##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512967_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2501616 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.00810795901529 31.0 30.0 34.0 22.0 34.0 2 29.04725465459127 31.0 30.0 34.0 20.0 34.0 3 29.189736554291308 31.0 30.0 34.0 22.0 34.0 4 32.59167154351427 35.0 33.0 37.0 25.0 37.0 5 32.69619358046958 35.0 33.0 37.0 25.0 37.0 6 32.706829105666095 37.0 35.0 37.0 25.0 37.0 7 32.52376943543693 36.0 33.0 37.0 23.0 37.0 8 32.53555101982079 37.0 33.0 37.0 24.0 37.0 9 33.85397399121208 38.0 34.0 39.0 23.0 39.0 10 33.69056441915946 38.0 34.0 39.0 22.0 39.0 11 33.787232732761545 38.0 34.0 39.0 22.0 39.0 12 33.68194039372949 38.0 34.0 39.0 21.0 39.0 13 33.7062374880877 38.0 34.0 39.0 22.0 39.0 14 34.712642547857065 39.0 34.0 41.0 19.0 41.0 15 34.67196764011743 39.0 34.0 41.0 19.0 41.0 16 34.57367317765796 39.0 34.0 41.0 18.0 41.0 17 34.533803349514876 39.0 34.0 41.0 17.0 41.0 18 34.23582995951417 39.0 34.0 41.0 17.0 41.0 19 34.46919831021228 39.0 34.0 41.0 18.0 41.0 20 34.54306376358322 39.0 34.0 41.0 18.0 41.0 21 34.47346395290084 39.0 34.0 41.0 18.0 41.0 22 34.40272527838005 39.0 34.0 41.0 17.0 41.0 23 34.14942141399799 39.0 34.0 41.0 10.0 41.0 24 34.15476995669999 39.0 34.0 41.0 2.0 41.0 25 34.076953457285214 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7897666474156146 1101 2 1.0652914175115207 1101 3 1.0792887696928837 1101 4 1.0527186385062564 1101 5 0.849040403970001 1101 6 1.0500901996088245 1101 7 0.9876349461469829 1101 8 0.8591185865273445 1101 9 0.8555822663395105 1101 10 0.9791540416718476 1101 11 1.0872248083807676 1101 12 1.2451970040320788 1101 13 1.1366474990454094 1101 14 1.1078153032168743 1101 15 0.9551718781402698 1101 16 1.460580229041632 1101 17 1.393385824004156 1101 18 1.5656631481286567 1101 19 0.9832337026298319 1101 20 1.2836880416133596 1101 21 1.2438814665897553 1101 22 1.1835330398517385 1101 23 1.4369834291096026 1101 24 1.1716082385405642 1101 25 1.3662409087738325 1102 1 0.9755978142127972 1102 2 0.9372559176420339 1102 3 0.9429671826398938 1102 4 1.061454947563945 1102 5 0.9584228106523298 1102 6 0.9100214969202298 1102 7 1.056280626126103 1102 8 1.0775795627737565 1102 9 1.0934366124632433 1102 10 1.1886189333450403 1102 11 1.253073933935454 1102 12 1.2862795412286303 1102 13 1.1871677809561376 1102 14 1.3934359766702826 1102 15 1.4766549373937252 1102 16 1.4453311556926423 1102 17 1.305784675478968 1102 18 1.6624836127593028 1102 19 1.604259648857969 1102 20 1.5381843089064944 1102 21 1.2647027665114479 1102 22 1.5614606827648103 1102 23 1.7566413774259644 1102 24 1.614725753549699 1102 25 1.5130965600398198 1103 1 0.5437546218217868 1103 2 0.6842189308781599 1103 3 0.5808513956899013 1103 4 0.7284030280233011 1103 5 0.2180224356993321 1103 6 0.5277898402434076 1103 7 0.3965649487764864 1103 8 0.41762818421337755 1103 9 0.7349389145996597 1103 10 0.6668514735238986 1103 11 0.7032605696218965 1103 12 0.7647430466300307 1103 13 0.7692695342807525 1103 14 0.9864110784820141 1103 15 0.9686228729689077 1103 16 1.094926026219305 1103 17 0.944406947678452 1103 18 1.2433955348409 1103 19 1.159381095039329 1103 20 1.0928240570397776 1103 21 0.8457475021756977 1103 22 1.0577894339265868 1103 23 1.3904389817434861 1103 24 1.1294612317915096 1103 25 1.128056668261955 1104 1 0.8178953926363306 1104 2 0.8092719527913239 1104 3 0.6948723637144312 1104 4 0.9982937514711523 1104 5 0.7018377623294043 1104 6 0.8234463344715266 1104 7 1.0273767605369741 1104 8 1.0431498691133712 1104 9 1.074730684830996 1104 10 1.000554806357286 1104 11 1.0838379470668968 1104 12 1.00688709787984 1104 13 0.9610424938316484 1104 14 1.2178492274434944 1104 15 1.210829577132273 1104 16 1.1353538126356568 1104 17 1.1341922195550822 1104 18 1.4644905377706436 1104 19 1.2725652285248614 1104 20 1.2917271100874643 1104 21 1.3017990195963591 1104 22 1.2171595952914274 1104 23 1.45619204919997 1104 24 1.3366115058328845 1104 25 0.9552287433237083 1105 1 0.8083015086328302 1105 2 0.8583222298364497 1105 3 0.9141204478186786 1105 4 0.9562606807777101 1105 5 0.6491290212350833 1105 6 0.6605780020025094 1105 7 0.9092970800914699 1105 8 0.9792886909327407 1105 9 0.8909120346104586 1105 10 1.0244354269532323 1105 11 0.9643972046119842 1105 12 0.9590096854682884 1105 13 1.0115123831808859 1105 14 1.2030680661402258 1105 15 1.376182114962269 1105 16 1.3611948457974279 1105 17 1.250477751684322 1105 18 1.2865152998984541 1105 19 1.2970806086649702 1105 20 1.2634533586492651 1105 21 1.265207873516161 1105 22 0.8830832308372223 1105 23 1.1893244527918014 1105 24 1.2765928069133707 1105 25 1.2718859304188541 1106 1 0.6843988400645387 1106 2 0.7362977546471328 1106 3 0.7235067671580282 1106 4 0.7283931632210852 1106 5 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1.3738126430781534 1107 19 1.1788054198015487 1107 20 1.1622819810072968 1107 21 1.146412779721068 1107 22 1.1439694034881 1107 23 1.2478763584025288 1107 24 1.2252728850052108 1107 25 1.325493434026356 1108 1 0.5332934117747214 1108 2 0.6195093727077463 1108 3 0.5090182627028206 1108 4 0.6239932890031383 1108 5 0.5734278050142976 1108 6 0.6195214948757908 1108 7 0.7477837081479919 1108 8 0.8064738164414393 1108 9 0.813547568680761 1108 10 0.7547848316331098 1108 11 0.7096664455712656 1108 12 0.7449366705303078 1108 13 0.7139030826735038 1108 14 0.9631708026273529 1108 15 0.885716462947407 1108 16 0.9501678322082157 1108 17 0.8832460227577386 1108 18 1.1058043642310693 1108 19 1.155132102495081 1108 20 1.0070045987971241 1108 21 0.9890714278496517 1108 22 0.7399928006736971 1108 23 0.8943624420791139 1108 24 0.9975719239035428 1108 25 1.1829510317315552 1109 1 0.14980289140448022 1109 2 0.06868701356315299 1109 3 0.31664437586853467 1109 4 0.3571086157272134 1109 5 0.28561949787027885 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0.14590201732752917 1110 18 0.6373833275008565 1110 19 0.49641197866370845 1110 20 0.4452597406666712 1110 21 0.4519536140735596 1110 22 0.4888532192239339 1110 23 0.4558031600513033 1110 24 0.6548919455659785 1110 25 0.686214999456439 1111 1 0.13140713282744088 1111 2 0.3491974335197803 1111 3 0.3465792421948173 1111 4 0.2226722661850573 1111 5 0.20025444992920427 1111 6 0.42149593159953014 1111 7 0.20212314650846253 1111 8 0.09312116850513519 1111 9 0.3937335702382967 1111 10 0.38999318445522135 1111 11 0.33451218250935 1111 12 0.3753848687364396 1111 13 0.448438255564902 1111 14 0.3341469324448596 1111 15 0.5900215553930437 1111 16 0.396631455481085 1111 17 0.4254539882179458 1111 18 0.6994544210437112 1111 19 0.4358655642358187 1111 20 0.483073944014599 1111 21 0.7025882055517982 1111 22 0.4510017721006392 1111 23 0.7142973459699178 1111 24 0.7783434825374655 1111 25 0.3697864806819098 1112 1 -0.11992346339001614 1112 2 0.1289128454878572 1112 3 0.1645321255205907 1112 4 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1.2980115127630327 2209 24 1.1550779781905902 2209 25 1.1197244768489512 2210 1 0.35177196459549265 2210 2 0.4759742376360343 2210 3 0.3492965098914347 2210 4 0.5152403278887263 2210 5 0.2839103349812717 2210 6 0.259016735028645 2210 7 0.297489363108113 2210 8 0.26826750121689713 2210 9 0.36536856955337527 2210 10 0.5074865336792556 2210 11 0.3381425267048499 2210 12 0.5004555603167873 2210 13 0.4267652173694927 2210 14 0.41250963268010565 2210 15 0.2882401601527178 2210 16 0.3635757531876891 2210 17 0.429007563866179 2210 18 0.6334736142232202 2210 19 0.5267859826230961 2210 20 0.40776100139797933 2210 21 0.4462000262397652 2210 22 0.6714654645530658 2210 23 0.5015886453303651 2210 24 0.5196293726479198 2210 25 0.4749590645873951 2211 1 -0.25504395359319076 2211 2 0.17596526550673275 2211 3 -0.013023447114846931 2211 4 0.08769389711957842 2211 5 -0.157847857977508 2211 6 -0.14696531890019315 2211 7 0.04291742883485483 2211 8 -0.16370880511153985 2211 9 -0.1560326776632266 2211 10 0.004204909415712166 2211 11 -0.06561643296316788 2211 12 0.007081795004125979 2211 13 -0.15242333963117005 2211 14 0.21377696860115947 2211 15 0.09948060687413118 2211 16 -0.056590874660166435 2211 17 0.13407146989626995 2211 18 0.1648638320647109 2211 19 -0.0023485150328212967 2211 20 -0.08776426239621316 2211 21 -0.11008649100620715 2211 22 -0.12370218285494161 2211 23 0.4010319201756829 2211 24 0.22505941405565721 2211 25 0.2657149619498611 2212 1 -0.03891849615492049 2212 2 -0.09174229035365045 2212 3 -0.046314226561801064 2212 4 -0.17209434526402845 2212 5 -0.05692214284648145 2212 6 -0.19933202261339744 2212 7 -0.17869014748597323 2212 8 -0.12689389786716276 2212 9 -0.11667740906622726 2212 10 -0.09093334908970263 2212 11 -0.22247806302996764 2212 12 -0.031143570250065977 2212 13 -0.2866643611293682 2212 14 -0.2403135357344368 2212 15 -0.23784485094837038 2212 16 -0.10986071977234957 2212 17 -0.13720094253766035 2212 18 -2.6355563275267713 2212 19 -0.9967932636623047 2212 20 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-1.7107022682153108 2214 7 -1.904616310655296 2214 8 -1.7143885396711518 2214 9 -1.7859708130420202 2214 10 -1.8709090143875784 2214 11 -1.6777526469185347 2214 12 -2.1678787150661343 2214 13 -1.9845691892157973 2214 14 -3.15232310915248 2214 15 -3.4720245285281273 2214 16 -2.6869334964485603 2214 17 -3.0851664779738996 2214 18 -2.404686130613676 2214 19 -4.482842413807937 2214 20 -2.979901973070927 2214 21 -2.8305843881221726 2214 22 -2.8551079206734435 2214 23 -2.8438593453570142 2214 24 -3.0765256130617473 2214 25 -2.9581895999935526 2215 1 -1.3507615342485089 2215 2 -2.927232561475094 2215 3 -1.8613667124287936 2215 4 -1.4064400125698313 2215 5 -1.3252988605614355 2215 6 -1.8520293055597925 2215 7 -2.1913629186216994 2215 8 -2.227781260956135 2215 9 -1.9457022617065824 2215 10 -3.6682514828155277 2215 11 -2.469320183993407 2215 12 -2.6663991322160143 2215 13 -2.0347391811782813 2215 14 -3.426936637132222 2215 15 -3.059070860378629 2215 16 -2.894400825016998 2215 17 -3.152301347661659 2215 18 -2.0901166857977955 2215 19 -2.7658023251620705 2215 20 -2.9052427836704915 2215 21 -2.5243655423917737 2215 22 -2.10214450632688 2215 23 -2.6745194348117707 2215 24 -2.581062969729217 2215 25 -2.3904132176454205 2216 1 -2.272201681230083 2216 2 -2.2435530382968842 2216 3 -2.7737466006301403 2216 4 -3.5111816086598573 2216 5 -2.538678678958494 2216 6 -2.7132557136273476 2216 7 -2.405494790493261 2216 8 -2.9459839447945697 2216 9 -3.3583902896990097 2216 10 -2.853984967959107 2216 11 -3.4357595945852246 2216 12 -3.7582079563361113 2216 13 -4.408757972700947 2216 14 -3.869620101198027 2216 15 -3.6558680264024765 2216 16 -2.7354843409745584 2216 17 -2.9372883428755827 2216 18 -2.976233587574061 2216 19 -3.192998531240452 2216 20 -3.131607824746478 2216 21 -3.4742847994973474 2216 22 -3.9818334989703885 2216 23 -3.9578505604923784 2216 24 -3.928751635319486 2216 25 -4.061614541665101 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 131337.0 3 2.0 4 1074.0 5 6298.0 6 7639.0 7 6795.0 8 5874.0 9 5602.0 10 5102.0 11 4872.0 12 4667.0 13 4561.0 14 4572.0 15 4563.0 16 4882.0 17 5177.0 18 5823.0 19 7085.0 20 8874.0 21 11376.0 22 14266.0 23 17275.0 24 19803.0 25 22495.0 26 25351.0 27 28002.0 28 31665.0 29 37733.0 30 46319.0 31 58207.0 32 72764.0 33 93944.0 34 122212.0 35 160579.0 36 221297.0 37 343832.0 38 949697.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.73906247743331 19.050618387458616 12.931775984389066 25.278543150719006 2 14.285040167990138 20.30669957046704 42.27482511358169 23.13343514796113 3 18.326587281593078 25.203041361654755 29.95894785585923 26.511423500892946 4 12.012038070437747 17.29484915022915 39.07426342596351 31.618849353369594 5 12.789715120712883 38.32367632926938 36.2432981164943 12.643310433523434 6 31.069440079834383 37.87055539429821 18.563013108223405 12.496991417644006 7 27.27369045600949 31.915387001954283 22.969035365763947 17.84188717627228 8 24.321681095354087 37.724127121720755 21.046838190253958 16.9073535926712 9 26.306909209547275 15.316593305934841 20.58785075912265 37.78864672539524 10 14.584762690694264 29.266561753835475 35.176304733391866 20.972370822078393 11 33.73325619545257 22.608848973012 25.39872674518791 18.259168086347515 12 22.935728096647747 24.93674843790232 32.47280022901703 19.6547232364329 13 28.7041572026073 22.429230959298778 26.022088424867327 22.844523413226586 14 20.43240731744084 22.62411287965392 28.161975010086255 28.78150479281899 15 22.50602581142529 31.698047269231814 23.581275280818005 22.214651638524888 16 21.594735127073832 28.437350690994577 28.49544501330412 21.472469168627466 17 21.24825974204599 28.945617588660195 28.317765304874005 21.488357364419812 18 21.07982781850755 27.959724544826294 30.31907677938816 20.641370857277998 19 23.08449849920212 27.31112277754165 27.81452081424932 21.78985790900691 20 23.169686784928476 27.883246350248953 28.32194016859245 20.62512669623012 21 22.566744809336818 27.258703684674135 27.438033315726905 22.736518190262142 22 22.69927808572772 28.614249250738066 27.521142460521812 21.1653302030124 23 21.892977262586182 28.265594488132308 27.972760523870527 21.868667725410983 24 22.70332014741995 27.280967105584065 28.64777618651231 21.36793656048368 25 22.372629259935266 27.76585174715571 28.240809415189126 21.62070957771989 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 279.0 1 279.0 2 444.5 3 610.0 4 610.0 5 610.0 6 1348.5 7 2087.0 8 2087.0 9 2087.0 10 3237.5 11 4388.0 12 4388.0 13 4388.0 14 7839.5 15 11291.0 16 11291.0 17 11291.0 18 21214.5 19 31138.0 20 31138.0 21 31138.0 22 50423.5 23 69709.0 24 69709.0 25 69709.0 26 99840.0 27 129971.0 28 129971.0 29 129971.0 30 167927.0 31 205883.0 32 205883.0 33 205883.0 34 232266.0 35 258649.0 36 258649.0 37 258649.0 38 276656.0 39 294663.0 40 294663.0 41 294663.0 42 305291.5 43 315920.0 44 315920.0 45 315920.0 46 319076.0 47 322232.0 48 322232.0 49 322232.0 50 306594.5 51 290957.0 52 290957.0 53 290957.0 54 256557.5 55 222158.0 56 222158.0 57 222158.0 58 189709.5 59 157261.0 60 157261.0 61 157261.0 62 126784.5 63 96308.0 64 96308.0 65 96308.0 66 73665.5 67 51023.0 68 51023.0 69 51023.0 70 37038.0 71 23053.0 72 23053.0 73 23053.0 74 16088.5 75 9124.0 76 9124.0 77 9124.0 78 6261.5 79 3399.0 80 3399.0 81 3399.0 82 2256.5 83 1114.0 84 1114.0 85 1114.0 86 696.0 87 278.0 88 278.0 89 278.0 90 176.5 91 75.0 92 75.0 93 75.0 94 51.5 95 28.0 96 28.0 97 28.0 98 23.0 99 18.0 100 18.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3599273429655071 2 0.039694341577604236 3 0.03573689966805457 4 0.03529718390032683 5 0.04049382479165468 6 0.01750868238770459 7 0.01758863070910963 8 0.023185013207462694 9 0.020186951154773553 10 0.01966728706564077 11 0.01866793304807772 12 0.01966728706564077 13 0.01946741626212816 14 0.02746224840263254 15 0.028301705777385496 16 0.020426796118988684 17 0.02198578838638704 18 0.10493217184411996 19 0.02450416051064592 20 0.022185659189899648 21 0.0253835920461014 22 0.019507390422830683 23 0.02158604677936182 24 0.01846806224456511 25 0.022025762547089562 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2501616.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.14547514066012 #Duplication Level Percentage of deduplicated Percentage of total 1 81.95675704711213 57.48895664057325 2 12.155744920109367 17.05341006219474 3 3.1932670970955512 6.719797133304116 4 1.152975122709887 3.235039512313837 5 0.513702251894975 1.8016944270000044 6 0.2872980850342245 1.209159640903647 7 0.163882788870187 0.8046945264873028 8 0.10586066291724183 0.5940517199228149 9 0.07887105369023673 0.4979202782351553 >10 0.3506646900101507 4.250730940346112 >50 0.021750643385272255 1.0640662504509377 >100 0.01568550367360715 2.1896418040622363 >500 0.002169759240248758 1.0659522823281005 >1k 0.0013703742569828022 2.0248847818778204 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 4526 0.18092305133961406 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4077 0.16297465318418175 No Hit GTATCAACGCAGAGTACTTTTTTTT 3924 0.1568586065966959 No Hit CTGTAGGACGTGGAATATGGCAAGA 3906 0.1561390717040505 No Hit CTTTAGGACGTGAAATATGGCGAGG 3800 0.1519018106695832 No Hit GTCCTACAGTGTGCATTTCTCATTT 3206 0.12815715921228518 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.997416070252189E-5 0.0 2 0.0 0.0 0.0 3.997416070252189E-5 0.0 3 0.0 0.0 0.0 3.997416070252189E-5 0.0 4 0.0 0.0 0.0 3.997416070252189E-5 0.0 5 0.0 0.0 0.0 3.997416070252189E-5 0.0 6 0.0 0.0 0.0 3.997416070252189E-5 0.0 7 0.0 0.0 0.0 3.997416070252189E-5 0.0 8 0.0 0.0 0.0 3.997416070252189E-5 0.0 9 0.0 0.0 0.0 7.994832140504378E-5 0.0 10 0.0 0.0 0.0 7.994832140504378E-5 0.0 11 0.0 0.0 0.0 7.994832140504378E-5 0.0 12 0.0 0.0 0.0 1.1992248210756567E-4 3.997416070252189E-5 13 0.0 0.0 0.0 1.1992248210756567E-4 7.994832140504378E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGACT 50 0.0015037094 13.295633 4 TCCAACG 180 0.0 12.669854 18 TGAACCG 85 3.9605384E-6 12.290081 5 TAGGACC 960 0.0 12.266804 4 GGCGAGG 1180 0.0 11.345245 19 GTCCTAA 1320 0.0 11.263677 1 AATAGCG 60 0.005891739 11.079694 5 AGGACCG 95 1.3659639E-5 10.996388 5 GTCCTAC 2760 0.0 10.946611 1 TGTAGGA 2510 0.0 10.821363 2 CAGCGTA 80 3.7908653E-4 10.681855 9 TTATACT 375 0.0 10.636506 4 TCCTACA 2900 0.0 10.545024 2 AATCCCG 345 0.0 10.457823 19 GTCCTAT 320 0.0 10.424316 1 AGGACGT 2385 0.0 10.313174 5 GACGTGA 1410 0.0 10.302653 7 CTGTAGG 2575 0.0 10.289566 1 GGACGTG 2405 0.0 10.264435 6 GTATTAG 270 0.0 10.236789 1 >>END_MODULE