FastQCFastQC Report
Thu 26 May 2016
SRR1512973_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512973_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1952205
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34850.1785160882181943No Hit
CTGTAGGACGTGGAATATGGCAAGA25950.1329266137521418No Hit
TATCAACGCAGAGTACTTTTTTTTT25870.13251682072323345No Hit
GTCCTACAGTGGACATTTCTAAATT25560.13092887273621368No Hit
GTCCTAAAGTGTGTATTTCTCATTT24460.12529421858872403No Hit
CTTTAGGACGTGAAATATGGCGAGG24300.12447463253090736No Hit
GGTATCAACGCAGAGTACTTTTTTT23420.11996690921291565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5650.012.6303744
TCGAACT1551.8189894E-1212.24550519
GGTATCA9000.012.0441421
CTCGAAC1604.7293724E-1111.269690518
AGGACCT9750.011.0275035
GTATTAG1252.2130916E-710.6495571
GGCGAGG6750.010.54474119
GGACCTG9800.010.3862976
GTCCTAC14250.010.2758881
TGGCGAG14850.010.225203518
GCTCTAT1409.5637006E-810.1877151
CTACCGT750.00261392310.1492074
TAGAACA6100.010.1388084
CCCTTAG1606.0827006E-910.1028161
GTAGGAC16500.010.0837733
TCTATAC1909.822543E-1110.0079563
GGACGTG15500.09.9422886
AGGACGT15800.09.9364275
ATAGGAC2400.09.9037063
TGTAGGA16900.09.7878412
Produced by FastQC (version 0.11.5)