FastQCFastQC Report
Thu 26 May 2016
SRR1512969_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512969_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2170860
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT47080.21687257584551745No Hit
GTCCTAAAGTGTGTATTTCTCATTT44330.20420478520033536No Hit
CTGTAGGACGTGGAATATGGCAAGA42340.19503791124254902No Hit
CTTTAGGACGTGAAATATGGCGAGG40040.18444303179385127No Hit
GTATCAACGCAGAGTACTTTTTTTT33780.15560653381609132No Hit
GTCCTACAGTGTGCATTTCTCATTT30290.13952995587002387No Hit
CTGTAGGACCTGGAATATGGCGAGA23050.10617911795325354No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC500.001496812713.30361615
TAGGACC8450.012.2509894
CGGTACA600.005889252311.08021454
TGTAGGA27500.010.7751482
ACTGTTC6050.010.6712398
CTGTAGG27450.010.4532141
AGGACCT14800.010.3957225
GGACCTG14200.010.3632186
AATGTCC7150.010.3573798
GTCCTAC24950.010.2015551
GGCGAGG11100.010.178319
GTAGGAC27600.010.1511293
AGGACGT25550.010.0363145
GGACGTG25050.010.00576
TGGCGAG23750.09.92246718
AATGCCG2300.09.9155118
TTAGGAC15700.09.8602823
TATACAC9250.09.8566853
GGTATCA10450.09.8521991
TAGGACA8800.09.8210994
Produced by FastQC (version 0.11.5)