##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512974_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1466781 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.642995102881752 31.0 31.0 34.0 27.0 34.0 2 30.757743657710318 31.0 31.0 34.0 27.0 34.0 3 30.868277541091683 31.0 31.0 34.0 27.0 34.0 4 34.36945665371995 37.0 35.0 37.0 31.0 37.0 5 34.05681557096799 37.0 35.0 37.0 30.0 37.0 6 33.94184271544287 37.0 35.0 37.0 30.0 37.0 7 33.810350693116426 37.0 35.0 37.0 29.0 37.0 8 33.694732206103026 37.0 35.0 37.0 28.0 37.0 9 35.171034394364256 39.0 35.0 39.0 28.0 39.0 10 35.05821046222987 39.0 35.0 39.0 28.0 39.0 11 35.10610786477327 39.0 35.0 39.0 28.0 39.0 12 34.96539497034663 39.0 35.0 39.0 27.0 39.0 13 34.96009152013831 39.0 35.0 39.0 27.0 39.0 14 36.040879995036754 40.0 36.0 41.0 27.0 41.0 15 36.05792548444519 40.0 36.0 41.0 27.0 41.0 16 36.03648465585524 40.0 36.0 41.0 27.0 41.0 17 36.00179985969275 40.0 36.0 41.0 27.0 41.0 18 35.926530954518775 40.0 36.0 41.0 27.0 41.0 19 35.90582575040173 40.0 36.0 41.0 27.0 41.0 20 35.88545120232673 40.0 36.0 41.0 27.0 41.0 21 35.82949533706804 40.0 36.0 41.0 27.0 41.0 22 35.80377438758752 40.0 36.0 41.0 27.0 41.0 23 35.69561986417877 40.0 36.0 41.0 27.0 41.0 24 35.693766826813274 40.0 36.0 41.0 27.0 41.0 25 35.5951120174041 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6399539978884654 1101 2 0.6711360392230397 1101 3 0.5311311992423171 1101 4 0.42111179518243347 1101 5 0.8014272341053683 1101 6 0.725779344924284 1101 7 0.7428334617635883 1101 8 0.6525171463515207 1101 9 0.5991052324536739 1101 10 0.76522124172903 1101 11 0.7791916615319607 1101 12 0.8742941913947888 1101 13 0.9954856280993454 1101 14 0.9978382416284646 1101 15 0.920766085792053 1101 16 1.1226826595928543 1101 17 1.0471451872838742 1101 18 0.6834714098477193 1101 19 0.8795266800379977 1101 20 0.8887368546317447 1101 21 0.9088201087785919 1101 22 0.7475023260386209 1101 23 0.928436010395167 1101 24 0.8773755098319995 1101 25 0.8722774758313818 1102 1 0.8122989596226162 1102 2 0.8554036059537218 1102 3 0.7746314876550713 1102 4 0.6546489093097136 1102 5 0.8551252879563123 1102 6 0.8117433346418039 1102 7 0.7286124348906213 1102 8 0.6812035980560935 1102 9 0.9859142290784391 1102 10 1.1625493890380625 1102 11 1.0736363762427104 1102 12 1.0860567215198316 1102 13 1.1081832024206548 1102 14 1.2047085655387662 1102 15 1.2029298996048254 1102 16 1.132120745057236 1102 17 1.1516919159419103 1102 18 1.164880949012776 1102 19 1.2482343978398234 1102 20 1.2831909128347547 1102 21 1.2554756404734917 1102 22 1.1465694439728011 1102 23 1.2857346291799132 1102 24 1.2543348440193753 1102 25 1.4198960821993154 1103 1 0.6573996473378578 1103 2 0.4790462831491915 1103 3 0.627844113185315 1103 4 0.6483970552493048 1103 5 0.7429013641395628 1103 6 0.7795184470900978 1103 7 0.7452530505967871 1103 8 0.7564216054437196 1103 9 0.8522513435656336 1103 10 0.9651124722671227 1103 11 0.9199368386849045 1103 12 0.8536222969072469 1103 13 0.9373175124391295 1103 14 1.0945792594128747 1103 15 0.8480796775693236 1103 16 0.9897518694799317 1103 17 1.1427054639472018 1103 18 1.2083235608549714 1103 19 1.2845227834783657 1103 20 1.2143365678206521 1103 21 0.9728554710067243 1103 22 1.2009771761388066 1103 23 1.2591483440318214 1103 24 1.218732743077176 1103 25 1.2657726518449763 1104 1 0.49282927794821063 1104 2 0.5499609904880494 1104 3 0.4447058775276105 1104 4 0.4512080198288899 1104 5 0.5179503183804215 1104 6 0.6060745043590643 1104 7 0.6280585729716393 1104 8 0.6532442795412408 1104 9 0.6307530621758488 1104 10 0.7819321765593656 1104 11 0.6215791190564701 1104 12 0.6029120306707867 1104 13 0.7386755765942397 1104 14 0.7879951426044371 1104 15 0.9794635702525625 1104 16 0.9289313912827666 1104 17 0.7439675946142472 1104 18 0.8902859438091681 1104 19 0.791475858360613 1104 20 0.8090686014131023 1104 21 0.7280837180974515 1104 22 0.8313583938028586 1104 23 0.8225555153069308 1104 24 0.9606612366032152 1104 25 0.9637454671545456 1105 1 0.18955783674573468 1105 2 0.31210154744666596 1105 3 0.28945937554839674 1105 4 0.19422514044174477 1105 5 0.14515991485400548 1105 6 0.22472858887966396 1105 7 0.40960082666732234 1105 8 0.43208100274831907 1105 9 0.48612876164883545 1105 10 0.4751669210419678 1105 11 0.5095950519063379 1105 12 0.4429702012046022 1105 13 0.6015310167024879 1105 14 0.6906684144273925 1105 15 0.6223880037402765 1105 16 0.6747423330675488 1105 17 0.5008805470565107 1105 18 0.6894885052521573 1105 19 0.586654499541666 1105 20 0.6127028847578018 1105 21 0.5642634308885803 1105 22 0.6480812281798549 1105 23 0.6241815474530625 1105 24 0.48624120055239217 1105 25 0.5708951670838189 1106 1 0.4987439100909512 1106 2 0.5268104158991456 1106 3 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2216 17 -2.267415192206599 2216 18 -1.9307629625733895 2216 19 -2.041192896044663 2216 20 -2.7126709523276773 2216 21 -2.5062522988351077 2216 22 -2.88449103814461 2216 23 -1.9769509354974346 2216 24 -2.3356131892479084 2216 25 -2.0691992473202987 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32059.0 3 0.0 4 531.0 5 4473.0 6 5235.0 7 4146.0 8 3364.0 9 3003.0 10 2619.0 11 2447.0 12 2218.0 13 2084.0 14 2108.0 15 2208.0 16 2233.0 17 2213.0 18 2609.0 19 3023.0 20 3928.0 21 5125.0 22 6511.0 23 8011.0 24 9765.0 25 11174.0 26 12968.0 27 14375.0 28 16540.0 29 20046.0 30 25186.0 31 32470.0 32 41240.0 33 53205.0 34 69493.0 35 90999.0 36 124834.0 37 194469.0 38 649869.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.9019967714336 19.366349495724126 13.220883884868723 27.51076984797355 2 13.958359041626641 20.65467347797245 39.79936963832948 25.587597842071425 3 16.78410069396863 25.059637396448416 30.255232376203402 27.901029533379557 4 11.486221539665967 17.423415301459443 37.899307343306624 33.191055815567964 5 12.7129184089357 38.059137881278055 36.26300604259494 12.96493766719131 6 31.583967382405277 37.944562096782995 17.87042747824948 12.60104304256225 7 27.402497584050163 31.761846338088397 22.849395449898623 17.98626062796282 8 24.913392831559804 36.959464161210086 20.646821493040726 17.480321514189384 9 26.258924350970247 14.91310459983916 19.93822954245798 38.88974150673261 10 14.423368305527573 28.994638142897315 34.96291200165989 21.61908154991523 11 34.130026746590545 22.369367483700124 24.824967666146915 18.675638103562417 12 22.816952307571672 24.883385552150063 31.95948410763781 20.340178032640456 13 28.724348633586196 21.924733719419155 25.869545355993033 23.481372291001616 14 20.831543564673716 21.864223035856735 27.717414278603915 29.58681912086563 15 22.881074539349207 30.612439702047144 23.409821535104914 23.09666422349873 16 22.390090126335068 28.061564394608375 27.1847811485465 22.36356433051005 17 21.82667796013732 28.381664750322916 27.662456166194062 22.129201123345705 18 21.75626956682993 27.508644857264407 29.096285165752185 21.63880041015348 19 23.262720617862964 27.067503083383276 27.56636688477044 22.10340941398332 20 23.454959368218237 27.267099335996438 27.751858071369305 21.526083224416023 21 22.9476969604828 26.753519105468516 27.292727806983873 23.006056127064813 22 23.19547741879623 27.6768797898516 27.19036960729438 21.937273184057794 23 22.290391197043867 27.49171654917574 27.626297058863635 22.591595194916756 24 22.967588868132918 27.008276633169253 27.902821145638747 22.121313353059083 25 22.667021196811955 27.137850865530805 27.71464414036667 22.48048379729057 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 127.0 1 127.0 2 203.0 3 279.0 4 279.0 5 279.0 6 740.5 7 1202.0 8 1202.0 9 1202.0 10 1757.5 11 2313.0 12 2313.0 13 2313.0 14 4366.0 15 6419.0 16 6419.0 17 6419.0 18 11882.0 19 17345.0 20 17345.0 21 17345.0 22 27550.5 23 37756.0 24 37756.0 25 37756.0 26 53514.5 27 69273.0 28 69273.0 29 69273.0 30 88446.5 31 107620.0 32 107620.0 33 107620.0 34 123362.5 35 139105.0 36 139105.0 37 139105.0 38 151818.5 39 164532.0 40 164532.0 41 164532.0 42 172703.5 43 180875.0 44 180875.0 45 180875.0 46 186003.0 47 191131.0 48 191131.0 49 191131.0 50 184678.0 51 178225.0 52 178225.0 53 178225.0 54 159844.5 55 141464.0 56 141464.0 57 141464.0 58 121750.5 59 102037.0 60 102037.0 61 102037.0 62 83432.0 63 64827.0 64 64827.0 65 64827.0 66 50202.0 67 35577.0 68 35577.0 69 35577.0 70 26061.0 71 16545.0 72 16545.0 73 16545.0 74 11547.5 75 6550.0 76 6550.0 77 6550.0 78 4521.5 79 2493.0 80 2493.0 81 2493.0 82 1641.0 83 789.0 84 789.0 85 789.0 86 517.5 87 246.0 88 246.0 89 246.0 90 147.0 91 48.0 92 48.0 93 48.0 94 25.5 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07601680141752586 2 2.7270601405390447E-4 3 0.0 4 0.02713424839836349 5 0.06954003358374564 6 0.07288068225590597 7 0.03299742770052244 8 0.0659266788975314 9 0.04826896448754108 10 0.11024140618129087 11 0.0035451781827007577 12 0.013362594688641318 13 0.006476767833780231 14 0.007567591889995848 15 0.007226709372428467 16 0.019362126997827217 17 0.030474897070523824 18 8.862945456751894E-4 19 0.0032724721686468532 20 0.00490870825297028 21 1.3635300702695224E-4 22 5.454120281078089E-4 23 6.817650351347612E-5 24 6.817650351347612E-5 25 0.003477001679187282 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1466781.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.36458396572368 #Duplication Level Percentage of deduplicated Percentage of total 1 82.90498075737767 60.8228942195134 2 11.84651130075922 17.382287460508884 3 2.9548756185738974 6.503496612814032 4 0.967227855253635 2.8384107680296853 5 0.47036776727234825 1.7254167778411087 6 0.22464139543270228 0.9888433510439893 7 0.14228951961296193 0.7307307986361341 8 0.09005994777282048 0.5285768480262222 9 0.06255725978762952 0.41305386045196224 >10 0.30637660746154904 3.8037162555238875 >50 0.016607781012256975 0.8429888872390048 >100 0.011455400713024618 1.7224639817917264 >500 0.001303774799266337 0.6894379920310112 >1k 7.450141710093355E-4 1.007682186548943 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2767 0.1886443852217884 No Hit GTCCTACAGTGGACATTTCTAAATT 2009 0.13696659555857352 No Hit TATCAACGCAGAGTACTTTTTTTTT 1909 0.1301489452072259 No Hit CTGTAGGACGTGGAATATGGCAAGA 1909 0.1301489452072259 No Hit GGTATCAACGCAGAGTACTTTTTTT 1703 0.11610458548344982 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1688 0.11508193793074767 No Hit CTTTAGGACGTGAAATATGGCGAGG 1657 0.1129684663218299 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGACG 40 2.732775E-4 16.647394 4 TAGGACC 410 0.0 12.993087 4 TAAACGT 55 0.003037521 12.107196 4 TGCACCG 80 2.8268838E-5 11.890995 5 CCCTTAC 65 7.994456E-4 11.697266 1 GTCCTAA 555 0.0 11.473331 1 GGCGAGG 510 0.0 10.979115 19 GACGTGG 520 0.0 10.969932 7 TCTACAC 235 0.0 10.921386 3 GTAGGAC 1070 0.0 10.838219 3 ACTAGAC 125 2.2207132E-7 10.646329 3 AGGACGT 1080 0.0 10.481692 5 CTAGTAC 100 2.3824294E-5 10.456215 3 GGACGTG 1060 0.0 10.407741 6 TAGACAG 230 0.0 10.339996 5 TGTAGGA 1130 0.0 10.177921 2 TGGCGAG 1070 0.0 10.11129 18 TAGGACA 350 0.0 10.056386 4 ATAGGAC 260 0.0 9.871253 3 TTACACC 155 3.976129E-8 9.819661 4 >>END_MODULE