##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512968_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2227603 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.36375826392764 31.0 31.0 34.0 26.0 34.0 2 30.511651313093044 31.0 31.0 34.0 27.0 34.0 3 30.601155591907535 31.0 31.0 34.0 27.0 34.0 4 34.07227230345802 37.0 35.0 37.0 30.0 37.0 5 33.69582596180738 37.0 35.0 37.0 28.0 37.0 6 33.54501497798306 37.0 35.0 37.0 28.0 37.0 7 33.421431018004554 37.0 35.0 37.0 27.0 37.0 8 33.29659414177481 37.0 35.0 37.0 27.0 37.0 9 34.75147142466589 39.0 35.0 39.0 27.0 39.0 10 34.634183469855266 39.0 35.0 39.0 27.0 39.0 11 34.64893744531678 39.0 35.0 39.0 27.0 39.0 12 34.507291469799604 39.0 35.0 39.0 27.0 39.0 13 34.485852281578 39.0 35.0 39.0 27.0 39.0 14 35.534210090397615 40.0 36.0 41.0 26.0 41.0 15 35.54902466911743 40.0 36.0 41.0 27.0 41.0 16 35.5266355809361 40.0 36.0 41.0 26.0 41.0 17 35.50061972443025 40.0 36.0 41.0 26.0 41.0 18 35.432215255590876 40.0 36.0 41.0 26.0 41.0 19 35.410747785848734 40.0 36.0 41.0 25.0 41.0 20 35.38451420652603 40.0 36.0 41.0 25.0 41.0 21 35.325668891629256 40.0 36.0 41.0 25.0 41.0 22 35.28458841184897 40.0 36.0 41.0 25.0 41.0 23 35.17660417947004 40.0 36.0 41.0 25.0 41.0 24 35.16497418974566 40.0 36.0 41.0 25.0 41.0 25 35.071454383927474 40.0 36.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7158933985604925 1101 2 0.7541767077049535 1101 3 0.7543454145229163 1101 4 0.6271805561287565 1101 5 0.8429556413537611 1101 6 0.8706162801410855 1101 7 0.864601088536304 1101 8 0.9603974422733472 1101 9 0.8735574612601482 1101 10 1.1540638804980858 1101 11 1.0760816610404689 1101 12 1.1776497009279439 1101 13 1.2394242137240497 1101 14 1.4026418873080218 1101 15 1.320211609401035 1101 16 1.4367297617723125 1101 17 1.433778167554074 1101 18 1.02286103517352 1101 19 1.2265564681790977 1101 20 1.2263341366113494 1101 21 1.510544573771682 1101 22 1.3699441986338101 1101 23 1.2220236319786224 1101 24 1.2344856975506318 1101 25 1.2819073277619069 1102 1 0.6697076472970878 1102 2 0.532207645705693 1102 3 0.5894390913379048 1102 4 0.4976612661004083 1102 5 0.7002667469866921 1102 6 0.5365444201632741 1102 7 0.5606376964495325 1102 8 0.5969344815929603 1102 9 0.834681583040009 1102 10 0.8361833184371932 1102 11 0.7695920764231872 1102 12 0.7664140297828652 1102 13 1.0232551024212029 1102 14 1.0827404944816905 1102 15 0.9928136061963002 1102 16 0.9461658519978826 1102 17 0.8911780196433128 1102 18 0.9864669762557341 1102 19 1.0070168404211728 1102 20 0.9682723838688361 1102 21 1.0040851876013406 1102 22 1.0880269054007243 1102 23 1.0488343062051726 1102 24 0.9580990341708713 1102 25 0.9880511709765045 1103 1 0.625019822868687 1103 2 0.46398835021328466 1103 3 0.5583513055391158 1103 4 0.6167720606632869 1103 5 0.6578134043178707 1103 6 0.731386763049386 1103 7 0.7304130410981244 1103 8 0.7781635069194976 1103 9 0.8865176969007962 1103 10 0.9182465020002937 1103 11 1.0383636546843746 1103 12 0.9697464376150222 1103 13 0.9391560174601992 1103 14 1.1695653816107878 1103 15 0.9575761547851087 1103 16 1.0701349241628293 1103 17 1.1831557351844921 1103 18 1.2152430210660867 1103 19 1.2211305322051373 1103 20 1.2629855589817112 1103 21 0.8700856946150282 1103 22 1.1132478185082348 1103 23 1.1910244846257 1103 24 1.2123633814511052 1103 25 1.2351388602012534 1104 1 0.5626614071159892 1104 2 0.6065393996307016 1104 3 0.5165349231275194 1104 4 0.443931868371628 1104 5 0.5794393760897307 1104 6 0.7100021177394353 1104 7 0.7488714160241088 1104 8 0.6312989340288624 1104 9 0.77171213677331 1104 10 0.8272880148542896 1104 11 0.9205091302941568 1104 12 0.8631575198220602 1104 13 0.8566779431128353 1104 14 1.0870898722922533 1104 15 1.060424405542328 1104 16 0.9943464449897945 1104 17 0.9208460549794566 1104 18 1.0641482881390303 1104 19 0.9516631390653103 1104 20 0.9996776550591377 1104 21 0.819427230340537 1104 22 0.8967610957386896 1104 23 0.8416004404730089 1104 24 0.893815444268661 1104 25 1.0441985420094255 1105 1 0.43526049099036257 1105 2 0.5047688507130736 1105 3 0.47043262048963896 1105 4 0.36620099880170187 1105 5 0.3641882409249746 1105 6 0.43892560767679356 1105 7 0.46101320316021344 1105 8 0.46605616739941524 1105 9 0.6570342254598032 1105 10 0.6249531518692848 1105 11 0.5356281175431761 1105 12 0.5283058593964043 1105 13 0.672630120056148 1105 14 0.82287198061357 1105 15 0.7529313605862242 1105 16 0.7828698106897889 1105 17 0.5727955906298376 1105 18 0.7626242987541616 1105 19 0.745670518364804 1105 20 0.7968713229506932 1105 21 0.785810449303888 1105 22 0.8319310935820212 1105 23 0.7975093341859463 1105 24 0.8009681687889696 1105 25 0.8362413556025956 1106 1 0.5038573678897578 1106 2 0.4852747498399914 1106 3 0.49261981339137506 1106 4 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1107 17 0.824302846950161 1107 18 0.9924562431246713 1107 19 1.1488019444126323 1107 20 1.0776803716146688 1107 21 0.9733830272938064 1107 22 0.8998212107517318 1107 23 0.9985028024132987 1107 24 1.026632731802927 1107 25 1.0525582678268464 1108 1 0.4855046155169127 1108 2 0.45416063244893934 1108 3 0.4524288017239506 1108 4 0.4326868841660456 1108 5 0.5354786060052064 1108 6 0.5336521220081991 1108 7 0.5226814988380752 1108 8 0.5592849324921048 1108 9 0.5390955995924003 1108 10 0.6965913530255534 1108 11 0.6391710870942902 1108 12 0.7779871962499598 1108 13 0.6579777808999125 1108 14 0.7714598031383204 1108 15 0.6857776455320845 1108 16 0.6486141390049909 1108 17 0.6960594930397335 1108 18 0.7813204472784463 1108 19 0.7265692263187873 1108 20 0.73641034523245 1108 21 0.8606677323468546 1108 22 0.814473253170604 1108 23 0.9535924578895489 1108 24 0.9822951249653187 1108 25 0.8767265023953215 1109 1 0.24821391760002953 1109 2 0.30388929562636946 1109 3 0.3250712209745288 1109 4 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-2.9344959770435537 2216 22 -3.08645142525738 2216 23 -2.353224741764727 2216 24 -2.689971512891205 2216 25 -2.470592883440254 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 63254.0 3 2.0 4 1034.0 5 9475.0 6 10623.0 7 8285.0 8 6555.0 9 5839.0 10 4995.0 11 4589.0 12 4126.0 13 3893.0 14 3697.0 15 3639.0 16 3830.0 17 3956.0 18 4516.0 19 5177.0 20 6581.0 21 8376.0 22 10768.0 23 12963.0 24 15351.0 25 17570.0 26 19809.0 27 21790.0 28 24822.0 29 29611.0 30 37021.0 31 48011.0 32 60366.0 33 78764.0 34 102477.0 35 133931.0 36 185493.0 37 289379.0 38 977035.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.24507449745386 19.5480510262072 13.500290891610867 27.70658358472808 2 13.343278262130426 20.748733727449395 40.588642220936336 25.319345789483844 3 16.612295817522245 25.011862526671045 30.542919900897964 27.832921754908753 4 11.14115169958334 17.638989360961695 38.40278367139099 32.81707526806398 5 12.38229791133682 38.348475898053294 36.47934808732 12.789878103289887 6 31.088708137625538 38.127180302754624 18.219592568382605 12.564518991237234 7 27.019936781761462 31.904014973355206 23.124682011541662 17.95136623334167 8 24.407267977355733 37.59090923383128 20.827495812481835 17.174326976331145 9 25.92943885843266 14.87367140418549 20.313756768445405 38.883132968936444 10 14.23034631079492 29.188526317634228 35.37841555408065 21.202711817490208 11 33.547830427880854 22.434115542913784 25.421631794054058 18.596422235151305 12 22.7215627469291 24.93337403922132 32.399342719632216 19.94572049421737 13 28.53468659750402 22.15988036828682 26.03911195630264 23.266321077906515 14 20.347353100417784 22.320350316097965 27.994490620317002 29.337805963167245 15 22.370397572847352 31.443981636532275 23.564130720738273 22.6214900698821 16 21.671055437698534 28.263460955652086 28.199930408234273 21.865553198415103 17 21.326964421382733 28.84298997605821 28.151138627109724 21.678906975449337 18 21.19863062894821 27.69138154563313 30.045537962068458 21.0644498633502 19 23.145642905594528 27.091994751186395 27.845557744805845 21.916804598413233 20 23.243371369415687 27.661844892154285 28.164598161015725 20.930185577414306 21 22.432393607469923 26.993760100556653 27.513646974322143 23.060199317651282 22 22.79693589788481 28.298187916872042 27.4339850655548 21.470891119688346 23 21.724347527071714 28.02601182796568 28.048098358683465 22.201542286279146 24 22.65741366725295 27.166477674198532 28.56910704874569 21.60700160980283 25 22.344734766402382 27.515401609048958 28.076876284776798 22.062987339771865 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 164.0 1 164.0 2 311.0 3 458.0 4 458.0 5 458.0 6 1111.0 7 1764.0 8 1764.0 9 1764.0 10 2829.0 11 3894.0 12 3894.0 13 3894.0 14 7195.0 15 10496.0 16 10496.0 17 10496.0 18 18929.0 19 27362.0 20 27362.0 21 27362.0 22 44304.0 23 61246.0 24 61246.0 25 61246.0 26 87074.5 27 112903.0 28 112903.0 29 112903.0 30 145106.5 31 177310.0 32 177310.0 33 177310.0 34 200734.5 35 224159.0 36 224159.0 37 224159.0 38 240802.5 39 257446.0 40 257446.0 41 257446.0 42 268807.5 43 280169.0 44 280169.0 45 280169.0 46 285668.0 47 291167.0 48 291167.0 49 291167.0 50 278119.5 51 265072.0 52 265072.0 53 265072.0 54 233325.5 55 201579.0 56 201579.0 57 201579.0 58 172327.0 59 143075.0 60 143075.0 61 143075.0 62 115477.5 63 87880.0 64 87880.0 65 87880.0 66 67680.5 67 47481.0 68 47481.0 69 47481.0 70 34423.0 71 21365.0 72 21365.0 73 21365.0 74 14875.5 75 8386.0 76 8386.0 77 8386.0 78 5677.5 79 2969.0 80 2969.0 81 2969.0 82 1958.0 83 947.0 84 947.0 85 947.0 86 604.0 87 261.0 88 261.0 89 261.0 90 151.5 91 42.0 92 42.0 93 42.0 94 24.0 95 6.0 96 6.0 97 6.0 98 4.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07577651852686498 2 8.978260488965044E-5 3 0.0 4 0.027653042306012336 5 0.0690428231601412 6 0.07667434457576149 7 0.032052389945605206 8 0.0603339104858451 9 0.04085108522479095 10 0.1001973870568499 11 0.0030077172638032896 12 0.01090858649409253 13 0.004489130244482522 14 0.005790978015382454 15 0.006150108434941056 16 0.014724347201902672 17 0.024016846807981496 18 4.938043268930774E-4 19 0.0023343477271309115 20 0.0030974998686929406 21 1.3467390733447568E-4 22 4.489130244482522E-5 23 4.489130244482522E-5 24 4.489130244482522E-5 25 0.0034117389858067167 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2227603.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.30092601121547 #Duplication Level Percentage of deduplicated Percentage of total 1 81.45243247802789 57.26181429071363 2 12.594223087256232 17.70771090851884 3 3.259842171454768 6.875097699110448 4 1.1904352122037438 3.347547910971239 5 0.5128364776638417 1.8026439636049045 6 0.28177391312089634 1.1885380210921674 7 0.16338262674192633 0.8040164967871539 8 0.10529406612824284 0.5921816281841302 9 0.06615281466269633 0.41855437161322784 >10 0.3373524348025548 4.0210131318643505 >50 0.018559894314692592 0.9184451191010584 >100 0.01451996302326258 2.1227601098832665 >500 0.0020447107835782374 1.0787168459739636 >1k 0.0011501498157622217 1.8609595025816181 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 3904 0.17525564474459768 No Hit CTGTAGGACGTGGAATATGGCAAGA 3807 0.1709011884074496 No Hit GTATCAACGCAGAGTACTTTTTTTT 3783 0.16982379714877383 No Hit CTTTAGGACGTGAAATATGGCGAGG 3704 0.16627738425563263 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3692 0.16573868862629473 No Hit TATCAACGCAGAGTACTTTTTTTTT 2707 0.12152075571814187 No Hit GGTATCAACGCAGAGTACTTTTTTT 2438 0.1094449953604839 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.978260488965044E-5 0.0 5 0.0 0.0 0.0 8.978260488965044E-5 0.0 6 0.0 0.0 0.0 8.978260488965044E-5 0.0 7 0.0 0.0 0.0 8.978260488965044E-5 0.0 8 0.0 0.0 0.0 8.978260488965044E-5 0.0 9 0.0 0.0 0.0 8.978260488965044E-5 0.0 10 0.0 0.0 0.0 8.978260488965044E-5 0.0 11 0.0 0.0 0.0 1.3467390733447568E-4 0.0 12 0.0 0.0 0.0 1.3467390733447568E-4 0.0 13 0.0 0.0 0.0 1.7956520977930088E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTACG 50 0.0014844419 13.318299 4 CCGTGCG 65 5.447476E-5 13.155667 9 GGCGAGG 935 0.0 12.385469 19 TAGGACC 660 0.0 11.675133 4 TAATACC 165 7.8216544E-11 10.954445 4 TTAGACT 200 0.0 10.940032 4 GTATTAG 170 1.364242E-10 10.624368 1 TGGCGAG 2240 0.0 10.508923 18 TGGACCG 75 0.0026183042 10.147275 5 AGGACGT 2260 0.0 10.144469 5 GTTCTAG 235 0.0 10.112781 1 GAACCGG 85 6.5313646E-4 10.069492 6 GGACGTG 2255 0.0 10.03724 6 TGTAGGA 2100 0.0 9.95868 2 AGGACCT 1435 0.0 9.943976 5 CTAGTAC 115 1.0188125E-5 9.919541 3 GTAGGAC 1995 0.0 9.815953 3 CTTAGAC 205 4.0017767E-11 9.738086 3 AGGACTG 460 0.0 9.719876 5 ATGGCGA 2495 0.0 9.5502825 17 >>END_MODULE