##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512971_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1858875
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.036601708022324	31.0	30.0	34.0	23.0	34.0
2	29.06235653284917	31.0	30.0	34.0	22.0	34.0
3	29.20103987626925	31.0	30.0	34.0	22.0	34.0
4	32.62199878959048	35.0	33.0	37.0	25.0	37.0
5	32.7232939277789	35.0	33.0	37.0	25.0	37.0
6	32.7340923945935	37.0	35.0	37.0	25.0	37.0
7	32.553641853271465	36.0	33.0	37.0	24.0	37.0
8	32.56107699549459	36.0	33.0	37.0	25.0	37.0
9	33.87301728195818	38.0	34.0	39.0	23.0	39.0
10	33.72777351892946	38.0	34.0	39.0	22.0	39.0
11	33.83196072893551	38.0	34.0	39.0	23.0	39.0
12	33.72646842848497	38.0	34.0	39.0	22.0	39.0
13	33.73894048819851	38.0	34.0	39.0	22.0	39.0
14	34.75288010221236	39.0	34.0	41.0	20.0	41.0
15	34.692276242350886	39.0	34.0	41.0	19.0	41.0
16	34.58794970075987	39.0	34.0	41.0	18.0	41.0
17	34.53711572859929	39.0	34.0	41.0	18.0	41.0
18	34.23756089032345	39.0	34.0	41.0	17.0	41.0
19	34.47751785354045	39.0	34.0	41.0	18.0	41.0
20	34.55272247999462	39.0	34.0	41.0	18.0	41.0
21	34.494027839419005	39.0	34.0	41.0	18.0	41.0
22	34.40463990316724	39.0	34.0	41.0	17.0	41.0
23	34.14314356801829	39.0	34.0	41.0	10.0	41.0
24	34.14488117813193	39.0	34.0	41.0	2.0	41.0
25	34.07062605070271	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6927239376529606
1101	2	0.8469489825890584
1101	3	0.8665523779217423
1101	4	0.8779811822868879
1101	5	0.6011813250980893
1101	6	0.7698098180105077
1101	7	0.7614085143381573
1101	8	0.6040507210071411
1101	9	0.5341128661194432
1101	10	0.6338896377315493
1101	11	0.8324021525698271
1101	12	0.8947110314702584
1101	13	0.9026837870779261
1101	14	0.7411940409730207
1101	15	0.5840837609821747
1101	16	1.0201495706781358
1101	17	1.132518341925703
1101	18	1.3520487684801452
1101	19	0.7789883193757419
1101	20	1.020178379501175
1101	21	0.867719196581568
1101	22	0.8085179670946445
1101	23	1.1578986516394778
1101	24	0.7865360097355847
1101	25	1.0019460136771272
1102	1	0.9099997281938066
1102	2	0.950321504355049
1102	3	0.8601005159263018
1102	4	1.015561911516805
1102	5	0.936647385972492
1102	6	0.7715970546499733
1102	7	0.9760398173831248
1102	8	0.9660284135538646
1102	9	0.9613548361648583
1102	10	1.1873095181548337
1102	11	1.0917639409522906
1102	12	1.1295646368621135
1102	13	1.0140651217264676
1102	14	1.191132670878659
1102	15	1.260166113359233
1102	16	1.240747254992364
1102	17	1.146564029174236
1102	18	1.341529230620928
1102	19	1.4117831776142538
1102	20	1.2563033515561415
1102	21	1.22004518854461
1102	22	1.4785966989163626
1102	23	1.6795526737626005
1102	24	1.465366098331458
1102	25	1.367663701279369
1103	1	0.712770741148276
1103	2	0.8701578912705337
1103	3	0.8648633657706171
1103	4	1.0248525937509072
1103	5	0.5883902615261789
1103	6	0.8680996805953924
1103	7	0.7350595716418553
1103	8	0.7076616276971635
1103	9	1.07442308589156
1103	10	1.0037225085161907
1103	11	1.0438810534759426
1103	12	1.2400553701202242
1103	13	1.2461852459823533
1103	14	1.4234830610697742
1103	15	1.5804817458211744
1103	16	1.5731518490536232
1103	17	1.4240268055893068
1103	18	1.8369332322042808
1103	19	1.7585355324489385
1103	20	1.5648849243602
1103	21	1.4641610715212678
1103	22	1.7218456123718227
1103	23	1.886856343726457
1103	24	1.785203289350207
1103	25	1.679560738049183
1104	1	0.7688664498797912
1104	2	0.6415747487006165
1104	3	0.6007181675583411
1104	4	0.864157124836261
1104	5	0.6633456289425794
1104	6	0.7419933133364509
1104	7	0.9200360138738475
1104	8	0.8449335291542326
1104	9	0.8777016731521954
1104	10	0.9946412009086671
1104	11	1.0824869665362726
1104	12	0.9071830180981308
1104	13	0.9567721917086445
1104	14	1.1455146006213894
1104	15	1.2493044630189445
1104	16	1.0461490754140996
1104	17	1.0180207253838844
1104	18	1.2635593237949436
1104	19	1.2237806180199584
1104	20	1.233218681612236
1104	21	1.2175359488876438
1104	22	1.3186349732235314
1104	23	1.5404981687570398
1104	24	1.320655368368655
1104	25	1.0298906679209168
1105	1	0.710898469584226
1105	2	0.8670029411977751
1105	3	0.8199006230339023
1105	4	0.9342120698912026
1105	5	0.6671605209471281
1105	6	0.5177857536547492
1105	7	0.9300047520322394
1105	8	0.9441699823562075
1105	9	0.8487532078244229
1105	10	1.0366064339514836
1105	11	0.8808679820724592
1105	12	1.024678281455465
1105	13	0.9503867237283501
1105	14	1.0641433425690963
1105	15	1.1763251147809797
1105	16	1.2488333308138664
1105	17	1.2031959811625939
1105	18	1.0972809039389375
1105	19	1.1827508719040054
1105	20	1.1935322473568348
1105	21	1.196026380869455
1105	22	0.8070998273349375
1105	23	1.0523814578817081
1105	24	1.0150959708696021
1105	25	1.032888489065293
1106	1	0.584016362111921
1106	2	0.6069849014991746
1106	3	0.683766834547022
1106	4	0.7413351227863814
1106	5	0.7180074575772863
1106	6	0.6718676108989783
1106	7	0.4067340570627067
1106	8	0.705096577114368
1106	9	0.7390203117488525
1106	10	0.7750327987551131
1106	11	0.7927679415253763
1106	12	0.8123176151316969
1106	13	0.7861751345829546
1106	14	0.9881369160437927
1106	15	0.9462943196451263
1106	16	1.0405075453079533
1106	17	1.0298991933927937
1106	18	1.3096965950977975
1106	19	0.8339354193347077
1106	20	0.9624549244405998
1106	21	0.9194728502695426
1106	22	0.861399484374175
1106	23	1.1363674240849662
1106	24	0.9796833244965057
1106	25	1.0432633102639102
1107	1	0.696592636291534
1107	2	0.7772285554606029
1107	3	0.8893695844241662
1107	4	0.9134913686054631
1107	5	0.7811947241969293
1107	6	0.7668345560814132
1107	7	0.9553962050653837
1107	8	0.8162070013290403
1107	9	0.9045128715134112
1107	10	0.6306056939096933
1107	11	0.7814081000289335
1107	12	0.9239191054999765
1107	13	0.9186124102132496
1107	14	1.1501339246227502
1107	15	1.371929932462443
1107	16	1.1459066718634006
1107	17	1.1261258807270096
1107	18	1.4557785112309318
1107	19	1.3666655616867374
1107	20	1.2823412490413943
1107	21	1.1660269014311737
1107	22	1.128549619860614
1107	23	1.2402967348205465
1107	24	1.2851517835274109
1107	25	1.1770159666576916
1108	1	0.4962058230886299
1108	2	0.5625292647401849
1108	3	0.4371173540240889
1108	4	0.5987415752384067
1108	5	0.531563711590259
1108	6	0.6966692863419013
1108	7	0.689205839148876
1108	8	0.6769830807859236
1108	9	0.782163631181227
1108	10	0.7357334630885717
1108	11	0.6596312764188639
1108	12	0.6474723774399109
1108	13	0.6634875471676409
1108	14	0.9442171059370921
1108	15	0.9096224809536793
1108	16	0.739686562702623
1108	17	0.7926897648587854
1108	18	0.9624804664407804
1108	19	0.9066390815495851
1108	20	0.8344357473421056
1108	21	0.7907762188330949
1108	22	0.7870892581454143
1108	23	0.9846097523754196
1108	24	0.9729600739247246
1108	25	1.022889188767735
1109	1	0.163058052425626
1109	2	0.15161227126165144
1109	3	0.37267171557772016
1109	4	0.44094775844507694
1109	5	0.4568613504206809
1109	6	0.3132866542692909
1109	7	0.40695393920870515
1109	8	0.400830745835151
1109	9	0.48579419713813365
1109	10	0.5907388545611809
1109	11	0.3005638165410218
1109	12	0.502355906860366
1109	13	0.6320942207767573
1109	14	0.34535928571685304
1109	15	0.6477760123682401
1109	16	0.486715713582619
1109	17	0.394998496828741
1109	18	-1.3259956301330504
1109	19	0.08816553300048469
1109	20	0.3984428670401883
1109	21	0.5942995092562171
1109	22	0.7484340612828078
1109	23	0.2502782580512388
1109	24	0.5396878562569469
1109	25	0.6406263950986499
1110	1	0.3502920458066612
1110	2	0.24895035619887906
1110	3	0.402752418889051
1110	4	0.27355309799062155
1110	5	0.43428291197891156
1110	6	0.4348514480274943
1110	7	0.47446322979608624
1110	8	0.2940980743983914
1110	9	0.6263640198312075
1110	10	0.8246250009549527
1110	11	0.6472196672999857
1110	12	0.23592173598751032
1110	13	0.4999612683112815
1110	14	0.6840982618109237
1110	15	0.8094479483232746
1110	16	0.8002129495173165
1110	17	0.4206613178393397
1110	18	0.8379379398560403
1110	19	0.5759537260967917
1110	20	0.6504068325032364
1110	21	0.621370709359752
1110	22	0.5876722331170754
1110	23	0.5921852782224448
1110	24	0.6751543269430584
1110	25	0.6689950419084241
1111	1	0.04269011976230175
1111	2	0.2532621113904625
1111	3	0.13525441743894007
1111	4	-0.03508110231055639
1111	5	0.061161084095886054
1111	6	0.3032441491373845
1111	7	0.012823768196135177
1111	8	-0.11593092781395598
1111	9	0.18389901769322137
1111	10	0.23279814044443725
1111	11	0.08356538854410189
1111	12	0.23781106280154063
1111	13	0.26999742812090943
1111	14	0.05720196331123617
1111	15	0.33154679753036476
1111	16	0.09108794254562724
1111	17	0.17652822371228183
1111	18	0.41806166403756606
1111	19	0.2111347110565447
1111	20	0.02392744080604814
1111	21	0.26588631664005646
1111	22	-0.059623217625642155
1111	23	0.34846072487510327
1111	24	0.5775075032430479
1111	25	0.1272834466008348
1112	1	-0.39046353628991426
1112	2	-0.11601029598634227
1112	3	0.03065227027442674
1112	4	0.07463909315442407
1112	5	-0.10184084758660106
1112	6	-0.04294073126915521
1112	7	-0.3908295998309228
1112	8	-0.0064475245049848695
1112	9	0.1642155092573958
1112	10	0.28108611798501215
1112	11	0.14583180574457089
1112	12	0.09698741822364809
1112	13	-0.0964754867776918
1112	14	0.11378058390881307
1112	15	0.06855053765605135
1112	16	0.12519943395575694
1112	17	0.16436476266756728
1112	18	0.07258120820157643
1112	19	0.15524709408425252
1112	20	0.031191323198420662
1112	21	0.056694392921436076
1112	22	0.06283357159876601
1112	23	0.2520327503034778
1112	24	-0.06637588344729295
1112	25	0.15321633067094353
1113	1	-0.1726069468003395
1113	2	-0.10909856393216799
1113	3	-0.26618538811135295
1113	4	-0.028530756003277702
1113	5	-0.03740239244280019
1113	6	0.13179837018514462
1113	7	-0.08736739004368843
1113	8	0.15481656326345217
1113	9	-0.00206836396871779
1113	10	-0.3691325249569033
1113	11	-0.08339494694165239
1113	12	0.05263255467040295
1113	13	-0.0962661198735475
1113	14	0.09362260146185974
1113	15	-0.22118347852145348
1113	16	-0.05203153299530783
1113	17	-0.37550109190652137
1113	18	-0.18326275264144698
1113	19	-0.14086183055940893
1113	20	-0.30606738287075075
1113	21	-0.25601224993102534
1113	22	-0.037714570352676446
1113	23	-0.44859505375831077
1113	24	0.09585836605735665
1113	25	0.176229005184247
1114	1	-1.5012287550430017
1114	2	-1.2984565992617654
1114	3	-1.0547770141437347
1114	4	-1.3291088684297314
1114	5	-1.2173045510179463
1114	6	-1.5628458832268848
1114	7	-1.919974435446946
1114	8	-1.3629127497265259
1114	9	-1.43348954941834
1114	10	-1.4394723483772935
1114	11	-2.163248549544072
1114	12	-1.6678384610612795
1114	13	-1.6395693849777615
1114	14	-1.8837423365665842
1114	15	-1.924155423199558
1114	16	-2.613582725352188
1114	17	-4.504366363899756
1114	18	-3.6614180360100015
1114	19	-2.455498378845249
1114	20	-1.6125075461829539
1114	21	-2.570469391876319
1114	22	-2.6130520115668965
1114	23	-2.5716149161146653
1114	24	-2.2129368759132433
1114	25	-1.98943941451261
1115	1	-1.273193388197047
1115	2	-0.9285231638419091
1115	3	-0.9256845540784333
1115	4	-1.005729434118706
1115	5	-0.8514939296212134
1115	6	-1.0212996775005472
1115	7	-1.825401688381934
1115	8	-1.3443987452379602
1115	9	-1.1319145001980502
1115	10	-1.0024704976250192
1115	11	-1.676308448406651
1115	12	-1.3224114627638741
1115	13	-1.16919750993074
1115	14	-1.2918229131586756
1115	15	-2.518188454324374
1115	16	-1.2824303629519704
1115	17	-1.3718305091913336
1115	18	-1.2041794417483729
1115	19	-1.3896119448941846
1115	20	-1.2037614395337926
1115	21	-1.9194447649493185
1115	22	-1.883304785669793
1115	23	-1.3777888359015122
1115	24	-1.3656085450510531
1115	25	-1.7005846702092597
1116	1	-1.659568563382809
1116	2	-2.2650941283992942
1116	3	-1.916775201141924
1116	4	-1.5602018495227128
1116	5	-2.105779039492436
1116	6	-1.7557719386529413
1116	7	-1.4525501930227023
1116	8	-2.212469211782988
1116	9	-2.2852669386957274
1116	10	-3.225641734546681
1116	11	-1.8857071095026328
1116	12	-1.7001843559071759
1116	13	-2.393729576637952
1116	14	-2.2441670969913474
1116	15	-1.7866327606768948
1116	16	-1.6768305886000476
1116	17	-2.2400274620608513
1116	18	-2.009106458698426
1116	19	-2.305848029194898
1116	20	-2.66434321051862
1116	21	-3.1284012748082013
1116	22	-2.3196629931778787
1116	23	-2.3200544395541876
1116	24	-2.943809706786073
1116	25	-2.528214990788186
1201	1	0.7581908491746034
1201	2	0.8065422631557588
1201	3	0.9166307832919465
1201	4	1.085286906745452
1201	5	1.033020773494009
1201	6	1.0750560776282398
1201	7	0.8890211333984652
1201	8	0.4838745127203694
1201	9	0.8933638974565596
1201	10	1.1918201981101006
1201	11	0.7672918683399246
1201	12	0.05784863273450469
1201	13	0.5734219272627499
1201	14	1.1247831519017026
1201	15	0.752645357820029
1201	16	1.3245930979505616
1201	17	1.4320737131910235
1201	18	1.6994693505477443
1201	19	1.2966354008537806
1201	20	1.296184667335858
1201	21	1.2429647950651983
1201	22	1.4908672911010612
1201	23	1.742523368170204
1201	24	1.492871535937354
1201	25	1.2890625639178594
1202	1	0.5881725194804837
1202	2	0.7212355399840398
1202	3	0.4592030271476766
1202	4	0.7077632176453292
1202	5	0.6627732371363564
1202	6	0.736612390620337
1202	7	0.6055883121136745
1202	8	0.6856114579900279
1202	9	0.9849144695170295
1202	10	1.1403900374362053
1202	11	0.8257806013341735
1202	12	0.9767526859740769
1202	13	1.0305050755324032
1202	14	1.1544704680649218
1202	15	0.8418986706972547
1202	16	1.0560476914620764
1202	17	1.2997914022133799
1202	18	1.466464401543
1202	19	1.1842497936340664
1202	20	1.1433807737861272
1202	21	1.0037256223824542
1202	22	1.1699000739356649
1202	23	1.2060573073598988
1202	24	1.3720708349180626
1202	25	1.122753359848069
1203	1	0.5224373202519708
1203	2	0.7567198107305089
1203	3	0.9263877973326906
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2211	16	-0.3056396583182135
2211	17	-0.16905108994811968
2211	18	-0.1686264841275431
2211	19	-0.29750939812899446
2211	20	-0.46243867565954133
2211	21	-0.3988134384021862
2211	22	-0.6217450020780717
2211	23	-0.034845708890912874
2211	24	-0.1868961657816257
2211	25	-0.1624039079316475
2212	1	-0.04600307481732102
2212	2	-0.08638657469174049
2212	3	-0.046141105507828684
2212	4	-0.16793746975833557
2212	5	-0.0817405779539726
2212	6	-0.13856819644919938
2212	7	-0.11673938221188962
2212	8	-0.14564661996039518
2212	9	0.019090303161512168
2212	10	0.10342964452469516
2212	11	-0.20216587596139846
2212	12	-0.1077727134955282
2212	13	-0.14929956970794223
2212	14	-0.22771699304124127
2212	15	-0.342783141827276
2212	16	-0.15044458721404652
2212	17	-0.15219828673167513
2212	18	-2.947593984685952
2212	19	-1.156419754766624
2212	20	-0.47888756256297427
2212	21	-0.4605651694720976
2212	22	-0.31242979844468266
2212	23	-0.3216570036567532
2212	24	-0.7141232895996552
2212	25	-0.4435389686121667
2213	1	-0.35935408405440583
2213	2	-0.23368814841736452
2213	3	-0.4374856145738555
2213	4	-0.6190792761216599
2213	5	-0.47328607915137155
2213	6	-0.48629741300752727
2213	7	-0.31583277940655563
2213	8	-0.6742687547534203
2213	9	-0.5502220471634942
2213	10	-0.7299093907130754
2213	11	-0.7226114797930805
2213	12	-0.735667855472947
2213	13	-0.5360340890374005
2213	14	-0.7590332776803095
2213	15	-0.905355841108836
2213	16	-1.1775656956642706
2213	17	-0.820492847114842
2213	18	-0.6775589179736698
2213	19	-0.567957558393033
2213	20	-1.2786993997165794
2213	21	-0.9923689669404538
2213	22	-0.8105011292691771
2213	23	-1.1304194052989018
2213	24	-0.8880670118028746
2213	25	-1.106419570733273
2214	1	-2.0409977530398464
2214	2	-4.178427191257676
2214	3	-2.3396979534950546
2214	4	-1.9431565179204213
2214	5	-1.8026090730313307
2214	6	-2.0658484830649257
2214	7	-2.1492801508286092
2214	8	-1.8303678918652153
2214	9	-1.950571763968906
2214	10	-2.1786872825289407
2214	11	-1.8001193674275484
2214	12	-2.3918145084550453
2214	13	-2.2742026373325324
2214	14	-3.604445145244078
2214	15	-3.870336644697236
2214	16	-3.1059877886433256
2214	17	-3.5162297232314685
2214	18	-2.872183922725256
2214	19	-5.050281654641179
2214	20	-3.371336340454789
2214	21	-3.139250025213915
2214	22	-3.340616687865751
2214	23	-3.461899619690506
2214	24	-3.4409426722734686
2214	25	-3.400514298372304
2215	1	-1.2164220963232992
2215	2	-2.85965868220298
2215	3	-1.6323310221325258
2215	4	-1.235221757151315
2215	5	-1.074468321225197
2215	6	-1.5965221908162341
2215	7	-1.8870740025465125
2215	8	-1.881140576650008
2215	9	-1.6286649054632605
2215	10	-3.2056810189337916
2215	11	-2.0876812826724596
2215	12	-2.4691768971823223
2215	13	-1.7804837041529495
2215	14	-3.148541466583108
2215	15	-2.919460440243707
2215	16	-2.506198322315612
2215	17	-2.7497058875001485
2215	18	-1.6977816723736368
2215	19	-2.4120326658143156
2215	20	-2.6575354651239174
2215	21	-2.3085374429878485
2215	22	-1.9303533897378387
2215	23	-2.4921452714276278
2215	24	-2.306340895404219
2215	25	-2.3130314424524663
2216	1	-2.2719157772441427
2216	2	-2.23790724728676
2216	3	-2.7499647999065466
2216	4	-3.411276696191301
2216	5	-2.759211656552136
2216	6	-2.554417147646056
2216	7	-2.392453221263601
2216	8	-3.11196548646215
2216	9	-3.4394872604517737
2216	10	-2.7952842956829507
2216	11	-3.4731857347109276
2216	12	-3.80786455848785
2216	13	-4.449125075717529
2216	14	-3.891776173862077
2216	15	-3.849173438214173
2216	16	-2.8750132950849014
2216	17	-3.0040961100398427
2216	18	-2.9869200792391624
2216	19	-3.3054999254442734
2216	20	-3.076101636112533
2216	21	-3.451761959150513
2216	22	-4.169598650692645
2216	23	-4.139762651836406
2216	24	-3.8786689385149877
2216	25	-3.89529770250768
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	94695.0
3	6.0
4	757.0
5	4504.0
6	5667.0
7	5034.0
8	4454.0
9	4338.0
10	3802.0
11	3702.0
12	3329.0
13	3604.0
14	3401.0
15	3631.0
16	3564.0
17	3914.0
18	4461.0
19	5387.0
20	6707.0
21	8819.0
22	10854.0
23	13029.0
24	14962.0
25	17257.0
26	19174.0
27	20995.0
28	24312.0
29	28756.0
30	34561.0
31	43575.0
32	54496.0
33	69985.0
34	91145.0
35	120112.0
36	164674.0
37	253762.0
38	703450.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.61199896337249	18.528528852798896	12.415558051140291	25.443914132688324
2	15.099338675025079	20.270945186665003	40.60641907164784	24.023297066662074
3	18.558970765672242	24.82323892801366	29.789178315299345	26.828611991014757
4	12.400743305167888	16.871176044176394	38.38192819129614	32.34615245935958
5	13.185217556035855	37.84212441122526	35.86407806701053	13.108579965728358
6	31.732085909937364	37.218833381489134	18.192559687627618	12.856521020945888
7	27.655903124895755	31.82284330109687	22.529025190497148	17.992228383510227
8	25.21846373717198	36.17119162821131	20.808348112817686	17.801996521799023
9	26.437297415963947	15.267841385039882	20.010836766231474	38.2840244327647
10	14.802405378094832	28.826818588303915	34.5850044821044	21.78577155149685
11	34.15633498016166	22.649341788797763	24.54175952719419	18.65256370384639
12	22.92007666372882	24.872774153002467	31.670492320175452	20.53665686309326
13	28.699827118551045	21.91714926938352	25.711716820939913	23.671306791125527
14	21.22739536730813	21.698294946398917	27.789962967307698	29.284346718985255
15	23.327322537578237	29.818497645457757	23.5656557014039	23.288524115560104
16	22.71921380532707	28.020022911307198	26.811448766216962	22.449314517148775
17	21.974829548725847	28.078844240546825	27.44665040579537	22.49967580493196
18	21.970909094825753	27.545831181413444	28.675097704792314	21.80816201896849
19	23.319963495715697	27.137201008172568	27.531623773415152	22.011211722696583
20	23.509917382943026	27.122543791775318	27.713626487392208	21.65391233788945
21	23.348043222428913	26.738079850967534	27.189492823615318	22.72438410298824
22	23.256458378620362	27.419618663492805	27.06686410017455	22.257058857712284
23	22.708121912083914	27.229352999859024	27.34450247356136	22.718022614495702
24	22.905402169778174	26.90538925635354	27.77833676166822	22.410871812200064
25	22.913798029660025	26.89935382017702	27.48386971800861	22.702978432154346
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	354.0
1	354.0
2	511.0
3	668.0
4	668.0
5	668.0
6	1269.5
7	1871.0
8	1871.0
9	1871.0
10	2793.5
11	3716.0
12	3716.0
13	3716.0
14	6388.0
15	9060.0
16	9060.0
17	9060.0
18	16065.5
19	23071.0
20	23071.0
21	23071.0
22	34964.0
23	46857.0
24	46857.0
25	46857.0
26	65473.5
27	84090.0
28	84090.0
29	84090.0
30	107243.0
31	130396.0
32	130396.0
33	130396.0
34	149425.0
35	168454.0
36	168454.0
37	168454.0
38	185144.0
39	201834.0
40	201834.0
41	201834.0
42	214046.5
43	226259.0
44	226259.0
45	226259.0
46	233016.5
47	239774.0
48	239774.0
49	239774.0
50	233226.0
51	226678.0
52	226678.0
53	226678.0
54	206364.5
55	186051.0
56	186051.0
57	186051.0
58	161530.5
59	137010.0
60	137010.0
61	137010.0
62	112423.5
63	87837.0
64	87837.0
65	87837.0
66	68205.5
67	48574.0
68	48574.0
69	48574.0
70	35506.0
71	22438.0
72	22438.0
73	22438.0
74	15724.5
75	9011.0
76	9011.0
77	9011.0
78	6166.5
79	3322.0
80	3322.0
81	3322.0
82	2215.0
83	1108.0
84	1108.0
85	1108.0
86	714.0
87	320.0
88	320.0
89	320.0
90	205.0
91	90.0
92	90.0
93	90.0
94	51.5
95	13.0
96	13.0
97	13.0
98	16.0
99	19.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.36123999731020107
2	0.04233743527671307
3	0.03857171676417188
4	0.037011633380404814
5	0.04190706744670836
6	0.01742989711519064
7	0.01705332526393652
8	0.02383161858651066
9	0.020819043776477707
10	0.02006590007396947
11	0.0189899804989577
12	0.019850716158967118
13	0.020227288010221237
14	0.027812521014054202
15	0.02969538027032479
16	0.02173357541523771
17	0.022917086947750655
18	0.10791473337368031
19	0.024907538161522424
20	0.022648107053997713
21	0.02625243763028714
22	0.019312756371461234
23	0.022648107053997713
24	0.018505816690202407
25	0.022271535202743595
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1858875.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.23345990137152
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.99259246896979	61.608272851561374
2	11.719183582877738	17.399110891527318
3	2.9767994231254677	6.629343618330309
4	1.0225392256853647	3.03626498429976
5	0.4537981446738249	1.6843503187980584
6	0.228725737834649	1.018746173277602
7	0.14544077448488388	0.7557600330523632
8	0.09554721948872182	0.567424054928286
9	0.06087603252635528	0.40671346675498043
>10	0.2792368586485177	3.52913301533341
>50	0.015224622578573436	0.792200654970023
>100	0.008581974673554957	1.3354716596596936
>500	7.996639379131565E-4	0.42114513642431023
>1k	6.542704946562153E-4	0.8160631410825723
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3581	0.19264339990585705	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2100	0.11297155537623561	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2031	0.10925963284244503	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	5.379597875058839E-5	0.0	0.0	0.0
6	0.0	5.379597875058839E-5	0.0	0.0	0.0
7	0.0	5.379597875058839E-5	0.0	0.0	0.0
8	0.0	5.379597875058839E-5	0.0	0.0	0.0
9	0.0	5.379597875058839E-5	0.0	0.0	0.0
10	0.0	5.379597875058839E-5	0.0	5.379597875058839E-5	0.0
11	0.0	5.379597875058839E-5	0.0	5.379597875058839E-5	0.0
12	0.0	5.379597875058839E-5	0.0	5.379597875058839E-5	0.0
13	0.0	5.379597875058839E-5	0.0	5.379597875058839E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	335	0.0	12.758031	4
TAAGGCG	75	2.0790125E-4	11.397174	4
GTAGGAC	840	0.0	10.402462	3
AATCCCG	110	6.087439E-6	10.357721	19
GACTGAT	120	1.5355581E-6	10.285516	7
GGTATCA	965	0.0	10.170233	1
AGCACGG	85	6.610917E-4	10.056059	5
GTATCAA	1840	0.0	9.994486	1
AGGACGT	735	0.0	9.949645	5
TGTAGGA	985	0.0	9.931814	2
GGACGTG	735	0.0	9.817258	6
GACGTGA	445	0.0	9.814356	7
GTCCTAC	875	0.0	9.800663	1
TAGGACG	770	0.0	9.744337	4
GTCCTAG	275	0.0	9.701666	1
CTGTAGG	965	0.0	9.676533	1
GTCCTAA	580	0.0	9.528422	1
GTATAAG	260	0.0	9.528422	1
ATTGGAC	140	1.061675E-6	9.497901	3
CGTGAAA	505	0.0	9.401078	9
>>END_MODULE