FastQCFastQC Report
Thu 26 May 2016
SRR1512971_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512971_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1858875
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40870.21986416515365476No Hit
TATCAACGCAGAGTACTTTTTTTTT29390.1581063815479793No Hit
GGTATCAACGCAGAGTACTTTTTTT24920.13405957904646626No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTC604.1182037E-412.6573413
TAGGACC3600.011.6279024
TACGGTG600.005813513411.0993615
ACCGTCC600.00586035211.0874038
AGGACGT8600.010.7306125
GGACGTG8350.010.707196
GTAGGAC9250.010.68874553
GACGTGG4450.010.6792277
TGTAGGA10800.010.6508692
GTCTAAC1355.6306817E-810.5628461
GGCGTGC1002.3893632E-510.4538378
CCATCCG750.002647613410.1335069
CTGTAGG11500.010.0025561
CTGCTCG951.6439329E-410.000179
GTCCTAA5150.09.9680441
TAGGACG9000.09.9365714
GTATCAA19300.09.8021011
TCTATAC1651.0100848E-89.7949033
GGTATCA9850.09.7478451
CTAATAC2251.8189894E-129.718083
Produced by FastQC (version 0.11.5)