27.764037120913006	21.499115892723317
21	23.028746551082172	26.775195342068546	27.141125012104457	23.054933094744825
22	23.279242763849215	27.822400546610925	26.973776177283053	21.92458051225681
23	22.486852585294535	27.58324877527195	27.461935233980338	22.467963405453176
24	23.029505424443066	26.918739047125488	27.951326618979756	22.10042890945169
25	22.83593702585099	27.104497072167145	27.65119407982445	22.408371822157424
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	223.0
1	223.0
2	336.0
3	449.0
4	449.0
5	449.0
6	811.0
7	1173.0
8	1173.0
9	1173.0
10	1889.5
11	2606.0
12	2606.0
13	2606.0
14	4552.0
15	6498.0
16	6498.0
17	6498.0
18	11787.0
19	17076.0
20	17076.0
21	17076.0
22	26960.5
23	36845.0
24	36845.0
25	36845.0
26	52553.0
27	68261.0
28	68261.0
29	68261.0
30	87641.0
31	107021.0
32	107021.0
33	107021.0
34	123075.0
35	139129.0
36	139129.0
37	139129.0
38	151179.0
39	163229.0
40	163229.0
41	163229.0
42	171873.5
43	180518.0
44	180518.0
45	180518.0
46	185222.0
47	189926.0
48	189926.0
49	189926.0
50	183844.5
51	177763.0
52	177763.0
53	177763.0
54	159900.5
55	142038.0
56	142038.0
57	142038.0
58	122501.5
59	102965.0
60	102965.0
61	102965.0
62	84566.0
63	66167.0
64	66167.0
65	66167.0
66	51557.5
67	36948.0
68	36948.0
69	36948.0
70	27113.0
71	17278.0
72	17278.0
73	17278.0
74	12081.5
75	6885.0
76	6885.0
77	6885.0
78	4743.5
79	2602.0
80	2602.0
81	2602.0
82	1722.0
83	842.0
84	842.0
85	842.0
86	546.5
87	251.0
88	251.0
89	251.0
90	159.5
91	68.0
92	68.0
93	68.0
94	40.0
95	12.0
96	12.0
97	12.0
98	10.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3522679936541311
2	0.042610314695922566
3	0.038519724485114
4	0.03838337147808705
5	0.043496609241597754
6	0.01840765594863855
7	0.017657714409990314
8	0.023929952733230113
9	0.02086201007512369
10	0.02086201007512369
11	0.019907539025935023
12	0.02018024503998893
13	0.020043892032961978
14	0.027816013433498255
15	0.028497778468633012
16	0.021475598606744972
17	0.0231118346910684
18	0.08897033708508632
19	0.024475364761337923
20	0.022498246159447115
21	0.025838894831607444
22	0.019771186018908073
23	0.022702775669987546
24	0.018475832452152025
25	0.023043658187554925
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1466781.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.01269946670564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.27236522458453	63.21497605940215
2	10.940679855462283	16.413798599184652
3	2.689085232237715	6.05146627098612
4	0.8937770225544577	2.681785087324981
5	0.3984071690997879	1.494279862053168
6	0.21007947421850395	0.9455177078205703
7	0.14108378969390542	0.740815314115298
8	0.08446997396730806	0.5069056616936103
9	0.06014339531002203	0.406036659356816
>10	0.28110773227055913	3.538428702363773
>50	0.016604904457365663	0.8628906980577989
>100	0.010284967113125989	1.6192266461123028
>500	0.0011831603522417177	0.6085659929594106
>1k	7.280986783025955E-4	0.9153067385693088
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2140	0.14589771751883887	No Hit
GTCCTACAGTGGACATTTCTAAATT	2049	0.13969365569911255	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1957	0.13342141737587274	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1840	0.12544476646479605	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1664	0.11344570184642425	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	425	0.0	12.740101	4
TTCGTGA	55	0.0030773098	12.086178	10
TAGGCTA	140	6.8030204E-10	11.534741	5
ATAGGAC	165	7.2759576E-12	11.514573	3
GTTCTAG	170	1.0913936E-11	11.200746	1
TGTAGGA	1215	0.0	10.945952	2
GTGTTAG	140	8.067218E-9	10.880723	1
GGACGTG	985	0.0	10.604262	6
GTAGGAC	1095	0.0	10.583942	3
AGGACGT	1040	0.0	10.503921	5
AATCCCG	100	2.410312E-5	10.445198	19
ATACTCT	155	3.6870915E-9	10.414568	6
CTGTAGG	1180	0.0	10.246783	1
GACGTGG	510	0.0	10.240391	7
ATAAGAC	130	3.8418875E-7	10.230255	3
GTCTAAT	140	9.379437E-8	10.200678	1
TCGGGCC	75	0.0026432793	10.135244	13
ACTACTC	85	6.6203764E-4	10.054202	8
GTCCTAT	95	1.608078E-4	10.02172	1
GATATAC	200	2.3646862E-11	9.996665	1
>>END_MODULE