FastQCFastQC Report
Thu 26 May 2016
SRR1512970_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512970_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1932326
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40330.20871219452618245No Hit
TATCAACGCAGAGTACTTTTTTTTT27730.14350580595613782No Hit
GGTATCAACGCAGAGTACTTTTTTT25910.13408710538490917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4700.011.7423284
CTAGGAC2650.011.1236423
AACCGTT600.00586157611.0871287
GTCCTAC11500.010.9128891
GGCGAGG4850.010.76126719
TGGCGAG11900.010.44586318
TCCAACG1002.4300978E-510.43788918
TTAGACT2300.010.3427554
TCCTACA13300.010.2943052
AGGACCG856.497411E-410.0750615
TGTACCG1054.033481E-59.9684475
AGGACGT12050.09.9496455
GGCGAGA7450.09.93529619
TTAGGAC7850.09.9328853
GGACGTG11650.09.8795486
GTATAAC1356.37463E-79.859571
GGTATCA9950.09.8418731
TATGGCG13300.09.70654416
CCTACAG13550.09.68436053
GTATCAA19400.09.6054561
Produced by FastQC (version 0.11.5)