##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512969_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2170860 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.300749933206195 31.0 31.0 34.0 26.0 34.0 2 30.426421786757324 31.0 31.0 34.0 27.0 34.0 3 30.535790424071568 31.0 31.0 34.0 27.0 34.0 4 34.009977151912146 37.0 35.0 37.0 30.0 37.0 5 33.644866089936706 37.0 35.0 37.0 28.0 37.0 6 33.50136351492036 37.0 35.0 37.0 28.0 37.0 7 33.369590853394506 37.0 35.0 37.0 27.0 37.0 8 33.23505845609574 37.0 35.0 37.0 27.0 37.0 9 34.66806058428457 39.0 35.0 39.0 27.0 39.0 10 34.53436886763771 39.0 35.0 39.0 27.0 39.0 11 34.573108353371474 39.0 35.0 39.0 27.0 39.0 12 34.42637249753554 39.0 35.0 39.0 27.0 39.0 13 34.40808619625402 39.0 35.0 39.0 27.0 39.0 14 35.43114710299144 40.0 36.0 41.0 26.0 41.0 15 35.445803967091386 40.0 36.0 41.0 26.0 41.0 16 35.4231498115954 40.0 36.0 41.0 26.0 41.0 17 35.39356245911758 40.0 36.0 41.0 26.0 41.0 18 35.31263876988843 40.0 36.0 41.0 25.0 41.0 19 35.29760739983232 40.0 35.0 41.0 25.0 41.0 20 35.27656320536561 40.0 35.0 41.0 25.0 41.0 21 35.21788922362566 40.0 35.0 41.0 25.0 41.0 22 35.173996020010506 40.0 35.0 41.0 25.0 41.0 23 35.06135264365275 40.0 35.0 41.0 24.0 41.0 24 35.05334844255272 40.0 35.0 41.0 24.0 41.0 25 34.951587849976505 40.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7983406931364563 1101 2 0.7924342853953483 1101 3 0.7561526441833344 1101 4 0.5732927899569091 1101 5 0.8348654205692227 1101 6 0.833737308575671 1101 7 1.0024897484223132 1101 8 0.903923220017532 1101 9 0.7754263133138153 1101 10 1.1765488150878554 1101 11 1.2049829609041325 1101 12 1.1542426969631023 1101 13 1.2746458306223687 1101 14 1.3800864932024908 1101 15 1.3077869749747109 1101 16 1.3968598845404472 1101 17 1.3330339151725639 1101 18 0.9238512134109271 1101 19 1.2413415930529226 1101 20 1.3289844350034414 1101 21 1.4226484058892979 1101 22 1.2029572781430886 1101 23 1.223833131852949 1101 24 1.2221468509192306 1101 25 1.2622057104012256 1102 1 0.5125967846991628 1102 2 0.45677947339783387 1102 3 0.451904373124453 1102 4 0.4290270853089382 1102 5 0.6269654627757006 1102 6 0.4386698094361492 1102 7 0.5386897916940541 1102 8 0.556323864906048 1102 9 0.7508538054152538 1102 10 0.7026995660435631 1102 11 0.7081730579202556 1102 12 0.7206785932593291 1102 13 0.8030780822479073 1102 14 1.023751976936829 1102 15 0.8490086339133143 1102 16 0.8714867759801734 1102 17 0.9481565802709682 1102 18 0.8304626798343762 1102 19 1.0178974196588726 1102 20 0.9296621262907934 1102 21 0.9235586552307637 1102 22 0.826926589736722 1102 23 0.8392988852826377 1102 24 0.8492428947140525 1102 25 0.9341295139357726 1103 1 0.3891587543256847 1103 2 0.3100887874821012 1103 3 0.42255137421130584 1103 4 0.45709104557762004 1103 5 0.4490703743249611 1103 6 0.5018209795629147 1103 7 0.5365449410490299 1103 8 0.5601289367239204 1103 9 0.6199899601446219 1103 10 0.6760334019823304 1103 11 0.6841060733616615 1103 12 0.6216527580174755 1103 13 0.7125925112923426 1103 14 0.7533150240387769 1103 15 0.528878492773778 1103 16 0.6998254978930376 1103 17 0.8198935377501897 1103 18 0.8277514962743311 1103 19 0.8576114972883246 1103 20 0.9585772091792109 1103 21 0.5285136714433918 1103 22 0.7750773684805523 1103 23 0.8665719634390996 1103 24 0.8881710681894006 1103 25 1.0003875792198897 1104 1 0.4926004769075334 1104 2 0.4739705598212183 1104 3 0.3805622660946888 1104 4 0.49255663654493986 1104 5 0.4891873509296403 1104 6 0.5297821925459729 1104 7 0.654621385142633 1104 8 0.49956973027541807 1104 9 0.6866241986175936 1104 10 0.7347657172769857 1104 11 0.7403118749566886 1104 12 0.6746427538425408 1104 13 0.8185726810541141 1104 14 0.8889262034656014 1104 15 0.8789528365748467 1104 16 0.9462572091754851 1104 17 0.832419595484005 1104 18 0.933652940688539 1104 19 1.0420765638949518 1104 20 0.9758661504408366 1104 21 0.7980628494937676 1104 22 0.915913274933466 1104 23 0.8134302164915823 1104 24 0.8109796181183668 1104 25 0.9839359408064752 1105 1 0.5665168834076226 1105 2 0.6477504795612603 1105 3 0.5996820052953531 1105 4 0.541115732825368 1105 5 0.4939425853737589 1105 6 0.598043522137857 1105 7 0.5654956534131941 1105 8 0.7060436051615042 1105 9 0.8517641597134329 1105 10 0.8320189256089492 1105 11 0.7550045153188023 1105 12 0.6736832837908224 1105 13 0.8146849798526006 1105 14 0.9669696095475047 1105 15 0.91984308959195 1105 16 0.8918821027914632 1105 17 0.6628836539705318 1105 18 0.956253722694278 1105 19 0.8620598261421648 1105 20 1.0054578949706823 1105 21 0.8888270702794046 1105 22 0.9767627465332183 1105 23 0.8521398159552973 1105 24 0.9165948732543967 1105 25 1.0035332785966773 1106 1 0.5992244808461678 1106 2 0.5825243619327338 1106 3 0.5279690769504413 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0.457409341680318 1107 17 0.4828408787772034 1107 18 0.5806811883550296 1107 19 0.7057071257734009 1107 20 0.7071442637960885 1107 21 0.7196360898789109 1107 22 0.7313975727281488 1107 23 0.6871816034572333 1107 24 0.7598962887404213 1107 25 0.730931479128003 1108 1 0.6237798002142618 1108 2 0.5107564170384222 1108 3 0.6222910697454616 1108 4 0.6388429643140583 1108 5 0.7086381847421777 1108 6 0.649530655569059 1108 7 0.7017525550753874 1108 8 0.7650748940534413 1108 9 0.6448442762279285 1108 10 0.8590552794929636 1108 11 0.7755832925814161 1108 12 0.800066011536984 1108 13 0.8013819989382327 1108 14 0.9124526042283136 1108 15 0.9280874376438035 1108 16 0.7420861659434408 1108 17 0.7920465482061729 1108 18 0.8794153440683132 1108 19 0.9096535903073288 1108 20 0.9186370660979932 1108 21 1.1292747153243283 1108 22 1.1111586858570988 1108 23 1.0174588648397318 1108 24 1.0251900128765996 1108 25 1.0215888018850734 1109 1 0.07445963121324439 1109 2 0.1643452089250843 1109 3 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2216 18 -1.721645291034875 2216 19 -2.011377687330892 2216 20 -2.338690530255363 2216 21 -2.1254734258739845 2216 22 -2.4717334447953903 2216 23 -1.5575112900856283 2216 24 -1.7574115696689958 2216 25 -1.7613013110722306 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 61712.0 3 0.0 4 1053.0 5 9013.0 6 10303.0 7 7971.0 8 6410.0 9 5574.0 10 4861.0 11 4335.0 12 3981.0 13 3669.0 14 3816.0 15 3743.0 16 3867.0 17 3975.0 18 4344.0 19 5053.0 20 6481.0 21 8438.0 22 10591.0 23 12979.0 24 15227.0 25 17663.0 26 20178.0 27 21974.0 28 25209.0 29 30322.0 30 37997.0 31 48621.0 32 61830.0 33 79879.0 34 103445.0 35 135830.0 36 186366.0 37 290879.0 38 913271.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.072104591072325 19.807026521175914 13.56512278663661 27.555746101115155 2 13.160867121785834 20.92009618307952 40.87665717733985 25.04237951779479 3 16.628340841878334 25.449361082704552 30.512285453691163 27.410012621725954 4 11.132880808296774 17.846984116159252 38.51735767279684 32.50277740274713 5 12.235338697320127 38.61351359574274 36.58385585645389 12.567291850483247 6 30.920786040574388 38.43811615428907 18.238097806519544 12.402999998617002 7 26.890199844430274 31.931700465603235 23.187861506077173 17.990238183889318 8 24.158563983616094 38.14001362559842 20.793373054687695 16.90804933609779 9 26.02538778963823 14.89338953352794 20.4758182778444 38.60540439898943 10 14.259290088343516 29.41944701026117 35.312550294747396 21.008712606647922 11 33.48071541269823 22.36814041068282 25.748684225679767 18.402459950939182 12 22.886871032239636 24.8856547898757 32.62906450691507 19.598409670969584 13 28.721935507350192 22.332787748432693 26.02988171418384 22.915395030033274 14 20.162340952854787 22.68272570644112 28.06522199802832 29.089711342675766 15 22.222288763944007 32.03629185506291 23.485501454576447 22.25591792641663 16 21.438200528540836 28.33761190949199 28.574930062326537 21.649257499640637 17 21.06467210554107 29.18967037767094 28.366420894419303 21.379236622368683 18 20.931739850251656 27.734123441503343 30.650917938753718 20.683218769491283 19 23.0699423208831 27.095273039515792 27.876366487424235 21.95841815217687 20 23.229087873726144 27.804460528825203 28.394204094944936 20.572247502503714 21 22.25548607969752 27.03783481001521 27.563617703230705 23.143061407056564 22 22.70052794817899 28.641131129318975 27.646454833275524 21.011886089226515 23 21.515344149323308 28.41201182941323 28.207530656053358 21.8651133652101 24 22.74504113577108 27.160848695908534 28.791170319596844 21.302939848723547 25 22.149775612057155 27.725661670338138 28.46207424564468 21.662488471960028 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 158.0 1 158.0 2 334.0 3 510.0 4 510.0 5 510.0 6 1244.5 7 1979.0 8 1979.0 9 1979.0 10 2990.0 11 4001.0 12 4001.0 13 4001.0 14 7065.5 15 10130.0 16 10130.0 17 10130.0 18 18699.0 19 27268.0 20 27268.0 21 27268.0 22 44328.0 23 61388.0 24 61388.0 25 61388.0 26 88345.5 27 115303.0 28 115303.0 29 115303.0 30 148616.0 31 181929.0 32 181929.0 33 181929.0 34 203779.5 35 225630.0 36 225630.0 37 225630.0 38 240719.5 39 255809.0 40 255809.0 41 255809.0 42 265391.0 43 274973.0 44 274973.0 45 274973.0 46 277974.0 47 280975.0 48 280975.0 49 280975.0 50 266116.5 51 251258.0 52 251258.0 53 251258.0 54 220620.5 55 189983.0 56 189983.0 57 189983.0 58 160869.0 59 131755.0 60 131755.0 61 131755.0 62 106586.5 63 81418.0 64 81418.0 65 81418.0 66 62864.5 67 44311.0 68 44311.0 69 44311.0 70 32189.0 71 20067.0 72 20067.0 73 20067.0 74 13942.5 75 7818.0 76 7818.0 77 7818.0 78 5403.0 79 2988.0 80 2988.0 81 2988.0 82 1936.5 83 885.0 84 885.0 85 885.0 86 576.5 87 268.0 88 268.0 89 268.0 90 159.0 91 50.0 92 50.0 93 50.0 94 28.0 95 6.0 96 6.0 97 6.0 98 3.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07600674387109256 2 0.0 3 0.0 4 0.030725150401223474 5 0.07269008595671762 6 0.07642132611038943 7 0.03556194319302028 8 0.06513547626286356 9 0.0459725638686972 10 0.11023281095971181 11 0.003915498926692647 12 0.011055526381249826 13 0.0046525340187759685 14 0.005527763190624913 15 0.005758086656900952 16 0.016399030798853912 17 0.02570409883640585 18 8.291644785937371E-4 19 0.002625687515546834 20 0.003961563619947855 21 9.212938651041522E-5 22 1.3819407976562283E-4 23 0.0 24 0.0 25 0.0030402697548437027 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2170860.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.90388627628523 #Duplication Level Percentage of deduplicated Percentage of total 1 83.04288437836428 58.88063230018245 2 11.669321459329389 16.548004833474117 3 2.878432673914941 6.122761888956258 4 0.9711022682768825 2.7541969917018685 5 0.48659707860347556 1.7250811961836727 6 0.257170645791863 1.094063893369505 7 0.14864569888047077 0.7377690410215887 8 0.09501360056941746 0.5389466823579492 9 0.07196236319956731 0.45921700948273686 >10 0.3380004386514315 4.192680477464869 >50 0.020986551112247755 1.0513153466986365 >100 0.016170076682761478 2.3466335997369496 >500 0.002084360209545453 1.0853734099265104 >1k 0.0016284064137067596 2.463323329442783 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 4426 0.2038823323475489 No Hit CTGTAGGACGTGGAATATGGCAAGA 4380 0.20176335645780935 No Hit GTATCAACGCAGAGTACTTTTTTTT 4250 0.19577494633463235 No Hit CTTTAGGACGTGAAATATGGCGAGG 4239 0.19526823470882507 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4085 0.1881742719475231 No Hit GTCCTACAGTGTGCATTTCTCATTT 2951 0.13593690979611767 No Hit TATCAACGCAGAGTACTTTTTTTTT 2896 0.13340335166708125 No Hit GGTATCAACGCAGAGTACTTTTTTT 2591 0.11935362022424292 No Hit CTGTAGGACCTGGAATATGGCGAGA 2312 0.10650157080604002 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.606469325520761E-5 2 0.0 0.0 0.0 0.0 4.606469325520761E-5 3 0.0 0.0 0.0 0.0 4.606469325520761E-5 4 0.0 0.0 0.0 0.0 4.606469325520761E-5 5 0.0 0.0 0.0 0.0 4.606469325520761E-5 6 0.0 0.0 0.0 0.0 4.606469325520761E-5 7 0.0 0.0 0.0 4.606469325520761E-5 4.606469325520761E-5 8 0.0 0.0 0.0 4.606469325520761E-5 4.606469325520761E-5 9 0.0 0.0 0.0 4.606469325520761E-5 4.606469325520761E-5 10 0.0 0.0 0.0 4.606469325520761E-5 4.606469325520761E-5 11 0.0 0.0 0.0 4.606469325520761E-5 4.606469325520761E-5 12 0.0 0.0 0.0 4.606469325520761E-5 9.212938651041522E-5 13 0.0 0.0 0.0 4.606469325520761E-5 1.3819407976562283E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACACCG 50 0.001479913 13.323674 5 AGAACCG 65 5.364167E-5 13.177259 5 TATGCCG 55 0.0030285087 12.112431 5 TAGGACC 920 0.0 12.103026 4 TCCAACG 165 0.0 12.07672 18 CCGTACT 65 7.906813E-4 11.7131195 4 GACCGCT 70 0.0014866412 10.861665 7 CCAACGA 140 8.511051E-9 10.844402 19 GGCGAGG 1200 0.0 10.833107 19 GGACCTG 1530 0.0 10.8196535 6 AGGACCT 1595 0.0 10.680419 5 TTTAGAA 770 0.0 10.496465 2 GCCGGTT 110 6.1110713E-6 10.354575 11 GTCCTAC 2575 0.0 10.339886 1 GGTATCA 1125 0.0 10.311984 1 CCTACAC 305 0.0 10.288191 3 TTAGGAC 1655 0.0 10.284423 3 CCGGTTT 120 1.5428377E-6 10.281958 12 TGGCGAG 2560 0.0 10.230169 18 AGGACGT 2355 0.0 10.224112 5 >>END_MODULE