FastQCFastQC Report
Thu 26 May 2016
SRR1512973_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512973_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1952205
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30100.1541846271267618No Hit
GTCCTACAGTGGACATTTCTAAATT27900.14291531883178252No Hit
GTCCTAAAGTGTGTATTTCTCATTT27590.1413273708447627No Hit
CTGTAGGACGTGGAATATGGCAAGA23990.12288668454388754No Hit
CTTTAGGACGTGAAATATGGCGAGG23090.11827651296866877No Hit
GTCCTACAGTGTGCATTTCTCATTT19540.10009194731086131No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC551.9708797E-413.80962858
CTCGAAC1700.012.29753618
GCGTGCG1251.4242687E-912.1534089
TCGGGGC550.00307314912.088693
TAGGACC6450.012.0753034
CGTGCGC1302.6393536E-911.68596810
TCGAACT1900.011.49376619
CGCCACC1701.0913936E-1111.17957814
GCCTCGA1900.011.00305816
GGTATCA9750.010.9447651
GTCCTAC14400.010.9172811
AGTCGTC1158.8896013E-710.7324838
GTAGGAC15300.010.6777793
AGGACGT13800.010.5989615
GGACGTG13600.010.5409956
TGTAGGA16000.010.5074472
GACGTGG6850.010.3947557
TAGGACT3200.010.3887194
CTAGTAC1106.059865E-610.3617343
GGCGTGC1659.385985E-1010.3572228
Produced by FastQC (version 0.11.5)