##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512971_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1858875
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.32686974648645	31.0	31.0	34.0	26.0	34.0
2	30.485489879631498	31.0	31.0	34.0	27.0	34.0
3	30.582272611122317	31.0	31.0	34.0	27.0	34.0
4	34.04799193060319	37.0	35.0	37.0	30.0	37.0
5	33.670616905386325	37.0	35.0	37.0	28.0	37.0
6	33.5187212695851	37.0	35.0	37.0	28.0	37.0
7	33.37784143635263	37.0	35.0	37.0	27.0	37.0
8	33.26330818371327	37.0	35.0	37.0	27.0	37.0
9	34.707579584426064	39.0	35.0	39.0	27.0	39.0
10	34.59684970748437	39.0	35.0	39.0	27.0	39.0
11	34.612325734651336	39.0	35.0	39.0	27.0	39.0
12	34.45714639230717	39.0	35.0	39.0	27.0	39.0
13	34.43289839284513	39.0	35.0	39.0	27.0	39.0
14	35.47634133548517	40.0	36.0	41.0	26.0	41.0
15	35.48726460897048	40.0	36.0	41.0	26.0	41.0
16	35.46550359760608	40.0	36.0	41.0	26.0	41.0
17	35.43593625176518	40.0	36.0	41.0	26.0	41.0
18	35.356260641517046	40.0	36.0	41.0	25.0	41.0
19	35.34464931746352	40.0	36.0	41.0	25.0	41.0
20	35.31322762423509	40.0	36.0	41.0	25.0	41.0
21	35.262601842512275	40.0	36.0	41.0	25.0	41.0
22	35.21218048550871	40.0	36.0	41.0	25.0	41.0
23	35.104100598480265	40.0	35.0	41.0	24.0	41.0
24	35.08155093806738	40.0	36.0	41.0	24.0	41.0
25	34.98626588662498	40.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.49161054747644073
1101	2	0.4674260108548616
1101	3	0.5123697904549687
1101	4	0.33366039593514074
1101	5	0.488675974939504
1101	6	0.46900892351437307
1101	7	0.45252029985471864
1101	8	0.5187793776839129
1101	9	0.4238523655650468
1101	10	0.661723097609908
1101	11	0.6562343345843829
1101	12	0.7209714482591707
1101	13	0.8199101850754644
1101	14	0.9789538788386594
1101	15	0.8502504567304996
1101	16	1.0492112119269166
1101	17	1.0199938942049087
1101	18	0.5608022098413272
1101	19	0.7635805536842639
1101	20	0.7678731987614782
1101	21	0.9298522638394715
1101	22	0.7733090735667645
1101	23	0.8220147446890564
1101	24	0.8523009567713657
1101	25	0.8027683915015373
1102	1	0.6266652325584943
1102	2	0.5575528050193768
1102	3	0.5681067889311464
1102	4	0.45846805004070745
1102	5	0.625826771007084
1102	6	0.6003664574018117
1102	7	0.6974078378335022
1102	8	0.6220315879102714
1102	9	0.7083793176540212
1102	10	0.7975812296300262
1102	11	0.7871316706054188
1102	12	0.7946171805933915
1102	13	0.7679842974820588
1102	14	0.9753681964100593
1102	15	0.8773500705752966
1102	16	0.921565401128646
1102	17	0.8799087918498003
1102	18	0.9940150448158676
1102	19	0.9611277426474913
1102	20	1.002824323755327
1102	21	0.9751073373808339
1102	22	0.8902744329352146
1102	23	0.9730516844417494
1102	24	0.9380914291187068
1102	25	0.9855596067664081
1103	1	0.729528315336907
1103	2	0.6874564186229044
1103	3	0.7548977845827736
1103	4	0.8095457494427833
1103	5	0.8848364739749925
1103	6	1.0381853346031846
1103	7	0.9471213760450325
1103	8	0.9819734293140598
1103	9	1.1466112115328642
1103	10	1.244212713123794
1103	11	1.0965308702302679
1103	12	1.1517574531186128
1103	13	1.2801743202418407
1103	14	1.3951638299100253
1103	15	1.2042856659242176
1103	16	1.363354355454483
1103	17	1.4301153550137613
1103	18	1.5074236495249096
1103	19	1.5197247578892643
1103	20	1.6070615093646552
1103	21	1.191757611305924
1103	22	1.385930630542859
1103	23	1.429697908610386
1103	24	1.4279971918709649
1103	25	1.418730767091077
1104	1	0.567541953407229
1104	2	0.614495285912124
1104	3	0.529577120362724
1104	4	0.5147629774989397
1104	5	0.6929358647432551
1104	6	0.7037764589567104
1104	7	0.7155506966850282
1104	8	0.7489298141353444
1104	9	0.9447970817168425
1104	10	0.9183138237907968
1104	11	1.0716085684728185
1104	12	0.889739478965538
1104	13	1.006466242773726
1104	14	1.1744612149372173
1104	15	1.1320018341836047
1104	16	1.109667473921597
1104	17	1.0366669580320576
1104	18	0.9691926017189942
1104	19	1.15824903631907
1104	20	1.1509902667983738
1104	21	1.0722945346250796
1104	22	1.1109807135098038
1104	23	1.0401376939864022
1104	24	1.0900363619592568
1104	25	1.288097432546543
1105	1	0.45081544715485933
1105	2	0.5872648008799182
1105	3	0.49198475583070334
1105	4	0.4763535421067431
1105	5	0.39877188217410264
1105	6	0.45489904326894504
1105	7	0.5894532165789315
1105	8	0.5844011100807762
1105	9	0.7287763352219585
1105	10	0.5868227286790173
1105	11	0.6332957605910465
1105	12	0.6125871485373509
1105	13	0.7395488498218512
1105	14	0.7813378727621512
1105	15	0.648948594320018
1105	16	0.7652827638041231
1105	17	0.47907980380697524
1105	18	0.9424319916394168
1105	19	0.9157556676188037
1105	20	1.0409173225012935
1105	21	0.9245951219687711
1105	22	0.9797903952074165
1105	23	0.8767348898939389
1105	24	0.9124686279691119
1105	25	1.1014983419819373
1106	1	0.4645210893944096
1106	2	0.45258406694012976
1106	3	0.4139962620047477
1106	4	0.3865058470959113
1106	5	0.531896433997403
1106	6	0.5113798659993449
1106	7	0.6384442089312969
1106	8	0.6777845907101607
1106	9	0.6437660154255909
1106	10	0.662017392232066
1106	11	0.48232755814095185
1106	12	0.6078289668488637
1106	13	0.5750201346012958
1106	14	0.8138550865016043
1106	15	0.7974562832487919
1106	16	0.8181032031017637
1106	17	0.8160277252057
1106	18	0.9552412804885932
1106	19	0.948882260875223
1106	20	0.9335499077158715
1106	21	0.8795283741453659
1106	22	0.882057254159875
1106	23	0.937604324524905
1106	24	0.9582721688935081
1106	25	1.0173091710571995
1107	1	0.5554695737554702
1107	2	0.5246591643689378
1107	3	0.529047929512604
1107	4	0.6060740051534239
1107	5	0.6266572713666747
1107	6	0.5984162677651739
1107	7	0.6322833699523613
1107	8	0.5808656284064284
1107	9	0.5629397925396518
1107	10	0.7973749367986542
1107	11	0.7458250246011957
1107	12	0.6064243543496275
1107	13	0.7464047883524216
1107	14	0.8676022186927668
1107	15	0.8371947327838143
1107	16	0.6301352103504101
1107	17	0.6390022260416188
1107	18	0.7034056437128484
1107	19	0.9121445638028547
1107	20	0.9869854870100525
1107	21	0.846650970268648
1107	22	0.9290995253909955
1107	23	0.8074917397358021
1107	24	0.8633310017680671
1107	25	0.7882135131201196
1108	1	0.4585094120371629
1108	2	0.4177572809199823
1108	3	0.39107753637455644
1108	4	0.439381633318483
1108	5	0.5574671580778201
1108	6	0.5234422627840161
1108	7	0.45637809799777784
1108	8	0.5998838679395391
1108	9	0.507006566786643
1108	10	0.6518545467269163
1108	11	0.6997693339345972
1108	12	0.8121680636008364
1108	13	0.8074568131721733
1108	14	0.7654711992212171
1108	15	0.7822842599948316
1108	16	0.8114309018591044
1108	17	0.6456292396258476
1108	18	0.746843983802755
1108	19	0.8063774453049248
1108	20	0.8337225400719248
1108	21	0.8776883591605369
1108	22	0.8883991289669595
1108	23	0.7704316756818841
1108	24	0.9678712514850076
1108	25	0.9527636986092247
1109	1	0.17441665655152505
1109	2	0.04340998145691444
1109	3	0.09276938786533862
1109	4	0.21025789532927774
1109	5	0.14385350509662942
1109	6	-0.17773536777455234
1109	7	0.03428395402330153
1109	8	0.20139680378101588
1109	9	0.31336314992240943
1109	10	0.07117171302800074
1109	11	0.14950493020931077
1109	12	0.3331431077296685
1109	13	0.23795574595601465
1109	14	0.42976709505500565
1109	15	0.3645946906479338
1109	16	0.3220356699888498
1109	17	0.3707241443149485
1109	18	0.3523506714444622
1109	19	0.5280675981125569
1109	20	0.41240757467335953
1109	21	0.4141103415976275
1109	22	0.393920772405977
1109	23	0.38496907994631613
1109	24	0.16417809274675932
1109	25	0.4106437420364273
1110	1	0.3079052952262522
1110	2	0.3696514997515159
1110	3	0.27025057558142507
1110	4	0.39205989950773557
1110	5	0.3074649089459882
1110	6	0.35605602387223456
1110	7	0.3236136450766054
1110	8	0.45137369286712214
1110	9	0.5670511895622496
1110	10	0.5874367120111188
1110	11	0.4065819542631459
1110	12	0.5360088610530198
1110	13	0.17355025110512656
1110	14	0.5399529992464025
1110	15	0.6950867682266519
1110	16	0.5557563422391141
1110	17	0.46251976988116894
1110	18	0.37550826938851856
1110	19	0.48175979771689725
1110	20	0.47753967665720154
1110	21	0.49768449065378206
1110	22	0.5972755526674121
1110	23	0.6447708807487231
1110	24	0.5689463365021865
1110	25	0.5879261758929957
1111	1	-0.3057587438895517
1111	2	-0.12605347710248793
1111	3	-0.05613307163268999
1111	4	-0.09193775800588355
1111	5	0.1495167225138161
1111	6	-0.06259091137474826
1111	7	0.10793153535156819
1111	8	0.2195349340130761
1111	9	-0.3124562376068738
1111	10	-0.08539044595798373
1111	11	0.06273284013200708
1111	12	0.06012299219507611
1111	13	0.09104499910193198
1111	14	0.2588709006760652
1111	15	0.40819679072195925
1111	16	0.11965648910928905
1111	17	0.2678628697714771
1111	18	0.22020607005408266
1111	19	0.20885176819412976
1111	20	0.1726427646491686
1111	21	0.04635987037826084
1111	22	0.035505023377034206
1111	23	0.1041366432652282
1111	24	0.15481380010999146
1111	25	0.2362887593208285
1112	1	0.008087727032041414
1112	2	-0.1934694526488272
1112	3	0.025633162109294005
1112	4	-0.12771636422554877
1112	5	0.001424527388678598
1112	6	0.06297825340831764
1112	7	-0.07840848651406418
1112	8	0.0453232453267276
1112	9	-0.19303888402122027
1112	10	0.09587144033136497
1112	11	-0.06108950109704381
1112	12	0.03341636145452753
1112	13	-0.00916826555350525
1112	14	0.03209135335637825
1112	15	0.04581434653208305
1112	16	-0.14858537639743474
1112	17	6.742567083009021E-4
1112	18	-0.03988227944507372
1112	19	0.06108506016761339
1112	20	0.20630226196357881
1112	21	0.25332064947463806
1112	22	0.2065386322248699
1112	23	0.41182231679128023
1112	24	0.30419277212627094
1112	25	0.34982563821115775
1113	1	-0.279473665391361
1113	2	-0.6193666896674515
1113	3	-0.22282990226002042
1113	4	-0.24685423352994462
1113	5	-0.21692358941592005
1113	6	-0.2570528081971233
1113	7	-0.12411801669016853
1113	8	0.001643376108589223
1113	9	-0.021768883777049552
1113	10	-0.22940429074705548
1113	11	0.05886278835001946
1113	12	-0.1012233300835561
1113	13	0.05362754363987676
1113	14	0.00892693062372274
1113	15	0.03266884326539099
1113	16	-0.30800931733747916
1113	17	-0.08473523186825105
1113	18	0.17952739031657217
1113	19	0.04632178396690989
1113	20	0.16185828911761746
1113	21	-0.1614404360750754
1113	22	-0.25331107086205407
1113	23	-0.0675909264697907
1113	24	-0.14129322037211978
1113	25	-0.21873888524581275
1114	1	-0.6744201968307841
1114	2	-0.4069037620386453
1114	3	-0.5120285919326442
1114	4	-0.26400748575428423
1114	5	-0.4127427605905467
1114	6	-0.87330469743479
1114	7	-0.6032963949206831
1114	8	-0.8930323361026637
1114	9	-0.7067087815131572
1114	10	-0.7657811489248658
1114	11	-0.6347237199284308
1114	12	-0.8956906847404866
1114	13	-0.7698648845918825
1114	14	-1.9323923784681014
1114	15	-1.1924530595816307
1114	16	-1.0919986122272505
1114	17	-1.358354521127353
1114	18	-1.5442440637866355
1114	19	-1.47165691979378
1114	20	-0.7110543169703476
1114	21	-0.8835710174473661
1114	22	-0.9336274369604141
1114	23	-0.8516700385109885
1114	24	-1.528858332107852
1114	25	-1.5373896219060228
1115	1	-1.603713010379355
1115	2	-1.3975265014066593
1115	3	-1.0947273568246025
1115	4	-2.262870644672443
1115	5	-1.4189424913691653
1115	6	-1.5066217108136826
1115	7	-1.001155008557916
1115	8	-1.1201250808990935
1115	9	-1.6275780679376197
1115	10	-1.4030084696860428
1115	11	-1.2667465956432977
1115	12	-1.7558606206065335
1115	13	-1.3675274272870865
1115	14	-2.120427536974212
1115	15	-2.0459762599594242
1115	16	-1.4366151685316737
1115	17	-1.4268154568278888
1115	18	-1.2968593654222218
1115	19	-1.1898449881709183
1115	20	-1.2766994239354972
1115	21	-1.3674014149250624
1115	22	-1.6693629286945324
1115	23	-2.027023434971291
1115	24	-1.5828952059453414
1115	25	-1.371418694573805
1116	1	-0.8798851022956917
1116	2	-0.982217799852684
1116	3	-0.7123879547920069
1116	4	-0.8257984725452729
1116	5	-0.8283572387050242
1116	6	-1.614835388965485
1116	7	-0.9462839629082467
1116	8	-0.9581126261829596
1116	9	-1.1236732984776765
1116	10	-1.0539739746176906
1116	11	-0.9999917851964994
1116	12	-1.6819183459681497
1116	13	-1.2808723955993955
1116	14	-1.6500723836481086
1116	15	-1.5552638598924347
1116	16	-1.809740129968766
1116	17	-1.63300810828094
1116	18	-2.3747154442580154
1116	19	-1.9813597169965789
1116	20	-1.6712621646781969
1116	21	-1.7213526802290318
1116	22	-3.1014596047925806
1116	23	-2.9119555862965356
1116	24	-2.1984517017012166
1116	25	-2.3512946983111007
1201	1	1.0153873049538369
1201	2	1.0743154133886108
1201	3	0.9691940744884491
1201	4	1.0095005658016731
1201	5	1.078181016568486
1201	6	1.0936375169868668
1201	7	1.2665926998164636
1201	8	1.2512263633937906
1201	9	1.525460847081213
1201	10	1.3534560328078484
1201	11	1.4462298992089586
1201	12	1.377016364298349
1201	13	1.508496250294094
1201	14	1.571220920533115
1201	15	1.5748589594286315
1201	16	1.551848751891832
1201	17	1.4442006456037149
1201	18	1.5472068534418995
1201	19	1.4813479230573279
1201	20	1.5916361558667091
1201	21	1.4477673934763118
1201	22	1.7275549987661805
1201	23	1.5909773867606773
1201	24	1.6323422808625168
1201	25	1.6052165133350726
1202	1	0.48500222433446183
1202	2	0.5968936881987617
1202	3	0.5701105465295555
1202	4	0.5643354548097008
1202	5	0.6392879968599772
1202	6	0.6008802981104608
1202	7	0.46003296670836136
1202	8	0.3145303613643904
1202	9	0.6063874661208146
1202	10	0.6145370883540551
1202	11	0.6747568455563808
1202	12	0.6333102706154108
1202	13	0.566782853862442
1202	14	0.5442418347229676
1202	15	0.7983476145346629
1202	16	0.6239937592380258
1202	17	0.8069592614021559
1202	18	0.7854156219835673
1202	19	0.819744877134049
1202	20	0.8918686427007501
1202	21	0.8935176159643277
1202	22	0.8753572022048886
1202	23	0.9397961644323658
1202	24	0.7515060628155226
1202	25	0.8094102936505294
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2211	9	-0.7072980071023878
2211	10	-0.8499715376607071
2211	11	-0.6101369430689303
2211	12	-0.6513610245017318
2211	13	-0.5929671190577537
2211	14	-1.7238141721626192
2211	15	-0.9398130228506858
2211	16	-0.6314767267053654
2211	17	-0.5424989961809175
2211	18	-0.7864234332387312
2211	19	-0.7887368096009411
2211	20	-0.747027687034624
2211	21	-0.7339612837467229
2211	22	-0.510004847428732
2211	23	-0.49209703575616714
2211	24	-0.7356152956829973
2211	25	-0.8173176904704604
2212	1	-0.06068032059090811
2212	2	0.010618305483422574
2212	3	0.012230894826842587
2212	4	0.24643818719138721
2212	5	0.022994112646330223
2212	6	0.03429836016826471
2212	7	-0.012482854107140895
2212	8	-0.24246813553846636
2212	9	-0.19650767131204816
2212	10	-0.1734126440563557
2212	11	-0.006713891918600723
2212	12	0.08793612388632255
2212	13	-0.057154446881447996
2212	14	-0.036307657383382264
2212	15	-0.11335464298321796
2212	16	-0.003589748818384919
2212	17	-0.28308607085890003
2212	18	-0.17798121002378053
2212	19	-0.2027994259362842
2212	20	-0.22727394568997283
2212	21	-0.16605122492757118
2212	22	-0.14548247381544854
2212	23	-0.465654477495427
2212	24	-0.2540130322625487
2212	25	-0.41287647239287395
2213	1	-0.5206914393045601
2213	2	-0.5485161454231822
2213	3	-0.44367485269282625
2213	4	-0.536939335901323
2213	5	-0.4335873105490151
2213	6	-0.30143547493266
2213	7	-0.8788017046032053
2213	8	-0.5419378584259107
2213	9	-0.7594224848597761
2213	10	-0.557594135674556
2213	11	-0.702608520724624
2213	12	-0.7071801949641738
2213	13	-0.546762726046687
2213	14	-0.4909176471199501
2213	15	-0.5442432847895802
2213	16	-0.7005021675662491
2213	17	-0.697480528607791
2213	18	-0.8144292086553264
2213	19	-1.0927840307943129
2213	20	-0.49102266276148043
2213	21	-1.2095596067270975
2213	22	-0.7715772394671774
2213	23	-0.7609247477656922
2213	24	-0.9256945238807504
2213	25	-0.9275959386946226
2214	1	-1.2313673050437153
2214	2	-1.1590475629166228
2214	3	-1.0745525658490216
2214	4	-1.5787818112881027
2214	5	-1.5955689124040333
2214	6	-3.0009546143252166
2214	7	-1.5796037724938827
2214	8	-2.153847236158075
2214	9	-1.7128759383132248
2214	10	-2.0013733579347956
2214	11	-1.7159984929753591
2214	12	-1.6834032534657126
2214	13	-1.9289898899194213
2214	14	-2.1781111697565123
2214	15	-2.4310414753852356
2214	16	-2.055958890111576
2214	17	-2.0791660115590958
2214	18	-2.0015086932031636
2214	19	-3.711421655761047
2214	20	-2.5262889416986454
2214	21	-2.895308646906514
2214	22	-2.6102608597963695
2214	23	-2.4273253255586766
2214	24	-1.8931936685950816
2214	25	-1.6968683756885738
2215	1	-1.3500502028955736
2215	2	-1.1659685297990663
2215	3	-1.207053373913947
2215	4	-1.2036147384050153
2215	5	-1.8602844298713421
2215	6	-1.692891873543708
2215	7	-1.9117097207253124
2215	8	-2.554721693444197
2215	9	-2.063021389999683
2215	10	-1.587879984067179
2215	11	-2.1701462683944612
2215	12	-2.737355782927324
2215	13	-2.328634778144391
2215	14	-2.219156500014833
2215	15	-3.3699736066673935
2215	16	-2.9268606905675867
2215	17	-2.6424396791255518
2215	18	-3.259705092291142
2215	19	-3.91662209954157
2215	20	-3.412183622447479
2215	21	-2.4243640408490847
2215	22	-3.2455855271793723
2215	23	-3.306893076342721
2215	24	-2.3849994534663637
2215	25	-2.59335089091077
2216	1	-1.077047925862054
2216	2	-0.9286177832763158
2216	3	-1.4288353117335788
2216	4	-0.8538171165982789
2216	5	-0.9947020838904592
2216	6	-1.2754259439248727
2216	7	-2.818606006861799
2216	8	-1.8663643863316217
2216	9	-1.7742129029131632
2216	10	-1.7956983314109465
2216	11	-2.297630088823233
2216	12	-1.7261909923418486
2216	13	-2.1123177677388796
2216	14	-2.31695399783748
2216	15	-2.2439307490314206
2216	16	-2.0033835286478947
2216	17	-2.2047433269581447
2216	18	-1.9453233607829148
2216	19	-2.0931824850757295
2216	20	-2.4645185269503784
2216	21	-2.209205037126978
2216	22	-2.714839351959327
2216	23	-1.7614765074191894
2216	24	-2.1004199274190185
2216	25	-2.049763674327714
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	52783.0
3	3.0
4	876.0
5	7877.0
6	8953.0
7	7004.0
8	5682.0
9	5006.0
10	4309.0
11	3795.0
12	3446.0
13	3250.0
14	3153.0
15	3127.0
16	3228.0
17	3364.0
18	3834.0
19	4574.0
20	5543.0
21	7212.0
22	9151.0
23	11040.0
24	12952.0
25	15004.0
26	17150.0
27	18476.0
28	20609.0
29	25307.0
30	31525.0
31	40431.0
32	51304.0
33	66200.0
34	86436.0
35	113734.0
36	155796.0
37	242727.0
38	804014.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.78381160448657	19.269333184025015	13.449754341157306	27.49710087033111
2	14.004829808168711	20.713356964139024	39.22053846604906	26.06127476164321
3	16.784022594311075	24.73528343756304	30.393329298634924	28.087364669490956
4	11.527885845766086	17.365655796013556	37.61912141532653	33.487336942893826
5	12.882120165006036	37.869402340295075	36.06441329242814	13.184064202270743
6	31.692443363857635	37.63679541645513	17.85125337361845	12.819507846068781
7	27.36537009601988	31.868451202495233	22.532141609816918	18.23403709166796
8	25.201696685219677	36.38146349865966	20.521870660049306	17.894969156071355
9	26.096889433286798	14.967563954495894	19.87973853113742	39.05580808107989
10	14.38832416783524	28.929586141680723	34.81898410523844	21.863105585245595
11	34.08307420836875	22.569748550155474	24.471654060102647	18.87552318137313
12	22.593890809313084	24.96364345568752	31.726579418133888	20.715886316865507
13	28.514002858835198	21.779062717378263	25.82027427387254	23.886660149914004
14	21.050686315544826	21.518254583955162	27.755403315963385	29.675655784536627
15	23.067822834713446	29.92429459259387	23.615600306436896	23.39228226625579
16	22.674603789502683	27.982339471260214	26.719405060131024	22.623651679106082
17	21.88243751745454	28.13120325613606	27.41558728776759	22.57077193864181
18	21.879318831652647	27.530294023503586	28.644898903363075	21.94548824148069
19	23.12469503682691	27.11021066465967	27.65883504202372	22.106259256489697
20	23.365122505862626	27.058132384768957	27.748466894662123	21.828278214706298
21	23.138966459785042	26.588475920625026	27.32774105600544	22.944816563584496
22	23.090036909500444	27.204093237239164	27.267303648289744	22.438566204970652
23	22.47746620940085	27.11424920987156	27.462094008472864	22.946190572254725
24	22.79319480868805	26.89088830609912	27.79358482953399	22.522332055678838
25	22.785738302422228	26.870254058343306	27.63272497410999	22.711282665124475
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	201.0
1	201.0
2	348.5
3	496.0
4	496.0
5	496.0
6	1129.5
7	1763.0
8	1763.0
9	1763.0
10	2701.5
11	3640.0
12	3640.0
13	3640.0
14	6283.5
15	8927.0
16	8927.0
17	8927.0
18	15785.5
19	22644.0
20	22644.0
21	22644.0
22	34921.5
23	47199.0
24	47199.0
25	47199.0
26	66093.5
27	84988.0
28	84988.0
29	84988.0
30	107685.0
31	130382.0
32	130382.0
33	130382.0
34	149752.5
35	169123.0
36	169123.0
37	169123.0
38	185334.5
39	201546.0
40	201546.0
41	201546.0
42	213892.5
43	226239.0
44	226239.0
45	226239.0
46	233477.5
47	240716.0
48	240716.0
49	240716.0
50	234587.5
51	228459.0
52	228459.0
53	228459.0
54	207736.5
55	187014.0
56	187014.0
57	187014.0
58	161607.5
59	136201.0
60	136201.0
61	136201.0
62	111527.0
63	86853.0
64	86853.0
65	86853.0
66	67264.5
67	47676.0
68	47676.0
69	47676.0
70	34792.5
71	21909.0
72	21909.0
73	21909.0
74	15193.5
75	8478.0
76	8478.0
77	8478.0
78	5816.0
79	3154.0
80	3154.0
81	3154.0
82	2041.0
83	928.0
84	928.0
85	928.0
86	593.5
87	259.0
88	259.0
89	259.0
90	163.0
91	67.0
92	67.0
93	67.0
94	38.5
95	10.0
96	10.0
97	10.0
98	6.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0764440858045861
2	1.075919575011768E-4
3	0.0
4	0.025660681864030664
5	0.06702978952323313
6	0.077896577230852
7	0.03303073095286128
8	0.06105843588191783
9	0.040723555914195415
10	0.10280411539237441
11	0.0029587788312823615
12	0.010759195750117679
13	0.004088494385044718
14	0.005863761683814135
15	0.004895434066303544
16	0.0143635263264071
17	0.025606885885280074
18	5.379597875058839E-4
19	0.0023670230650258894
20	0.003550534597538834
21	1.075919575011768E-4
22	2.151839150023536E-4
23	0.0
24	0.0
25	0.0026897989375294197
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1858875.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.32074704756349
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.34774432121966	60.3779813131358
2	12.07230468055363	17.70300795527979
3	3.1232216509774045	6.869908339343637
4	1.0857141288196106	3.184214840205937
5	0.48212003616419286	1.7674700609078473
6	0.2546853471393315	1.1204231948594292
7	0.14984471360547374	0.7690708439877074
8	0.0952872082798264	0.5589223436122918
9	0.07193455080577277	0.47468655032491525
>10	0.2908812690226282	3.609972131605068
>50	0.014873411511234187	0.7512411657726422
>100	0.009772984179623719	1.3935236453896211
>500	8.078488608082472E-4	0.39071635165248825
>1k	8.078488608082338E-4	1.0288612639228218
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4087	0.21986416515365476	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2939	0.1581063815479793	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2492	0.13405957904646626	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.379597875058839E-5
2	0.0	0.0	0.0	0.0	5.379597875058839E-5
3	0.0	0.0	0.0	0.0	5.379597875058839E-5
4	0.0	0.0	0.0	0.0	5.379597875058839E-5
5	0.0	1.0759195750117678E-4	0.0	0.0	5.379597875058839E-5
6	0.0	1.0759195750117678E-4	0.0	0.0	5.379597875058839E-5
7	0.0	1.0759195750117678E-4	0.0	0.0	5.379597875058839E-5
8	0.0	1.0759195750117678E-4	0.0	0.0	5.379597875058839E-5
9	0.0	1.0759195750117678E-4	0.0	0.0	5.379597875058839E-5
10	0.0	1.0759195750117678E-4	0.0	0.0	5.379597875058839E-5
11	0.0	1.0759195750117678E-4	0.0	0.0	5.379597875058839E-5
12	0.0	1.0759195750117678E-4	0.0	5.379597875058839E-5	5.379597875058839E-5
13	0.0	1.0759195750117678E-4	0.0	5.379597875058839E-5	5.379597875058839E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTTC	60	4.1182037E-4	12.65734	13
TAGGACC	360	0.0	11.627902	4
TACGGTG	60	0.0058135134	11.099361	5
ACCGTCC	60	0.005860352	11.087403	8
AGGACGT	860	0.0	10.730612	5
GGACGTG	835	0.0	10.70719	6
GTAGGAC	925	0.0	10.6887455	3
GACGTGG	445	0.0	10.679227	7
TGTAGGA	1080	0.0	10.650869	2
GTCTAAC	135	5.6306817E-8	10.562846	1
GGCGTGC	100	2.3893632E-5	10.453837	8
CCATCCG	75	0.0026476134	10.133506	9
CTGTAGG	1150	0.0	10.002556	1
CTGCTCG	95	1.6439329E-4	10.00017	9
GTCCTAA	515	0.0	9.968044	1
TAGGACG	900	0.0	9.936571	4
GTATCAA	1930	0.0	9.802101	1
TCTATAC	165	1.0100848E-8	9.794903	3
GGTATCA	985	0.0	9.747845	1
CTAATAC	225	1.8189894E-12	9.71808	3
>>END_MODULE