FastQCFastQC Report
Thu 26 May 2016
SRR1512971_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512971_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1858875
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35810.19264339990585705No Hit
GGTATCAACGCAGAGTACTTTTTTT21000.11297155537623561No Hit
TATCAACGCAGAGTACTTTTTTTTT20310.10925963284244503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3350.012.7580314
TAAGGCG752.0790125E-411.3971744
GTAGGAC8400.010.4024623
AATCCCG1106.087439E-610.35772119
GACTGAT1201.5355581E-610.2855167
GGTATCA9650.010.1702331
AGCACGG856.610917E-410.0560595
GTATCAA18400.09.9944861
AGGACGT7350.09.9496455
TGTAGGA9850.09.9318142
GGACGTG7350.09.8172586
GACGTGA4450.09.8143567
GTCCTAC8750.09.8006631
TAGGACG7700.09.7443374
GTCCTAG2750.09.7016661
CTGTAGG9650.09.6765331
GTCCTAA5800.09.5284221
GTATAAG2600.09.5284221
ATTGGAC1401.061675E-69.4979013
CGTGAAA5050.09.4010789
Produced by FastQC (version 0.11.5)