##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512968_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2227603 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.99660397297005 31.0 30.0 34.0 22.0 34.0 2 29.02713005863253 31.0 30.0 34.0 20.0 34.0 3 29.173058215489924 31.0 30.0 34.0 22.0 34.0 4 32.568089107439704 35.0 33.0 37.0 25.0 37.0 5 32.67701605716997 35.0 33.0 37.0 25.0 37.0 6 32.68835111103729 37.0 33.0 37.0 25.0 37.0 7 32.508735622999254 36.0 33.0 37.0 23.0 37.0 8 32.5144709357996 37.0 33.0 37.0 23.0 37.0 9 33.826554821483 38.0 34.0 39.0 22.0 39.0 10 33.66822364667313 38.0 34.0 39.0 22.0 39.0 11 33.76582048057935 38.0 34.0 39.0 22.0 39.0 12 33.66129108283658 38.0 34.0 39.0 21.0 39.0 13 33.67265127583326 38.0 34.0 39.0 21.0 39.0 14 34.68577300353788 39.0 34.0 41.0 19.0 41.0 15 34.63416775789941 39.0 34.0 41.0 18.0 41.0 16 34.543881023683305 39.0 34.0 41.0 17.0 41.0 17 34.504628966651595 39.0 34.0 41.0 17.0 41.0 18 34.2078404455372 39.0 34.0 41.0 17.0 41.0 19 34.43686734126323 39.0 34.0 41.0 18.0 41.0 20 34.50691393394604 39.0 34.0 41.0 18.0 41.0 21 34.42986833829906 39.0 34.0 41.0 18.0 41.0 22 34.366247486648206 39.0 34.0 41.0 16.0 41.0 23 34.107538910658675 39.0 34.0 41.0 10.0 41.0 24 34.11679863961397 39.0 34.0 41.0 2.0 41.0 25 34.037772888616146 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9414240200175072 1101 2 1.1790326691217494 1101 3 1.2523469762263062 1101 4 1.25739117664574 1101 5 1.032525801403871 1101 6 1.2258165555827532 1101 7 1.218560354323614 1101 8 1.0595804812267176 1101 9 1.0956264965735727 1101 10 1.1419098193815245 1101 11 1.30814466431125 1101 12 1.4573491891133017 1101 13 1.3700066488248126 1101 14 1.4789292309007749 1101 15 1.2355185528189452 1101 16 1.6870469874259584 1101 17 1.5711090718463367 1101 18 1.8447854015049714 1101 19 1.2890142410972487 1101 20 1.4792131521453555 1101 21 1.5414167693443872 1101 22 1.585128225980057 1101 23 1.841468135742879 1101 24 1.3956194034151537 1101 25 1.6689267998162407 1102 1 0.8272868327871343 1102 2 0.8321505046942868 1102 3 0.9175614468290441 1102 4 1.0023505011865979 1102 5 0.9230139069135461 1102 6 0.7971717879259046 1102 7 0.9899359243963346 1102 8 0.883841543719015 1102 9 0.9911657545545864 1102 10 1.1416294052313916 1102 11 1.1516766500132078 1102 12 1.1502858340042224 1102 13 1.0810309308413295 1102 14 1.1630972328729214 1102 15 1.3953207221767627 1102 16 1.3152153591749993 1102 17 1.202216553665636 1102 18 1.4412410899063062 1102 19 1.4521049596970315 1102 20 1.4383552697344086 1102 21 1.16671443974991 1102 22 1.5195150126187897 1102 23 1.6347819530730874 1102 24 1.5353044767542414 1102 25 1.3932994116737518 1103 1 0.5931533433257776 1103 2 0.8341477764777672 1103 3 0.7256839268502908 1103 4 0.9592561483533544 1103 5 0.40266455033805926 1103 6 0.8216587383592824 1103 7 0.7040296977853586 1103 8 0.741664040640714 1103 9 1.0466318449961705 1103 10 1.0536220897481599 1103 11 1.0245387422896997 1103 12 1.1250214625804773 1103 13 1.1579207640096811 1103 14 1.2591757234207392 1103 15 1.3558503100470674 1103 16 1.3604180439332012 1103 17 1.2408866084713992 1103 18 1.53178950971364 1103 19 1.5720589663123548 1103 20 1.4360568076759321 1103 21 1.3148203814674773 1103 22 1.521021567581485 1103 23 1.7195830105587788 1103 24 1.5231971105696402 1103 25 1.552728055532846 1104 1 0.781417908948832 1104 2 0.7372491100790022 1104 3 0.7783576487712409 1104 4 1.039061462665579 1104 5 0.7417297255829638 1104 6 0.7813882133192536 1104 7 0.9380719053346667 1104 8 0.921910326158347 1104 9 1.0366573029072583 1104 10 1.0559532734448425 1104 11 1.0474311970436574 1104 12 0.9641693600049734 1104 13 1.1670881770284893 1104 14 1.2529388558339392 1104 15 1.4389320211777914 1104 16 1.314960603105213 1104 17 1.26593539745717 1104 18 1.4997946226710965 1104 19 1.3598100986657045 1104 20 1.4475652370019034 1104 21 1.4375172527735671 1104 22 1.4327871410471147 1104 23 1.7162514656205445 1104 24 1.579260945586988 1104 25 1.2701734578255568 1105 1 0.6742778413177426 1105 2 0.8153397017635946 1105 3 0.8588735335835551 1105 4 0.8109231994738906 1105 5 0.6325052681430066 1105 6 0.5455623054997147 1105 7 0.8115573045304529 1105 8 0.7917518735629798 1105 9 0.7321225559992399 1105 10 0.9001729470802928 1105 11 0.7650187773045758 1105 12 0.8454211763102109 1105 13 0.818035153822251 1105 14 0.9559959941945522 1105 15 1.1527332763748177 1105 16 1.0874824133401404 1105 17 1.0805060582883428 1105 18 1.0805489066533838 1105 19 1.1189209355442102 1105 20 1.1794178808345492 1105 21 1.1230926457824708 1105 22 0.895863497894787 1105 23 1.091370602753777 1105 24 1.241371132134546 1105 25 1.1496961931791603 1106 1 0.6553881382338389 1106 2 0.7446189119565574 1106 3 0.7479443855913033 1106 4 0.72938403006642 1106 5 0.743661065306199 1106 6 0.7453788275994242 1106 7 0.5451530737459294 1106 8 0.7679315569357925 1106 9 0.8741242260457653 1106 10 0.9772109434788945 1106 11 0.9244031322633077 1106 12 0.9915053716488487 1106 13 0.9097252522641028 1106 14 1.1682674278783693 1106 15 1.1875918052569645 1106 16 1.1244682633881382 1106 17 1.0556024835935247 1106 18 1.3364789919166427 1106 19 0.913571674284178 1106 20 1.1084783944590555 1106 21 0.9584226503836177 1106 22 0.9840175284441415 1106 23 1.311793453988983 1106 24 1.2019500815957542 1106 25 1.3443202338964895 1107 1 0.7833104370911173 1107 2 0.8192729605720217 1107 3 0.9893478116481944 1107 4 1.0433176767447847 1107 5 0.9666720777476456 1107 6 0.8603758059423257 1107 7 1.0194117956416875 1107 8 1.0723627806178087 1107 9 1.1740823440961563 1107 10 0.9804262134595021 1107 11 0.8141312803358431 1107 12 1.1411317554948965 1107 13 1.1154890834336726 1107 14 1.303579258030453 1107 15 1.3846590156598921 1107 16 1.279007472831914 1107 17 1.3626015487430436 1107 18 1.5853486653346494 1107 19 1.512166968276027 1107 20 1.3894686878919345 1107 21 1.4550838408030984 1107 22 1.4333763376682143 1107 23 1.4737967235353793 1107 24 1.6281144727034942 1107 25 1.4705811074065167 1108 1 0.571765172672901 1108 2 0.6606803403359827 1108 3 0.4925843626788655 1108 4 0.7717110827458313 1108 5 0.7944361201872496 1108 6 0.7780489748662518 1108 7 0.9484065762131806 1108 8 0.9001919363350765 1108 9 1.0424628181467313 1108 10 0.85597141567996 1108 11 0.7912745761950291 1108 12 0.9944156675265319 1108 13 0.9856188694215575 1108 14 1.0678944607173975 1108 15 1.1627367680402543 1108 16 1.0283890586674076 1108 17 1.1122685315816483 1108 18 1.3241404350163535 1108 19 1.3011775963178849 1108 20 1.1292138325794667 1108 21 1.0849922805205168 1108 22 0.9680609820784483 1108 23 1.1334897905586487 1108 24 1.2920586916810706 1108 25 1.3628610698805375 1109 1 0.4340519608372979 1109 2 0.4202605371789865 1109 3 0.5791775607521394 1109 4 0.6801003689814138 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17 0.30808490139471445 1110 18 0.7792760887157755 1110 19 0.6070584595448167 1110 20 0.5422699445085897 1110 21 0.5734889634234648 1110 22 0.7406557022748714 1110 23 0.6428404705731552 1110 24 0.7549106647034662 1110 25 0.8432605952223398 1111 1 0.3110477316720761 1111 2 0.6269953626458253 1111 3 0.4762475155235677 1111 4 0.3862548189496451 1111 5 0.3856456229740175 1111 6 0.6131956928813835 1111 7 0.4869074387205856 1111 8 0.42354157167009276 1111 9 0.7987022334243861 1111 10 0.7110888503889399 1111 11 0.4949806708978173 1111 12 0.766378802544331 1111 13 0.717122149046979 1111 14 0.6512516069451735 1111 15 0.8161129995382908 1111 16 0.6615753419158921 1111 17 0.6564700699383224 1111 18 0.9489408654835785 1111 19 0.572121255446568 1111 20 0.485186892986448 1111 21 0.6346487194397881 1111 22 0.46954906951415154 1111 23 0.8465517311740953 1111 24 0.9934345089658194 1111 25 0.4623640139407712 1112 1 -0.24406734107266814 1112 2 -0.06413607835773405 1112 3 -0.0062760767726608435 1112 4 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1.5706007231051586 2206 25 1.1057212986260794 2207 1 0.9839275762052715 2207 2 0.9961511409770623 2207 3 0.9306074923815615 2207 4 1.056163783839395 2207 5 0.9531029198757253 2207 6 0.8061376285354029 2207 7 0.845839362655255 2207 8 0.8420555879123484 2207 9 1.0320459235193127 2207 10 1.141096976463416 2207 11 1.0691688463880595 2207 12 1.2029374841752798 2207 13 0.8371230885728309 2207 14 1.0360842806874047 2207 15 1.2045898797850043 2207 16 1.2643605415816168 2207 17 1.4571891368737653 2207 18 1.6339420769774406 2207 19 1.3310707336800576 2207 20 1.2912919979084094 2207 21 1.299815468795714 2207 22 1.1107449705441752 2207 23 1.3239229213555532 2207 24 1.2993950587697611 2207 25 0.9766935138987378 2208 1 0.6553600127370558 2208 2 0.5726999786058329 2208 3 0.6044134536590846 2208 4 0.7863149269436747 2208 5 0.5854078624643009 2208 6 0.6537630789516911 2208 7 0.7596242532640503 2208 8 0.778385274188949 2208 9 0.9770227826778779 2208 10 0.8874971305990513 2208 11 0.7685723906877087 2208 12 0.8762597219294932 2208 13 0.6597572501118947 2208 14 0.8480146245000455 2208 15 0.9171830887986587 2208 16 1.0045386994148657 2208 17 0.8754513396195165 2208 18 1.0494167204363052 2208 19 0.9979102563515241 2208 20 0.833022170368146 2208 21 1.052438849039298 2208 22 1.0181537292194278 2208 23 0.991422849616896 2208 24 1.0127388591149256 2208 25 0.8966471092353174 2209 1 0.5309408299303442 2209 2 0.6216150049382065 2209 3 0.478400193516503 2209 4 0.592879811678209 2209 5 0.3618662037658069 2209 6 0.5656346813935258 2209 7 0.4864679768131879 2209 8 0.6215332859158735 2209 9 0.625733269564904 2209 10 0.7488511484688161 2209 11 0.3624405309252694 2209 12 0.44070744048321586 2209 13 0.31514121056095235 2209 14 0.5988496609429887 2209 15 0.5910053656386438 2209 16 0.7274078723271913 2209 17 0.5923569405081821 2209 18 0.9251721444731871 2209 19 0.6488159669930624 2209 20 0.637585395506207 2209 21 0.5732626274981385 2209 22 0.5322408430680738 2209 23 0.8115744748740283 2209 24 0.7760818983715296 2209 25 0.6000422977358753 2210 1 0.42819528929775785 2210 2 0.4039718363711451 2210 3 0.2883003888390441 2210 4 0.42172541769934213 2210 5 0.43233042676327926 2210 6 0.2552184953858969 2210 7 0.33911352145047857 2210 8 0.2386100726258391 2210 9 0.3318051139515177 2210 10 0.5170054680282874 2210 11 0.34872968967319196 2210 12 0.4671949893402001 2210 13 0.3445765943404737 2210 14 0.3992167155579125 2210 15 0.30411389619283113 2210 16 0.30888365270534024 2210 17 0.4798529743111217 2210 18 0.5238632872843283 2210 19 0.3611100186135374 2210 20 0.17442169894083293 2210 21 0.4352996079384823 2210 22 0.33182973671108584 2210 23 0.3119164960367016 2210 24 0.295228273863259 2210 25 0.2295266134835856 2211 1 -0.09788210366821914 2211 2 0.2783593104227542 2211 3 0.20135126587963015 2211 4 0.234054163993207 2211 5 0.022884946243515003 2211 6 -0.033786424232935985 2211 7 0.10756888601553527 2211 8 0.012884120289832879 2211 9 -0.08844369617946768 2211 10 0.08886355951770497 2211 11 -0.04125690524303849 2211 12 0.029001290004565305 2211 13 -0.05053659183295878 2211 14 0.22585163189462776 2211 15 0.08457859497961095 2211 16 -0.03272801138367498 2211 17 0.026345175923452757 2211 18 0.1091840492469558 2211 19 -0.09104748489302494 2211 20 -0.15477752402694023 2211 21 -0.02389253154168358 2211 22 -0.1850201155127067 2211 23 0.3455836057650785 2211 24 0.04908112334538117 2211 25 0.08922980257356272 2212 1 0.01268604605033019 2212 2 0.05497736958257704 2212 3 -0.017773486220129087 2212 4 -0.12847348500839928 2212 5 -0.06435834444462074 2212 6 -0.17072803169188688 2212 7 -0.1362007419654958 2212 8 -0.11567701735845759 2212 9 0.010727151436533688 2212 10 0.05437646987873279 2212 11 -0.2097953470073719 2212 12 -0.05102173820499445 2212 13 -0.09631884232241106 2212 14 -0.12858633220052695 2212 15 -0.32800315467559926 2212 16 -0.11442928389751472 2212 17 -0.22378946481790507 2212 18 -2.83849861498787 2212 19 -1.1151704140891 2212 20 -0.46971077056464594 2212 21 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-2.2426356091083903 2214 8 -1.9710631339211346 2214 9 -2.177093592855705 2214 10 -2.1851716317003564 2214 11 -2.0096171536756735 2214 12 -2.4735801709467253 2214 13 -2.335041511290342 2214 14 -3.5579346191520536 2214 15 -3.9255980880704513 2214 16 -3.0477357943757966 2214 17 -3.432722298841078 2214 18 -2.7704004245673346 2214 19 -4.878777743957034 2214 20 -3.24863824824973 2214 21 -3.1308763871721617 2214 22 -3.2591043513900466 2214 23 -3.416978183721774 2214 24 -3.622942684084652 2214 25 -3.4307507035143914 2215 1 -1.1715363006477553 2215 2 -2.783682131520095 2215 3 -1.6057786447314584 2215 4 -1.1727743479927994 2215 5 -1.0229344177715198 2215 6 -1.5803802663954656 2215 7 -1.9730254302571915 2215 8 -2.0620665177712034 2215 9 -1.8260531501134807 2215 10 -3.3208147670205435 2215 11 -2.296431994727655 2215 12 -2.497739555766799 2215 13 -1.7958039561189167 2215 14 -3.2248269199195825 2215 15 -3.0638436634634516 2215 16 -2.6076212261600134 2215 17 -2.9000492160302187 2215 18 -1.8257642035391797 2215 19 -2.4596004328424996 2215 20 -2.486897606421998 2215 21 -2.2847724035024797 2215 22 -1.8449800370047669 2215 23 -2.3970135757823137 2215 24 -2.388270073046673 2215 25 -2.378318679611233 2216 1 -3.1812046020995837 2216 2 -3.246720900194802 2216 3 -3.783186520436697 2216 4 -4.412270055085276 2216 5 -3.489902178658646 2216 6 -3.710508526786093 2216 7 -3.4892262470111852 2216 8 -4.1371758759583805 2216 9 -4.403086015042547 2216 10 -3.981377331078896 2216 11 -4.5274407880837835 2216 12 -4.832295146444693 2216 13 -5.525950096972263 2216 14 -4.990993891511998 2216 15 -4.90993215259628 2216 16 -4.02171198866596 2216 17 -4.239263066364362 2216 18 -4.125804274933312 2216 19 -4.632674531395779 2216 20 -4.342922524123971 2216 21 -4.779286533857441 2216 22 -5.319791094793786 2216 23 -5.377263806472211 2216 24 -5.252656342128478 2216 25 -5.182924175937753 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 116686.0 3 1.0 4 917.0 5 5669.0 6 6813.0 7 5965.0 8 5393.0 9 5118.0 10 4692.0 11 4410.0 12 4247.0 13 4004.0 14 4229.0 15 4188.0 16 4441.0 17 4549.0 18 5285.0 19 6509.0 20 8151.0 21 10263.0 22 13209.0 23 15697.0 24 18265.0 25 20240.0 26 22632.0 27 25300.0 28 28792.0 29 33791.0 30 41508.0 31 52371.0 32 65346.0 33 83568.0 34 108232.0 35 143119.0 36 195158.0 37 303922.0 38 844923.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.0276642552484 18.832039769704075 12.613247827628573 25.527048147418945 2 14.479959689443447 20.338822443135886 41.835271018434355 23.34594684898631 3 18.298961201794143 25.229342142370857 29.991952592145516 26.479744063689488 4 12.026222275911405 17.267309410012263 39.10443149816621 31.60203681591012 5 12.815218133283516 38.24612253403224 36.26373774426149 12.67492158842275 6 31.00887303239317 37.86237236204414 18.640192744425956 12.488561861136729 7 27.27689475574713 31.958961925287355 22.995644755747126 17.76849856321839 8 24.524453013762045 37.402556815758125 21.073097486514776 16.999892683965054 9 26.337573468993725 15.179221688900062 20.452914737801564 38.03029010430464 10 14.621808517564464 29.074476267534266 35.275032227445166 21.028682987456104 11 33.66191042569995 22.5271520609704 25.44622592077242 18.36471159255722 12 23.037586719846907 24.87316710242674 32.36226861050233 19.72697756722402 13 28.72488549261859 22.372593840825196 25.966830239018584 22.93569042753763 14 20.518900967051284 22.489645151092784 28.064738910522607 28.926714971333322 15 22.603026375937716 31.397146056901043 23.52859556147096 22.471232005690283 16 21.788623069873616 28.28440843582365 28.25957817289676 21.66739032140598 17 21.434835182450353 28.812684124055554 28.1507916801184 21.601689013375697 18 21.400252921313072 27.663246147084443 30.098310352606823 20.838190578995665 19 23.23933258465613 27.1891960580843 27.76956308353828 21.801908273721295 20 23.343002110367742 27.712675676889226 28.14211306182928 20.802209150913743 21 22.65071288217058 27.121987448675718 27.42095252122122 22.806347147932478 22 22.90489900087466 28.44970338747133 27.3776596795543 21.26773793209971 23 21.943982707541384 28.249363072482662 27.885750874007343 21.920903345968608 24 22.82720214012176 27.20456473100532 28.51636813291169 21.451864995961227 25 22.400880790232637 27.607807673777142 28.13158510361723 21.85972643237299 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 265.0 1 265.0 2 490.5 3 716.0 4 716.0 5 716.0 6 1326.5 7 1937.0 8 1937.0 9 1937.0 10 3051.5 11 4166.0 12 4166.0 13 4166.0 14 7391.0 15 10616.0 16 10616.0 17 10616.0 18 19079.0 19 27542.0 20 27542.0 21 27542.0 22 44047.0 23 60552.0 24 60552.0 25 60552.0 26 86418.0 27 112284.0 28 112284.0 29 112284.0 30 145365.0 31 178446.0 32 178446.0 33 178446.0 34 201681.0 35 224916.0 36 224916.0 37 224916.0 38 241457.0 39 257998.0 40 257998.0 41 257998.0 42 269147.5 43 280297.0 44 280297.0 45 280297.0 46 285216.5 47 290136.0 48 290136.0 49 290136.0 50 276584.0 51 263032.0 52 263032.0 53 263032.0 54 232416.0 55 201800.0 56 201800.0 57 201800.0 58 171913.0 59 142026.0 60 142026.0 61 142026.0 62 115390.0 63 88754.0 64 88754.0 65 88754.0 66 68128.5 67 47503.0 68 47503.0 69 47503.0 70 34565.5 71 21628.0 72 21628.0 73 21628.0 74 15076.0 75 8524.0 76 8524.0 77 8524.0 78 5769.0 79 3014.0 80 3014.0 81 3014.0 82 2018.0 83 1022.0 84 1022.0 85 1022.0 86 670.5 87 319.0 88 319.0 89 319.0 90 199.0 91 79.0 92 79.0 93 79.0 94 47.0 95 15.0 96 15.0 97 15.0 98 15.5 99 16.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3602078108172776 2 0.039998150478339274 3 0.03586815065341535 4 0.03564369414119122 5 0.039998150478339274 6 0.018180977490154217 7 0.018091194885264564 8 0.024061738110426317 9 0.02123358605640233 10 0.020829564334398903 11 0.019033912236605895 12 0.020649999124619605 13 0.02038065130995065 14 0.028146846632905415 15 0.02913445528669157 16 0.021637607778405756 17 0.02262521643219191 18 0.10796358237980465 19 0.025318694578881425 20 0.022580325129747086 21 0.026351194535112407 22 0.020425542612395476 23 0.02267010773463674 24 0.019752173075723096 25 0.022984346851750513 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2227603.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.3851667731237 #Duplication Level Percentage of deduplicated Percentage of total 1 82.39304388162026 58.81641178434761 2 12.01069640051232 17.14771131223857 3 3.04418592209878 6.519291592122502 4 1.1243974401531127 3.2106119513841334 5 0.48475124310080675 1.7302024166115062 6 0.25872205275375176 1.1081350130226888 7 0.1613907139297545 0.8064632120656317 8 0.10400829003646386 0.5939719304032315 9 0.06863691813499896 0.44096920630741265 >10 0.3146348647358678 3.8213313326980023 >50 0.01836098448524218 0.9056414650284401 >100 0.01395467981730942 2.050508047581194 >500 0.0021444057475941336 1.1009964835359385 >1k 0.0010722028737969306 1.7477542526530399 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 3981 0.17871227503284923 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3797 0.17045227538300137 No Hit CTGTAGGACGTGGAATATGGCAAGA 3739 0.1678485798412015 No Hit CTTTAGGACGTGAAATATGGCGAGG 3558 0.15972325409868815 No Hit GTATCAACGCAGAGTACTTTTTTTT 3479 0.15617684120554695 No Hit GTCCTACAGTGTGCATTTCTCATTT 2384 0.10702086502846332 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 715 0.0 11.820242 4 TCCAACG 145 1.209628E-9 11.142542 18 ATATCGA 60 0.005903179 11.07672 6 GTCCTAA 1185 0.0 10.848964 1 GTATTAT 290 0.0 10.8364935 1 CGTATCC 80 3.7905565E-4 10.681842 8 CCAACGA 90 9.5718104E-5 10.549731 19 GGACGTG 2135 0.0 10.4949045 6 AGGACGT 2155 0.0 10.487267 5 TAGGACA 645 0.0 10.453002 4 TGTCCAC 730 0.0 10.405681 10 TTTAGAA 795 0.0 10.392123 2 GGACCTG 1375 0.0 10.357452 6 TTAGGAC 1425 0.0 10.329022 3 GGCGAGG 1020 0.0 10.239445 19 ATTTAGA 680 0.0 10.223199 1 AGGACCT 1460 0.0 10.211262 5 AATGTCC 685 0.0 10.118728 8 GACGTGA 1185 0.0 10.095239 7 ATTATAC 255 0.0 10.054626 3 >>END_MODULE