FastQCFastQC Report
Thu 26 May 2016
SRR1512969_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512969_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2170860
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT44260.2038823323475489No Hit
CTGTAGGACGTGGAATATGGCAAGA43800.20176335645780935No Hit
GTATCAACGCAGAGTACTTTTTTTT42500.19577494633463235No Hit
CTTTAGGACGTGAAATATGGCGAGG42390.19526823470882507No Hit
GTCCTAAAGTGTGTATTTCTCATTT40850.1881742719475231No Hit
GTCCTACAGTGTGCATTTCTCATTT29510.13593690979611767No Hit
TATCAACGCAGAGTACTTTTTTTTT28960.13340335166708125No Hit
GGTATCAACGCAGAGTACTTTTTTT25910.11935362022424292No Hit
CTGTAGGACCTGGAATATGGCGAGA23120.10650157080604002No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG500.00147991313.3236745
AGAACCG655.364167E-513.1772595
TATGCCG550.003028508712.1124315
TAGGACC9200.012.1030264
TCCAACG1650.012.0767218
CCGTACT657.906813E-411.71311954
GACCGCT700.001486641210.8616657
CCAACGA1408.511051E-910.84440219
GGCGAGG12000.010.83310719
GGACCTG15300.010.81965356
AGGACCT15950.010.6804195
TTTAGAA7700.010.4964652
GCCGGTT1106.1110713E-610.35457511
GTCCTAC25750.010.3398861
GGTATCA11250.010.3119841
CCTACAC3050.010.2881913
TTAGGAC16550.010.2844233
CCGGTTT1201.5428377E-610.28195812
TGGCGAG25600.010.23016918
AGGACGT23550.010.2241125
Produced by FastQC (version 0.11.5)