FastQCFastQC Report
Thu 26 May 2016
SRR1512972_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512972_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145220
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36910.17205694520841686No Hit
TATCAACGCAGAGTACTTTTTTTTT26130.12180568892700983No Hit
GGTATCAACGCAGAGTACTTTTTTT23280.10852033824036696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2100.012.65375318
TAGGACC3650.012.251824
CCAACGA1405.2750693E-1112.20183419
GGTATCA10700.011.0171251
AGGACGT10700.010.9374715
GGACGTG10600.010.8578346
ACGAAAT803.801913E-410.67834712
GTCCTAC11750.010.5989741
CGTCTAT909.450089E-510.5630151
GGACCGT1002.3668292E-510.4630036
GACGTGA6650.010.291067
AGGATAG1951.4551915E-1110.2466135
CCGGTTT1951.4551915E-1110.22200712
TGTAGGA12400.010.1202862
GTCCTAG2650.010.0448281
TAGGACG12050.010.0279534
CTACTAG951.6328019E-410.0070671
TGGACCG1151.0080103E-59.9283945
TGGCGAG12250.09.9164118
AGGACCG1252.5203499E-69.89535
Produced by FastQC (version 0.11.5)