FastQCFastQC Report
Thu 26 May 2016
SRR1512967_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512967_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2501616
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44100.17628604869812153No Hit
GTCCTACAGTGGACATTTCTAAATT43770.1749669013949383No Hit
CTTTAGGACGTGAAATATGGCGAGG39980.15981669448868252No Hit
CTGTAGGACGTGGAATATGGCAAGA38590.154260286151032No Hit
GTCCTAAAGTGTGTATTTCTCATTT37310.14914359358110918No Hit
TATCAACGCAGAGTACTTTTTTTTT31560.1261584511771591No Hit
GTCCTACAGTGTGCATTTCTCATTT30050.12012235291107827No Hit
GGTATCAACGCAGAGTACTTTTTTT28600.1143260996092126No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACCG400.00522022614.2745945
TAGGACC10200.013.1550194
TCCAACG2700.013.00397418
GGCGTGC1103.801506E-812.0945658
CCAACGA2050.012.0353219
CGTGCGC802.8673472E-511.87526210
GGCGAGG10950.011.87256619
GCACCGT907.3481315E-611.6276556
GCCGGTT1657.2759576E-1211.50481611
AATGTCC6650.011.0033258
TGGCGAG25300.010.9146718
AGGACCT17650.010.4060325
GGTATCA14050.010.3557851
TTAGGAC16600.010.253643
AAGGCGT1303.769528E-710.2453466
TAGGACA9950.010.2337124
TGTAGGA29250.010.077272
ATGGCGA27950.010.05179317
GTAGGAC28650.09.9238393
TAGACAG4700.09.921355
Produced by FastQC (version 0.11.5)