FastQCFastQC Report
Thu 26 May 2016
SRR1512970_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512970_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1932326
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32020.16570702873117682No Hit
GTCCTACAGTGGACATTTCTAAATT21020.1087808164874871No Hit
GGTATCAACGCAGAGTACTTTTTTT20550.10634851469162036No Hit
GTCCTAAAGTGTGTATTTCTCATTT19790.1024154309366018No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC500.001502736713.2965213
TACACCG550.00307492912.0877465
GCGTGCG658.05309E-411.6874339
ACCGTCC752.082861E-411.3949538
ACACCGT1001.9411E-611.3946586
TAGGACC5700.010.9971224
GTCCTAA6800.010.9292921
TCTATAC2400.010.6847043
GTGCGCC1106.076669E-610.35931611
CTATACT2850.010.3306294
TAATACC1303.8558028E-710.2280924
GTATCAA19350.010.0943691
GCGCCAC1054.0975643E-59.95415813
CCAACGA1151.03196835E-59.90865519
GTCCTAC11900.09.8483721
TGTAGGA13950.09.7360552
GGCGAGG6150.09.72739919
GTATTAA2650.09.7078751
CTAGACA2550.09.6837414
CTGTAGG13400.09.6703111
Produced by FastQC (version 0.11.5)