FastQCFastQC Report
Thu 26 May 2016
SRR1512972_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512972_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145220
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30020.13993902723263815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGG5200.011.13733819
GGTATCA10650.010.4643211
TAGGACC3750.010.3836324
CTTATAC2700.010.2007233
GTATTAG1502.347042E-810.160231
CTAAGGT1701.5679689E-910.0558854
GTAGGAC10600.010.0348043
GTCCTAC11750.09.890011
GTAAGAC1554.0830855E-89.8035873
TGTAGAA7900.09.7376612
AGGACGT11050.09.7114145
TGGCGAG10500.09.68444818
GTATTAA2500.09.5252151
TGTAGGA12400.09.4972252
AGGACCG800.0045301419.496565
CCGCTTG800.0045371179.4947899
GCCGTCC800.00453886259.4943478
TCCTACA14100.09.3625132
GTAGAAC6900.09.3595843
TAGGACG11600.09.3334794
Produced by FastQC (version 0.11.5)