##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512969_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2170860
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.092358328035893	31.0	30.0	34.0	23.0	34.0
2	29.131554314879818	31.0	30.0	34.0	22.0	34.0
3	29.28282155459127	31.0	30.0	34.0	23.0	34.0
4	32.70024183963959	35.0	33.0	37.0	25.0	37.0
5	32.80753203799416	35.0	33.0	37.0	26.0	37.0
6	32.825339727112755	37.0	35.0	37.0	26.0	37.0
7	32.637831089982775	36.0	33.0	37.0	25.0	37.0
8	32.65038279760095	36.0	33.0	37.0	25.0	37.0
9	33.983114526040374	38.0	34.0	39.0	25.0	39.0
10	33.81380927374405	38.0	34.0	39.0	23.0	39.0
11	33.92009664372645	38.0	34.0	39.0	24.0	39.0
12	33.807706162534664	38.0	34.0	39.0	23.0	39.0
13	33.83049068111256	38.0	34.0	39.0	23.0	39.0
14	34.834660917792945	39.0	34.0	41.0	22.0	41.0
15	34.795515141464676	39.0	34.0	41.0	22.0	41.0
16	34.695107468929365	39.0	34.0	41.0	19.0	41.0
17	34.656599688602675	39.0	34.0	41.0	19.0	41.0
18	34.35114793215592	39.0	34.0	41.0	17.0	41.0
19	34.58939037984946	39.0	34.0	41.0	18.0	41.0
20	34.66985388279299	39.0	34.0	41.0	18.0	41.0
21	34.60301585546742	39.0	34.0	41.0	18.0	41.0
22	34.52580866569009	39.0	34.0	41.0	18.0	41.0
23	34.26450254737754	39.0	34.0	41.0	16.0	41.0
24	34.26818864413182	39.0	34.0	41.0	11.0	41.0
25	34.192602010263215	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6956554208677055
1101	2	1.0274495533125219
1101	3	0.999995098056818
1101	4	0.9744957669020025
1101	5	0.824729824047516
1101	6	1.0442295964641346
1101	7	0.9952103607100966
1101	8	0.9231046721113074
1101	9	0.8951387270704032
1101	10	0.9724048969662036
1101	11	1.1581811946905702
1101	12	1.2262678772735072
1101	13	1.1600979113424827
1101	14	1.2899804614207326
1101	15	1.075179843445305
1101	16	1.5306704820798842
1101	17	1.4791117990912426
1101	18	1.7366704407381661
1101	19	1.233212172764496
1101	20	1.4290110936819929
1101	21	1.4318854356983977
1101	22	1.422179440690023
1101	23	1.7417539956772714
1101	24	1.389260013241568
1101	25	1.6181990489329436
1102	1	0.6322228331331488
1102	2	0.7445495312358545
1102	3	0.6527906251253484
1102	4	0.8288452196320364
1102	5	0.7372838081148458
1102	6	0.6448283113154858
1102	7	0.8261181379054534
1102	8	0.8474041052074313
1102	9	0.8605160085251597
1102	10	1.086152270081378
1102	11	0.8780021863333971
1102	12	0.9520499368393729
1102	13	0.8856035533823388
1102	14	0.9777399587044968
1102	15	1.0971198646991525
1102	16	1.1930827677277378
1102	17	0.9168497156046982
1102	18	1.3522811067446128
1102	19	1.3094721867010861
1102	20	1.1719112039184054
1102	21	1.0109208285290237
1102	22	1.27518978366448
1102	23	1.4202657196373636
1102	24	1.34807269986932
1102	25	1.2859269997221716
1103	1	0.4688626609102293
1103	2	0.7292657569624446
1103	3	0.6844805589365421
1103	4	0.8770958821874402
1103	5	0.2893673180514682
1103	6	0.8326535243847175
1103	7	0.5049927658662057
1103	8	0.5996608697294477
1103	9	0.9904428794348021
1103	10	0.9489831915699583
1103	11	1.0032706984758235
1103	12	1.1751688728837877
1103	13	1.0779253415803254
1103	14	1.1893464648235934
1103	15	1.3820320343999057
1103	16	1.351715863718276
1103	17	1.2025150720102644
1103	18	1.5263621053374408
1103	19	1.5547850603861164
1103	20	1.3609543934765256
1103	21	1.2010948803091708
1103	22	1.3308289264312378
1103	23	1.5616965435146781
1103	24	1.4026919023066426
1103	25	1.4089550699950735
1104	1	0.5405883988980236
1104	2	0.570998598042685
1104	3	0.5846299233629431
1104	4	0.919791024869113
1104	5	0.6366732725375286
1104	6	0.6626723187256118
1104	7	0.8617274165607753
1104	8	0.7334273468378285
1104	9	0.8501760396911138
1104	10	0.8980765676690865
1104	11	0.8565168544702786
1104	12	0.8433392174372045
1104	13	0.8430128228964122
1104	14	1.0700364194692469
1104	15	1.1674019794132775
1104	16	1.0225466619291836
1104	17	1.0064855724469979
1104	18	1.2550503344374633
1104	19	1.1513913923263814
1104	20	1.144793468891848
1104	21	1.1641160527967642
1104	22	1.1777669022565007
1104	23	1.436680335354886
1104	24	1.2920386172866856
1104	25	1.178421604241663
1105	1	0.8584546628873007
1105	2	0.9189764435482211
1105	3	0.8888023309725774
1105	4	1.043951422904712
1105	5	0.7076840380752003
1105	6	0.6018322159245031
1105	7	0.8645994349187163
1105	8	0.8496734741091956
1105	9	0.7377075521269205
1105	10	0.9202752793629614
1105	11	0.9645157246106706
1105	12	0.9562560939532929
1105	13	1.033902533048547
1105	14	1.1143237813626499
1105	15	1.3142503680849842
1105	16	1.2497540384484154
1105	17	1.1187643199489443
1105	18	1.1884316967273278
1105	19	1.2390192545381495
1105	20	1.2622440559215065
1105	21	1.1721423989092514
1105	22	0.9576418121584283
1105	23	1.157086489412258
1105	24	1.2552360499206472
1105	25	1.2864951620946101
1106	1	0.5774134221739828
1106	2	0.6267223254552547
1106	3	0.6302023204769327
1106	4	0.6054779982004703
1106	5	0.6982170344758174
1106	6	0.6939996368684689
1106	7	0.4251829582586879
1106	8	0.6339052961300098
1106	9	0.7700724416490061
1106	10	0.8496254026176331
1106	11	0.789019617293647
1106	12	0.7512171636653875
1106	13	0.709814618957914
1106	14	0.8572169364636579
1106	15	1.028088047471698
1106	16	0.9202534314905648
1106	17	0.9133941802695276
1106	18	1.1917275370883829
1106	19	0.821719888020418
1106	20	0.9082036947156595
1106	21	0.8559231934504083
1106	22	0.8662032939499937
1106	23	1.1941986270909268
1106	24	1.1284719881540255
1106	25	1.1758025800005427
1107	1	0.25122669201365255
1107	2	0.34622108005942565
1107	3	0.5495140683076478
1107	4	0.5700557263610904
1107	5	0.46241759070762356
1107	6	0.3146290667219489
1107	7	0.40426530058602594
1107	8	0.37589580519239973
1107	9	0.5088426747146713
1107	10	0.25086929566973026
1107	11	0.3457455571096588
1107	12	0.6406748160044629
1107	13	0.4931733084943275
1107	14	0.6886436514331891
1107	15	0.8495793589923295
1107	16	0.657610288656052
1107	17	0.7089454974803431
1107	18	1.0988430913296412
1107	19	0.9694376641833458
1107	20	0.8334116830785518
1107	21	0.803641529971415
1107	22	0.7897645936852769
1107	23	0.9476823350618417
1107	24	0.7512638907760163
1107	25	0.933563093655323
1108	1	0.7437198228878366
1108	2	0.6662959472441123
1108	3	0.5586073437003485
1108	4	0.8171967061859533
1108	5	0.8023447685648151
1108	6	0.8662129999536674
1108	7	0.9658513704535139
1108	8	0.93292940866111
1108	9	1.0728068551732903
1108	10	1.0743315549047665
1108	11	0.8360985549861795
1108	12	1.0997474994241543
1108	13	1.0482878533217956
1108	14	1.2142050928679708
1108	15	1.2316932694904423
1108	16	1.2002052802685483
1108	17	1.2137898153364688
1108	18	1.2997609620813293
1108	19	1.2564501327770472
1108	20	1.2633804062613123
1108	21	1.1008476497092872
1108	22	1.1718307088234639
1108	23	1.2745144169727638
1108	24	1.3296448670903374
1108	25	1.3944192767511865
1109	1	0.281474788834295
1109	2	0.09502494778162429
1109	3	0.3146987460683448
1109	4	0.3895232918104057
1109	5	0.3434362245498903
1109	6	0.28199984393199884
1109	7	0.4295202729522032
1109	8	0.36760186195058964
1109	9	0.640989343568549
1109	10	0.737663742173126
1109	11	0.26401592705248333
1109	12	0.6116917081768705
1109	13	0.5126901368730117
1109	14	0.4488422929306992
1109	15	0.6818773101000133
1109	16	0.6976562910174309
1109	17	0.43233921207735904
1109	18	-1.195907951140775
1109	19	0.3011880525149664
1109	20	0.47168609329040834
1109	21	0.6858287027754457
1109	22	0.7681638370714836
1109	23	0.3851629691106808
1109	24	0.6572453622440264
1109	25	0.6496585027351003
1110	1	0.19714132950083396
1110	2	0.27641670630104187
1110	3	0.3904072286917213
1110	4	0.07337841981447468
1110	5	0.30493246264187235
1110	6	0.3003705617707979
1110	7	0.4376742571700163
1110	8	0.26357920739602037
1110	9	0.5995463606362037
1110	10	0.760645528452784
1110	11	0.660135357746384
1110	12	0.09485963283415799
1110	13	0.2662291989227157
1110	14	0.3254759381813699
1110	15	0.5683810210718079
1110	16	0.6705905591644665
1110	17	0.18935048238650154
1110	18	0.7143670575384888
1110	19	0.5845122374348932
1110	20	0.5460141037886288
1110	21	0.48296380842118936
1110	22	0.502192985994256
1110	23	0.35760814900266524
1110	24	0.590714447308855
1110	25	0.6022973676080028
1111	1	0.31577651702955123
1111	2	0.646882710756298
1111	3	0.591415157701249
1111	4	0.5130246165121548
1111	5	0.47671317724228857
1111	6	0.7221340833877434
1111	7	0.6476934532861378
1111	8	0.5427757659867041
1111	9	0.9437160184246594
1111	10	0.8202081115129047
1111	11	0.7666250958933887
1111	12	0.8501531423585575
1111	13	0.8928889220822356
1111	14	0.8000033969623672
1111	15	1.0040772048468796
1111	16	0.8348898820610486
1111	17	0.8015807047530856
1111	18	0.907620479370955
1111	19	0.7747113107459498
1111	20	0.6951101987604389
1111	21	0.9331579219209516
1111	22	0.7693936441468026
1111	23	1.0673101538674672
1111	24	1.2429322049767961
1111	25	0.6068612686539367
1112	1	-0.3641327012554072
1112	2	-0.07029171655875999
1112	3	0.006482603422060862
1112	4	-0.05882219887670459
1112	5	-0.06985817558280871
1112	6	-0.17526811951907462
1112	7	-0.43522807049877343
1112	8	-0.14741976910373467
1112	9	0.04022676124681368
1112	10	0.03816471887456174
1112	11	-0.046516046518249254
1112	12	0.10731779166815869
1112	13	-0.14707252838574902
1112	14	-0.08257288575812538
1112	15	-0.0464464585043487
1112	16	0.2027602050380395
1112	17	0.1829217098033311
1112	18	0.09253900591335906
1112	19	0.013058346428941547
1112	20	-0.15762418759638308
1112	21	0.026189768765668475
1112	22	-0.028085521515158973
1112	23	0.20720085312426306
1112	24	-0.11193036849520155
1112	25	0.07776247137276471
1113	1	-0.36138309407751024
1113	2	-0.28206045345854136
1113	3	-0.42718987876256875
1113	4	-0.2368022067462192
1113	5	-0.4134421346310049
1113	6	-0.3218733667978171
1113	7	-0.4229841278252948
1113	8	-0.1870419179555043
1113	9	-0.39555001816754753
1113	10	-0.5367266032242171
1113	11	-0.44417982890631436
1113	12	-0.13519700497531772
1113	13	-0.3475978312552357
1113	14	-0.2546598665245341
1113	15	-0.6986319843556572
1113	16	-0.7400020503497373
1113	17	-0.7221086304716238
1113	18	-0.7481803047465618
1113	19	-0.7440225054105412
1113	20	-0.6716712294606779
1113	21	-0.5696959629794733
1113	22	-0.49668449831022343
1113	23	-0.8798767856550995
1113	24	-0.26903804782691054
1113	25	-0.3801165531620825
1114	1	-1.388918414453645
1114	2	-1.1321535036202945
1114	3	-1.1245033733537895
1114	4	-1.2396037287100548
1114	5	-1.1994932359809525
1114	6	-1.657602005182362
1114	7	-1.7846249792646596
1114	8	-1.2754161424781145
1114	9	-1.3810138904709248
1114	10	-1.5739562239981808
1114	11	-2.161671809139996
1114	12	-1.585063291209707
1114	13	-1.5715316522668061
1114	14	-1.8910438419295517
1114	15	-1.847679055306223
1114	16	-2.6286998736691913
1114	17	-4.740518236772324
1114	18	-3.5116715303858044
1114	19	-2.6564524160185137
1114	20	-1.5856467090812245
1114	21	-2.625724138975844
1114	22	-2.659141673064049
1114	23	-2.678163767425719
1114	24	-2.1556602754027665
1114	25	-2.028765496331701
1115	1	-1.1719313359790178
1115	2	-0.8644843552905535
1115	3	-0.8378845488802185
1115	4	-0.9820025376495529
1115	5	-0.8620032461590448
1115	6	-1.0197327049562404
1115	7	-2.121892635496632
1115	8	-1.4939006574860656
1115	9	-1.1966521566478008
1115	10	-0.962294732485951
1115	11	-1.5923932825004385
1115	12	-1.3990616103608744
1115	13	-1.0812073827730089
1115	14	-1.221775562267993
1115	15	-2.637140598139453
1115	16	-1.3468738794109427
1115	17	-1.4418435412970112
1115	18	-1.3997621308931585
1115	19	-1.5200104595518127
1115	20	-1.3495790686933162
1115	21	-2.048264648982574
1115	22	-2.0359998634993985
1115	23	-1.4074504055386257
1115	24	-1.332039355034702
1115	25	-1.7064959715331511
1116	1	-1.1917959686751516
1116	2	-2.010089379325386
1116	3	-1.4602276167407062
1116	4	-1.196225432958908
1116	5	-1.7275311445751633
1116	6	-1.472742129077382
1116	7	-1.1784089715592678
1116	8	-1.8126887112890522
1116	9	-1.7816775542405878
1116	10	-2.7475114840275694
1116	11	-1.3725902983846794
1116	12	-1.2590830738008378
1116	13	-1.9593128894513363
1116	14	-1.7929836473170724
1116	15	-1.3518279668320332
1116	16	-1.2676934161705304
1116	17	-1.5763760747129751
1116	18	-1.4285740816326964
1116	19	-1.7030349551285227
1116	20	-2.091176448304182
1116	21	-2.4817063269920396
1116	22	-1.7105922122616448
1116	23	-1.6771474514359923
1116	24	-2.2421584828046335
1116	25	-1.970504556909134
1201	1	0.37471443641013025
1201	2	0.3485759114503608
1201	3	0.45252710257978634
1201	4	0.5405131834319903
1201	5	0.6118690764945143
1201	6	0.6188292350975786
1201	7	0.4657259480765106
1201	8	0.18736391645568062
1201	9	0.40857953353560816
1201	10	0.7139183048837907
1201	11	0.35346190220509754
1201	12	-0.2658713069664458
1201	13	0.20659437681579362
1201	14	0.6349257490891063
1201	15	0.39923293805521354
1201	16	0.8011312421904577
1201	17	0.9858970924553674
1201	18	1.311289794012005
1201	19	0.833112141987236
1201	20	0.9259495314938846
1201	21	0.754676699888968
1201	22	0.9433776311289606
1201	23	1.2483388724364772
1201	24	1.0485650525465857
1201	25	0.9782637839635058
1202	1	0.824005297541273
1202	2	0.8985660145579857
1202	3	0.5843442965962495
1202	4	1.0896844052068175
1202	5	0.9556808426768555
1202	6	0.9332784672694459
1202	7	1.0671419649856588
1202	8	0.9902297242843332
1202	9	1.3541457949602957
1202	10	1.3308181367981078
1202	11	0.9608793577394437
1202	12	1.176911782220472
1202	13	1.230693701935401
1202	14	1.4603773265245579
1202	15	0.9884521078512805
1202	16	1.2370605142451865
1202	17	1.4201117336734086
1202	18	1.716902277845925
1202	19	1.3745700626616824
1202	20	1.271535263727813
1202	21	1.3915602261540272
1202	22	1.4519428032033943
1202	23	1.5066973171156164
1202	24	1.5630762960161348
1202	25	1.4223549239609312
1203	1	0.3856339458285021
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2211	14	0.18435340344181128
2211	15	0.3013366795593839
2211	16	0.08097229933343897
2211	17	0.17656683130497441
2211	18	0.09663171639889612
2211	19	-0.11123623490932033
2211	20	-0.3007621791578643
2211	21	-0.16294864600190095
2211	22	-0.2897962208885687
2211	23	0.39293222687993534
2211	24	0.08430366430232539
2211	25	0.2046676827442795
2212	1	-0.42451142924869956
2212	2	-0.3723712313081009
2212	3	-0.36479581850686316
2212	4	-0.4883824046436729
2212	5	-0.42212079907164934
2212	6	-0.5614450176580235
2212	7	-0.5449313506929698
2212	8	-0.5931376673373308
2212	9	-0.4936126136140544
2212	10	-0.4194989875702504
2212	11	-0.6761380050369326
2212	12	-0.5179145726390857
2212	13	-0.5599133349240049
2212	14	-0.6465965424274884
2212	15	-0.7671159009899711
2212	16	-0.54334867176172
2212	17	-0.4989908825591911
2212	18	-3.0968705637969336
2212	19	-1.4071365529870619
2212	20	-0.8470469210071201
2212	21	-0.9218311105295314
2212	22	-0.6348357171377472
2212	23	-0.7355006414722851
2212	24	-1.1589599378322433
2212	25	-0.8364219490711591
2213	1	-0.033775049433167226
2213	2	0.02276053818241408
2213	3	-0.28070111838438194
2213	4	-0.4060465201690917
2213	5	-0.2591894904106269
2213	6	-0.33532838584116575
2213	7	-0.17883513815650787
2213	8	-0.46192408200266755
2213	9	-0.4682314649914687
2213	10	-0.48632992201877556
2213	11	-0.42647753689912804
2213	12	-0.48919807751467914
2213	13	-0.230774328177354
2213	14	-0.3368638878195327
2213	15	-0.48471285450777657
2213	16	-0.9467947425305141
2213	17	-0.6744404619930648
2213	18	-0.4026198515751389
2213	19	-0.31354629536916434
2213	20	-1.079912234959906
2213	21	-0.7215247367365833
2213	22	-0.4596644286422418
2213	23	-0.7732769023247883
2213	24	-0.5019664611748453
2213	25	-0.7557076802742557
2214	1	-1.7881548475051865
2214	2	-4.140507438657497
2214	3	-2.1249235157498916
2214	4	-1.6492882344668303
2214	5	-1.6862185157113743
2214	6	-1.7579992076107906
2214	7	-1.8308675536882895
2214	8	-1.6197203378449423
2214	9	-1.9241886715485137
2214	10	-1.9826729033006742
2214	11	-1.6612402274401816
2214	12	-2.276102073780276
2214	13	-2.054164729280849
2214	14	-3.235126702109614
2214	15	-3.551781873345824
2214	16	-2.71150894941114
2214	17	-3.1286155930484334
2214	18	-2.509170135974127
2214	19	-4.4835516794588415
2214	20	-2.852210623626732
2214	21	-2.8440324246169233
2214	22	-2.960848794105118
2214	23	-2.9830361131237595
2214	24	-3.176291782821618
2214	25	-3.0696010932008164
2215	1	-1.128849154165998
2215	2	-2.825319058143293
2215	3	-1.5366209231973826
2215	4	-1.1547925237709649
2215	5	-1.1720586540155296
2215	6	-1.6138392754548398
2215	7	-1.888402295489037
2215	8	-1.8786942002168452
2215	9	-1.656953723046719
2215	10	-3.17553422859746
2215	11	-2.288603825256061
2215	12	-2.5804719373062746
2215	13	-1.8481827385226843
2215	14	-3.2884199261779905
2215	15	-2.900691918734779
2215	16	-2.514298248219262
2215	17	-2.757883601070766
2215	18	-1.727648858919025
2215	19	-2.4269715817650024
2215	20	-2.763293311813481
2215	21	-2.3203649652439466
2215	22	-1.891376484294561
2215	23	-2.4009606582992546
2215	24	-2.3521043820642262
2215	25	-2.3151241212798936
2216	1	-1.8912575957401963
2216	2	-1.8223318182593502
2216	3	-2.397300804089099
2216	4	-2.9631297568952704
2216	5	-2.2063286304781258
2216	6	-2.1681954286316945
2216	7	-2.1068280278697813
2216	8	-2.595788110649913
2216	9	-2.8000628790626223
2216	10	-2.378768801890999
2216	11	-2.9733629221684694
2216	12	-3.3005603672331
2216	13	-3.977302911014771
2216	14	-3.279108265283014
2216	15	-3.1214351668966778
2216	16	-2.305638805698713
2216	17	-2.4913235286906428
2216	18	-2.564667408058124
2216	19	-2.8071186992128396
2216	20	-2.649164109138745
2216	21	-2.937405665195765
2216	22	-3.6102968834944953
2216	23	-3.4330290727655743
2216	24	-3.611450707767837
2216	25	-3.619610094165079
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	105429.0
3	1.0
4	829.0
5	5056.0
6	6368.0
7	5800.0
8	4954.0
9	4756.0
10	4438.0
11	4083.0
12	4007.0
13	3978.0
14	3918.0
15	4076.0
16	4060.0
17	4398.0
18	5072.0
19	6084.0
20	7842.0
21	9847.0
22	12431.0
23	15081.0
24	17528.0
25	19818.0
26	22500.0
27	24732.0
28	27968.0
29	33384.0
30	41267.0
31	51055.0
32	64800.0
33	82858.0
34	107722.0
35	142587.0
36	196715.0
37	305800.0
38	809618.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.893769676348626	19.013420367707905	12.553152145270975	25.5396578106725
2	14.277169284763671	20.424981474859262	42.297135884211414	23.000713356165647
3	18.327395083756045	25.408780744956942	29.97177104223192	26.29205312905509
4	11.998910647772888	17.308535056267072	39.194054459316796	31.498499836643244
5	12.55236758391832	38.54060627733587	36.43106419041132	12.47596194833449
6	30.929355903716814	37.96690553481802	18.656778993362863	12.446959568102303
7	27.12673789079939	31.91646790468086	23.17832739542939	17.77846680909036
8	24.213306327322254	37.90629387190544	21.132602985374458	16.74779681539785
9	26.36483803656277	15.118046459297318	20.57468028652004	37.94243521761987
10	14.618882633174534	29.158519392202574	35.34352164978852	20.879076324834365
11	33.6517104225918	22.339594754541263	25.813239148633464	18.19545567423348
12	23.11377433111323	24.791520001179467	32.61829684971748	19.47640881798982
13	28.896038376870674	22.463894983307796	25.995888455701383	22.64417818412015
14	20.283517231878044	22.78035063209893	28.104108677612267	28.83202345841076
15	22.37636508820747	32.03073703359151	23.459082026705296	22.13381585149573
16	21.508011944083407	28.26910769993462	28.73956378570866	21.48331657027331
17	21.19114766742336	29.120936080769955	28.462996179064703	21.22492007274198
18	21.036505647558933	27.637967791874324	30.72200119993046	20.603525360636286
19	23.14194248137865	27.150497751983778	27.842180875228877	21.8653788914087
20	23.332219875000863	27.819288016347105	28.383097241771736	20.465394866880295
21	22.485370955702795	27.11464563156003	27.405567791221653	22.994415621515525
22	22.838243715989492	28.82989805429149	27.489634728715313	20.8422235010037
23	21.775709158998037	28.429527331768966	28.05711144786062	21.737652061372366
24	22.80811358951606	27.22874458300046	28.79291327917025	21.170228548313233
25	22.323067899667485	27.847106301398387	28.299552667844008	21.530273131090112
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	248.0
1	248.0
2	423.5
3	599.0
4	599.0
5	599.0
6	1198.5
7	1798.0
8	1798.0
9	1798.0
10	2821.5
11	3845.0
12	3845.0
13	3845.0
14	6922.0
15	9999.0
16	9999.0
17	9999.0
18	18608.5
19	27218.0
20	27218.0
21	27218.0
22	43965.5
23	60713.0
24	60713.0
25	60713.0
26	87513.5
27	114314.0
28	114314.0
29	114314.0
30	148097.0
31	181880.0
32	181880.0
33	181880.0
34	204240.0
35	226600.0
36	226600.0
37	226600.0
38	241032.5
39	255465.0
40	255465.0
41	255465.0
42	265415.0
43	275365.0
44	275365.0
45	275365.0
46	277389.5
47	279414.0
48	279414.0
49	279414.0
50	265307.0
51	251200.0
52	251200.0
53	251200.0
54	220644.0
55	190088.0
56	190088.0
57	190088.0
58	161209.5
59	132331.0
60	132331.0
61	132331.0
62	107044.0
63	81757.0
64	81757.0
65	81757.0
66	63256.5
67	44756.0
68	44756.0
69	44756.0
70	32656.0
71	20556.0
72	20556.0
73	20556.0
74	14358.5
75	8161.0
76	8161.0
77	8161.0
78	5615.0
79	3069.0
80	3069.0
81	3069.0
82	2061.5
83	1054.0
84	1054.0
85	1054.0
86	684.5
87	315.0
88	315.0
89	315.0
90	197.5
91	80.0
92	80.0
93	80.0
94	51.0
95	22.0
96	22.0
97	22.0
98	17.5
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35608007886275483
2	0.03851008356135357
3	0.0344563905548953
4	0.03514736095372341
5	0.039753830279244166
6	0.015938383866301836
7	0.015708060400025797
8	0.022064988069244448
9	0.01847194199533825
10	0.018425877302083046
11	0.01718213058419244
12	0.017873100983020553
13	0.01778097159651014
14	0.025381645983619396
15	0.02667145739476521
16	0.019024718314400745
17	0.02068304727158822
18	0.11060132850575348
19	0.02312447601411422
20	0.02049878849856739
21	0.02395364049270796
22	0.018011295062786176
23	0.020130270952525727
24	0.017412454050468477
25	0.020130270952525727
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2170860.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.80753255588887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.81890334330242	60.1882863062309
2	11.12936104914373	15.9834391172526
3	2.7315927291201487	5.884468014771731
4	0.9636360185317725	2.7678529909098954
5	0.4388053936560081	1.5754766295326719
6	0.22908312819362608	0.98699365114612
7	0.14657214889270617	0.736748904737971
8	0.09860864163480866	0.56646745955868
9	0.07468597163303256	0.4826713805556459
>10	0.32964852359672353	4.139714476034488
>50	0.020180932016499637	1.0276098281570551
>100	0.015385190972339141	2.324614360599573
>500	0.00225077319484699	1.2281922343878597
>1k	0.0012861561113408006	2.107464646124871
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	4708	0.21687257584551745	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4433	0.20420478520033536	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4234	0.19503791124254902	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4004	0.18444303179385127	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3378	0.15560653381609132	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3029	0.13952995587002387	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2305	0.10617911795325354	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	4.606469325520761E-5	0.0
6	0.0	0.0	0.0	4.606469325520761E-5	4.606469325520761E-5
7	0.0	0.0	0.0	4.606469325520761E-5	4.606469325520761E-5
8	0.0	0.0	0.0	4.606469325520761E-5	4.606469325520761E-5
9	0.0	0.0	0.0	4.606469325520761E-5	4.606469325520761E-5
10	0.0	0.0	0.0	9.212938651041522E-5	9.212938651041522E-5
11	0.0	0.0	0.0	9.212938651041522E-5	9.212938651041522E-5
12	0.0	0.0	0.0	9.212938651041522E-5	9.212938651041522E-5
13	0.0	0.0	0.0	9.212938651041522E-5	1.3819407976562283E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	50	0.0014968127	13.303616	15
TAGGACC	845	0.0	12.250989	4
CGGTACA	60	0.0058892523	11.0802145	4
TGTAGGA	2750	0.0	10.775148	2
ACTGTTC	605	0.0	10.671239	8
CTGTAGG	2745	0.0	10.453214	1
AGGACCT	1480	0.0	10.395722	5
GGACCTG	1420	0.0	10.363218	6
AATGTCC	715	0.0	10.357379	8
GTCCTAC	2495	0.0	10.201555	1
GGCGAGG	1110	0.0	10.1783	19
GTAGGAC	2760	0.0	10.151129	3
AGGACGT	2555	0.0	10.036314	5
GGACGTG	2505	0.0	10.0057	6
TGGCGAG	2375	0.0	9.922467	18
AATGCCG	230	0.0	9.91551	18
TTAGGAC	1570	0.0	9.860282	3
TATACAC	925	0.0	9.856685	3
GGTATCA	1045	0.0	9.852199	1
TAGGACA	880	0.0	9.821099	4
>>END_MODULE