FastQCFastQC Report
Thu 26 May 2016
SRR1512968_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512968_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227603
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT39810.17871227503284923No Hit
GTCCTAAAGTGTGTATTTCTCATTT37970.17045227538300137No Hit
CTGTAGGACGTGGAATATGGCAAGA37390.1678485798412015No Hit
CTTTAGGACGTGAAATATGGCGAGG35580.15972325409868815No Hit
GTATCAACGCAGAGTACTTTTTTTT34790.15617684120554695No Hit
GTCCTACAGTGTGCATTTCTCATTT23840.10702086502846332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7150.011.8202424
TCCAACG1451.209628E-911.14254218
ATATCGA600.00590317911.076726
GTCCTAA11850.010.8489641
GTATTAT2900.010.83649351
CGTATCC803.7905565E-410.6818428
CCAACGA909.5718104E-510.54973119
GGACGTG21350.010.49490456
AGGACGT21550.010.4872675
TAGGACA6450.010.4530024
TGTCCAC7300.010.40568110
TTTAGAA7950.010.3921232
GGACCTG13750.010.3574526
TTAGGAC14250.010.3290223
GGCGAGG10200.010.23944519
ATTTAGA6800.010.2231991
AGGACCT14600.010.2112625
AATGTCC6850.010.1187288
GACGTGA11850.010.0952397
ATTATAC2550.010.0546263
Produced by FastQC (version 0.11.5)