##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512973_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1952205
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.973300447442764	31.0	30.0	33.0	23.0	34.0
2	29.113362070069485	31.0	30.0	34.0	23.0	34.0
3	29.388863874439416	31.0	30.0	34.0	23.0	34.0
4	32.839185433906785	35.0	33.0	37.0	26.0	37.0
5	33.024829359621556	37.0	35.0	37.0	26.0	37.0
6	33.025089578194915	37.0	35.0	37.0	26.0	37.0
7	32.82889348198575	37.0	35.0	37.0	25.0	37.0
8	32.88535117981974	37.0	35.0	37.0	26.0	37.0
9	34.22066483796527	39.0	35.0	39.0	25.0	39.0
10	34.07916381732451	39.0	35.0	39.0	25.0	39.0
11	34.17562909632954	39.0	35.0	39.0	25.0	39.0
12	34.06069342102904	39.0	35.0	39.0	25.0	39.0
13	34.07203341862151	39.0	35.0	39.0	25.0	39.0
14	35.09494238566134	40.0	35.0	41.0	25.0	41.0
15	35.02496612804495	40.0	35.0	41.0	24.0	41.0
16	34.92218286501674	39.0	35.0	41.0	22.0	41.0
17	34.87967861981708	39.0	35.0	41.0	22.0	41.0
18	34.5642399235736	39.0	34.0	41.0	18.0	41.0
19	34.798880240548506	39.0	34.0	41.0	19.0	41.0
20	34.866229724849596	40.0	35.0	41.0	22.0	41.0
21	34.817602659556755	39.0	35.0	41.0	21.0	41.0
22	34.743125337759096	39.0	35.0	41.0	19.0	41.0
23	34.470038751053295	39.0	34.0	41.0	18.0	41.0
24	34.48024106074926	39.0	34.0	41.0	18.0	41.0
25	34.41308366693047	39.0	34.0	41.0	17.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8114857288125528
1101	2	0.9797978436844481
1101	3	1.0178686519265696
1101	4	0.9823743616439202
1101	5	0.9503200496905677
1101	6	1.0951349042592327
1101	7	1.1792065081526815
1101	8	0.9031859426082747
1101	9	0.8524696043270836
1101	10	1.008097959443397
1101	11	0.9535673669779996
1101	12	1.2146007955065912
1101	13	1.0257963196736668
1101	14	0.9049818035588899
1101	15	0.8680627280188276
1101	16	1.2820179155031255
1101	17	1.2159243441372425
1101	18	1.4799973787562308
1101	19	0.9910125702260686
1101	20	1.1844129674802275
1101	21	1.242550064008455
1101	22	1.2356297361830926
1101	23	1.3868274766820008
1101	24	1.0113252226575753
1101	25	1.2437499361102908
1102	1	0.6289412279395599
1102	2	0.6504554012862442
1102	3	0.7787216487171449
1102	4	0.8829986175477913
1102	5	0.8665990015202283
1102	6	0.8659347004402846
1102	7	0.9663253437765604
1102	8	1.032379384661148
1102	9	1.0339155597042549
1102	10	1.1428507806695478
1102	11	1.0537854339339745
1102	12	0.9254848239736262
1102	13	0.9267871598955466
1102	14	0.892676113774229
1102	15	1.1712265100677897
1102	16	1.2358005198939281
1102	17	0.9526653660815896
1102	18	1.3206621569582282
1102	19	1.324541994775629
1102	20	1.230842392754262
1102	21	1.0401976938886932
1102	22	1.2641948169741255
1102	23	1.4002623814014754
1102	24	1.3421484918265776
1102	25	1.2405424440734834
1103	1	0.19419645918509332
1103	2	0.38598748379334324
1103	3	0.34582635111465976
1103	4	0.5278782017484502
1103	5	0.1340358409004807
1103	6	0.4510091993877552
1103	7	0.27012607567544933
1103	8	0.36181031390505325
1103	9	0.6067168398815781
1103	10	0.5508282906870718
1103	11	0.5936126381718054
1103	12	0.6938643030973637
1103	13	0.6155669409833493
1103	14	0.8820830690443344
1103	15	0.7979756885306628
1103	16	0.897639166362346
1103	17	0.7625710081837411
1103	18	0.9983987649344428
1103	19	1.0073807621705555
1103	20	0.9063385730028557
1103	21	0.6125989773533433
1103	22	0.7793342288016802
1103	23	1.1685992427363345
1103	24	0.9999993812234322
1103	25	0.9554532695788751
1104	1	0.2639543850077324
1104	2	0.3343079211381763
1104	3	0.4500203944332455
1104	4	0.624640098491561
1104	5	0.3536087524084195
1104	6	0.42853326212029685
1104	7	0.658385733672489
1104	8	0.6431514288577276
1104	9	0.7233482585667659
1104	10	0.677392330714099
1104	11	0.7744486618478987
1104	12	0.6692630779341258
1104	13	0.7054436266352724
1104	14	0.7887441163893598
1104	15	0.9636868469166586
1104	16	0.7563683561553205
1104	17	0.9424126247348923
1104	18	1.0635500112742307
1104	19	0.9203331289358445
1104	20	0.8927525058980663
1104	21	0.9668259246992719
1104	22	0.7501515370833829
1104	23	1.1957852556217645
1104	24	1.0356309153329377
1104	25	0.7457384971856911
1105	1	0.3745978781791024
1105	2	0.5288102637605618
1105	3	0.5307004536095903
1105	4	0.577814922039849
1105	5	0.31096802152105596
1105	6	0.17630964152685635
1105	7	0.4532863716843636
1105	8	0.4314740625476787
1105	9	0.4176078645806811
1105	10	0.5432211917136982
1105	11	0.5485566667918818
1105	12	0.5723751100069094
1105	13	0.5920078736289014
1105	14	0.7172326043183403
1105	15	0.8764923411932983
1105	16	0.7393048966470275
1105	17	0.7550429519808048
1105	18	0.8221913894592205
1105	19	0.7753114156165708
1105	20	0.8856099192138274
1105	21	0.8212806485745432
1105	22	0.6027322081272715
1105	23	0.763653690126155
1105	24	0.8900802300358848
1105	25	0.9348350482072618
1106	1	0.5193153889109006
1106	2	0.6439981326454998
1106	3	0.7605436558705208
1106	4	0.7155704623814998
1106	5	0.7308056485954211
1106	6	0.8578347793943806
1106	7	0.5263254795142629
1106	8	0.7711447145376908
1106	9	0.8988893510165852
1106	10	0.9568774621768696
1106	11	0.8788154659002032
1106	12	0.9595045376123252
1106	13	0.9970438625100826
1106	14	1.1935174612876267
1106	15	1.2056597712339112
1106	16	1.1386743132423263
1106	17	1.0170267541353155
1106	18	1.2894126235565722
1106	19	0.9211349784938889
1106	20	1.0054560493243088
1106	21	0.9806882499381828
1106	22	1.073474751096093
1106	23	1.24307721934197
1106	24	1.1173446893611256
1106	25	1.2253162937530249
1107	1	0.4694528982556285
1107	2	0.6204863934572273
1107	3	0.7073493633052053
1107	4	0.7207831099262023
1107	5	0.6683320783972349
1107	6	0.6641053415145635
1107	7	0.6566616432181718
1107	8	0.7014435630802112
1107	9	0.9865376925956681
1107	10	0.7359513066712466
1107	11	0.6960015627300749
1107	12	0.8291377737760683
1107	13	0.9357109972454438
1107	14	1.1883298888513778
1107	15	1.1955122148457349
1107	16	1.208703778619764
1107	17	1.1639902647524991
1107	18	1.587346349619935
1107	19	1.3301012847731641
1107	20	1.290830546069003
1107	21	1.2021558577855913
1107	22	1.2186598415216139
1107	23	1.2560580504840644
1107	24	1.2553098485604366
1107	25	1.2496805866285925
1108	1	0.4200942112203663
1108	2	0.43700348454390436
1108	3	0.4003871580382281
1108	4	0.5655674350967104
1108	5	0.5601603764467811
1108	6	0.5585650798715847
1108	7	0.5708476716436621
1108	8	0.6447591987736416
1108	9	0.7200612428052295
1108	10	0.771960247756418
1108	11	0.6165752154627384
1108	12	0.6756432912919692
1108	13	0.6990059694320792
1108	14	0.8256226266029643
1108	15	0.8863492332129184
1108	16	0.7347319783274173
1108	17	0.7827038255655907
1108	18	0.9570504374987934
1108	19	0.8587368828692092
1108	20	0.9688331517949109
1108	21	0.8858336107734814
1108	22	0.8124599114313327
1108	23	1.1299011115262871
1108	24	1.126956105379982
1108	25	1.1683432203852888
1109	1	0.09222353555176355
1109	2	0.10157921004224235
1109	3	0.2818661633681465
1109	4	0.37784162695680834
1109	5	0.2682671630154658
1109	6	0.24395736136949608
1109	7	0.3023177923729321
1109	8	0.4063246907314948
1109	9	0.7018032829627785
1109	10	0.6687526325773874
1109	11	0.33312152850119503
1109	12	0.3850602926611657
1109	13	0.44602677923493417
1109	14	0.3296084568256319
1109	15	0.5430793920636745
1109	16	0.5782012481609655
1109	17	0.41218305712571635
1109	18	-1.0637536673977408
1109	19	0.28941621083042435
1109	20	0.4546783579936218
1109	21	0.4766983841971708
1109	22	0.6380141543451785
1109	23	0.14447101244083171
1109	24	0.5108219238521841
1109	25	0.49790724606748427
1110	1	0.22931551799479166
1110	2	0.12637172724462786
1110	3	0.23579827384674346
1110	4	0.12386926577381985
1110	5	0.29243675264994806
1110	6	0.2155326972712075
1110	7	0.27967197940454014
1110	8	0.1466884804311448
1110	9	0.4129853119816218
1110	10	0.5953909320809601
1110	11	0.44588100179198165
1110	12	-0.07809114582234145
1110	13	0.15697880422870725
1110	14	0.3692458171461013
1110	15	0.41918389055906147
1110	16	0.6221128499473352
1110	17	0.20454692349338188
1110	18	0.5922369791052446
1110	19	0.4938802056169962
1110	20	0.5072438612232588
1110	21	0.45950558332314984
1110	22	0.47742342734078846
1110	23	0.3159773471350249
1110	24	0.638637077284038
1110	25	0.6153626348701167
1111	1	0.14350938820748027
1111	2	0.35685145780233896
1111	3	0.3559215330487895
1111	4	0.32979184691409813
1111	5	0.1619036733112722
1111	6	0.4190607722219184
1111	7	0.3376859936713146
1111	8	0.1267755218760911
1111	9	0.44667673817649245
1111	10	0.4437875598438623
1111	11	0.234468818712827
1111	12	0.40507675677838506
1111	13	0.2645353929269376
1111	14	0.24719222328257473
1111	15	0.527663252255131
1111	16	0.3650639411217611
1111	17	0.3335531879523259
1111	18	0.5871075742721672
1111	19	0.3449628392891171
1111	20	0.22375636105306285
1111	21	0.43491191907884286
1111	22	0.2864663551614939
1111	23	0.5540905331960104
1111	24	0.6497846207026399
1111	25	0.11003827799524402
1112	1	-0.11688643204876925
1112	2	0.15673822563948292
1112	3	0.16771860132040217
1112	4	0.31018852871941505
1112	5	0.24879766585272023
1112	6	0.2612274450425005
1112	7	-0.01033613583181392
1112	8	0.30620419176741365
1112	9	0.5637735639835739
1112	10	0.5283399851044805
1112	11	0.4282035108321125
1112	12	0.45685031706835133
1112	13	0.12865125896836105
1112	14	0.32440794286065966
1112	15	0.3329654713593655
1112	16	0.5051579730483056
1112	17	0.5502112391283376
1112	18	0.4299567170308478
1112	19	0.5132147562357829
1112	20	0.4258271557705342
1112	21	0.5255474961320985
1112	22	0.41231775025558903
1112	23	0.6343899830353976
1112	24	0.43056838419511223
1112	25	0.6289234335048519
1113	1	-0.13480511526870842
1113	2	-0.014353168061855825
1113	3	-0.30916526859145677
1113	4	-0.03325095130209377
1113	5	-0.10972095657836434
1113	6	0.08814755446243794
1113	7	-0.062075632219553256
1113	8	0.15607092389900146
1113	9	0.009905288750360342
1113	10	-0.16973113785266492
1113	11	-0.07212381375697419
1113	12	-0.003302590275403361
1113	13	-0.05769462960099503
1113	14	0.0958640696755566
1113	15	-0.27566537664512225
1113	16	-0.22124543112742145
1113	17	-0.22623191160463563
1113	18	-0.2448720220680798
1113	19	-0.18958503801733428
1113	20	-0.3615042136152624
1113	21	-0.19485768494853772
1113	22	0.0047472001164763356
1113	23	-0.420074490556523
1113	24	0.027810674532027235
1113	25	0.018951723701476908
1114	1	-1.1836840858382445
1114	2	-0.982165912975713
1114	3	-0.9595363516301809
1114	4	-1.1009901627577534
1114	5	-1.1262555476659202
1114	6	-1.5755365951397238
1114	7	-1.673999248509638
1114	8	-1.2588964082691838
1114	9	-1.4350292522395662
1114	10	-1.4897352747026957
1114	11	-2.2295160611481997
1114	12	-1.6910615940915577
1114	13	-1.499485449209665
1114	14	-1.910730315973744
1114	15	-1.7547140339716165
1114	16	-2.449784382986252
1114	17	-4.184496977424196
1114	18	-3.3785011022261884
1114	19	-2.4009328161683072
1114	20	-1.5615195844800738
1114	21	-2.526569486926256
1114	22	-2.7054541202597875
1114	23	-2.6511508685735308
1114	24	-2.196037411985067
1114	25	-1.9837235658305872
1115	1	-0.7703915520172266
1115	2	-0.5065924404396149
1115	3	-0.6882164259126533
1115	4	-0.5575900737166748
1115	5	-0.4839931325067255
1115	6	-0.5797883536068582
1115	7	-1.5594111011381848
1115	8	-1.058223141017006
1115	9	-0.8106606108877941
1115	10	-0.5494978381068165
1115	11	-1.2161712886406164
1115	12	-1.0548637610087468
1115	13	-0.9029048712709553
1115	14	-0.9368009031459934
1115	15	-2.054012529591567
1115	16	-0.9773599149502061
1115	17	-1.0711555933719623
1115	18	-1.086802723696195
1115	19	-1.0820858510477507
1115	20	-0.8327355034432813
1115	21	-1.4942398579903582
1115	22	-1.6527636396544594
1115	23	-1.0112643253533093
1115	24	-1.0253419032885063
1115	25	-1.2818348784711233
1116	1	-1.2112958128619482
1116	2	-2.2127143388037815
1116	3	-1.8055972371063866
1116	4	-1.4037808219759533
1116	5	-2.027952932074463
1116	6	-1.792714445293221
1116	7	-1.3662844040575237
1116	8	-2.1030514996135743
1116	9	-2.0025299172813718
1116	10	-3.1299712027775257
1116	11	-1.7229170490048915
1116	12	-1.6741721384192871
1116	13	-2.1833327520157795
1116	14	-1.9486299701520053
1116	15	-1.7523886344073105
1116	16	-1.687020253543949
1116	17	-2.0138887697298458
1116	18	-1.9701750484045348
1116	19	-2.115975163154772
1116	20	-2.4764327875371066
1116	21	-2.9255154855314665
1116	22	-2.1370599625621978
1116	23	-2.1856228613263724
1116	24	-2.782528435718074
1116	25	-2.4318735812883148
1201	1	0.07250061464307223
1201	2	0.1239899810186138
1201	3	0.3820429922202244
1201	4	0.4704636266296802
1201	5	0.37383772262146664
1201	6	0.41487345846180546
1201	7	0.27135922029489024
1201	8	-0.1725418788274098
1201	9	0.20385812952865479
1201	10	0.41980388203485575
1201	11	0.029109844660368367
1201	12	-0.5489489312881304
1201	13	-0.10283426522727979
1201	14	0.34220670759804506
1201	15	-0.02597594519260582
1201	16	0.37446556157433264
1201	17	0.5152319059351171
1201	18	0.8104351157544514
1201	19	0.38577570015178964
1201	20	0.30508475525708434
1201	21	0.17132667655592115
1201	22	0.5733563561670394
1201	23	0.7759869882128356
1201	24	0.549515631295094
1201	25	0.45239410110259826
1202	1	0.7687890502493033
1202	2	0.8894890029697073
1202	3	0.5951716081750398
1202	4	1.0685183287335462
1202	5	1.0191553260887503
1202	6	1.0076066761922462
1202	7	0.9513110092462682
1202	8	1.0118252609498057
1202	9	1.2074007811413736
1202	10	1.3763622096822488
1202	11	0.9780542280959139
1202	12	1.2021103693614776
1202	13	1.3396336983719408
1202	14	1.3659982014996288
1202	15	0.984001617214787
1202	16	1.2853811021557888
1202	17	1.5785572924107925
1202	18	1.7218813159991129
1202	19	1.4160100533879287
1202	20	1.3192017406055285
1202	21	1.3243196677100784
1202	22	1.4758106129508022
1202	23	1.5034893399713027
1202	24	1.7269007988654579
1202	25	1.5701705185165125
1203	1	0.5331089466960997
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2211	15	0.5035524802690503
2211	16	0.32696431468222187
2211	17	0.639204336627941
2211	18	0.5715702914713603
2211	19	0.42499765348725305
2211	20	0.3121211855267134
2211	21	0.27833833831073207
2211	22	0.29642217280225935
2211	23	0.8298628870932987
2211	24	0.5650438902319905
2211	25	0.5783589900687645
2212	1	-0.16578365608515
2212	2	-0.22605675170183304
2212	3	-0.25505572456123105
2212	4	-0.32082990380683185
2212	5	-0.28229526371860914
2212	6	-0.3213400466839289
2212	7	-0.37018272784165873
2212	8	-0.3161459203776005
2212	9	-0.20616338315547722
2212	10	-0.08043408912493533
2212	11	-0.35370638968537094
2212	12	-0.08446848035360688
2212	13	-0.2977853056992643
2212	14	-0.2674007774878149
2212	15	-0.2893255224871396
2212	16	-0.12061121393528396
2212	17	-0.1637315918792126
2212	18	-2.8348180642214658
2212	19	-1.1583954784199193
2212	20	-0.5197529525198092
2212	21	-0.689874690406107
2212	22	-0.4022479199881346
2212	23	-0.40030257185375007
2212	24	-0.7100704529319728
2212	25	-0.3879294757745271
2213	1	-0.19298505546039735
2213	2	-0.06897092594568122
2213	3	-0.440030323721313
2213	4	-0.5281445639516136
2213	5	-0.4373662572636974
2213	6	-0.38243431211319034
2213	7	-0.20909873049232175
2213	8	-0.7442334125195842
2213	9	-0.5230473672190925
2213	10	-0.5609693943076621
2213	11	-0.5530990337706356
2213	12	-0.5743170555410657
2213	13	-0.4779396792937334
2213	14	-0.49063946761296506
2213	15	-0.6130840569425189
2213	16	-1.1016359237557012
2213	17	-0.7021552735144567
2213	18	-0.5428372118614675
2213	19	-0.496244876673984
2213	20	-1.1885552245400746
2213	21	-0.8624959995680683
2213	22	-0.7847481751711882
2213	23	-1.0469470564973946
2213	24	-0.9003170124163589
2213	25	-1.0140167378467098
2214	1	-1.5257423027530557
2214	2	-3.5316601737438873
2214	3	-1.8492666306952117
2214	4	-1.441184951227779
2214	5	-1.5178161115861286
2214	6	-1.4078389165886165
2214	7	-1.6458092279246728
2214	8	-1.239274804239244
2214	9	-1.551153908075328
2214	10	-1.57774268608852
2214	11	-1.2905713450549925
2214	12	-1.8331741185748882
2214	13	-1.772514354013687
2214	14	-2.8534302407018615
2214	15	-3.076680047816051
2214	16	-2.411203248077868
2214	17	-2.7999072491417323
2214	18	-2.1320347878931827
2214	19	-4.256508825799919
2214	20	-2.645930816563066
2214	21	-2.5421105360606333
2214	22	-2.635002528868334
2214	23	-2.863145952939938
2214	24	-2.9735262260235764
2214	25	-2.765478918220815
2215	1	-0.8598373063796991
2215	2	-2.4563750513699105
2215	3	-1.2354764624955372
2215	4	-0.9003678438441973
2215	5	-0.7622318436844395
2215	6	-1.304449682635017
2215	7	-1.6379252409797616
2215	8	-1.6097923629063011
2215	9	-1.3116816616594917
2215	10	-2.7254525530650113
2215	11	-2.0657507949326543
2215	12	-2.144975249396076
2215	13	-1.4809471303019066
2215	14	-2.7479325966821975
2215	15	-2.5209167723101444
2215	16	-2.203654401547574
2215	17	-2.441301965640342
2215	18	-1.3655372685329183
2215	19	-2.02178567046073
2215	20	-2.1868821487935293
2215	21	-2.0226462875942914
2215	22	-1.5517875465366444
2215	23	-2.1275753811153493
2215	24	-1.8041000130732314
2215	25	-1.7403776376026343
2216	1	-1.9583182014306502
2216	2	-1.9853061040847315
2216	3	-2.5395255243439365
2216	4	-3.3819094809353594
2216	5	-2.561236674074486
2216	6	-2.642822153690499
2216	7	-2.2792139587870324
2216	8	-2.9304416882027375
2216	9	-3.1577380019475605
2216	10	-2.6977138166528682
2216	11	-3.3100116505122443
2216	12	-3.7221897639464636
2216	13	-4.610030651275746
2216	14	-3.78563363053237
2216	15	-3.5715813329715758
2216	16	-2.7093732983665575
2216	17	-2.8426992029506337
2216	18	-2.823038699236079
2216	19	-3.103733634889135
2216	20	-3.0031900827206393
2216	21	-3.322679543324554
2216	22	-3.8587872187547205
2216	23	-3.90435474414873
2216	24	-3.8498532887913157
2216	25	-4.002347981361066
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	90173.0
3	3.0
4	731.0
5	4376.0
6	5368.0
7	4907.0
8	4235.0
9	3996.0
10	3736.0
11	3630.0
12	3462.0
13	3448.0
14	3522.0
15	3538.0
16	3558.0
17	3934.0
18	4230.0
19	5036.0
20	6787.0
21	8637.0
22	10754.0
23	13079.0
24	14932.0
25	17257.0
26	19219.0
27	21692.0
28	24237.0
29	29035.0
30	35597.0
31	44944.0
32	56675.0
33	72839.0
34	94734.0
35	125729.0
36	173895.0
37	276778.0
38	753502.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.21238110465669	18.825629417205246	12.429020103408938	25.532969374729124
2	14.947531976105102	20.46670585263037	41.0750551276815	23.510707043583025
3	18.431833939113808	24.97144470572802	29.804809382841817	26.791911972316353
4	12.235416860965039	17.08525795478886	38.65955133217796	32.01977385206814
5	13.032186253594286	37.920917759450965	36.21098300109513	12.835912985859613
6	31.273256936926597	37.58049468270148	18.49970053851833	12.646547841853598
7	27.546398407660444	31.671572281610477	22.86610873301927	17.915920577709805
8	24.791492675728712	36.753854272875856	21.070383710861645	17.384269340533784
9	26.437027772981608	15.143388962638893	20.288822203629593	38.130761060749904
10	14.709602913833589	28.803618006622628	35.11161275811523	21.375166321428555
11	34.02539075208537	22.335592600315707	25.224496404112486	18.41452024348644
12	22.924598435395374	24.7924098897276	32.20617039731981	20.076821277557215
13	28.85004275533955	22.006216815938902	25.843745500954757	23.299994927766786
14	20.802372541966797	22.02194847263512	27.990092546762707	29.18558643863538
15	22.89325900268492	30.733178250087107	23.538818634584246	22.834744112643726
16	22.252917990891348	27.999749970667665	27.717339790374997	22.029992248065987
17	21.804579079449887	28.382176779150566	27.858389360090914	21.954854781308633
18	21.610279879219522	27.538482367034806	29.55788355186504	21.293354201880632
19	23.390408186478908	27.03434841108241	27.53816404724892	22.037079355189768
20	23.453247878443513	27.374752209682484	27.924566197224955	21.24743371464905
21	23.08460877491511	26.839789575836576	27.224125713424918	22.851475935823398
22	23.143737210510515	27.956991450959578	27.223360204283438	21.675911134246476
23	22.37390329528896	27.661449484464246	27.648896685856222	22.31575053439057
24	22.87636583290254	26.959198824078516	28.23154258744738	21.932892755571565
25	22.69934438257398	27.324839067338612	27.840735586766325	22.135080963321087
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	304.0
1	304.0
2	451.5
3	599.0
4	599.0
5	599.0
6	1169.0
7	1739.0
8	1739.0
9	1739.0
10	2680.0
11	3621.0
12	3621.0
13	3621.0
14	6239.0
15	8857.0
16	8857.0
17	8857.0
18	16202.0
19	23547.0
20	23547.0
21	23547.0
22	37319.0
23	51091.0
24	51091.0
25	51091.0
26	72779.5
27	94468.0
28	94468.0
29	94468.0
30	120885.5
31	147303.0
32	147303.0
33	147303.0
34	167489.0
35	187675.0
36	187675.0
37	187675.0
38	203668.5
39	219662.0
40	219662.0
41	219662.0
42	231276.0
43	242890.0
44	242890.0
45	242890.0
46	246218.5
47	249547.0
48	249547.0
49	249547.0
50	241909.5
51	234272.0
52	234272.0
53	234272.0
54	210203.5
55	186135.0
56	186135.0
57	186135.0
58	160406.0
59	134677.0
60	134677.0
61	134677.0
62	109695.5
63	84714.0
64	84714.0
65	84714.0
66	65514.5
67	46315.0
68	46315.0
69	46315.0
70	33962.5
71	21610.0
72	21610.0
73	21610.0
74	15104.5
75	8599.0
76	8599.0
77	8599.0
78	5859.0
79	3119.0
80	3119.0
81	3119.0
82	2063.5
83	1008.0
84	1008.0
85	1008.0
86	661.5
87	315.0
88	315.0
89	315.0
90	205.5
91	96.0
92	96.0
93	96.0
94	60.0
95	24.0
96	24.0
97	24.0
98	21.0
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3643060026995116
2	0.0423111302347858
3	0.037291165630658665
4	0.03785463104540763
5	0.043233164549829554
6	0.018850479329783502
7	0.018440686300875163
8	0.024331461091432506
9	0.021309237503233523
10	0.021104340988779356
11	0.02007985841650851
12	0.020592099702643932
13	0.020592099702643932
14	0.027814701837153375
15	0.0289416326666513
16	0.02223127181827728
17	0.023511875033615834
18	0.11376878965067706
19	0.025560840178157518
20	0.023255754390548125
21	0.02704633990795024
22	0.020848220345711644
23	0.022999633747480413
24	0.019157824101464754
25	0.02340942677638875
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1952205.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.97698477813526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.79107326860597	60.41842893688539
2	11.823027616622369	17.25617812819447
3	3.0208689882107413	6.613617305081886
4	1.0505889624818554	3.0667525889246114
5	0.4393669674905983	1.6031838249288424
6	0.23950028426640985	1.0486805159561317
7	0.14191685548054475	0.7249664941515175
8	0.09615672630444476	0.5613782361467827
9	0.0663306274333442	0.4356548269674589
>10	0.2995166360140679	3.759760681594784
>50	0.018143649303688017	0.899237632163304
>100	0.011399400741915052	1.7930094272818127
>500	0.0013363441279403779	0.6818866421728347
>1k	7.736729161759955E-4	1.1372647595501173
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3010	0.1541846271267618	No Hit
GTCCTACAGTGGACATTTCTAAATT	2790	0.14291531883178252	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2759	0.1413273708447627	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2399	0.12288668454388754	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2309	0.11827651296866877	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1954	0.10009194731086131	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	5.122412861354212E-5	0.0
4	0.0	0.0	0.0	5.122412861354212E-5	0.0
5	0.0	5.122412861354212E-5	0.0	5.122412861354212E-5	0.0
6	0.0	5.122412861354212E-5	0.0	5.122412861354212E-5	0.0
7	0.0	5.122412861354212E-5	0.0	5.122412861354212E-5	0.0
8	0.0	5.122412861354212E-5	0.0	5.122412861354212E-5	0.0
9	0.0	5.122412861354212E-5	0.0	1.5367238584062636E-4	0.0
10	0.0	5.122412861354212E-5	0.0	1.5367238584062636E-4	0.0
11	0.0	5.122412861354212E-5	0.0	1.5367238584062636E-4	0.0
12	0.0	1.0244825722708424E-4	0.0	1.5367238584062636E-4	0.0
13	0.0	1.0244825722708424E-4	0.0	1.5367238584062636E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGC	55	1.9708797E-4	13.8096285	8
CTCGAAC	170	0.0	12.297536	18
GCGTGCG	125	1.4242687E-9	12.153408	9
TCGGGGC	55	0.003073149	12.08869	3
TAGGACC	645	0.0	12.075303	4
CGTGCGC	130	2.6393536E-9	11.685968	10
TCGAACT	190	0.0	11.493766	19
CGCCACC	170	1.0913936E-11	11.179578	14
GCCTCGA	190	0.0	11.003058	16
GGTATCA	975	0.0	10.944765	1
GTCCTAC	1440	0.0	10.917281	1
AGTCGTC	115	8.8896013E-7	10.732483	8
GTAGGAC	1530	0.0	10.677779	3
AGGACGT	1380	0.0	10.598961	5
GGACGTG	1360	0.0	10.540995	6
TGTAGGA	1600	0.0	10.507447	2
GACGTGG	685	0.0	10.394755	7
TAGGACT	320	0.0	10.388719	4
CTAGTAC	110	6.059865E-6	10.361734	3
GGCGTGC	165	9.385985E-10	10.357222	8
>>END_MODULE