FastQCFastQC Report
Thu 26 May 2016
SRR1512967_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512967_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2501616
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT45260.18092305133961406No Hit
GTCCTAAAGTGTGTATTTCTCATTT40770.16297465318418175No Hit
GTATCAACGCAGAGTACTTTTTTTT39240.1568586065966959No Hit
CTGTAGGACGTGGAATATGGCAAGA39060.1561390717040505No Hit
CTTTAGGACGTGAAATATGGCGAGG38000.1519018106695832No Hit
GTCCTACAGTGTGCATTTCTCATTT32060.12815715921228518No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACT500.001503709413.2956334
TCCAACG1800.012.66985418
TGAACCG853.9605384E-612.2900815
TAGGACC9600.012.2668044
GGCGAGG11800.011.34524519
GTCCTAA13200.011.2636771
AATAGCG600.00589173911.0796945
AGGACCG951.3659639E-510.9963885
GTCCTAC27600.010.9466111
TGTAGGA25100.010.8213632
CAGCGTA803.7908653E-410.6818559
TTATACT3750.010.6365064
TCCTACA29000.010.5450242
AATCCCG3450.010.45782319
GTCCTAT3200.010.4243161
AGGACGT23850.010.3131745
GACGTGA14100.010.3026537
CTGTAGG25750.010.2895661
GGACGTG24050.010.2644356
GTATTAG2700.010.2367891
Produced by FastQC (version 0.11.5)