##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512970_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1932326
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.227383474631093	31.0	31.0	34.0	26.0	34.0
2	30.398946140558063	31.0	31.0	34.0	26.0	34.0
3	30.481122750508973	31.0	31.0	34.0	27.0	34.0
4	33.94309345317509	37.0	35.0	37.0	30.0	37.0
5	33.57720074148979	37.0	35.0	37.0	28.0	37.0
6	33.43618209349768	37.0	35.0	37.0	28.0	37.0
7	33.296163794308	37.0	35.0	37.0	27.0	37.0
8	33.166555746804626	37.0	35.0	37.0	26.0	37.0
9	34.60811219224913	39.0	35.0	39.0	27.0	39.0
10	34.48382933314565	39.0	35.0	39.0	27.0	39.0
11	34.51651325914985	39.0	35.0	39.0	27.0	39.0
12	34.36091529069112	39.0	35.0	39.0	26.0	39.0
13	34.34520313860084	39.0	35.0	39.0	26.0	39.0
14	35.37817117815524	40.0	36.0	41.0	25.0	41.0
15	35.37358447798146	40.0	36.0	41.0	25.0	41.0
16	35.34806497454363	40.0	36.0	41.0	25.0	41.0
17	35.315446254928	40.0	36.0	41.0	25.0	41.0
18	35.24949982559879	40.0	36.0	41.0	25.0	41.0
19	35.233510287601575	40.0	35.0	41.0	25.0	41.0
20	35.216478999920305	40.0	36.0	41.0	25.0	41.0
21	35.15657709930933	40.0	35.0	41.0	25.0	41.0
22	35.124890934552454	40.0	36.0	41.0	24.0	41.0
23	35.00603521351987	40.0	35.0	41.0	24.0	41.0
24	34.97605631761928	40.0	35.0	41.0	23.0	41.0
25	34.87160448081742	40.0	35.0	41.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6909386691737502
1101	2	0.7573736097896635
1101	3	0.7634749492756008
1101	4	0.6411313789047242
1101	5	0.8127778837708277
1101	6	0.85339320474489
1101	7	0.8459208898447699
1101	8	0.9272954317060353
1101	9	0.7986079968462718
1101	10	0.9107262821816633
1101	11	0.9612741802898981
1101	12	0.9982042419985859
1101	13	0.9860257792988492
1101	14	1.156178513942315
1101	15	1.1063177480637805
1101	16	1.2954810661046139
1101	17	1.306759695683425
1101	18	0.8034646205194775
1101	19	1.1183567207715583
1101	20	1.0029766749567557
1101	21	1.1931285635896032
1101	22	1.096933596843165
1101	23	1.1466821297048568
1101	24	1.0236695569924166
1101	25	1.1450221798146245
1102	1	0.8225599028617374
1102	2	0.8116205351273393
1102	3	0.8478562108173158
1102	4	0.8002713950844651
1102	5	0.900819213786292
1102	6	0.789475557565595
1102	7	0.8930469381996602
1102	8	0.8911018005153224
1102	9	1.1767231938533627
1102	10	1.2841159885910898
1102	11	1.1978738409932745
1102	12	1.1762453672072297
1102	13	1.292797375324227
1102	14	1.4227921868453492
1102	15	1.4177619262593666
1102	16	1.3932519999212971
1102	17	1.3974532739657093
1102	18	1.3217875769741383
1102	19	1.488795075626669
1102	20	1.3371902509178994
1102	21	1.4993881425750573
1102	22	1.4196498301727445
1102	23	1.5219769661836793
1102	24	1.5023722082166486
1102	25	1.5788537910639775
1103	1	0.7913251546572688
1103	2	0.6856338910550086
1103	3	0.7187495293850539
1103	4	0.6748691192997924
1103	5	0.7875874063138113
1103	6	0.837696625296573
1103	7	0.8150574624030966
1103	8	0.8264428585347048
1103	9	0.8824400313401384
1103	10	1.057848401799859
1103	11	1.060402672306246
1103	12	1.0965148641116542
1103	13	1.1649696769486937
1103	14	1.3724148094233328
1103	15	1.1354773590325422
1103	16	1.1390722337429366
1103	17	1.2437040264908532
1103	18	1.2584141213539226
1103	19	1.2791372361108628
1103	20	1.4054133129953428
1103	21	1.0512355763094874
1103	22	1.1422594634855088
1103	23	1.2313511377866604
1103	24	1.4202837930160186
1103	25	1.333465252734129
1104	1	0.6439724260095439
1104	2	0.7152574406842973
1104	3	0.6681068089912188
1104	4	0.6446315757486474
1104	5	0.7635129503984928
1104	6	0.7547303524385285
1104	7	0.7779736979687328
1104	8	0.6306632330985309
1104	9	0.9589759390222099
1104	10	0.8943665290500746
1104	11	0.9338816836261259
1104	12	0.8636028319722726
1104	13	0.975679460448788
1104	14	1.1288511401605774
1104	15	0.9944071312215286
1104	16	1.0328307882048335
1104	17	0.9609108262925332
1104	18	1.041119724678154
1104	19	1.1205104124591756
1104	20	1.1508842282837577
1104	21	1.0130966723452346
1104	22	1.2418513986993105
1104	23	1.0677506676392525
1104	24	1.0825246250700644
1104	25	1.1740638238955228
1105	1	0.6702001946061351
1105	2	0.8102743512659387
1105	3	0.7564612583719494
1105	4	0.648023481715903
1105	5	0.5999875341501806
1105	6	0.7763351679619603
1105	7	0.9307493767527291
1105	8	0.920402590078325
1105	9	0.9425231249802835
1105	10	0.9939302170763042
1105	11	0.962607849679209
1105	12	0.9728331766715783
1105	13	1.0441643078211769
1105	14	1.203990392676765
1105	15	1.1000027665109755
1105	16	1.1646395076323515
1105	17	0.9928251514509014
1105	18	1.2501047573398552
1105	19	1.1573730942124953
1105	20	1.2063081675814686
1105	21	1.1547830355616426
1105	22	1.1307541902352725
1105	23	1.147618315798809
1105	24	1.1604563721147727
1105	25	1.1989174738910506
1106	1	0.6592516904085208
1106	2	0.6781581055294801
1106	3	0.6280386504388815
1106	4	0.5938369168305613
1106	5	0.6900872780201581
1106	6	0.7791108837262257
1106	7	0.7172178259136359
1106	8	0.8043014570802356
1106	9	0.8093600030291626
1106	10	0.8115759040469044
1106	11	0.6397178738085785
1106	12	0.7924677455363138
1106	13	0.7398015614529001
1106	14	0.8516989664019263
1106	15	0.8773644494391775
1106	16	0.9971726442318172
1106	17	0.8636479227869032
1106	18	1.001370545715794
1106	19	0.9621780345089448
1106	20	1.075168071419661
1106	21	0.996327010307354
1106	22	1.0211744395974875
1106	23	1.1972282776866976
1106	24	1.123330170282685
1106	25	1.1376607854728888
1107	1	0.5381850134772925
1107	2	0.5013970032169652
1107	3	0.5504065630172406
1107	4	0.5424929093671409
1107	5	0.6289704296724565
1107	6	0.7309643544223405
1107	7	0.6645506017867859
1107	8	0.6272881302027713
1107	9	0.47872135321055254
1107	10	0.6938138753915624
1107	11	0.7322620042441272
1107	12	0.609132698629125
1107	13	0.6678460461677389
1107	14	0.810243073539624
1107	15	0.8448662765478474
1107	16	0.6666538264053159
1107	17	0.7506974069167569
1107	18	0.8315261702982681
1107	19	0.977361852023968
1107	20	0.9398547583689663
1107	21	0.9725306862018286
1107	22	0.8881155482004246
1107	23	0.8320367870749763
1107	24	0.9525952091723724
1107	25	0.9989229921542062
1108	1	0.5929892606733347
1108	2	0.5488589346705695
1108	3	0.510107054453627
1108	4	0.6702928428448942
1108	5	0.7328459173460331
1108	6	0.6879463981274796
1108	7	0.8055777739303096
1108	8	0.76729771446076
1108	9	0.6474667650152739
1108	10	0.8219085162792013
1108	11	0.8412773736516925
1108	12	0.9395477367089526
1108	13	0.8547835989811858
1108	14	0.9811796885838646
1108	15	0.9701341730037711
1108	16	0.8316052438035584
1108	17	1.0259421950628393
1108	18	0.9983555327024476
1108	19	1.1206512219106486
1108	20	0.9745465033426655
1108	21	1.2548672035584332
1108	22	1.1720242729335695
1108	23	1.0604511739707547
1108	24	1.2494441863150456
1108	25	1.2804545040373156
1109	1	0.22011877204998598
1109	2	0.27912108236736444
1109	3	0.3460968655931502
1109	4	0.49458311809798516
1109	5	0.38931207702157167
1109	6	0.10701063753811724
1109	7	0.34381986755663263
1109	8	0.5842825608141311
1109	9	0.7021446944735672
1109	10	0.6519999812542991
1109	11	0.6054078893869033
1109	12	0.6975160208005207
1109	13	0.6763170218700765
1109	14	0.7459798788511165
1109	15	0.729536204217041
1109	16	0.6255450111000869
1109	17	0.6548787549708237
1109	18	0.6366050126702874
1109	19	0.8875468651644454
1109	20	0.8487316688105082
1109	21	0.8094511946899203
1109	22	0.8369810475113155
1109	23	0.7499974166062486
1109	24	0.48674190093194625
1109	25	0.5231488452721962
1110	1	0.5241832253590317
1110	2	0.5221124963529391
1110	3	0.5316166117018533
1110	4	0.594146334407526
1110	5	0.49708592752354974
1110	6	0.4937030850356976
1110	7	0.5726616948299963
1110	8	0.7552271455770807
1110	9	0.7549572131424966
1110	10	0.885007020172516
1110	11	0.7426889663432377
1110	12	0.941282214654386
1110	13	0.5714356623469641
1110	14	0.8401667510746407
1110	15	1.0304953792304516
1110	16	0.9736893886802491
1110	17	0.8803239916451346
1110	18	0.7131249946618894
1110	19	0.8388776943205585
1110	20	0.8659505047068379
1110	21	0.8508939111740688
1110	22	0.8093054548632281
1110	23	0.8990369174460042
1110	24	0.7089790069751629
1110	25	0.8114149950904803
1111	1	-0.12395516500464865
1111	2	0.10581462228029537
1111	3	0.16262874719436127
1111	4	0.26408724287195895
1111	5	0.40232477639059994
1111	6	0.2015717211033774
1111	7	0.42792875976579836
1111	8	0.5517605675156858
1111	9	0.2098023078548863
1111	10	0.24307864456054062
1111	11	0.4665536647660531
1111	12	0.3710884670707273
1111	13	0.3758168915013229
1111	14	0.5260470004670807
1111	15	0.6816229578082229
1111	16	0.38920793720682667
1111	17	0.6347281676646332
1111	18	0.698534569858694
1111	19	0.7103871310509575
1111	20	0.6215650477914636
1111	21	0.4776229073535845
1111	22	0.39738938852416084
1111	23	0.5472102291983347
1111	24	0.5911123980011155
1111	25	0.7276735051126053
1112	1	0.16871773981666038
1112	2	-0.060055771254955204
1112	3	0.11572399274552936
1112	4	-0.09563975886982945
1112	5	0.13405263548368396
1112	6	0.13702747145079996
1112	7	0.13947723100601905
1112	8	0.2275119771095575
1112	9	0.0704319405564462
1112	10	0.44200900783035735
1112	11	0.2992749468828677
1112	12	0.360750528471641
1112	13	0.19778425460017957
1112	14	0.3103338585327009
1112	15	0.28454975879608213
1112	16	0.06914919637066674
1112	17	0.3491593028045301
1112	18	0.3139210000103816
1112	19	0.3226990935136982
1112	20	0.4494845701799264
1112	21	0.6633871518265053
1112	22	0.4693989632677571
1112	23	0.7106049467971403
1112	24	0.5642621949620406
1112	25	0.6218802266452457
1113	1	-0.22410131381903042
1113	2	-0.7940472596482948
1113	3	-0.3261069579493103
1113	4	-0.23266100222331687
1113	5	-0.20172436704500285
1113	6	-0.3570414059919145
1113	7	-0.012068045069263178
1113	8	0.15169497240712104
1113	9	0.009063715070197986
1113	10	-0.263785766837934
1113	11	-0.018174271329797875
1113	12	-0.031521053257279164
1113	13	0.06692785484676733
1113	14	-0.021906653794012243
1113	15	0.1666080918322237
1113	16	-0.2848586843377774
1113	17	-0.018022399279047363
1113	18	0.19161961831780872
1113	19	0.0462066753309216
1113	20	0.18647813213107156
1113	21	0.010667326677868516
1113	22	-0.13544933670353743
1113	23	0.02921820618886528
1113	24	-0.3093193454485572
1113	25	-0.23559419777689072
1114	1	-0.7528093106739142
1114	2	-0.6139448821578313
1114	3	-0.5574187836848559
1114	4	-0.3913210508993714
1114	5	-0.6163108167266529
1114	6	-0.9440206489328418
1114	7	-0.7420115105880711
1114	8	-1.135766258476373
1114	9	-0.8423929271538384
1114	10	-1.0598633739357126
1114	11	-0.9569157983491863
1114	12	-1.0208053914930844
1114	13	-0.7761539826393715
1114	14	-2.062242552557585
1114	15	-1.1407323903605686
1114	16	-1.1709514678339445
1114	17	-1.4447839642002123
1114	18	-1.4176445660793533
1114	19	-1.4081292330214126
1114	20	-0.7903318778412398
1114	21	-0.9325538965694804
1114	22	-0.9271325657808589
1114	23	-0.7455379123143118
1114	24	-1.579316930823424
1114	25	-1.4063450241540068
1115	1	-1.935459582338602
1115	2	-1.6953953014498317
1115	3	-1.4917041799956934
1115	4	-2.780383693580408
1115	5	-1.9454146086178064
1115	6	-1.9034745039841283
1115	7	-1.6492892896341225
1115	8	-1.529928796905132
1115	9	-2.1557257087871733
1115	10	-1.9909156180230099
1115	11	-1.788088940832374
1115	12	-2.1310873672639445
1115	13	-1.6955858077390644
1115	14	-2.598371768306528
1115	15	-2.591704809165549
1115	16	-1.9318927251011644
1115	17	-2.0790996088901963
1115	18	-1.9460770912673127
1115	19	-1.9281987472210318
1115	20	-1.941356898387312
1115	21	-1.9630277117461787
1115	22	-2.258122125334019
1115	23	-2.570075911127205
1115	24	-2.0988937198533506
1115	25	-1.8605377538894956
1116	1	-1.8101777194478075
1116	2	-1.9331211385182172
1116	3	-1.8460836060852017
1116	4	-1.9144959394084395
1116	5	-2.0401393638082475
1116	6	-2.6185641254853635
1116	7	-2.0249832965058303
1116	8	-2.3855486178602767
1116	9	-2.6542713160852927
1116	10	-2.6443530573407514
1116	11	-2.7259631893631173
1116	12	-3.488037739580548
1116	13	-2.8786292532627407
1116	14	-3.352388190658999
1116	15	-3.233786492932232
1116	16	-3.5526964851224037
1116	17	-3.209227135748904
1116	18	-4.200808821202045
1116	19	-3.433474388322878
1116	20	-3.241450225221449
1116	21	-3.3473965568315585
1116	22	-4.624928286886533
1116	23	-4.902804898719296
1116	24	-3.957799794617852
1116	25	-4.535583030283554
1201	1	0.8950224917637506
1201	2	0.8667514664662157
1201	3	0.8005853835408558
1201	4	0.7854351686033496
1201	5	0.9630353984331848
1201	6	0.9530561141717229
1201	7	1.0304958996766729
1201	8	1.0959754273742988
1201	9	1.2158505841200054
1201	10	1.0411111928352312
1201	11	1.1415142759388246
1201	12	0.9996527208710688
1201	13	1.0610415300679747
1201	14	1.0522097366360512
1201	15	1.2134883048890899
1201	16	1.3015309178917462
1201	17	1.1175023121130678
1201	18	1.2731551864116213
1201	19	1.0380373341944136
1201	20	1.1988100320386437
1201	21	1.2169288854884073
1201	22	1.3670818667137965
1201	23	1.242899051445562
1201	24	1.2124058608382384
1201	25	1.0802580204541101
1202	1	0.8087965071018921
1202	2	0.8746656722264774
1202	3	0.859790691612659
1202	4	0.8439455306978942
1202	5	0.9194915807046158
1202	6	1.0539283232994023
1202	7	0.9837961574471024
1202	8	0.9189444304470342
1202	9	1.1881978139208087
1202	10	1.2054027395487168
1202	11	1.2379465764739663
1202	12	1.1736807593016678
1202	13	1.2380705797618035
1202	14	1.2771110876266079
1202	15	1.4196337952479041
1202	16	1.2446525537755306
1202	17	1.445705803199715
1202	18	1.3648051898084645
1202	19	1.4457164006993537
1202	20	1.4617590262060318
1202	21	1.403071744918492
1202	22	1.4655066197721425
1202	23	1.428818952647262
1202	24	1.2200733088503526
1202	25	1.3934006345327816
1203	1	0.6947407130201633
1203	2	0.5623255411076933
1203	3	0.7043623671605772
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2211	14	-1.1968725749594213
2211	15	-0.5807951575187289
2211	16	-0.2646749878720911
2211	17	-0.17140923350249437
2211	18	-0.3403593132096603
2211	19	-0.23683689938847152
2211	20	-0.41880879331805687
2211	21	-0.4502307183908556
2211	22	-0.21732721815791933
2211	23	-0.24879923587500485
2211	24	-0.4208614823407828
2211	25	-0.45719619947837487
2212	1	0.05757151457212473
2212	2	0.19465840525060685
2212	3	0.22295121005732454
2212	4	0.2457312412417565
2212	5	0.19717199050506196
2212	6	0.3207113076795949
2212	7	0.2183984665349712
2212	8	0.05899317869577203
2212	9	0.0330961789350539
2212	10	0.04964135619142951
2212	11	0.20056995057193205
2212	12	0.2774877703827556
2212	13	0.18491436953581797
2212	14	0.36373038065127616
2212	15	0.2893220193523902
2212	16	0.3634561891074526
2212	17	0.14058155844763576
2212	18	0.3561969246501775
2212	19	0.2880383210249704
2212	20	0.17227250468609867
2212	21	0.14763492381391785
2212	22	0.18409937031412227
2212	23	-0.0487498122643899
2212	24	0.21023366007913324
2212	25	0.12453908131936231
2213	1	-0.27378414700779174
2213	2	-0.36596513584031953
2213	3	-0.3036493483329714
2213	4	-0.3029004289681154
2213	5	-0.287551610507343
2213	6	-0.06998218739438045
2213	7	-0.6778185891478046
2213	8	-0.23552587386098622
2213	9	-0.5756222643060482
2213	10	-0.29802315298644544
2213	11	-0.34671735317733976
2213	12	-0.4966748677614987
2213	13	-0.3479318058799663
2213	14	-0.48281845272125423
2213	15	-0.5243299762852871
2213	16	-0.7581394792905698
2213	17	-0.6978169020070837
2213	18	-0.9819755808093049
2213	19	-1.1527384801572538
2213	20	-0.5762975079558075
2213	21	-1.3737056968968133
2213	22	-0.8619934495779091
2213	23	-0.9612140579771591
2213	24	-1.085853346535373
2213	25	-0.8903159734175077
2214	1	-1.095278325073373
2214	2	-0.9383307147457778
2214	3	-0.7917850772901467
2214	4	-1.3173753494755545
2214	5	-1.2486633670587821
2214	6	-2.7080856004702945
2214	7	-1.4082328528025378
2214	8	-2.0565240905657625
2214	9	-1.3594636469069101
2214	10	-1.5669657299777668
2214	11	-1.5642486905585216
2214	12	-1.5081155920884157
2214	13	-1.8500222429734094
2214	14	-1.7350573849549065
2214	15	-2.086100909137059
2214	16	-1.6272905021756259
2214	17	-1.6814391231514847
2214	18	-1.6951251554014704
2214	19	-3.476894629593108
2214	20	-2.054850478366191
2214	21	-2.2726994491597736
2214	22	-2.1120820503622895
2214	23	-2.1666971550229377
2214	24	-1.5573901048633445
2214	25	-1.25006019857517
2215	1	-1.8852821167550466
2215	2	-1.700650460822338
2215	3	-1.6929657449599773
2215	4	-1.5756010332302282
2215	5	-2.4220047447985102
2215	6	-2.0956670083527023
2215	7	-2.3442709985509644
2215	8	-3.0941087178005304
2215	9	-2.6977942100074053
2215	10	-2.0157036246167053
2215	11	-2.6159634130145797
2215	12	-3.3193904794707016
2215	13	-2.761376779517825
2215	14	-2.9393138457181536
2215	15	-4.011908906962205
2215	16	-3.458256140954756
2215	17	-3.1996551726961258
2215	18	-3.7531324001366357
2215	19	-4.509747524267823
2215	20	-4.133763004985465
2215	21	-3.2476550340207098
2215	22	-4.283688554884886
2215	23	-4.209617322238483
2215	24	-3.1189965855388806
2215	25	-3.4271012083548484
2216	1	-2.301592862320444
2216	2	-2.0025331797917545
2216	3	-2.3882492539920364
2216	4	-1.9501881270125558
2216	5	-2.1719364246975132
2216	6	-2.4740347395514917
2216	7	-3.7350971832184143
2216	8	-3.021888399821254
2216	9	-2.796758008884197
2216	10	-2.8558884068376607
2216	11	-3.27486697232899
2216	12	-2.9798826943913177
2216	13	-3.248969042673515
2216	14	-3.477963635794758
2216	15	-3.563899030403679
2216	16	-3.4041191688457957
2216	17	-3.6893347273824517
2216	18	-3.428132361362458
2216	19	-3.4350227046065243
2216	20	-3.8187598797867466
2216	21	-3.6880259322943267
2216	22	-3.8425697802729744
2216	23	-3.00799997374083
2216	24	-3.18792466564323
2216	25	-3.2522324589461533
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	58657.0
3	1.0
4	956.0
5	8301.0
6	9709.0
7	7307.0
8	5892.0
9	5146.0
10	4346.0
11	3960.0
12	3680.0
13	3550.0
14	3346.0
15	3339.0
16	3432.0
17	3557.0
18	3811.0
19	4658.0
20	5774.0
21	7697.0
22	9425.0
23	11498.0
24	13546.0
25	15575.0
26	17862.0
27	19515.0
28	22113.0
29	26429.0
30	33336.0
31	42714.0
32	53512.0
33	69362.0
34	90137.0
35	117740.0
36	162037.0
37	253089.0
38	827317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.390744007449705	19.412589716047222	13.569510061559528	27.627156214943543
2	13.748367251040715	20.966618434589645	39.47692001962404	25.808094294745604
3	16.711931630584072	24.921467702654727	30.495423650046625	27.871177016714572
4	11.511365236332315	17.70768628717668	37.69675379531394	33.08419468117707
5	12.783971008533696	37.981441194458355	36.070927179033596	13.163660617974356
6	31.44817706364144	37.813874763956235	17.949632947411054	12.788315224991273
7	27.26705637279263	31.8400465493821	22.79713539078289	18.095761687042376
8	24.889690023918405	36.66237043335341	20.729368888677154	17.71857065405103
9	26.0554287104774	15.013176356077432	20.011959678797197	38.919435254647965
10	14.379135418976356	29.141532091783766	34.95479402945485	21.524538459785024
11	34.01422259851978	22.544490256831708	24.638444695041137	18.80284244960738
12	22.629741022587353	25.04923401958181	31.936251713809693	20.38477324402114
13	28.529175372070288	21.935391715992257	25.93232996710017	23.603102944837286
14	20.950481808550776	21.874299696873873	27.806504402496845	29.368714092078505
15	22.86230500186834	30.323238094646616	23.57807015637114	23.236386747113908
16	22.346571705115398	28.148508548092398	27.13588438983235	22.36903535695985
17	21.78194962891795	28.357688208251137	27.686287745569764	22.174074417261153
18	21.644904251757097	27.629357032811036	29.06380818368924	21.661930531742627
19	23.168883755014843	27.05970819722357	27.732442357773056	22.038965689988533
20	23.271341218847606	27.282913310234	27.87714744842113	21.568598022497262
21	22.920805683107844	26.755361293117147	27.346825556596905	22.977007467178108
22	22.977031248948805	27.57450754817652	27.35984342146051	22.088617781414165
23	22.43374047650345	27.352682725378635	27.516009203415987	22.69756759470193
24	22.819246244808713	27.027234031542314	27.9634119519232	22.19010777172577
25	22.603691835070155	27.162489183672623	27.758111728004042	22.47570725325318
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	217.0
1	217.0
2	340.0
3	463.0
4	463.0
5	463.0
6	1138.0
7	1813.0
8	1813.0
9	1813.0
10	2775.5
11	3738.0
12	3738.0
13	3738.0
14	6498.5
15	9259.0
16	9259.0
17	9259.0
18	16486.0
19	23713.0
20	23713.0
21	23713.0
22	36980.5
23	50248.0
24	50248.0
25	50248.0
26	70426.5
27	90605.0
28	90605.0
29	90605.0
30	115312.0
31	140019.0
32	140019.0
33	140019.0
34	160730.0
35	181441.0
36	181441.0
37	181441.0
38	198184.5
39	214928.0
40	214928.0
41	214928.0
42	226538.5
43	238149.0
44	238149.0
45	238149.0
46	245060.5
47	251972.0
48	251972.0
49	251972.0
50	244632.5
51	237293.0
52	237293.0
53	237293.0
54	213211.0
55	189129.0
56	189129.0
57	189129.0
58	162471.5
59	135814.0
60	135814.0
61	135814.0
62	110630.0
63	85446.0
64	85446.0
65	85446.0
66	65681.5
67	45917.0
68	45917.0
69	45917.0
70	33167.0
71	20417.0
72	20417.0
73	20417.0
74	14110.0
75	7803.0
76	7803.0
77	7803.0
78	5292.0
79	2781.0
80	2781.0
81	2781.0
82	1825.0
83	869.0
84	869.0
85	869.0
86	550.5
87	232.0
88	232.0
89	232.0
90	141.0
91	50.0
92	50.0
93	50.0
94	29.5
95	9.0
96	9.0
97	9.0
98	5.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07824766628405352
2	1.0350220407943587E-4
3	0.0
4	0.02432301795866743
5	0.06598265510064037
6	0.07969669714116562
7	0.03177517665238681
8	0.06091104710074802
9	0.04326392130520419
10	0.10608975918142177
11	0.002535803999946179
12	0.011747500163015972
13	0.00501985689785264
14	0.006572389959044178
15	0.006624141061083896
16	0.016353348244550866
17	0.02810084840756684
18	9.832709387546407E-4
19	0.0032085683264625117
20	0.004709350285614332
21	1.552533061191538E-4
22	2.587555101985897E-4
23	0.0
24	5.1751102039717936E-5
25	0.0032085683264625117
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1932326.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.62798105361954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.11126617063361	59.6357548372949
2	12.256499444321843	17.803296188518107
3	3.1726735937367754	6.912746329657006
4	1.0779600151344872	3.131602382229879
5	0.4852506723711929	1.7621388319615572
6	0.2544480049175865	1.108802692817146
7	0.1491424965523041	0.7582342889723183
8	0.09774906469511675	0.5679453774948776
9	0.06973630405678684	0.455832627280719
>10	0.29475585769962087	3.5853596176260014
>50	0.016750811791517963	0.8328981638058746
>100	0.011985122245154016	1.7793526319275235
>500	8.555720851812144E-4	0.43155169632223855
>1k	9.268697589463109E-4	1.2344843340919278
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4033	0.20871219452618245	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2773	0.14350580595613782	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2591	0.13408710538490917	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.5525330611915381E-4	0.0	0.0	0.0
6	0.0	1.5525330611915381E-4	0.0	0.0	0.0
7	0.0	1.5525330611915381E-4	0.0	0.0	0.0
8	0.0	1.5525330611915381E-4	0.0	0.0	0.0
9	0.0	1.5525330611915381E-4	0.0	0.0	0.0
10	0.0	1.5525330611915381E-4	0.0	1.0350220407943587E-4	0.0
11	0.0	1.5525330611915381E-4	0.0	1.5525330611915381E-4	0.0
12	0.0	1.5525330611915381E-4	0.0	1.5525330611915381E-4	0.0
13	0.0	1.5525330611915381E-4	0.0	1.5525330611915381E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	470	0.0	11.742328	4
CTAGGAC	265	0.0	11.123642	3
AACCGTT	60	0.005861576	11.087128	7
GTCCTAC	1150	0.0	10.912889	1
GGCGAGG	485	0.0	10.761267	19
TGGCGAG	1190	0.0	10.445863	18
TCCAACG	100	2.4300978E-5	10.437889	18
TTAGACT	230	0.0	10.342755	4
TCCTACA	1330	0.0	10.294305	2
AGGACCG	85	6.497411E-4	10.075061	5
TGTACCG	105	4.033481E-5	9.968447	5
AGGACGT	1205	0.0	9.949645	5
GGCGAGA	745	0.0	9.935296	19
TTAGGAC	785	0.0	9.932885	3
GGACGTG	1165	0.0	9.879548	6
GTATAAC	135	6.37463E-7	9.85957	1
GGTATCA	995	0.0	9.841873	1
TATGGCG	1330	0.0	9.706544	16
CCTACAG	1355	0.0	9.6843605	3
GTATCAA	1940	0.0	9.605456	1
>>END_MODULE