FastQCFastQC Report
Thu 26 May 2016
SRR1512968_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512968_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227603
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT39040.17525564474459768No Hit
CTGTAGGACGTGGAATATGGCAAGA38070.1709011884074496No Hit
GTATCAACGCAGAGTACTTTTTTTT37830.16982379714877383No Hit
CTTTAGGACGTGAAATATGGCGAGG37040.16627738425563263No Hit
GTCCTAAAGTGTGTATTTCTCATTT36920.16573868862629473No Hit
TATCAACGCAGAGTACTTTTTTTTT27070.12152075571814187No Hit
GGTATCAACGCAGAGTACTTTTTTT24380.1094449953604839No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG500.001484441913.3182994
CCGTGCG655.447476E-513.1556679
GGCGAGG9350.012.38546919
TAGGACC6600.011.6751334
TAATACC1657.8216544E-1110.9544454
TTAGACT2000.010.9400324
GTATTAG1701.364242E-1010.6243681
TGGCGAG22400.010.50892318
TGGACCG750.002618304210.1472755
AGGACGT22600.010.1444695
GTTCTAG2350.010.1127811
GAACCGG856.5313646E-410.0694926
GGACGTG22550.010.037246
TGTAGGA21000.09.958682
AGGACCT14350.09.9439765
CTAGTAC1151.0188125E-59.9195413
GTAGGAC19950.09.8159533
CTTAGAC2054.0017767E-119.7380863
AGGACTG4600.09.7198765
ATGGCGA24950.09.550282517
Produced by FastQC (version 0.11.5)