FastQCFastQC Report
Thu 26 May 2016
SRR1512974_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512974_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466781
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27670.1886443852217884No Hit
GTCCTACAGTGGACATTTCTAAATT20090.13696659555857352No Hit
TATCAACGCAGAGTACTTTTTTTTT19090.1301489452072259No Hit
CTGTAGGACGTGGAATATGGCAAGA19090.1301489452072259No Hit
GGTATCAACGCAGAGTACTTTTTTT17030.11610458548344982No Hit
GTCCTAAAGTGTGTATTTCTCATTT16880.11508193793074767No Hit
CTTTAGGACGTGAAATATGGCGAGG16570.1129684663218299No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG402.732775E-416.6473944
TAGGACC4100.012.9930874
TAAACGT550.00303752112.1071964
TGCACCG802.8268838E-511.8909955
CCCTTAC657.994456E-411.6972661
GTCCTAA5550.011.4733311
GGCGAGG5100.010.97911519
GACGTGG5200.010.9699327
TCTACAC2350.010.9213863
GTAGGAC10700.010.8382193
ACTAGAC1252.2207132E-710.6463293
AGGACGT10800.010.4816925
CTAGTAC1002.3824294E-510.4562153
GGACGTG10600.010.4077416
TAGACAG2300.010.3399965
TGTAGGA11300.010.1779212
TGGCGAG10700.010.1112918
TAGGACA3500.010.0563864
ATAGGAC2600.09.8712533
TTACACC1553.976129E-89.8196614
Produced by FastQC (version 0.11.5)