##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512972_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2145220
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.108631748725074	31.0	30.0	33.0	25.0	34.0
2	29.253486355711768	31.0	30.0	34.0	23.0	34.0
3	29.537917789317646	31.0	30.0	34.0	25.0	34.0
4	33.00374180736708	35.0	35.0	37.0	26.0	37.0
5	33.19122374395167	37.0	35.0	37.0	27.0	37.0
6	33.19938328003655	37.0	35.0	37.0	27.0	37.0
7	33.010785373994274	37.0	35.0	37.0	26.0	37.0
8	33.070213311455234	37.0	35.0	37.0	26.0	37.0
9	34.42134233318727	39.0	35.0	39.0	27.0	39.0
10	34.28335974865049	39.0	35.0	39.0	26.0	39.0
11	34.38130401543898	39.0	35.0	39.0	27.0	39.0
12	34.27220005407371	39.0	35.0	39.0	26.0	39.0
13	34.281073736027075	39.0	35.0	39.0	26.0	39.0
14	35.31480407603882	40.0	36.0	41.0	25.0	41.0
15	35.244344169828736	40.0	35.0	41.0	25.0	41.0
16	35.1439017909585	40.0	35.0	41.0	25.0	41.0
17	35.10013098889625	39.0	35.0	41.0	25.0	41.0
18	34.77627888981083	39.0	34.0	41.0	20.0	41.0
19	35.02071815478133	39.0	35.0	41.0	24.0	41.0
20	35.08825714845098	40.0	35.0	41.0	25.0	41.0
21	35.047468324927046	40.0	35.0	41.0	24.0	41.0
22	34.96104641948145	40.0	35.0	41.0	23.0	41.0
23	34.69819505691724	39.0	35.0	41.0	19.0	41.0
24	34.70216061755904	39.0	35.0	41.0	19.0	41.0
25	34.62882361715815	39.0	35.0	41.0	19.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5271890042671181
1101	2	0.8493910375165576
1101	3	0.9253688234520077
1101	4	0.970046787094411
1101	5	0.7972628314026196
1101	6	0.9256384177980124
1101	7	0.9126890776496523
1101	8	0.7539186097631045
1101	9	0.6984607908750178
1101	10	0.8540154763323642
1101	11	1.01573465667866
1101	12	1.0899804134236888
1101	13	0.9318374270308851
1101	14	0.9857099674235883
1101	15	0.8674817831718258
1101	16	1.2411285693436511
1101	17	1.2218476636899922
1101	18	1.4124711201278117
1101	19	0.8138581136405136
1101	20	1.075341386475415
1101	21	0.9746609188204616
1101	22	1.123982695492323
1101	23	1.412935781154907
1101	24	0.9891624384423139
1101	25	1.2160566120450937
1102	1	0.2653447542778409
1102	2	0.46507756434708014
1102	3	0.4346914883048498
1102	4	0.6437259421948767
1102	5	0.4440157160592477
1102	6	0.42051099042762985
1102	7	0.4994294616169057
1102	8	0.5219756717334363
1102	9	0.5020207298422434
1102	10	0.7227635996970108
1102	11	0.6327562755179343
1102	12	0.6099983678451935
1102	13	0.6783782901912332
1102	14	0.688899021967039
1102	15	0.8553257819217563
1102	16	0.7741481598847955
1102	17	0.7471385804684871
1102	18	0.9640012290186206
1102	19	1.0356649228395511
1102	20	0.9575277509195317
1102	21	0.6609587232336978
1102	22	0.9499848953685444
1102	23	1.112828085264816
1102	24	1.0052777573018048
1102	25	0.7923100512666181
1103	1	0.2863567406813239
1103	2	0.5407260824459534
1103	3	0.5167267915429683
1103	4	0.7073656941180673
1103	5	0.3418338626392412
1103	6	0.6058656627396317
1103	7	0.5655537087395714
1103	8	0.5394036638617692
1103	9	0.8890622946344209
1103	10	0.8544112829220083
1103	11	0.8452115501318076
1103	12	1.000483041946211
1103	13	1.0656636083378146
1103	14	1.002544737275045
1103	15	1.1611549905805916
1103	16	1.1952883590618484
1103	17	1.0958803062720577
1103	18	1.3267598908350706
1103	19	1.3336224635396476
1103	20	1.252190521508119
1103	21	0.9826557534680589
1103	22	1.0934482801857612
1103	23	1.452250531445273
1103	24	1.2699253212591088
1103	25	1.1951881298640359
1104	1	0.46359251303904614
1104	2	0.5068215663934943
1104	3	0.5925843074173969
1104	4	0.7709998998031935
1104	5	0.5765239403794382
1104	6	0.496218609152308
1104	7	0.6909668889662655
1104	8	0.7167225173422693
1104	9	0.8989163092329591
1104	10	0.8525108022383705
1104	11	0.9018276263290659
1104	12	0.7923522188496648
1104	13	0.7272200441576473
1104	14	1.0132651692968864
1104	15	1.0132453709734506
1104	16	0.8686371217465592
1104	17	0.850467571122806
1104	18	0.9301498973628739
1104	19	0.8132201135307469
1104	20	0.8927430109746197
1104	21	0.9545513382998152
1104	22	0.8587804563177457
1104	23	1.134911797838953
1104	24	0.9986135296740386
1104	25	0.8120424708526954
1105	1	0.4871454072506225
1105	2	0.6432454339867739
1105	3	0.6717107261128525
1105	4	0.8414134522603192
1105	5	0.5859827599665408
1105	6	0.42924999164280564
1105	7	0.7413588058624043
1105	8	0.7970931629124465
1105	9	0.7209485221793273
1105	10	0.8168765961978934
1105	11	0.8308859330732403
1105	12	0.8033255277063986
1105	13	0.8961970536423252
1105	14	0.9727141545380817
1105	15	1.1375169009062844
1105	16	1.0645340140932547
1105	17	0.9652281958587352
1105	18	1.1642142504086053
1105	19	1.0123284661685403
1105	20	1.0622277640961144
1105	21	1.035333933692975
1105	22	0.8588948261182878
1105	23	0.8924980851184117
1105	24	1.0844316744064741
1105	25	1.208699521714479
1106	1	0.7023487319445216
1106	2	0.8029102833924426
1106	3	0.7134195472764944
1106	4	0.7764719918974663
1106	5	0.7499035750527838
1106	6	0.7040609667076012
1106	7	0.5919379460894092
1106	8	0.7613330413644945
1106	9	0.8798831901568036
1106	10	0.9559256622449084
1106	11	0.8087472254139314
1106	12	0.9430431074722421
1106	13	0.9290900735726169
1106	14	1.1302840892954293
1106	15	1.2190326753275897
1106	16	1.195734300088425
1106	17	1.0902760106768596
1106	18	1.2568436185380207
1106	19	0.7718386994333528
1106	20	1.0327711549263938
1106	21	0.9553232088739563
1106	22	0.9235547599819043
1106	23	1.0578220640262685
1106	24	1.0598834574076363
1106	25	1.1320983956084802
1107	1	0.4027045429661982
1107	2	0.43327358795644955
1107	3	0.5758846582250001
1107	4	0.7698399424577147
1107	5	0.6263902497713403
1107	6	0.48053089115506253
1107	7	0.772139830935707
1107	8	0.6238907196006025
1107	9	0.7716819047939012
1107	10	0.6646456770556739
1107	11	0.6330248920130046
1107	12	0.7668954935042791
1107	13	0.7629074603540076
1107	14	0.9935507447952503
1107	15	1.0261637065271714
1107	16	0.9397839866820377
1107	17	1.005389692121966
1107	18	1.291922036254519
1107	19	1.1818655880264188
1107	20	0.9497864329368113
1107	21	0.9934658682880482
1107	22	0.9216265393014922
1107	23	1.0787480895395731
1107	24	1.0348486785552922
1107	25	1.0726619099916732
1108	1	0.40898475567538384
1108	2	0.4950411426569232
1108	3	0.40771203932195377
1108	4	0.6570653505634496
1108	5	0.517741028206963
1108	6	0.6751494438320407
1108	7	0.7106551213678713
1108	8	0.7249765038421359
1108	9	0.9140185501024831
1108	10	0.8254644947664644
1108	11	0.7893969949390112
1108	12	0.8023280476009944
1108	13	0.7961402992595765
1108	14	1.0220135435116617
1108	15	1.0004777683228028
1108	16	0.9268660429489302
1108	17	1.007018979994747
1108	18	1.1880534486525391
1108	19	1.1754365457224694
1108	20	0.9123447654390091
1108	21	0.8360864094937739
1108	22	0.7997918596976774
1108	23	0.9989823170045966
1108	24	0.998020157981756
1108	25	1.0626911884248003
1109	1	-0.19093631124652433
1109	2	-0.2906853102796596
1109	3	0.027968841741575545
1109	4	0.20168723871292826
1109	5	-0.048660472109801844
1109	6	-0.025151676690654767
1109	7	0.044403709824869964
1109	8	0.061326665648891776
1109	9	0.10010501192898857
1109	10	0.405225617526348
1109	11	-0.19453989873984767
1109	12	0.08545755555222456
1109	13	0.14460821115524425
1109	14	-0.014451739126975838
1109	15	0.24337312083071794
1109	16	0.276137386997398
1109	17	0.02296226791042244
1109	18	-1.3721381708242788
1109	19	-0.11759921924970484
1109	20	0.11051999600725537
1109	21	0.23580519526357335
1109	22	0.32344345061358837
1109	23	-0.05769784458847482
1109	24	0.3536297659125722
1109	25	0.3805468386498774
1110	1	0.2265478997524717
1110	2	0.19122714444079492
1110	3	0.25509509705536004
1110	4	-0.02245034556774783
1110	5	0.20828106225150833
1110	6	0.21407099837276888
1110	7	0.31792484386237874
1110	8	0.17461200016488476
1110	9	0.40655206669753596
1110	10	0.5375889995618266
1110	11	0.3818063598993078
1110	12	-0.08701873992432496
1110	13	0.24399695355028683
1110	14	0.2600449867369221
1110	15	0.39638257108550334
1110	16	0.5621003075729192
1110	17	0.19990483906560286
1110	18	0.5863442160763341
1110	19	0.46140681443985443
1110	20	0.41384288569394556
1110	21	0.46826946531820823
1110	22	0.47964537644953964
1110	23	0.5492630473039171
1110	24	0.7148993772240999
1110	25	0.6918576356444177
1111	1	0.07525616584194239
1111	2	0.4286794484172276
1111	3	0.3055280823587694
1111	4	0.2500386972495505
1111	5	0.21958842386478494
1111	6	0.3632593689099721
1111	7	0.19965649619602033
1111	8	0.06452899648941468
1111	9	0.45978260807625304
1111	10	0.33243065986080467
1111	11	0.21056112157507556
1111	12	0.37086062026991584
1111	13	0.2307294355331564
1111	14	0.26322337118615735
1111	15	0.4550662506696881
1111	16	0.2867925711202446
1111	17	0.3206825674049725
1111	18	0.5624425677341733
1111	19	0.39250013007399787
1111	20	0.25894120881607563
1111	21	0.36814859370387154
1111	22	0.1992975649444375
1111	23	0.5225650759107836
1111	24	0.6637325662935822
1111	25	0.15744885848397416
1112	1	-0.30296249230127614
1112	2	-0.032210906463696176
1112	3	-0.014034009292540617
1112	4	0.10021007398918869
1112	5	-0.06975478911240884
1112	6	0.02395897158562832
1112	7	-0.2298928359166652
1112	8	-0.011472694013377804
1112	9	0.35258545222253446
1112	10	0.32750102168423467
1112	11	0.15010194323177473
1112	12	0.18905264614647876
1112	13	-6.331135793047338E-4
1112	14	0.11625954264331284
1112	15	0.09426725106527556
1112	16	0.2719167435367851
1112	17	0.23463241102292898
1112	18	0.17174934043827506
1112	19	0.2197392781013292
1112	20	0.1319566721407881
1112	21	0.09894181841871585
1112	22	0.21368079743228918
1112	23	0.34652330005410903
1112	24	0.06503181987725526
1112	25	0.36072163536658053
1113	1	-0.22216436328332279
1113	2	-0.1850715615662608
1113	3	-0.3957072365768859
1113	4	-0.07023658868490656
1113	5	-0.2686342034522653
1113	6	-0.11703643147173182
1113	7	-0.31324433638167193
1113	8	0.015259714978540728
1113	9	-0.10933643589068254
1113	10	-0.3983394805925542
1113	11	-0.3910629562226049
1113	12	-0.051527605035616375
1113	13	-0.2800249973508997
1113	14	-0.20315804796977233
1113	15	-0.591398033218546
1113	16	-0.4537106276100715
1113	17	-0.5228652076400948
1113	18	-0.6052936466651033
1113	19	-0.4905777813479659
1113	20	-0.646994241978291
1113	21	-0.44073435673137595
1113	22	-0.375295650401263
1113	23	-0.8162944279512345
1113	24	-0.18036354761342466
1113	25	-0.3125161542618997
1114	1	-1.2954623822637252
1114	2	-1.1023633484483533
1114	3	-0.9890543312088376
1114	4	-1.1984692610905654
1114	5	-1.1176353010252242
1114	6	-1.4215804357875648
1114	7	-1.6869551943205963
1114	8	-1.146497753726262
1114	9	-1.2522931100062138
1114	10	-1.5557892774876692
1114	11	-2.038099525551111
1114	12	-1.6732886463224546
1114	13	-1.4722473817652997
1114	14	-1.7946683978444185
1114	15	-1.6437762004970224
1114	16	-2.2631664423792017
1114	17	-4.468598209056392
1114	18	-3.529339050459946
1114	19	-2.4654389192643293
1114	20	-1.4849100485637976
1114	21	-2.368485253060854
1114	22	-2.4919118796552198
1114	23	-2.5377776960865788
1114	24	-2.022752429372389
1114	25	-1.823588872634346
1115	1	-1.014045155800094
1115	2	-0.7161821828679251
1115	3	-0.8369336456706442
1115	4	-0.9351999121132266
1115	5	-0.6411789268609951
1115	6	-0.818311106054125
1115	7	-1.771500853113782
1115	8	-1.2446331323010362
1115	9	-0.9950195982530872
1115	10	-0.8009778620717398
1115	11	-1.4660571883348368
1115	12	-1.1977743411305894
1115	13	-1.0449133014480054
1115	14	-1.1379006317578444
1115	15	-2.3884476053320185
1115	16	-1.2610125691358718
1115	17	-1.173223944914973
1115	18	-1.2506827620272745
1115	19	-1.3110236394813128
1115	20	-1.1598592887746264
1115	21	-1.8574342844753957
1115	22	-1.8187918520979593
1115	23	-1.1915235287804578
1115	24	-1.257781518929157
1115	25	-1.56681770679252
1116	1	-1.0524511006681188
1116	2	-1.7325814169841784
1116	3	-1.4049663603017706
1116	4	-1.1149103487358154
1116	5	-1.7594226908141408
1116	6	-1.4355086086466038
1116	7	-1.0733742114141265
1116	8	-1.8187291310843392
1116	9	-1.8783943141773278
1116	10	-2.874716985663749
1116	11	-1.4683581688430891
1116	12	-1.463781912953003
1116	13	-2.013079186933929
1116	14	-1.7776727885317243
1116	15	-1.401828173964006
1116	16	-1.3126091628372691
1116	17	-1.6404160563577932
1116	18	-1.3827845423929759
1116	19	-1.8386592779693558
1116	20	-1.9842409806573684
1116	21	-2.5830786403578614
1116	22	-1.6905747260990367
1116	23	-1.6198301289474486
1116	24	-2.3782063339559727
1116	25	-2.029179726464733
1201	1	0.3611143169105624
1201	2	0.3678557538703018
1201	3	0.5568014253780085
1201	4	0.765539227512626
1201	5	0.6737378815039392
1201	6	0.6610606523889544
1201	7	0.5325947768050057
1201	8	0.08816080427299511
1201	9	0.3317712927199352
1201	10	0.6510897270516978
1201	11	0.29087068596694365
1201	12	-0.11674791825426212
1201	13	0.11165739014863618
1201	14	0.668214729648227
1201	15	0.4546184986043116
1201	16	0.7875934472371782
1201	17	0.9318683679714326
1201	18	1.231108732026101
1201	19	0.8148807324082341
1201	20	0.7663121925589067
1201	21	0.6602031486771693
1201	22	0.8403300412018453
1201	23	1.0105555884907318
1201	24	0.9059608559922481
1201	25	0.8361544395749902
1202	1	0.35907005889610133
1202	2	0.43084348267118955
1202	3	0.34928915559165574
1202	4	0.7143607265921119
1202	5	0.6979377872932915
1202	6	0.7273592759205485
1202	7	0.7279387768502161
1202	8	0.7602734437170611
1202	9	1.0583696950139228
1202	10	1.0955306732430472
1202	11	0.8272588419045306
1202	12	0.9177596575491833
1202	13	0.905744562041086
1202	14	1.132709344423965
1202	15	0.8237327045930272
1202	16	0.9352256479034295
1202	17	1.168706627988783
1202	18	1.5299671847675924
1202	19	1.2597683113358897
1202	20	1.0153895619425484
1202	21	1.101570341333101
1202	22	1.128448318753513
1202	23	1.3136654278522997
1202	24	1.4682426104092343
1202	25	1.2353678170024622
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2211	12	0.19376259615324187
2211	13	0.09604054847085308
2211	14	0.39458727432720764
2211	15	0.3993085090774997
2211	16	0.3806250377874889
2211	17	0.40229879150953707
2211	18	0.6053396551016093
2211	19	0.3752901027040778
2211	20	0.07907230468001814
2211	21	0.24214781788630546
2211	22	0.009506163252630984
2211	23	0.6306960910204822
2211	24	0.5541402238148336
2211	25	0.5680160561098049
2212	1	-0.19073117962820518
2212	2	-0.2775021156116608
2212	3	-0.15151049139953798
2212	4	-0.40456881633132724
2212	5	-0.3401877736375951
2212	6	-0.2987607703564237
2212	7	-0.37630026687730833
2212	8	-0.3688461241575851
2212	9	-0.326968964495002
2212	10	-0.18979612321866313
2212	11	-0.42385165572812156
2212	12	-0.2670375148325377
2212	13	-0.4257455641894978
2212	14	-0.458506401720129
2212	15	-0.5561885890778129
2212	16	-0.37939705453198513
2212	17	-0.3774669572181111
2212	18	-3.005064282796674
2212	19	-1.3544018768264507
2212	20	-0.7665601464981222
2212	21	-0.8786591143914038
2212	22	-0.4670270374289345
2212	23	-0.5422570321667379
2212	24	-0.9559899363597211
2212	25	-0.6605655414982721
2213	1	-0.360410817355703
2213	2	-0.2931264006571901
2213	3	-0.6395838167438406
2213	4	-0.7503848201340588
2213	5	-0.6833534720654271
2213	6	-0.6266460915947789
2213	7	-0.5353462324887559
2213	8	-0.967355078424454
2213	9	-0.8352912169729905
2213	10	-0.8941047323586275
2213	11	-0.9083551557159737
2213	12	-0.9991900406279868
2213	13	-0.8835246079419363
2213	14	-0.8785307843235444
2213	15	-1.1145282301070338
2213	16	-1.4916275974471134
2213	17	-1.290095303484044
2213	18	-1.1267223307341325
2213	19	-0.8751345155964074
2213	20	-1.7354747738195329
2213	21	-1.3165691951067302
2213	22	-1.2142324427058
2213	23	-1.5215005705235782
2213	24	-1.2088066799163215
2213	25	-1.4172971772179537
2214	1	-1.35535559921615
2214	2	-3.3450445517594787
2214	3	-1.6509280665622477
2214	4	-1.319245302912556
2214	5	-1.188733162519199
2214	6	-1.4640703331830132
2214	7	-1.478287606944935
2214	8	-1.177641438145887
2214	9	-1.4020188682971408
2214	10	-1.4237335221480905
2214	11	-1.0585066324921755
2214	12	-1.641981798283652
2214	13	-1.5347926930907363
2214	14	-2.705985200235112
2214	15	-2.8840142342794337
2214	16	-2.160959517317579
2214	17	-2.5247350635703185
2214	18	-1.781325378994005
2214	19	-4.16076822355317
2214	20	-2.5457726493713224
2214	21	-2.3178046105389782
2214	22	-2.284388322907702
2214	23	-2.4132482054621462
2214	24	-2.694096853032896
2214	25	-2.490351860681237
2215	1	-0.7084387521323272
2215	2	-2.4242878071336627
2215	3	-1.1692918013111822
2215	4	-0.7585140739863405
2215	5	-0.8300227762956283
2215	6	-1.205869167068613
2215	7	-1.5042884083023367
2215	8	-1.3860721696906282
2215	9	-1.2081485996271581
2215	10	-2.9830145623530484
2215	11	-1.7153218843242968
2215	12	-1.974069987118888
2215	13	-1.4043616309110831
2215	14	-2.7596507399083876
2215	15	-2.3618935412446618
2215	16	-1.9742236584439894
2215	17	-2.0875347896761625
2215	18	-1.1469223752622781
2215	19	-1.6491800344532876
2215	20	-1.925427539918637
2215	21	-1.6237497050301641
2215	22	-1.179269974487319
2215	23	-1.6610502507760714
2215	24	-1.549590959033317
2215	25	-1.5396570421749303
2216	1	-1.7476281442379467
2216	2	-1.7566356489178396
2216	3	-2.3183693953343933
2216	4	-3.0449616847895236
2216	5	-2.2289948903665966
2216	6	-2.2197775870027243
2216	7	-2.080544711047441
2216	8	-2.6076179781856403
2216	9	-3.0007264377599476
2216	10	-2.36055951749022
2216	11	-2.9458628229358688
2216	12	-3.3850880676381223
2216	13	-3.9925285936546295
2216	14	-3.345301363721177
2216	15	-3.2428676820404974
2216	16	-2.4137927127347822
2216	17	-2.415584825984901
2216	18	-2.492553060040919
2216	19	-2.827236347865366
2216	20	-2.7829194063517235
2216	21	-3.0054419792698326
2216	22	-3.4966470478801916
2216	23	-3.480025318778022
2216	24	-3.5302069356972616
2216	25	-3.617466456188872
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	90022.0
3	6.0
4	678.0
5	4297.0
6	5507.0
7	4976.0
8	4398.0
9	4196.0
10	3826.0
11	3670.0
12	3620.0
13	3604.0
14	3604.0
15	3633.0
16	3918.0
17	3996.0
18	4716.0
19	5640.0
20	7101.0
21	9196.0
22	11449.0
23	13980.0
24	16240.0
25	18655.0
26	21290.0
27	23244.0
28	26715.0
29	31929.0
30	39321.0
31	49515.0
32	62886.0
33	80854.0
34	106058.0
35	140225.0
36	194228.0
37	309614.0
38	828413.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.58336202637764	18.43216215811614	12.41831838799982	25.566157427506397
2	15.228237730050619	20.187048380289735	40.51217959629074	24.0725342933689
3	18.68329003553823	24.432547071488226	29.72994020380974	27.1542226891638
4	12.516694086003454	16.768743075218513	38.096510469710026	32.618052369068
5	13.408893032070745	37.58998635019514	35.7746602355116	13.226460382222516
6	31.971744042162943	36.93224892322067	18.144302883875678	12.951704150740714
7	27.942850135932346	31.58866234275465	22.329177734044443	18.139309787268566
8	25.273723053164893	36.13894994418763	20.765184274061564	17.82214272858591
9	26.58093460105262	15.146516320828965	19.764083732137728	38.50846534598069
10	14.967287776184232	28.727123357161904	34.58898041929089	21.716608447362972
11	34.50166101339301	22.31352939119488	24.458975883251156	18.72583371216095
12	22.97540567419337	24.58108425033442	31.70424890768817	20.73926116778404
13	28.851765906069808	21.69419185243311	25.75533986738861	23.698702374108468
14	21.453787362697057	21.41517910640538	27.588303563476774	29.54272996742079
15	23.416432433642264	29.76231332078702	23.40976448623232	23.41148975933839
16	22.8613724740528	27.774508798269252	26.751074722343965	22.61304400533398
17	22.150085909250922	27.89152820551078	27.484525843880625	22.473860041357668
18	22.099983806287735	27.493143339554145	28.545034620476784	21.861838233681336
19	23.596925393942644	26.93241135561964	27.173101626594367	22.29756162384335
20	23.688941856225387	27.13573532744165	27.419781994649178	21.755540821683784
21	23.564305837524397	26.61838199568972	26.863551119487543	22.953761047298336
22	23.469918233694877	27.23722019740103	26.85382262255504	22.43903894634905
23	23.100139412787744	27.11359577751771	26.917671853071045	22.868592956623505
24	23.307915208679617	26.708286805965137	27.455164712711472	22.528633272643773
25	23.266315725669205	26.772057760722895	27.257018431396318	22.704608082211582
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	266.0
1	266.0
2	443.0
3	620.0
4	620.0
5	620.0
6	1156.0
7	1692.0
8	1692.0
9	1692.0
10	2588.5
11	3485.0
12	3485.0
13	3485.0
14	6244.0
15	9003.0
16	9003.0
17	9003.0
18	16365.5
19	23728.0
20	23728.0
21	23728.0
22	36935.0
23	50142.0
24	50142.0
25	50142.0
26	71579.5
27	93017.0
28	93017.0
29	93017.0
30	120570.5
31	148124.0
32	148124.0
33	148124.0
34	169784.0
35	191444.0
36	191444.0
37	191444.0
38	210373.0
39	229302.0
40	229302.0
41	229302.0
42	245111.5
43	260921.0
44	260921.0
45	260921.0
46	269422.0
47	277923.0
48	277923.0
49	277923.0
50	272930.5
51	267938.0
52	267938.0
53	267938.0
54	244705.0
55	221472.0
56	221472.0
57	221472.0
58	191607.5
59	161743.0
60	161743.0
61	161743.0
62	132686.5
63	103630.0
64	103630.0
65	103630.0
66	80534.0
67	57438.0
68	57438.0
69	57438.0
70	42076.0
71	26714.0
72	26714.0
73	26714.0
74	18779.5
75	10845.0
76	10845.0
77	10845.0
78	7427.5
79	4010.0
80	4010.0
81	4010.0
82	2627.5
83	1245.0
84	1245.0
85	1245.0
86	811.5
87	378.0
88	378.0
89	378.0
90	240.0
91	102.0
92	102.0
93	102.0
94	62.5
95	23.0
96	23.0
97	23.0
98	19.0
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35670001211996905
2	0.039856052059928586
3	0.03584713922115214
4	0.03486821864424162
5	0.0404154352467346
6	0.01855287569573284
7	0.018785952023568676
8	0.02489255181286768
9	0.022188866409971936
10	0.02125656109862858
11	0.02074379317738973
12	0.021303176364195747
13	0.021303176364195747
14	0.028015774605867932
15	0.029460847838450138
16	0.02237532747224061
17	0.02400686176709149
18	0.11248263581357623
19	0.025871472389778206
20	0.023400863314718304
21	0.026943623497823067
22	0.020930254239658403
23	0.023773785439255648
24	0.019811487866046373
25	0.023773785439255648
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2145220.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.08364186279738
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.20441680365211	56.210176230816835
2	12.979626727410704	18.19319022153289
3	3.7007391469161197	7.780838310003109
4	1.364120188614845	3.8241004302677766
5	0.614717398018146	2.1540816984767206
6	0.32573590821491755	1.3697255239912394
7	0.19571310733502081	0.9601401125625977
8	0.13532341639808212	0.7587166280394714
9	0.09118218199223649	0.5751341448311084
>10	0.3562472240226916	4.156365772646863
>50	0.018886327678701492	0.9076765486967455
>100	0.010956281854462322	1.505278009543911
>500	0.0017347852912317227	0.8533985757062413
>1k	6.005026008107338E-4	0.7511777928844344
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3002	0.13993902723263815	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGAGG	520	0.0	11.137338	19
GGTATCA	1065	0.0	10.464321	1
TAGGACC	375	0.0	10.383632	4
CTTATAC	270	0.0	10.200723	3
GTATTAG	150	2.347042E-8	10.16023	1
CTAAGGT	170	1.5679689E-9	10.055885	4
GTAGGAC	1060	0.0	10.034804	3
GTCCTAC	1175	0.0	9.89001	1
GTAAGAC	155	4.0830855E-8	9.803587	3
TGTAGAA	790	0.0	9.737661	2
AGGACGT	1105	0.0	9.711414	5
TGGCGAG	1050	0.0	9.684448	18
GTATTAA	250	0.0	9.525215	1
TGTAGGA	1240	0.0	9.497225	2
AGGACCG	80	0.004530141	9.49656	5
CCGCTTG	80	0.004537117	9.494789	9
GCCGTCC	80	0.0045388625	9.494347	8
TCCTACA	1410	0.0	9.362513	2
GTAGAAC	690	0.0	9.359584	3
TAGGACG	1160	0.0	9.333479	4
>>END_MODULE